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UBE2E2 Gene

protein-coding   GIFtS: 60

GC03P023221
ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
(Previous name: ubiquitin-conjugating enzyme E2E 2 (homologous to yeast UBC4/5) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 6.3.2.19 3
FLJ25157 1, 2
OTTHUMP00000161048 2
UBCH8 2, 3
UbcH8 1, 3
Descriptions
Ubiquitin carrier protein E2 2, 3
Ubiquitin-protein ligase E2 2, 3
ubiquitin-conjugating enzyme E2E 2 2
ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) 2
ubiquitin-conjugating enzyme E2E 2 (homologous to yeast
UBC4/5) 1, 2
External Ids
HGNC: 124781
Entrez Gene: 73252
UniProtKB: Q96LR53
Ensembl: ENSG000001822477
Search outside databases for aliases for UBE2E2 gene

Previous GC identifers: GC03U990078 GC03P023220

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: UB2E2_HUMAN, Q96LR5
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (Probable)

Gene Wiki entry for UBE2E2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2E2 gene  

Entrez Gene cytogenetic band: 3p24.2   Ensembl cytogenetic band:  3p24.3   HGNC cytogenetic band: 3p24.2

UBE2E2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P023221:     (about GC identifiers)

Start:
23,219,788 bp from pter
End:
23,608,288 bp from pter
Size:
388,501 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_022517.17  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: UB2E2_HUMAN, Q96LR5 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 E2  
Size: 201 amino acids; 22255 Da
PDB structures from and Proteopedia :
1Y6L (3D)    

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_689866.1  

    ENSEMBL proteins: 
    ENSP00000379931 ENSP00000338340 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Millipore Purified and/or Recombinant UBE2E2 Protein
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for UBE2E2
    Recombinant Proteins from Abcam (UBE2E2)
    Human Recombinant Proteins from Abnova (UBE2E2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for UBE2E2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for UBE2E2
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    Antibodies from Abcam (UBE2E2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (UBE2E2)
    Novus Biologicals Antibody for UBE2E2

    Assays for UBE2E2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Enzo Life Sciences assays for UBE2E2

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000608 UBQ-conjugat_E2
     IPR016135 UBQ-conjugat/RWD-like


       GeneDecks  UBE2E2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q96LR5

    ProtoNet protein and cluster: Q96LR5

    1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

    UniProtKB/Swiss-Prot: UB2E2_HUMAN, Q96LR5
    Similarity: Belongs to the ubiquitin-conjugating enzyme family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2E2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2E2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152653

                  Applied Biosystems Silencer® siRNAs for UBE2E2

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2E2  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2E2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for UBE2E2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_152653
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_152653
                                     untagged cDNA clone in CMV expression vector: NM_152653 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_152653

    UniProtKB/Swiss-Prot: UB2E2_HUMAN, Q96LR5
    Function: Catalyzes the covalent attachment of ubiquitin to other proteins (Probable)
    Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
    N-ubiquityllysine
    Enzyme Number (IUBMB): EC 6.3.2.19 

    Genatlas biochemistry entry for UBE2E2:
    ubiquitin conjugating enzyme E2E2,homologous to yeast UBC4/5,ubiquitously expressed, involved in
    selective protein degradation

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842 ubiquitin-protein ligase activity IEA--
    GO:0016874 ligase activity IEA--
    GO:0042296 ISG15 ligase activity IDA16428300
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for UBE2E2
     Common schema of ubiquitination

       GeneDecks  UBE2E2 for the pathways selected above  
    About GeneDecksing

    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2E2  (Your Favorite Gene powered by Ingenuity) 
     Hypoxia Signaling in the Cardiovascular System
     Protein Ubiquitination Pathway

       GeneDecks  UBE2E2 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for UBE2E2):
     hsa04120 Ubiquitin mediated proteolysis

       GeneDecks  UBE2E2 for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: UB2E2_HUMAN, Q96LR5
    Pathway: Protein modification; protein ubiquitination

     Gene Network CentralTM Interacting Genes and Proteins Network for  UBE2E2 


    5/24 Interacting proteins for UBE2E2 (ENSP000003383403) via UniProtKB, MINT, and/or STRING (see all 24 )

    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBA1ENSP000003384133STRING (score=.941)
    UBE2L3ENSP000003442593STRING (score=.906)
    PARK2ENSP000003558653STRING (score=.903)
    PPFIA2ENSP000003274163STRING (score=.899)
    SUMO1ENSP000002642813STRING (score=.899)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0019941 modification-dependent protein catabolic process IEA--
    GO:0032020 ISG15-protein conjugation IDA16428300
    GO:0051246 regulation of protein metabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBE2E2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBE2E2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (UBE2E2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152653

                  Sigma-Aldrich siRNA and siRNA Panels for UBE2E2  
                         Sigma-Aldrich shRNA Panels and shRNA for UBE2E2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_152653  

    REFSEQ mRNAs for UBE2E2 gene: 

    NM_152653.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_152653  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_152653
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_152653
                                     untagged cDNA clone in CMV expression vector: NM_152653 

    Additional cDNA sequence: 

    AK057886.1 AK307075.1 BC022332.1 CR592620.1 CR615770.1 CR617055.1 

    3 DOTS entries:

    DT.315038  DT.95125033  DT.100754439 

    24/152 AceView cDNA sequences (see all 152 ):

    CB850618 Z44894 CR617055 CK905445 AA296821 BM716302 AA766277 F04757 
    CR592620 BQ888699 CD675501 F03864 BM674560 CF141387 BM688855 H64990 
    AW960528 Z38774 BM701561 BI495063 CA418581 AA099871 BF002750 BM504079 

    highest scoring ESTs for UBE2E2:

    AI432317 AA099336 AA099871 AA125780 AA297234 AA766277 AA858294 AA890608 AI216404 AI377670 

    Unigene Cluster for UBE2E2:

    Ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
    Hs.475688  [show with all ESTs]
    Unigene Representative Sequence: NM_152653


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000396703  ENST00000335798  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBE2E2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBE2E2

    1 / 2 / 3

    4 probe-sets matching UBE2E2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    48087_at2, 3 U95-B 1 1.00 1.00 0.74 1.44 AI432317 1.00 1.00 1.00 1

    42082_at2 U95-B 1 1.00 1.00 0.70 0.57 -- -- -- -- --

    225651_at2, 3 U133-B 1 1.00 1.00 -- -- BF431962 0.60 1.00 0.82 1

    225651_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTACTGGGAA

    SOURCE GeneReport for Unigene cluster: Hs.475688
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBE2E2 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    UBE2E21   -- ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, more 95.17(n)
    97(a)
    485477  XM_847335.1  XP_852428.1 
    chimpanzee
    (Pan troglodytes)
    UBE2E21   -- ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, more 99.83(n)
    100(a)
    460225  XM_530592.2  XP_530592.2 
    cow
    (Bos taurus)
    UBE2E21   -- ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, more 91.87(n)
    99.5(a)
    784326  XM_001251967.1  XP_001251968.1 
    rat
    (Rattus norvegicus)
    Ube2e21   -- ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, more 91.54(n)
    100(a)
    361013  XM_341288.3  XP_341289.3 
    mouse
    (Mus musculus)
    Ube2e21, 5 145
    ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, more1, 5 91.87(n)1
    99.5(a)1
    2187931  NM_144839.11  NP_659088.11 
     AK1392835  AK1626025  (see all 8)
    About this table        Species with no ortholog for UBE2E2

    ENSEMBL Gene Tree for UBE2E2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for UBE2E2 gene
    UBE2E32  UBE2E12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/2141 NCBI SNPs in UBE2E2 are shown (see all 2141 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 627)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs76317051,2
    F,H23607238(+) AGCTGT/CCAGTA 1 -- ut314Minor allele frequency- C:0.35EU EA WA 420
    rs119158701,2
    H23218477(+) ATTTTG/CAACTT 1 -- ng514Minor allele frequency- C:0.00EU EA WA 398
    rs67864671,2
    H23607274(+) GTAAAT/CTGCTA 1 -- ut314Minor allele frequency- C:0.00EU EA WA 418
    rs14988091,2
    A,C,F,H,O23536954(-) GTAATG/CATATT 1 -- int14Minor allele frequency- C:0.41EU NA 252
    rs31113111,2
    A,C,F,H,O23500296(+) GGATCC/TAGGCC 1 -- int111Minor allele frequency- T:0.34EU EA WA NA 978
    rs21321481,2
    A,C,F,H,O23246745(-) CCTCAC/AAGTCT 1 -- int116Minor allele frequency- A:0.19NS EU EA WA NA 1084
    rs7785011,2
    A,C,F,H,O23449871(-) AGCACA/GCATAA 1 -- int17Minor allele frequency- G:0.36NA EU EA WA 550
    rs18749131,2
    A,C,F,H,O23564037(-) ACCAAT/CAACAA 1 -- int19Minor allele frequency- C:0.39EU EA WA NA 718
    rs7784721,2
    A,C,F,H,O23480903(-) TTGATG/ATTATG 1 -- int18Minor allele frequency- A:0.44EU EA WA 838
    rs7785241,2
    A,C,F,H,O23469516(-) TACTAT/CTAAAA 1 -- int110Minor allele frequency- C:0.31NA EA EU WA 797
    About this table

    HapMap Linkage Disequilibrium images for UBE2E2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 602163

    1 Novoseek disease relationship for UBE2E2 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    parkinson disease 14.07 1 12730996 (1)
    About this table

    Human Genome Epidemiology Navigator: UBE2E2 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/33 PubMed articles for UBE2E2 gene (see all 33 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 7325 HGNC: 12478 AceView: UBE2E2 Ensembl:ENSG00000182247 euGenes: HUgn7325
    ECgene: UBE2E2 H-InvDB: UBE2E2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBE2E2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for UBE2E2 Antibodies for UBE2E2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for UBE2E2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
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     Search OriGene for UBE2E2
     Search Tocris compounds for UBE2E2




     Assays/Proteins for UBE2E2
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for UBE2E2

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