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UBA5 Gene

protein-coding   GIFtS: 53

GC03P133862
ubiquitin-like modifier activating enzyme 5
(Previous name: ubiquitin-activating enzyme E1-domain containing 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: UBE1DC1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
FLJ17281 2
FLJ23251 1, 2
FLJ23251UBA5 2
THIFP1 2
ThiFP1 3
UBE1DC1 2, 3, 5
Descriptions
UBA5, ubiquitin-activating enzyme E1 homolog 2
UFM1-activating enzyme 2, 3
Ubiquitin-activating enzyme 5 2, 3
Ubiquitin-activating enzyme E1 domain-containing protein 1 3
ubiquitin-activating enzyme E1-domain containing 1 1, 2
ubiquitin-like modifier activating enzyme 5 2
External Ids
HGNC: 232301
Entrez Gene: 798762
UniProtKB: Q9GZZ93
Ensembl: ENSG000000813077
Search outside databases for aliases for UBA5 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for UBA5:
This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein
activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the
formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding
distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also
been identified. [provided by RefSeq]

UniProtKB/Swiss-Prot: UBA5_HUMAN, Q9GZZ9
Function: E1-like enzyme which activates UFM1

Gene Wiki entry for UBA5 (UBE1DC1)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q22.1   Ensembl cytogenetic band:  3q22.1   HGNC cytogenetic band: 3q22

UBA5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P133862:     (about GC identifiers)

Start:
133,861,836 bp from pter
End:
133,879,312 bp from pter
Size:
17,477 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000003.10  NT_005612.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: UBA5_HUMAN, Q9GZZ9 (See protein sequence)
Recommended Name: Ubiquitin-like modifier-activating enzyme 5  
Size: 404 amino acids; 44863 Da
Secondary accessions: Q96ST1

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_079094.1  NP_938143.1  


    ENSEMBL proteins: 
    ENSP00000348565 ENSP00000376839 ENSP00000264991 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (UBA5)
    Human Recombinant Proteins from Abnova (UBE1DC1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IDA11256614
    About this table

    Antibodies for UBA5: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for UBA5
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (UBA5), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (UBE1DC1)
    Novus Biologicals Antibodies for UBA5

    Assays for UBA5: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000594 ThiF_NAD_FAD_bd
     IPR016040 NAD(P)-bd_dom


       GeneDecks  UBA5 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9GZZ9

    ProtoNet protein and cluster: Q9GZZ9

    1 Blocks protein family: IPB000594 UBA/THIF-type NAD/FAD binding fold

    UniProtKB/Swiss-Prot: UBA5_HUMAN, Q9GZZ9
    Similarity: Belongs to the ubiquitin-activating E1 family. UBA5 subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBA5)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_198329

                  Applied Biosystems Silencer® siRNAs for UBA5

                  Sigma-Aldrich siRNA and siRNA Panels for UBA5  
                         Sigma-Aldrich shRNA for UBA5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for UBA5
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_024818
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_024818
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_024818 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_024818

    UniProtKB/Swiss-Prot: UBA5_HUMAN, Q9GZZ9
    Function: E1-like enzyme which activates UFM1

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003824 catalytic activity IEA--
    GO:0005488 binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    5/8 Interacting proteins for UBA5 (ENSP000003485653 Q9GZZ92) via UniProtKB, MINT, and/or STRING (see all 8 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    APEHP137982MINT-67134
    GABARAPL2P605202MINT-67436
    UFC1ENSP000003569823STRING (score=.96)
    UFM1ENSP000003689703STRING (score=.96)
    UBE2MENSP000002530233STRING (score=.834)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008152 metabolic process IEA--
    GO:0019941 modification-dependent protein catabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBA5

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBA5)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_198329

                  Sigma-Aldrich siRNA and siRNA Panels for UBA5  
                         Sigma-Aldrich shRNA for UBA5  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_024818  NM_198329  

    REFSEQ mRNAs for UBA5 gene (2 alternative transcripts): 

    NM_024818.3   NM_198329.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_024818  NM_198329  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_024818
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_024818
                                     untagged cDNA clones in CMV expression vector (see all 3): NM_024818 

    Additional cDNA sequence: 

    AB154406.1 AK026904.1 AK027563.1 AK056238.1 AK129616.1 AL136757.1 AY253672.1 BC009737.1 
    BC048015.1 BX537742.1 CR615958.1 

    18 DOTS entries:

    DT.97780080  DT.113449  DT.100794372  DT.101982431  DT.95357113  DT.95203841  DT.120924343  DT.99980399 
    DT.97843257  DT.97843254  DT.120924319  DT.120924352  DT.120944529  DT.91742338  DT.95163642  DT.120924360 
    DT.92432853  DT.95163640 

    24/191 AceView cDNA sequences (see all 191 ):

    BM714610 AW169967 AI570379 BQ576131 BM504192 AI799509 AL707384 AI473675 
    AA348650 AA433980 BM697556 BX495490 AL136757 AB154406 BG617059 CR615958 
    AA902992 CA414904 AA452533 CB150461 BU071370 BE140268 CD679386 NM_198329 

    Unigene Cluster for UBA5:

    Ubiquitin-like modifier activating enzyme 5
    Hs.170737  [show with all ESTs]
    Unigene Representative Sequence: NM_024818


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for UBA5 (see all 7 )

    ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b ^ 11 ^ 12 ^ 13
    SP1:                    -     -                 -                                                                                       
    SP2:                                -     -     -                                                                                       
    SP3:                                            -                                                                                       
    SP4:                                            -                                                                                       
    SP5:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for UBA5

    3 Ensembl transcripts including schematic representations:
    ENST00000356232  ENST00000393131  ENST00000264991  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBA5 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBA5

    1 / 2

    8 probe-sets matching UBA5 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    54752_at2 U95-B 1 1.00 1.00 0.96 0.98 -- -- -- -- --

    54753_at2 U95-B 2 1.00 0.84 0.96 1.02 -- -- -- -- --

    222579_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

    218289_s_at2 U133-A 2 1.00 0.95 -- -- -- -- -- -- --

    222578_s_at2 U133-B 2 1.00 0.77 -- -- -- -- -- -- --

    222579_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    218289_s_at2 U133Plus2 2 1.00 0.95 -- -- -- -- -- -- --

    222578_s_at2 U133Plus2 2 1.00 0.77 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GGGACGCCCG

    SOURCE GeneReport for Unigene cluster: Hs.170737
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBA5 gene from 5/20 species (see all 20 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    UBA51   -- ubiquitin-like modifier activating enzyme 5 93.32(n)
    96.04(a)
    485664  XM_542784.2  XP_542784.2 
    chimpanzee
    (Pan troglodytes)
    UBA51   -- ubiquitin-like modifier activating enzyme 5 99.34(n)
    99.5(a)
    460705  XM_516757.2  XP_516757.2 
    cow
    (Bos taurus)
    UBA51   -- ubiquitin-like modifier activating enzyme 5 92.99(n)
    94.55(a)
    509292  XM_586224.3  XP_586224.2 
    rat
    (Rattus norvegicus)
    Uba51   -- ubiquitin-like modifier activating enzyme 5 86.07(n)
    88.56(a)
    300968  NM_001009669.1  NP_001009669.1 
    mouse
    (Mus musculus)
    Uba51, 5 95
    ubiquitin-like modifier activating enzyme 51, 5 84.83(n)1
    87.06(a)1
    666631  NM_025692.21  NP_079968.11 
     AA9588785  AK0111515  (see all 20)
    About this table        Species with no ortholog for UBA5

    ENSEMBL Gene Tree for UBA5
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/50 NCBI SNPs in UBA5 are shown (see all 50 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 36)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 3 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10583311,2
    C,F,H,O133878501(+) CATCTG/TCTCAT 2 -- ut3114Minor allele frequency- T:0.47EA MN EU WA NA 2712
    rs14561181,2
    A,C,F,H,O133868841(+) ccacgC/Acattt 2 -- int17Minor allele frequency- A:0.29EU EA WA NA 562
    rs13788071,2
    A,C,F,H,O133867880(+) GGAAAG/CAAGGC 2 -- int113Minor allele frequency- C:0.47NS MN NA EU EA WA 852
    rs124958621,2
    A,C,F,H133873026(+) TCCTGC/AAATGA 2 -- int18Minor allele frequency- A:0.34EU EA WA NA 780
    rs23056291,2
    A,C,F133877246(+) GGCAGC/TATAAA 2 -- int13Minor allele frequency- T:0.40NA 118
    rs67925111,2
    C,F,H133864830(+) cactgT/Cgggta 2 -- int14Minor allele frequency- C:0.02EU EA WA 420
    rs126355751,2
    A,C,F133877474(+) ATATGA/TTTTAC 2 -- int14Minor allele frequency- T:0.46NA EA 362
    rs168394931,2
    C,F,H133864289(+) AGATTA/CGAAAA 2 -- int111Minor allele frequency- C:0.04NA EU EA WA 980
    rs67863051,2
    C,F,H133866678(+) ATTCAC/TGTAAT 2 -- int14Minor allele frequency- T:0.09EU EA WA 420
    rs168395071,2
    F,H133877794(+) GTAAAC/TCAAAA 2 -- int18Minor allele frequency- N:0.00NA EU EA WA 608
    About this table

    HapMap Linkage Disequilibrium images for UBA5 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 610552

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for UBA5 gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 79876 HGNC: 23230 AceView: UBE1DC1 Ensembl:ENSG00000081307 euGenes: HUgn79876
    ECgene: UBA5 H-InvDB: UBA5
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBA5:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for UBA5-related products

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     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for UBA5 Antibodies for UBA5
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for UBA5 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Antibodies for UBA5

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