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UBE2D4 Gene

protein-coding   GIFtS: 53

GC07P043932
ubiquitin-conjugating enzyme E2D 4 (putative)
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.3.2.19 3
HBUCE1 1, 2, 3
Descriptions
Ubiquitin carrier protein D4 3
Ubiquitin-protein ligase D4 3
ubiquitin-conjugating enzyme E2D 4 (putative) 2
ubiquitin-conjugating enzyme HBUCE1 2
External Ids
HGNC: 216471
Entrez Gene: 516192
UniProtKB: Q9Y2X83
Ensembl: ENSG000000789677
Search outside databases for aliases for UBE2D4 gene

Previous GC identifer: GC07P043740

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: UB2D4_HUMAN, Q9Y2X8
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBE2D4 gene  

Entrez Gene cytogenetic band: 7p13   Ensembl cytogenetic band:  7p13   HGNC cytogenetic band: 7p13

UBE2D4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07P043932:     (about GC identifiers)

Start:
43,932,572 bp from pter
End:
43,959,688 bp from pter
Size:
27,117 bases
Orientation:
plus strand

1 alternative location:
Chr7+,CRA_TCAG 44,005,548-44,032,657     
RefSeq DNA sequence:
NC_000007.12  NT_007819.16  NT_079592.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: UB2D4_HUMAN, Q9Y2X8 (See protein sequence)
Recommended Name: Ubiquitin-conjugating enzyme E2 D4  
Size: 147 amino acids; 16649 Da

REFSEQ proteins: NP_057067.1  

ENSEMBL proteins: 
ENSP00000222402 


Human Recombinant Proteins 
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Browse Purified and Recombinant Proteins at Millipore
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Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (UBE2D4)
Human Recombinant Proteins from Abnova (UBE2D4)
              OriGene Purified Recombinant Human Protein: UBE2D4 


Antibodies for UBE2D4: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for UBE2D4
Browse R&D Systems for Antibodies
Antibodies from Abcam (UBE2D4), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (UBE2D4)
Novus Biologicals Antibody for HBUCE1

Assays for UBE2D4: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR000608 UBQ-conjugat_E2
 IPR016135 UBQ-conjugat/RWD-like


   GeneDecks  UBE2D4 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9Y2X8

ProtoNet protein and cluster: Q9Y2X8

1 Blocks protein family: IPB000608 Ubiquitin-conjugating enzymes

UniProtKB/Swiss-Prot: UB2D4_HUMAN, Q9Y2X8
Similarity: Belongs to the ubiquitin-conjugating enzyme family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2D4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2D4)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015983

              Applied Biosystems Silencer® siRNAs for UBE2D4

              Sigma-Aldrich siRNA and siRNA Panels for UBE2D4  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2D4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for UBE2D4
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015983
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015983
                                 untagged cDNA clone in CMV expression vector: NM_015983 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_015983

UniProtKB/Swiss-Prot: UB2D4_HUMAN, Q9Y2X8
Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity)
Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein
N-ubiquityllysine
Enzyme Number (IUBMB): EC 6.3.2.19 

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004842 ubiquitin-protein ligase activity IEA--
GO:0005515 protein binding IPI16189514
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  UBE2D4  (Your Favorite Gene powered by Ingenuity) 
 Hypoxia Signaling in the Cardiovascular System
 Protein Ubiquitination Pathway

   GeneDecks  UBE2D4 for the pathways selected above  
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1 Kegg Pathway  (Kegg details for UBE2D4):
 hsa04120 Ubiquitin mediated proteolysis

   GeneDecks  UBE2D4 for the pathways selected above  
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UniProtKB/Swiss-Prot: UB2D4_HUMAN, Q9Y2X8
Pathway: Protein modification; protein ubiquitination


5/24 Interacting proteins for UBE2D4 (Q9Y2X81, 2 ENSP000002224023) via UniProtKB, MINT, and/or STRING (see all 24 )

InteractantInteraction Details
GeneCardExternal ID(s)
DTX2Q86UW91, 2EBI-745527, EBI-740376 MINT-67411 EBI-745527, EBI-740376 MINT-67411
DTX2Q86UW91, 2EBI-745527, EBI-740376 MINT-67411 EBI-745527, EBI-740376 MINT-67411
ENSG00000204599Q9HCM92MINT-67140
ENSG00000206495Q9HCM92MINT-67140
MAGEA11P433642MINT-66676
About this table

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0019941 modification-dependent protein catabolic process IEA--
GO:0043687 post-translational protein modification IEA--
GO:0051246 regulation of protein metabolic process IEA--
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for UBE2D4

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (UBE2D4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (UBE2D4)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_015983

              Sigma-Aldrich siRNA and siRNA Panels for UBE2D4  
                     Sigma-Aldrich shRNA Panels and shRNA for UBE2D4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_015983  

REFSEQ mRNAs for UBE2D4 gene: 

NM_015983.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_015983  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_015983
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_015983
                                 untagged cDNA clone in CMV expression vector: NM_015983 

Additional cDNA sequence: 

AF125044.1 AF125045.1 AK001446.1 BC004104.2 CR608137.1 

11 DOTS entries:

DT.80102034  DT.100019767  DT.100776196  DT.121041056  DT.100776192  DT.100776194  DT.405831  DT.100776195 
DT.95370728  DT.100804099  DT.91746835 

24/199 AceView cDNA sequences (see all 199 ):

BM702851 BU933711 BQ931663 AW612621 BQ892318 BQ226554 AF125045 BI908286 
BG057654 BM717478 BM808115 BM922789 BM681664 NM_015983 AK001446 BC004104 
AA366525 AI911590 AI025707 AI434404 BE467880 AA350152 BE855796 BI962825 

highest scoring ESTs for UBE2D4:

AA045185 AA056016 AA190487 AA350151 AA350152 AA514438 AA527399 AA527790 AA569028 AA643161 

Unigene Cluster for UBE2D4:

Ubiquitin-conjugating enzyme E2D 4 (putative)
Hs.19196  [show with all ESTs]
Unigene Representative Sequence: BM543814


GeneLoc Exon Structure

5/7 Alternative Splicing Database (ASD) splice patterns (SP) for UBE2D4 (see all 7 )

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c · 6d ^ 7 ^ 8a · 8b · 8c · 8d · 8e
SP1:                          -                       -     -           -     -                                       
SP2:                          -                             -           -     -                                       
SP3:                                                  -     -                                                         
SP4:                          -           -           -     -           -     -                                       
SP5:                          -           -     -     -     -           -     -                                       

About this scheme

ECgene alternative splicing isoforms for UBE2D4

1 Ensembl transcript including schematic representation:
ENST00000222402  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

UBE2D4 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for UBE2D4

1 / 2 / 3

6 probe-sets matching UBE2D4 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

65521_at2, 3 U133-C 1 1.00 1.00 0.89 1.28 W74577 0.80 1.00 0.91 1
57309_f_at2, 3 U95-C 1 0.75 1.00 0.99 0.74 AA045185 1.00 1.00 1.00 1

65521_at2, 3 U133-A 1 1.00 1.00 -- -- W74577 0.80 1.00 0.91 1

218837_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_015983 0.60 1.00 0.82 1

65521_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

218837_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  UBE2D4 for binary patterns associated with the probe-sets selected above  
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About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTACTCCATT

SOURCE GeneReport for Unigene cluster: Hs.19196
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for UBE2D4 gene from 5/14 species (see all 14 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
UBE2D41   -- ubiquitin-conjugating enzyme E2D 4 (putative) 99.32(n)
100(a)
463379  XM_519070.2  XP_519070.2 
cow
(Bos taurus)
UBE2D41   -- ubiquitin-conjugating enzyme E2D 4 (putative) 90.8(n)
99.25(a)
511801  NM_001075539.1  NP_001069007.1 
rat
(Rattus norvegicus)
RGD694251   -- ubiquitin-conjugating enzyme 77.1(n)
87.07(a)
79435  NM_031001.2  NP_112263.1 
mouse
(Mus musculus)
OTTMUSG000000207331   -- predicted gene, OTTMUSG00000020733 75.28(n)
85.71(a)
100041484  XM_001476705.1  XP_001476755.1 
zebrafish
(Danio rerio)
ube2d11   -- ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, more 80.5(n)
95.92(a)
324015  NM_199664.1  NP_955958.1 
About this table        Species with no ortholog for UBE2D4

ENSEMBL Gene Tree for UBE2D4
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for UBE2D4 gene
UBE2D3P2  UBE2D12  UBE2D22  

Pseudogenes
3 related pseudogenes


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/133 NCBI SNPs in UBE2D4 are shown (see all 133 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 46)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 7 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs10454391,2
C,F,H43959495(+) GTTTTT/CAGAAG 1 -- ut31 ese312Minor allele frequency- C:0.09MN NA EU EA WA 1164
rs10453981,2
F,H,O43958951(+) ACCCAC/GTCTCT 1 -- ut31 ese35Minor allele frequency- G:0.12MN EU EA WA 590
rs25278121,2
A,C,F,H,O43950562(+) TTGCTA/GTGTCT 1 -- int116Minor allele frequency- N:0.00EU EA WA NA 1418
rs25278131,2
A,C,F,H,O43950779(+) CTTTTA/GGCAAG 1 -- int114Minor allele frequency- N:0.00EA NA EU WA 1238
rs22562941,2
A,C,F,H,O43954527(-) GAACAC/TAGGGG 1 -- int18Minor allele frequency- T:0.48EA EU WA NA 2030
rs25956441,2
A,C,F,H,O43947065(-) GAACAA/CCTTTA 1 -- int117Minor allele frequency- N:0.00NS EU EA WA NA 1146
rs25956471,2
C,F,H,O43943159(-) CACGTG/ATGGAT 1 -- int116Minor allele frequency- A:0.06EU EA WA NA 1474
--
rs20793901,2
A,C,F,H43935933(-) caggaC/Attgct 1 -- int14Minor allele frequency- A:0.31EU EA WA 412
--
rs25278091,2
A,C,F,H43942126(+) cctgaA/Gtagct 1 -- int14Minor allele frequency- G:0.33EU EA WA 404
rs18596201,2
C,F,H,O43954474(+) ACCACC/GCTTGT 1 -- int113Minor allele frequency- G:0.09MN EU EA WA NA 1230
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HapMap Linkage Disequilibrium images for UBE2D4 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
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  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

7 PubMed articles for UBE2D4 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 51619 HGNC: 21647 AceView: LOC51619 Ensembl:ENSG00000078967 euGenes: HUgn51619
ECgene: UBE2D4 H-InvDB: UBE2D4
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for UBE2D4:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for UBE2D4-related products

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 Millipore Preclinical / Clinical Development Services
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 Predesigned and custom siRNAs for UBE2D4 Antibodies for UBE2D4
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for UBE2D4 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for UBE2D4




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for HBUCE1

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