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Aliases & Descriptions for UBE2E3
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases EC 6.3.2.19 3 UBCE4 3 UBCH9 2 , 3 , 5 UbcH9 1 , 3 UbcM2 2 , 3
Descriptions Ubiquitin carrier protein E3 3 Ubiquitin-conjugating enzyme E2-23 kDa 3 Ubiquitin-protein ligase E3 3 ubiquitin carrier protein 2 ubiquitin-conjugating enzyme E2E 3 2 ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) 2 ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5) 1 , 2 ubiquitin-protein ligase 2
Search outside databases for aliases for UBE2E3 genePrevious GC identifers: GC02P180033 GC02P180529 GC02P181809 GC02P182047 GC02P181670
Summaries for UBE2E3 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for UBE2E3 : The modification of proteins with ubiquitin is an important cellular mechanism for targetingabnormal or short-lived proteins for degradation. Ubiquitination involves at least three classesof enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, andubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugatingenzyme family. The encoded protein shares 100% sequence identity with the mouse and ratcounterparts, which indicates that this enzyme is highly conserved in eukaryotes. Twoalternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq] UniProtKB/Swiss-Prot: UB2E3_HUMAN, Q969T4 Function : Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in theregulation of transepithelial sodium transport in renal cells. May be involved in cell growtharrest
Gene Wiki entry for UBE2E3
Genomic Location for UBE2E3
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the UBE2E3 gene Entrez Gene cytogenetic band: 2q32.1 Ensembl cytogenetic band: 2q31.3 HGNC cytogenetic band: 2q31.3 UBE2E3 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 2 GeneLoc Exon Structure
GeneLoc location for GC02P181553:
(about GC identifiers )
Start:
181,553,345 bp from pter
End:
181,636,413 bp from pter
Size:
83,069 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000002.10 NT_005403.16 Proteins for UBE2E3
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: UB2E3_HUMAN, Q969T4 (See
protein sequence )Recommended Name: Ubiquitin-conjugating enzyme E2 E3 Size : 207 amino acids; 22913 Da
Subunit : The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to itsimport into the nucleus (By similarity). Interacts with NEDD4L
Subcellular location : Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in aIPO11-dependent manner
Secondary accessions : Q5U0R7 Q7Z4W4
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (2 alternative transcripts):
NP_006348.1 NP_872619.1 ENSEMBL proteins: ENSP00000386788 ENSP00000376215 ENSP00000307109 ENSP00000386364 ENSP00000386445 ENSP00000386798 ENSP00000386825 Human Recombinant Proteins                OriGene Purified Recombinant Human Proteins (see all 2 ): UBE2E3 3 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for UBE2E3: Assays for UBE2E3:
Protein
Domains/ Families for UBE2E3(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q969T4 ProtoNet protein and cluster: Q969T4
1 Blocks protein family : IPB000608 Ubiquitin-conjugating enzymes UniProtKB/Swiss-Prot: UB2E3_HUMAN, Q969T4 Similarity : Belongs to the ubiquitin-conjugating enzyme family
Gene Function for UBE2E3
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
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SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_182678 Applied Biosystems Silencer ® siRNAs for UBE2E3 Sigma-Aldrich siRNA and siRNA Panels for UBE2E3 Sigma-Aldrich shRNA Panels and shRNA for UBE2E3 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_006357                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_006357                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_006357  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_006357 UniProtKB/Swiss-Prot: UB2E3_HUMAN, Q969T4 Function : Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in theregulation of transepithelial sodium transport in renal cells. May be involved in cell growtharrest Catalytic activity : ATP + ubiquitin + protein lysine = AMP + diphosphate + proteinN-ubiquityllysine Enzyme Number (IUBMB): EC 6.3.2.19
Genatlas biochemistry entry for UBE2E3 :ubiquitin conjugating enzyme E2E3,homologous to yeast UBC4/5,expressed in skeletal muscle 1 MGI phenotypic allele for Ube2e3 (no phenotypes)3 Gene Ontology (GO) molecular function terms (links to tree view) :
About this table
Pathways & Interactions for UBE2E3
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for UBE2E3 (Your Favorite Gene powered by Ingenuity) UniProtKB/Swiss-Prot: UB2E3_HUMAN, Q969T4 Pathway : Protein modification; protein ubiquitination
Gene Network CentralTM Interacting Genes and Proteins Network for UBE2E3 5/29 Interacting proteins for UBE2E3 (Q969T4 1 , 2 ENSP00000307109 3 ) via UniProtKB, MINT, and/or STRING (see all 29
)Interactant Interaction Details GeneCard External ID(s) ENSG00000183574 Q99942 1 , 2 EBI-348496, EBI-348482 MINT-62676 MINT-62752 MINT-62578 ENSG00000206254 Q99942 1 , 2 EBI-348496, EBI-348482 MINT-62676 MINT-62752 MINT-62578 RNF5 Q99942 1 , 2 EBI-348496, EBI-348482 MINT-62676 MINT-62752 MINT-62578 EBI-348496, EBI-348482 MINT-62676 MINT-62752 MINT-62578 RNF5 Q99942 1 , 2 EBI-348496, EBI-348482 MINT-62676 MINT-62752 MINT-62578 EBI-348496, EBI-348482 MINT-62676 MINT-62752 MINT-62578 ATXN1 P54253 2 MINT-2868342
About this table 4 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0019941 modification-dependent protein catabolic process
IEA -- GO:0040008 regulation of growth
IEA -- GO:0043687 post-translational protein modification
IEA -- GO:0051246 regulation of protein metabolic process
IEA --
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Drugs & Compounds for UBE2E3 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for UBE2E3 3 Novoseek chemical compound relationships for UBE2E3 gene
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Transcripts for UBE2E3(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
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shRNA from
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OriGene ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_182678 Sigma-Aldrich siRNA and siRNA Panels for UBE2E3 Sigma-Aldrich shRNA Panels and shRNA for UBE2E3 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_006357 NM_182678
REFSEQ mRNAs for UBE2E3 gene (2 alternative transcripts): NM_006357.2 NM_182678.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_006357 NM_182678
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_006357                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_006357                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_006357  
Additional cDNA sequence: AB017644.1 AF085362.1 AK314145.1 BC003554.1 BC003687.1 BC092407.1 BT019345.1 CR593046.1 CR603132.1 CR607535.1 CR616134.1 CR621773.1 CR622410.1 CR624412.1
20 DOTS entries : DT.218837 DT.95227459 DT.99940731 DT.99983990 DT.92451651 DT.100826030 DT.102831523 DT.120950576 DT.95227487 DT.100031738 DT.120950601 DT.100717255 DT.100800490 DT.100826023 DT.92451650 DT.40189481 DT.91824456 DT.91926280 DT.95098679 DT.95227461
24/393 AceView cDNA sequences (see all 393
):AA280089 BG187677 BE504278 AI348453 BU608985 AA731196 BI962902 BC003687 BE887721 AA016236 BG756114 CR603132 AA470054 CR624412 BU178864 BC003554 CB143937 BM847774 BQ020873 T31441 BE018406 BM970528 BQ441123 AB017644
highest scoring ESTs for UBE2E3 :AB017644 AA016236 AA018468 AA020993 AA022626 AA022773 AA025668 AA025669 AA054004 AA059201
Unigene Cluster for UBE2E3: Ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast) Hs.470804 [show with all ESTs ] Unigene Representative Sequence: BM912832 GeneLoc Exon Structure 7 Ensembl transcripts including schematic representations : ENST00000410062
ENST00000392415
ENST00000305934
ENST00000410114
ENST00000409247
ENST00000409513
ENST00000409596
Expression for UBE2E3
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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UBE2E3 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for UBE2E3 1 / 2 / 3
3 probe-sets matching UBE2E3 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: CTCCTGAAGGSOURCE GeneReport for Unigene cluster: Hs.470804 Expression variation in blood from EXPOLDB for UBE2E3
UniProtKB/Swiss-Prot: UB2E3_HUMAN, Q969T4 Tissue specificity : Ubiquitously expressed at low levels. Highly expressed in skeletal muscle
Orthologs for UBE2E3
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for UBE2E3 gene from 5/9 species (see all 9
)
About this table Species with no ortholog for UBE2E3 ENSEMBL Gene Tree for UBE2E3 Paralogs for UBE2E3 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for UBE2E3 gene UBE2E1 2 UBE2E2 2
SNPs/Variants for UBE2E3 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for UBE2E3 (up to first 250kb)
Disorders & Mutations for UBE2E3
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
Novoseek , PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
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OMIM: 604151 4 Novoseek disease relationships for UBE2E3 gene
About this table 1 PharmGKB disease relationship for UBE2E3 gene About this table
Medical News for UBE2E3 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for UBE2E3 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/115 PubMed articles for UBE2E3 gene (see all 115
): cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin- conjugating enzyme. (PubMed id 10343118) 1, 2, 3, 4 Ito K.... Okano Y. (1999) Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import. (PubMed id 15545318) 1, 3, 4 Plafker S.M.... Macara I.G. (2004) Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621) 3, 4 Hillier L.W....Wilson R.K. (2005) Large-scale characterization of HeLa cell nuclear phosphoproteins. (PubMed id 15302935) 3, 4 Beausoleil S.A....Gygi S.P. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) The human ubiquitin conjugating enzyme, UBE2E3, is required for proliferation of retinal pigment epithelial cells. (PubMed id 18614808) 1, 3 Plafker K.S....Plafker S.M. (2008) Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. (PubMed id 10187858) 1, 3 Desterro J.M.P.... Hay R.T. (1999) N-terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. (PubMed id 11322894) 1, 3 Ito K.... Okano Y. (2001) Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348) 3 Barbe L....Andersson-Svahn H. (2008) A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. (PubMed id 16713569) 3 Lim J....Zoghbi H.Y. (2006)
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Genome Databases showing UBE2E3
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Ensembl ,
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ECgene ,
and/or
H-InvDB )
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Other Databases showing UBE2E3
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Specialized Databases showing UBE2E3 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for UBE2E3 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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