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UBA2 Gene

protein-coding   GIFtS: 63

GC19P039611
ubiquitin-like modifier activating enzyme 2
(Previous name: SUMO1 activating enzyme subunit 2 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: SAE2)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
ARX 1, 2
EC 6.3.2.- 3
FLJ13058 1, 2
HRIHFB2115 1, 2
SAE2 2, 3
UBLE1B 3
Descriptions
Anthracycline-associated resistance ARX 3
SUMO-1 activating enzyme subunit 2 2
SUMO1 activating enzyme subunit 2 1, 2
UBA2, ubiquitin-activating enzyme E1 homolog 2
Ubiquitin-like 1-activating enzyme E1B 3
anthracycline-associated resistance 2
ubiquitin-like modifier activating enzyme 2 2
External Ids
HGNC: 306611
Entrez Gene: 100542
UniProtKB: Q9UBT23
Ensembl: ENSG000001262617
Search outside databases for aliases for UBA2 gene

Previous GC identifers: GC19P035536 GC19P035368 GC19P039595

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: SAE2_HUMAN, Q9UBT2
Function: The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It
mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved
cysteine residue on SAE2

Gene Wiki entry for UBA2 (SAE2)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the UBA2 gene  

Entrez Gene cytogenetic band: 19q12   Ensembl cytogenetic band:  19q13.11   HGNC cytogenetic band: 19q13.11

UBA2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P039611:     (about GC identifiers)

Start:
39,611,108 bp from pter
End:
39,652,638 bp from pter
Size:
41,531 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011109.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SAE2_HUMAN, Q9UBT2 (See protein sequence)
Recommended Name: SUMO-activating enzyme subunit 2  
Size: 640 amino acids; 71224 Da
Subunit: Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2
Subcellular location: Nucleus
PDB structures from and Proteopedia :
1Y8Q (3D)    1Y8R (3D)    2PX9 (3D)    
Secondary accessions: O95605 Q59H87 Q6IBP6 Q9NTJ1 Q9UED2

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005490.1  

    ENSEMBL proteins: 
    ENSP00000246548 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Millipore Purified and/or Recombinant UBA2 Protein
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for UBA2
    Recombinant Proteins from Abcam (AOS1+UBA2, SAE2 / UBA2)
    Human Recombinant Proteins from Abnova (UBA2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for UBA2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for UBA2
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (AOS1+UBA2, SAE2 / UBA2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (UBA2)
    Novus Biologicals Antibodies for UBA2

    Assays for UBA2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Enzo Life Sciences assays for UBA2

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR018074 UBQ-activ_enz_E1_AS
     IPR000594 ThiF_NAD_FAD_bd
     IPR000127 UBact_repeat
     IPR019572 Ubiquitin-activating_enzyme


       GeneDecks  UBA2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9UBT2

    ProtoNet protein and cluster: Q9UBT2

    1 Blocks protein family: IPB000594 UBA/THIF-type NAD/FAD binding fold

    UniProtKB/Swiss-Prot: SAE2_HUMAN, Q9UBT2
    Similarity: Belongs to the ubiquitin-activating E1 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBA2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005499

                  Applied Biosystems Silencer® siRNAs for UBA2

                  Sigma-Aldrich siRNA and siRNA Panels for UBA2  
                         Sigma-Aldrich shRNA for UBA2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for UBA2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005499
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005499
                                     untagged cDNA clone in CMV expression vector: NM_005499 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005499

    UniProtKB/Swiss-Prot: SAE2_HUMAN, Q9UBT2
    Function: The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It
    mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved
    cysteine residue on SAE2
    Enzyme Number (IUBMB): EC 6.3.2.- 

    Genatlas biochemistry entry for UBA2:
    sentrin (UBL1) avtivating protein component with AOS1(SAE1) and UBL1 required for activation of
    UBL1
    1 MGI phenotypic allele for Uba2 (no phenotypes)

    5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0005524 ATP binding IEA--
    GO:0008047 enzyme activator activity TAS10187858
    GO:0008134 transcription factor binding IEA--
    GO:0008641 small protein activating enzyme activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    1 Invitrogen iPath™ Online BioAtlas - Pathway for UBA2 (Maps provided by GeneGo):
     Putative SUMO-1 pathway

       GeneDecks  UBA2 for the pathways selected above  
    About GeneDecksing

    2 Millipore Pathways for UBA2
     Proteolysis Putative SUMO-1 pathway
     DNA damage Role of SUMO in p53 regulation

       GeneDecks  UBA2 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for UBA2):
     hsa04120 Ubiquitin mediated proteolysis

       GeneDecks  UBA2 for the pathways selected above  
    About GeneDecksing
    UniProtKB/Swiss-Prot: SAE2_HUMAN, Q9UBT2
    Pathway: Protein modification; protein sumoylation

     Gene Network CentralTM Interacting Genes and Proteins Network for  UBA2 


    5/16 Interacting proteins for UBA2 (ENSP000002465483 Q9UBT21, 2) via UniProtKB, MINT, and/or STRING (see all 16 )

    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBE2IP632791STRING (score=.999) EBI-718569, EBI-80168
    SAE1Q9UBE01, 2STRING (score=.999) EBI-718569, EBI-743154 MINT-66320 EBI-718569, EBI-743154 MINT-66320
    SAE1Q9UBE01, 2STRING (score=.999) EBI-718569, EBI-743154 MINT-66320 EBI-718569, EBI-743154 MINT-66320
    BXDC2Q8TDN62MINT-65042
    SUMO1ENSP000002642813STRING (score=.999)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006464 protein modification process IEA--
    GO:0019941 modification-dependent protein catabolic process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for UBA2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (UBA2)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005499

                  Sigma-Aldrich siRNA and siRNA Panels for UBA2  
                         Sigma-Aldrich shRNA for UBA2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005499  

    REFSEQ mRNAs for UBA2 gene: 

    NM_005499.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005499  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005499
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005499
                                     untagged cDNA clone in CMV expression vector: NM_005499 

    Additional cDNA sequence: 

    AB015337.1 AB208872.1 AF079566.1 AF090384.1 AF110957.1 AK023120.1 AK024114.1 AK124730.1 
    AK299514.1 AK315521.1 AL136905.1 BC003153.1 BT009781.1 CR456756.1 U35832.1 

    24/30 DOTS entries (see all 30 ):

    DT.417687  DT.91769032  DT.95371608  DT.100827743  DT.100647308  DT.95371580  DT.101984590  DT.100827746 
    DT.121476967  DT.100838603  DT.100827747  DT.121476497  DT.40225546  DT.91769042  DT.100827744  DT.91877659 
    DT.95184015  DT.100827735  DT.100838962  DT.121398493  DT.121476622  DT.121476680  DT.121476978  DT.86855221 

    24/478 AceView cDNA sequences (see all 478 ):

    BM702283 CB151012 BG257748 AW768255 BF436280 AF110957 BU145315 AA553714 
    BM806947 BF969748 CA393607 BQ213486 CB151240 AA693705 AW449744 BQ889474 
    AI862986 T16287 BE349506 BQ889284 AU124381 BM477166 BP338277 BM472688 

    Unigene Cluster for UBA2:

    Ubiquitin-like modifier activating enzyme 2
    Hs.631580  [show with all ESTs]
    Unigene Representative Sequence: AK124730


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for UBA2

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14a · 14b ^ 15 ^ 16
    SP1:                                                                                                                  
    SP2:                                                                                -                                 
    SP3:                                                                                                                  
    SP4:                                                                                                                  

    About this scheme

    ECgene alternative splicing isoforms for UBA2

    1 Ensembl transcript including schematic representation:
    ENST00000246548  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    UBA2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for UBA2

    1 / 2

    3 probe-sets matching UBA2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    34814_at2 U95-A 2 1.00 0.59 1.00 1.00 -- -- -- -- --

    201177_s_at2 U133-A 2 1.00 0.55 -- -- -- -- -- -- --

    201177_s_at2 U133Plus2 2 1.00 0.55 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TTGCCTTTTT

    SOURCE GeneReport for Unigene cluster: Hs.631580

    Expression variation in blood from EXPOLDB for UBA2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for UBA2 gene from 5/24 species (see all 24 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    UBA21   -- ubiquitin-like modifier activating enzyme 2 94.12(n)
    97.79(a)
    476490  XM_533699.2  XP_533699.2 
    chimpanzee
    (Pan troglodytes)
    UBA21   -- ubiquitin-like modifier activating enzyme 2 99.79(n)
    100(a)
    468823  XM_001155735.1  XP_001155735.1 
    cow
    (Bos taurus)
    UBA21   -- ubiquitin-like modifier activating enzyme 2 93.65(n)
    97.97(a)
    507005  XM_583543.3  XP_583543.3 
    rat
    (Rattus norvegicus)
    Uba21   -- ubiquitin-like modifier activating enzyme 2 85.2(n)
    91.25(a)
    308508  XM_218502.4  XP_218502.4 
    mouse
    (Mus musculus)
    Uba21, 5 75
    ubiquitin-like modifier activating enzyme 21, 5 87.72(n)1
    94.36(a)1
    509951  NM_016682.21  NP_057891.11 
     AA9860915  AK0421705  (see all 59)
    About this table        Species with no ortholog for UBA2

    ENSEMBL Gene Tree for UBA2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for UBA2 gene
    UBA32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/195 NCBI SNPs in UBA2 are shown (see all 195 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 51)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 19 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs10430701,2
    A,C,F39652248(+) TACCCT/GTAGGT 1 -- ut31 ese31Minor allele frequency- G:0.09MN 184
    rs22878301,2
    F39611139(-) GGCGGG/TAGAAC 1 -- ut51 ese31Minor allele frequency- T:0.03EA 1486
    rs10430621,2
    H39634774(+) AGGCCT/GGAAAG 1 R/L mis1 ese38Minor allele frequency- G:0.00NA EA MN EU WA 840
    rs72591601,2
    A,C,F,H39651819(+) GATTCG/AGATGA 1 S/S syn18Minor allele frequency- A:0.16NA EU EA WA 618
    rs15591961,2
    A,C,F,H,O39643059(-) AAGCCA/GTGGGG 1 -- int111Minor allele frequency- G:0.32MN EU EA WA NA 1000
    rs10302161,2
    A,C,F,H,O39650771(-) TCATAC/TTGGTG 1 -- int110Minor allele frequency- N:0.00NA EA EU 755
    rs116733311,2
    A,C,F,H39644343(+) GGAATC/TTCAGT 1 -- int111Minor allele frequency- T:0.34NA EU EA WA 976
    --
    rs48050801,2
    A,C,F,H39623744(+) cgtgtT/Ctattc 1 -- int14Minor allele frequency- C:0.20EU EA WA 418
    rs28639821,2
    C,F,H,O39619372(-) GAAGCT/CGTGTT 1 -- int14Minor allele frequency- C:0.15EU EA WA 412
    rs37870341,2
    A,C,F,H39631370(+) GGCAGT/ATGTGT 1 -- int14Minor allele frequency- A:0.34EU EA WA 414
    About this table

    HapMap Linkage Disequilibrium images for UBA2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/24 PubMed articles for UBA2 gene (see all 24 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10054 HGNC: 30661 AceView: UBA2 Ensembl:ENSG00000126261 euGenes: HUgn10054
    ECgene: UBA2 H-InvDB: UBA2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for UBA2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for UBA2-related products

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     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for UBA2 Antibodies for UBA2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for UBA2 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
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     Antibodies (AOS1+UBA2, SAE2 / UBA2)
     Search OriGene for UBA2
     Search Tocris compounds for UBA2




     Assays/Proteins for UBA2
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for UBA2

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