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U2AF1L4 Gene

protein-coding   GIFtS: 46

GC19M040926
U2 small nuclear RNA auxiliary factor 1-like 4
(Previous names: U2(RNU2) small nuclear RNA auxiliary factor 1-like 3, U2 small nuclear RNA auxiliary factor 1-like 3 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: U2AF1L3)
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Aliases &
Descriptions
for U2AF1L4

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
FLJ35525 2
MGC33901 1, 2
U2AF1-RS3 2, 3
U2AF1L3 2, 3
U2AF1L3V1 2
U2AF1RS3 2
U2af26 1, 2
Descriptions
U2 auxiliary factor 26 3
U2 small nuclear RNA auxiliary factor 1-like 3 1, 2
U2 small nuclear RNA auxiliary factor 1-like 4 2
U2 small nuclear RNA auxiliary factor 1-like protein 3 3
U2 small nuclear RNA auxiliary factor 1-like protein 4 3
U2(RNU2) small nuclear RNA auxiliary factor 1-like 3 1, 2
U2(RNU2) small nuclear RNA auxiliary factor 1-like protein
3 3
U2AF1-like protein 3 3
External Ids
HGNC: 230201
Entrez Gene: 1997462
UniProtKB: Q8WU683
Ensembl: ENSG000001612657
Search outside databases for aliases for U2AF1L4 gene

Previous GC identifer: GC00U914419

Summaries
for U2AF1L4

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: U2AF4_HUMAN, Q8WU68
Function: RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in
both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and
protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the
binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative
pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed
by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site (By similarity)

Genomic Location
for U2AF1L4

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the U2AF1L4 gene  

Entrez Gene cytogenetic band: 19q13.12   Ensembl cytogenetic band:  19q13.12   HGNC cytogenetic band: 19q13.13

U2AF1L4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M040926:     (about GC identifiers)

Start:
 40,925,268 bp from pter
End:
 40,928,176 bp from pter
Size:
 2,909 bases
Orientation:
 minus strand
RefSeq DNA sequence:
NC_000019.8  NT_011109.15  

Proteins
for U2AF1L4

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: U2AF4_HUMAN, Q8WU68 (See protein sequence)
Recommended Name: Splicing factor U2AF 26 kDa subunit  
Size: 220 amino acids; 25744 Da
Subunit: Interacts with GFI1 and U2AF2 (By similarity)
Subcellular location: Nucleus (By similarity). Nucleus speckle (By similarity)
Secondary accessions: A6NKI8 Q56UU3
Alternative splicing: 3 isoforms:  Q8WU68-1   Q8WU68-2   Q8WU68-3   

REFSEQ proteins (2 alternative transcripts): 
NP_001035515.1  NP_659424.2  


ENSEMBL proteins: 
ENSP00000292879 ENSP00000376034 ENSP00000368258 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (U2AF1L3)
Human Recombinant Proteins from Abnova (U2AF1L3)
              OriGene Purified Recombinant Human Protein: U2AF1L4 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
GO:0005681 spliceosome IEA--
GO:0016607 nuclear speck IEA--
About this table

Antibodies for U2AF1L4: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for U2AF1L4
Browse R&D Systems for Antibodies
Antibodies from Abcam (U2AF1L3), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (U2AF1L3)
Novus Biologicals Antibodies for U2AF1L4

Assays for U2AF1L4: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

Protein Domains/
Families
for U2AF1L4

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
5 InterPro domains/families:
 IPR000571 Znf_CCCH
 IPR012677 a_b_plait_nuc_bd
 IPR003954 RRM_1
 IPR000504 RRM_RNP1
 IPR009145 U2_small


   GeneDecks  U2AF1L4 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q8WU68

ProtoNet protein and cluster: Q8WU68

2 Blocks protein families:
IPB000504 RNA-binding region RNP-1 (RNA recognition motif)
IPB000571 Zn-finger


UniProtKB/Swiss-Prot: U2AF4_HUMAN, Q8WU68
Domain: The second zinc finger in necessary for interaction with GFI1 and for alternative pre-mRNA
splicing events (By similarity)
Similarity: Belongs to the splicing factor SR family
Similarity: Contains 2 C3H1-type zinc fingers
Similarity: Contains 1 RRM (RNA recognition motif) domain

Gene Function
for U2AF1L4

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (U2AF1L4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (U2AF1L3)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_144987

              Applied Biosystems Silencer® siRNAs for U2AF1L4

              Sigma-Aldrich siRNA for U2AF1L4  
                     Sigma-Aldrich shRNA for U2AF1L4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for U2AF1L4
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001040425
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001040425
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001040425 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_144987

UniProtKB/Swiss-Prot: U2AF4_HUMAN, Q8WU68
Function: RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in
both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and
protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the
binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative
pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed
by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site (By similarity)

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003723 RNA binding IEA--
GO:0008270 zinc ion binding IEA--
GO:0046872 metal ion binding IEA--
About this table

Pathways & Interactions
for U2AF1L4

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006397 mRNA processing IEA--
GO:0008380 RNA splicing IEA--
About this table

Drugs & Compounds
for U2AF1L4

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for U2AF1L4

Transcripts
for U2AF1L4

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section
Inhib.
RNA:		Invitrogen RNAi Products for gene knock-down (U2AF1L4)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (U2AF1L3)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_144987

              Sigma-Aldrich siRNA for U2AF1L4  
                     Sigma-Aldrich shRNA for U2AF1L4  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001040425  NM_144987  

REFSEQ mRNAs for U2AF1L4 gene (2 alternative transcripts): 

NM_001040425.1   NM_144987.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001040425  NM_144987  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001040425
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001040425
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001040425 

Additional cDNA sequence: 

AK092844.1 AK303983.1 AY461582.1 AY569437.3 BC010865.1 BC021186.1 BC049208.1 BC062474.1 

24 DOTS entries:

DT.101984198  DT.100789061  DT.101984199  DT.213499  DT.121488876  DT.121488788  DT.213497  DT.100700046 
DT.100789057  DT.99936823  DT.100699338  DT.95161758  DT.91660602  DT.100789056  DT.121488888  DT.121488838 
DT.121488905  DT.422019  DT.100037322  DT.100789054  DT.100789055  DT.100789060  DT.121488843  DT.95188629 

24/175 AceView cDNA sequences (see all 175 ):

AW672936 AI970395 NM_024660 BQ010545 AI597639 BX337984 AI921582 AW978267 
BC010865 BU735432 AK026226 CA748721 CD367166 BC031329 BE910002 BI838452 
AI925610 BQ882427 AI311085 BC049208 BU608847 BF445882 BU621457 AI719948 

highest scoring ESTs for U2AF1L4:

BC010865 BC021186 BC049208 BI548776 CB068531 CD175846 AA503778 AY461582 CN414589 CV024513 

Unigene Cluster for U2AF1L4:

U2 small nuclear RNA auxiliary factor 1-like 4
Hs.351558  [show with all ESTs]
Unigene Representative Sequence: BC049208


GeneLoc Exon Structure

5/15 Alternative Splicing Database (ASD) splice patterns (SP) for U2AF1L4 (see all 15 )

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c ^ 8a · 8b · 8c · 8d ^ 9 ^ 10 ^ 11a · 11b ·
SP1:                                -                 -     -     -     -     -                             -                       -     -     -               
SP2:                                -     -           -     -     -     -     -                             -                       -     -     -     -         
SP3:                                -                                                                       -                       -     -     -               
SP4:                                -                 -     -     -     -     -                                                     -     -     -               
SP5:                                -     -           -                                                     -                                                   

ExUns: 11c · 11d · 11e
SP1:                  
SP2:                  
SP3:                  
SP4:                  
SP5:                  

About this scheme

ECgene alternative splicing isoforms for U2AF1L4

3 Ensembl transcripts including schematic representations:
ENST00000292879  ENST00000392196  ENST00000378975  

Expression
for U2AF1L4

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

U2AF1L4 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for U2AF1L4

1 / 2 / 3

5 probe-sets matching U2AF1L4 gene


Affymetrix
probe-set		 Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

57187_at2, 3 U95-B 1 1.00 1.00 0.73 1.09 AI688411 0.60 0.83 0.73 1
45569_at2, 3 U95-B 1 1.00 1.00 0.94 0.83 AW014435 0.60 0.83 0.73 1

57188_g_at2, 3 U95-B 1 1.00 1.00 0.90 1.08 AI688411 0.60 0.83 0.73 1

226700_at2, 3 U133-B 1 1.00 1.00 -- -- BE856544 0.40 0.75 0.60 1

226700_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  U2AF1L4 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GAGACCTTCT

SOURCE GeneReport for Unigene cluster: Hs.351558

UniProtKB/Swiss-Prot: U2AF4_HUMAN, Q8WU68
Tissue specificity: Isoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain
and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes

Orthologs
for U2AF1L4

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section
Orthologs for U2AF1L4 gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity		 NCBI accessions
dog
(Canis familiaris)		 TMEM1491   -- transmembrane protein 149 92.08(n)
93.92(a)		 476480  XM_848596.1  XP_853689.1 
chimpanzee
(Pan troglodytes)		 TMEM1491   -- transmembrane protein 149 98.71(n)
97.79(a)		 455961  XM_001160796.1  XP_001160796.1 
cow
(Bos taurus)		 TMEM1491   -- transmembrane protein 149 90.24(n)
92.82(a)		 615198  NM_001034778.1  NP_001029950.1 
rat
(Rattus norvegicus)		 U2af1l41   -- U2 small nuclear RNA auxiliary factor 1-like 4 90.06(n)
93.37(a)		 361542  NM_001008775.1  NP_001008775.1 
mouse
(Mus musculus)		 U2af1l41, 5 75
 U2 small nuclear RNA auxiliary factor 1-like 41, 5 89.5(n)1
93.37(a)1		 2330731  NM_170760.31  NP_739566.11 
 AF4193395  AF4193405  (see all 18)
About this table        Species with no ortholog for U2AF1L4

ENSEMBL Gene Tree for U2AF1L4

Paralogs
for U2AF1L4

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for U2AF1L4 gene
ZRSR22  U2AF12  ZRSR12  

SNPs/Variants
for U2AF1L4

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

  --

Disorders & Mutations
for U2AF1L4

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

 OMIM: 601080

Medical News
for U2AF1L4

(Possibly Related Articles in Doctor's Guide)
About This Section

   --

Publications
for U2AF1L4

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/11 PubMed articles for U2AF1L4 gene (see all 11 ):

Search for U2AF1L4

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  
  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

Genome Databases showing U2AF1L4

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section
Entrez Gene: 199746 HGNC: 23020 AceView: FLJ22573andU2AF1L3 Ensembl:ENSG00000161265 euGenes: HUgn199746
ECgene: U2AF1L4 H-InvDB: U2AF1L4

Other Databases showing U2AF1L4

(According to HUGE)
About This Section

   --

Specialized Databases showing U2AF1L4

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

   --

Licensable Technologies
for U2AF1L4

(Available from WIS Yeda, Salk, Tufts)
About This Section

  --

Services
for U2AF1L4

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for U2AF1L4:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Recombinant Proteins
(U2AF1L3)
 Antibodies (U2AF1L3)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for U2AF1L4




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for U2AF1L4

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