Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms of Use
- Terms of Use
- 3rd Party Notice
About Us
- GeneCards Team
- Our Publications
- Citing GeneCards
- Licensing Agreement
- Weizmann Institute
- LifeMap Sciences Inc
User Feedback
GeneCards Plus
- Premium Tools
  
  Learn more

Set Analyses:

VarElect

Advanced Search

TYW1 Gene

protein-coding GIFtS: 53
GCID: GC07P066460

TRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae)

(Previous name: radical S-adenosyl methionine and flavodoxin domains 1)
(Previous symbol: RSAFD1)

See related disease
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

TryGeneCards Plus

Aliases
TRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae)¹ ²		TYW1A²
RSAFD1¹ ² ³		YPL207W²
Radical S-Adenosyl Methionine And Flavodoxin Domains 1¹ ²		S-Adenosyl-L-Methionine-Dependent TRNA 4-Demethylwyosine Synthase²
Radical S-Adenosyl Methionine And Flavodoxin Domain-Containing Protein 1² ³		TRNA-YW Synthesizing Protein 1 Homolog A²
TRNA Wybutosine-Synthesizing Protein 1 Homolog² ³		EC 4.1.3.44³
TRNA-YW Synthesizing Protein 1 Homolog A (S. Cerevisiae)¹		TRNA-YW-Synthesizing Protein³

External Ids:	HGNC: 25598¹	Entrez Gene: 55253²	Ensembl: ENSG00000198874⁷	OMIM: 611243⁵	UniProtKB: Q9NV66³

Export aliases for TYW1 gene to outside databases

Previous GC identifers: GC07P066099 GC07P062645

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for TYW1 Gene:

Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes

codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis

of wybutosine. (provided by RefSeq, Dec 2012)

GeneCards Summary for TYW1 Gene:

TYW1 (tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)) is a protein-coding gene. Diseases associated with TYW1 include fanconi's anemia. GO annotations related to this gene include 4 iron, 4 sulfur cluster binding and iron ion binding. An important paralog of this gene is TYW1B.

UniProtKB/Swiss-Prot: TYW1_HUMAN, Q9NV66

Function: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with

a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes

the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an

intermediate in wybutosine biosynthesis (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus

RefSeq DNA sequence:

NC_000007.14 NT_007933.16 NC_018918.2

Regulatory elements:

Regulatory transcription factor binding sites in the TYW1 gene promoter:
SRF Elk-1 SRF (504 AA) RREB-1 FOXO3 FOXO3b GATA-1 RORalpha1 STAT3 FOXO3a
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): TYW1 promoter sequence

Search Chromatin IP Primers for TYW1

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TYW1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q11.21 Ensembl cytogenetic band: 7q11.21 HGNC cytogenetic band: 7q11.21

TYW1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TYW1 gene location

GeneLoc information about chromosome 7 GeneLoc Exon Structure

GeneLoc location for GC07P066460: view genomic region (about GC identifiers)

Start:	66,460,160 bp from pter	End:	66,704,501 bp from pter
Size:	244,342 bases	Orientation:	plus strand

1 alternative location:

Chr7+,CRA_TCAG 65,799,379-66,042,061

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: TYW1_HUMAN, Q9NV66 (See protein sequence)

Recommended Name: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Size: 732 amino acids; 83702 Da

Cofactor: Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable

S-adenosyl-L-methionine (By similarity)

Sequence caution: Sequence=AAH15591.1; Type=Frameshift; Positions=663; Sequence=BAB14307.1; Type=Erroneous

initiation;

Secondary accessions: Q6PJG8 Q75MG8 Q75MN3 Q86V12 Q8IVS7 Q9H9C4

Alternative splicing: 2 isoforms: Q9NV66-1 Q9NV66-2 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for TYW1: NX_Q9NV66

Explore proteomics data for TYW1 at MOPED

Post-translational modifications:

Modification sites at neXtProt

Modification sites at PhosphoSitePlus

See TYW1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_060734.2

ENSEMBL proteins:

ENSP00000352645 ENSP00000398897 ENSP00000354795

TYW1 Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate for TYW1 OriGene Custom MassSpec OriGene Custom Protein Services for TYW1
	GenScript Custom Purified and Recombinant Proteins Services for TYW1
	Novus Biologicals TYW1 Proteins Novus Biologicals TYW1 Lysates
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Cloud-Clone Corp. Proteins for TYW1

TYW1 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	OriGene Antibodies for TYW1 OriGene Custom Antibody Services for TYW1
	Novus Biologicals TYW1 Antibodies
	Abcam antibodies for TYW1
	Cloud-Clone Corp. Antibodies for TYW1
	ThermoFisher Antibodies for TYW1
	LSBio Antibodies in human, mouse, rat for TYW1

TYW1 Assay Products:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for TYW1
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for TYW1
	Browse Enzo Life Sciences for kits & assays
	Cloud-Clone Corp. ELISAs for TYW1 Cloud-Clone Corp. CLIAs for TYW1

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TryGeneCards Plus

5 InterPro protein domains:

IPR013785 Aldolase_TIM

IPR001094 Flavdoxin

IPR008254 Flavodoxin/NO_synth

IPR007197 rSAM

IPR013917 tRNA_wybutosine-synth

Graphical View of Domain Structure for InterPro Entry Q9NV66

ProtoNet protein and cluster: Q9NV66

3 Blocks protein domains:

IPB001094 Flavodoxin signature

IPB008254 Flavodoxin/nitric oxide synthase

IPB013917 Wyosine base formation

UniProtKB/Swiss-Prot: TYW1_HUMAN, Q9NV66

Similarity: Belongs to the TYW1 family

Similarity: Contains 1 flavodoxin-like domain

TYW1 for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
About This Section

TryGeneCards Plus

Molecular Function:

UniProtKB/Swiss-Prot Summary: TYW1_HUMAN, Q9NV66

Function: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with

a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes

the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an

intermediate in wybutosine biosynthesis (By similarity)

Catalytic activity: N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine =

4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO(2) + H(2)O

Enzyme Number (IUBMB): EC 4.1.3.44¹

Gene Ontology (GO): Selected molecular function terms (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003824	catalytic activity	--	--
GO:0005506	iron ion binding	IEA	--
GO:0010181	FMN binding	IEA	--
GO:0016491	oxidoreductase activity	IEA	--
GO:0016829	lyase activity	IEA	--

TYW1 for ontologies About GeneDecksing

Animal Models:

inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for TYW1

inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for TYW1

genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for TYW1

genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for TYW1

miRNA Products:		miRTarBase miRNAs that target TYW1: hsa-mir-26a-5p (MIRT050089)

		Block miRNA regulation of human, mouse, rat TYW1 using miScript Target Protectors
		4 qRT-PCR Assays for microRNAs that regulate TYW1:
		hsa-miR-549 hsa-miR-148b* hsa-miR-2355-3p hsa-miR-3672
		SwitchGear 3'UTR luciferase reporter plasmid: TYW1 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for TYW1
		Predesigned siRNA for gene silencing in human, mouse, rat TYW1

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for TYW1

Clone Products:		OriGene clones in human, mouse for TYW1 (see all 6) OriGene ORF clones in mouse, rat for TYW1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TYW1 (NM_018264)
		Sino Biological Human cDNA Clone for TYW1
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TYW1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TYW1

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for TYW1
		Browse ESI BIO Cell Lines and PureStem Progenitors for TYW1

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TYW1

(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
About This Section

TryGeneCards Plus

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
cytosol	2
endoplasmic reticulum	1
extracellular	1
golgi apparatus	1
nucleus	1
peroxisome	1
plasma membrane	1

About this image

Gene Ontology (GO): 3 cellular component terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	--

TYW1 for ontologies About GeneDecksing

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
About This Section

TryGeneCards Plus

SuperPaths for TYW1 About

See pathways by source

SuperPath

Contained pathways About

wybutosine biosynthesis

7-(3-amino-3-carboxypropyl)-wyosine biosynthesis

0.00

wybutosine biosynthesis

2 BioSystems Pathways for TYW1

	wybutosine biosynthesis
	7-(3-amino-3-carboxypropyl)-wyosine biosynthesis

UniProtKB/Swiss-Prot: TYW1_HUMAN, Q9NV66

Pathway: tRNA modification; wybutosine-tRNA(Phe) biosynthesis

Custom Pathway & Disease-focused RT² Profiler PCR Arrays for TYW1
Interactions:

Search GeneGlobe Interaction Network for TYW1

STRING Interaction Network Preview (showing 5 interactants - click image to see 15)

Selected Interacting proteins for TYW1 (Q9NV66³ ENSP00000352645⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 39)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
GRWD1	Q9BQ67³, ENSP00000253237⁴	I2D: score=2 STRING: ENSP00000253237
EIF5A	P63241³, ENSP00000336702⁴	I2D: score=1 STRING: ENSP00000336702
UBC	P0CG48³, ENSP00000344818⁴	I2D: score=2 STRING: ENSP00000344818
PWP1	Q13610³	I2D: score=4
HSP90AA1	P07900³	I2D: score=3

About this table

Gene Ontology (GO): 1 biological process term: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008033	tRNA processing	IEA	--

TYW1 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
About This Section

TryGeneCards Plus

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse compounds at ApexBio

Browse Tocris compounds for TYW1

2 HMDB Compounds for TYW1 About this table

Compound	Synonyms	CAS #	PubMed Ids
Flavin Mononucleotide	FMN (see all 19)	146-17-8	--
S-Adenosylmethionine	(3S)-5'-[(3-amino-3-carboxypropyl)methylsulfonio]-5'-deoxyadenosine (see all 16)	29908-03-0	--

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
About This Section

TryGeneCards Plus

REFSEQ mRNAs for TYW1 gene:

NM_018264.3

Unigene Cluster for TYW1:

TRNA-yW synthesizing protein 1 homolog (S. cerevisiae)

Hs.520917 [show with all ESTs]

Unigene Representative Sequence: NM_018264

6 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000491969 ENST00000359626(uc003tvn.3 uc010lai.3) ENST00000442959

ENST00000475392 ENST00000361660 ENST00000495971

miRNA Products:		Block miRNA regulation of human, mouse, rat TYW1 using miScript Target Protectors
		4 qRT-PCR Assays for microRNAs that regulate TYW1:
		hsa-miR-549 hsa-miR-148b* hsa-miR-2355-3p hsa-miR-3672
		SwitchGear 3'UTR luciferase reporter plasmid: TYW1 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for TYW1
		Predesigned siRNA for gene silencing in human, mouse, rat TYW1

Clone Products:		OriGene clones in human, mouse for TYW1 (see all 6) OriGene ORF clones in mouse, rat for TYW1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TYW1 (NM_018264)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TYW1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TYW1

Primer Products:		OriGene qPCR primer pairs and template standards for TYW1 OriGene qSTAR qPCR primer pairs in human, mouse for TYW1
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat TYW1
		QuantiTect SYBR Green Assays in human, mouse, rat TYW1
		QuantiFast Probe-based Assays in human, mouse, rat TYW1

Additional mRNA sequence:

AK001762.1 AK022917.1 AK301330.1 BC015591.2 BC042156.1 BC051888.1

12 DOTS entries:

DT.100789029 DT.99933881 DT.316409 DT.100774174 DT.423111 DT.105466 DT.92435437 DT.95370849

DT.100776166 DT.100789030 DT.95256568 DT.100674468

Selected AceView cDNA sequences (see all 167):

AA114967 AI073929 BM768300 AI745344 BU633885 AA115497 BC051888 NM_018264

BU628109 AA115040 BC042156 CB306182 BF434912 CD369837 BQ221024 AA953059

AI273336 BX112421 AA653716 AU122057 AI277061 BM989121 AA115025 AA582390

GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for TYW1 About this scheme

ExUns:	1	^	2	^	3	^	4	^	5	^	6	^	7	^	8	^	9	^	10a	·	10b	^	11	^	12	^	13a	·	13b
SP1:
SP2:
SP3:
SP4:																									-

ECgene alternative splicing isoforms for TYW1

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

TryGeneCards Plus

TYW1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: --

About this image

TYW1 Protein expression data from MOPED¹, PaxDb² and MaxQB³:

About this image

TYW1 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.520917

Custom PCR Arrays for TYW1

Primer Products:		OriGene qPCR primer pairs and template standards for TYW1 OriGene qSTAR qPCR primer pairs in human, mouse for TYW1
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat TYW1
		QuantiTect SYBR Green Assays in human, mouse, rat TYW1
		QuantiFast Probe-based Assays in human, mouse, rat TYW1

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TYW1

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

TryGeneCards Plus

This gene was present in the common ancestor of eukaryotes.

Orthologs for TYW1 gene from Selected species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Details
mouse (Mus musculus)	Mammalia	Tyw1^{1 , 5}	tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) less^{1, 5}	80.75(n)¹ 81.87(a)¹	5 (69.00 cM)⁵ 100929¹ NM_001015876.2¹ NP_001015876.1¹ 130255619⁵
chicken (Gallus gallus)	Aves	TYW1¹	tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) less	76.65(n) 83.79(a)	417478 XM_003642390.2 XP_003642438.1
African clawed frog (Xenopus laevis)	Amphibia	CA788486.1²	--	78.51(n)	CA788486.1
zebrafish (Danio rerio)	Actinopterygii	tyw1¹	tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) less	68.26(n) 71.07(a)	562076 NM_001077156.1 NP_001070624.1
baker's yeast (Saccharomyces cerevisiae)	Saccharomycetes	TYW1(YPL207W)⁴ TYW1¹	Protein required for the synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions less⁴ TYW1¹	50.53(n)¹ 46.89(a)¹	16(159909-162341)⁴ 855894^{1, 4} NP_015117.1^{1, 4}
thale cress (Arabidopsis thaliana)	eudicotyledons	AT1G75200¹	AT1G75200	56.09(n) 54.2(a)	843857 NM_106176.5 NP_177656.2
rice (Oryza sativa)	Liliopsida	Os.33461²	Oryza sativa (japonica cultivar-group) cDNA cloneJ023099L20, full insert sequence less	76.77(n)	AK100507.1

ENSEMBL Gene Tree for TYW1 (if available)
TreeFam Gene Tree for TYW1 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

TryGeneCards Plus

Paralogs for TYW1 gene

TYW1B²

1 SIMAP similar gene for TYW1 using alignment to 2 protein entries: TYW1_HUMAN (see all proteins):

TYW1B

TYW1 for paralogs About GeneDecksing

2 Pseudogenes.org Pseudogenes for TYW1
PGOHUM00000250454 PGOHUM00000250873

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
About This Section

TryGeneCards Plus

SNP ID	Valid	Clinical significance	Chr 7 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs4718439^1,2	C,F,A,H	--	65800229(+)	`CTGGAG/ATGCGG`	1	--	int¹		8		NS EA NA WA	412
rs4718440^1,2	C,F,A,H	--	65800241(+)	`GGCGCA/GATCTT`	1	--	int¹		4		NA WA	8
rs146670995^1,2		--	65800338(+)	`ACGCCC/TGGCTA`	1	--	int¹		0	--	--	--	--
rs11770114^1,2	C,H	--	65800350(+)	`tttttA/Gtattt`	1	--	int¹		0	--	--	--	--
rs186274509^1,2		--	65800443(+)	`AAAGTA/GCTGGA`	1	--	int¹		0	--	--	--	--
rs192510151^1,2		--	65800475(+)	`GCCCGG/TCCAAC`	1	--	int¹		0	--	--	--	--
rs114797515^1,2	F	--	65800556(+)	`TTGGGA/GTGGAT`	1	--	int¹		1		WA	118
rs138394993^1,2	F	--	65800630(+)	`GATACA/GTGGGA`	2	T	syn¹		1		NA	4542
rs201637088^1,2		--	65800732(+)	`ATTGTA/CATCAA`	2	V	syn¹		0	--	--	--	--
rs147995512^1,2	C,F	--	65800740(+)	`CAAGAC/TGCAGG`	2	T M	mis¹		2		NA EU	5873

HapMap Linkage Disequilibrium report for TYW1 (66460160 - 66704501 bp)

Structural Variations
Database of Genomic Variants (DGV) Selected variations for TYW1 (see all 18): About this table

Variant ID	Type	Subtype	PubMed ID
esv2253832	CNV	Deletion	18987734
esv2734604	CNV	Deletion	23290073
esv2467105	CNV	Deletion	19546169
esv2422035	CNV	Deletion	20811451
esv2666250	CNV	Deletion	23128226
esv2660507	CNV	Deletion	23128226
nsv509205	CNV	Insertion	20534489
nsv5774	CNV	Insertion	18451855
nsv824151	CNV	Loss	20364138
esv33653	CNV	Loss	17666407

Site Specific Mutation Identification with PCR Assays

SeqTarget long-range PCR primers for resequencing TYW1

DNA2.0 Custom Variant and Variant Library Synthesis for TYW1

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

TryGeneCards Plus

OMIM gene information: 611243 OMIM disorders: --

1 disease for TYW1:
About MalaCards

fanconi's anemia

TYW1 for disorders About GeneDecksing

Genetic Association Database (GAD): TYW1
Human Genome Epidemiology (HuGE) Navigator: TYW1 (1 document)

Export disorders for TYW1 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

TryGeneCards Plus

PubMed articles for TYW1 gene, integrated from 10 sources (see all 14):
(articles sorted by number of sources associating them with TYW1)

Utopia: connect your pdf to the dynamic
world of online information

Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)^{1, 4} Rose J.E....Uhl G.R. (Mol. Med. 2010)
Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. (PubMed id 17150819)^{1, 3} Noma A. and Suzuki T. (Nucleic Acids Symp Ser (Oxf) 2006)
Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs. (PubMed id 16162496)^{1, 3} Waas W.F....Schimmel P. (J. Biol. Chem. 2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S.... Malek J. (Genome Res. 2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (Nat. Genet. 2004)
The DNA sequence of human chromosome 7. (PubMed id 12853948)^{1, 2} Hillier L.W.... Wilson R.K. (Nature 2003)
Protein interaction discovery using parallel analysis of translated ORFs (PLATO). (PubMed id 23503679)¹ Zhu J....Elledge S.J. (Nat. Biotechnol. 2013)
Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)¹ Udeshi N.D....Carr S.A. (amp 2012)
MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity. (PubMed id 22678362)¹ Stehling O.... Lill R. (Science 2012)
Systematic and quantitative assessment of the ubiquitin-modified proteome. (PubMed id 21906983)¹ Kim W....Gygi S.P. (Mol. Cell 2011)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

TryGeneCards Plus

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

TryGeneCards Plus

Entrez Gene: 55253	HGNC: 25598	AceView: FLJ10900	Ensembl:ENSG00000198874	euGenes: HUgn55253
ECgene: TYW1	H-InvDB: TYW1

(According to HUGE)
About This Section

TryGeneCards Plus

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
About This Section

TryGeneCards Plus

Name	Description
PharmGKB entry for TYW1	Pharmacogenomics, SNPs, Pathways

Intellectual Property for TYW1 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

TryGeneCards Plus

Patent Information for TYW1 gene:
Search GeneIP for patents involving TYW1

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
About This Section

TryGeneCards Plus


	EMD Millipore genomic analysis products

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Enzyme Activity Assays/Reagents
	Browse ELISpot/FluoroSpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Luminex Assays
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Recombinant/Natural Proteins		Browse Stem Cell Products
	Browse cDNA Clones		Browse Proteome Profiler Antibody Arrays

	OriGene Antibodies for TYW1		OriGene RNAi products in human, mouse, rat for TYW1
	OriGene qPCR primer pairs and template standards for TYW1		OriGene Protein Over-expression Lysate for TYW1
	OriGene Custom Mass Spec		OriGene clones in human, mouse for TYW1
	OriGene qSTAR qPCR primer pairs in human, mouse for TYW1		Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
	OriGene ORF clones in mouse, rat for TYW1		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for TYW1		OriGene Custom Protein Services for TYW1

	Block miRNA regulation of human, mouse, rat TYW1 using miScript Target Protectors		SeqTarget long-range PCR primers for resequencing TYW1
	DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TYW1		Predesigned siRNA for gene silencing in human, mouse, rat TYW1
	QuantiFast Probe-based Assays in human, mouse, rat TYW1		QuantiTect SYBR Green Assays in human, mouse, rat TYW1
	Custom PCR Arrays for TYW1		Search Chromatin IP Primers for TYW1
	Pre-validated RT² qPCR Primer Assay in human, mouse, rat TYW1		Search GeneGlobe Interaction Network for TYW1
	Regulatory tfbs in TYW1 promoter

	GenScript Custom Purified and Recombinant Proteins Services for TYW1		GenScript cDNA clones with any tag delivered in your preferred vector for TYW1
	GenScript Custom Assay Services for TYW1		GenScript Custom overexpressing Cell Line Services for TYW1
	CloneReady with Over 120,000 Genes		Gene Synthesis: Any Gene in Any Vector
	Vector-based siRNA and miRNA, Ready for Transfection		Gene Mutant Library, Variants up to 10^11
	Plasmid Preparation		Custom Peptide Services

Search for Antibodies & Assays

Search Tocris compounds for TYW1

	Browse Sino Biological Proteins
	Browse Sino Biological Cell Lysates
	cDNA Clones for TYW1
	4000+ Proteins
	Search Sino Biological for antibodies, proteins & pathways
	Protein Production Services
	Transfection Reagents
	Protein A/G/L resins
	Isotyping reagents

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	TYW1 antibodies
	TYW1 proteins
	TYW1 lysates

	Antibodies for TYW1
	See all of Abcam's Antibodies, Kits and Proteins for TYW1
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

	Proteins for TYW1
	Antibodies for TYW1
	ELISAs for TYW1
	CLIAs for TYW1

Browse ESI BIO Cell Lines and PureStem Progenitors for TYW1

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TYW1

	SwitchGear 3'UTR luciferase reporter plasmids for TYW1
	SwitchGear Promoter luciferase reporter plasmids for TYW1

ThermoFisher Antibodies for TYW1

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TYW1

	inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for TYW1
	inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for TYW1

LSBio Antibodies in human, mouse, rat for TYW1

Customized transgenic rodents for:

	Humanization
	Biomarker expression
	Off-target effect monitoring
	Translational medicine
	Tissue-specific gene expresssion
	Time-controlled gene expresssion

Browse compounds at ApexBio

GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

View Random Gene

Category			(GIFtS: )
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes TYW1 gene at Home site.

Version: 3.12.142 28 July 2014
hostname: 356980-web2.xennexinc.com index build: 126 solr: 1.4

GeneCards Plus

Advanced Search

Export list of displayed genes

TYW1 Gene

TRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae)

Aliases
for TYW1 gene

Summaries
for TYW1 gene

Genomic Views
for TYW1 gene

Proteins
for TYW1 gene

TYW1 Human Recombinant Protein Products:

TYW1 Antibody Products:

TYW1 Assay Products:

Domains /
Families
for TYW1 gene

Function
for TYW1 gene

Localization
for TYW1 gene

Pathways & Interactions
for TYW1 gene

Drugs & Compounds
for TYW1 gene

Transcripts
for TYW1 gene

Expression
for TYW1 gene

Orthologs
for TYW1 gene

Paralogs
for TYW1 gene

Genomic Variants
for TYW1 gene

Disorders / Diseases
for TYW1 gene

Publications
for TYW1 gene

External Searches for TYW1 gene

Genome Databases showing TYW1 gene

Other Databases showing TYW1 gene

Specialized Databases showing TYW1 gene

Intellectual Property
for TYW1 gene

Products
for TYW1 gene

View Random Gene

GeneCards Plus

Advanced Search

Export list of displayed genes

TYW1 Gene

TRNA-YW Synthesizing Protein 1 Homolog (S. Cerevisiae)

Aliasesfor TYW1 gene

Summariesfor TYW1 gene

Genomic Viewsfor TYW1 gene

Proteinsfor TYW1 gene

TYW1 Human Recombinant Protein Products:

TYW1 Antibody Products:

TYW1 Assay Products:

Domains /Familiesfor TYW1 gene

Functionfor TYW1 gene

Localizationfor TYW1 gene

Pathways & Interactionsfor TYW1 gene

Drugs & Compoundsfor TYW1 gene

Transcriptsfor TYW1 gene

Expression for TYW1 gene

Orthologsfor TYW1 gene

Paralogsfor TYW1 gene

Genomic Variantsfor TYW1 gene

Disorders / Diseasesfor TYW1 gene

Publicationsfor TYW1 gene

External Searches for TYW1 gene

Genome Databases showing TYW1 gene

Other Databases showing TYW1 gene

Specialized Databases showing TYW1 gene

Intellectual Propertyfor TYW1 gene

Productsfor TYW1 gene

View Random Gene

Aliases
for TYW1 gene

Summaries
for TYW1 gene

Genomic Views
for TYW1 gene

Proteins
for TYW1 gene

Domains /
Families
for TYW1 gene

Function
for TYW1 gene

Localization
for TYW1 gene

Pathways & Interactions
for TYW1 gene

Drugs & Compounds
for TYW1 gene

Transcripts
for TYW1 gene

Expression
for TYW1 gene

Orthologs
for TYW1 gene

Paralogs
for TYW1 gene

Genomic Variants
for TYW1 gene

Disorders / Diseases
for TYW1 gene

Publications
for TYW1 gene

Intellectual Property
for TYW1 gene

Products
for TYW1 gene