TWISTNB Gene
protein-coding GIFtS: 47
GCID: GC07M019701
|
|
TWIST neighbor
| |
Aliases
for TWISTNB gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| TWIST Neighbor1 2 | | Twist Neighbor Protein2 3 | | DNA-Directed RNA Polymerase I Subunit RPA432 |
Export aliases for TWISTNB gene to outside databasesPrevious GC identifers: GC07M019380 GC07M019482 GC07M019508 |
Summaries
for TWISTNB gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: RPA43_HUMAN, Q3B726Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through itsassociation with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters
|
Genomic Views
for TWISTNB gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000007.13 NC_018918.1 NT_007819.17 NT_079592.2
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the TWISTNB gene promoter:
Max1 ISGF-3 p53 GCNF E4BP4 E47 GCNF-1 GCNF-2 c-Myc RSRFC4
Other transcription factors
Search SABiosciences Chromatin IP Primers for TWISTNB
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TWISTNB |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 7p21.1 Ensembl cytogenetic band: 7p21.1 HGNC cytogenetic band: 7p21.1TWISTNB Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07M019701: view genomic region
(about GC identifiers)
Start:
|
19,735,085 bp from pter |
End:
|
19,748,710 bp from pter |
Size:
|
13,626 bases |
Orientation:
|
minus strand |
1 alternative location:
| Chr7-,CRA_TCAG 19,786,489-19,800,065 |
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Proteins
for TWISTNB gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: RPA43_HUMAN, Q3B726 (See
protein sequence)Recommended Name: DNA-directed RNA polymerase I subunit RPA43 Size: 338 amino acids; 37432 Da
Subunit: Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity).Interacts with RRN3/TIF-IA
Subcellular location: Nucleus, nucleolus (By similarity)
Sequence caution: Sequence=AAH14574.1; Type=Frameshift; Positions=224;
Secondary accessions: A0PJ45 B7Z724Explore the universe of human proteins at neXtProt for TWISTNB: NX_Q3B726
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q3B726
TWISTNB Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_001002926.1
ENSEMBL proteins: ENSP00000222567
Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
TWISTNB for ontologies About GeneDecksing
TWISTNB Antibody Products:
Assay Products for TWISTNB: |
Protein
Domains /
Families
for TWISTNB gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TWISTNB for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q3B726ProtoNet protein and cluster: Q3B726 UniProtKB/Swiss-Prot: RPA43_HUMAN, Q3B726Similarity: Belongs to the eukaryotic RPA43 RNA polymerase subunit family |
Function
for TWISTNB gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: RPA43_HUMAN, Q3B726Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleosidetriphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through itsassociation with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TWISTNB (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TWISTNB
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: TWISTNB (NM_001002926) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TWISTNB | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TWISTNB |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TWISTNB |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0003899 | DNA-directed RNA polymerase activity |
IEA | -- |
TWISTNB for ontologies About GeneDecksing
2 GenomeRNAi human phenotypes for TWISTNB:
|
Pathways & Interactions
for TWISTNB gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TWISTNB
STRING Interaction
Network Preview (showing 3 interactants - click image to see more details)
4 Interacting proteins for TWISTNB (Q3B7263 ENSP000002225674) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006351 | transcription, DNA-dependent |
IEA | -- |
TWISTNB for ontologies About GeneDecksing
|
Drugs & Compounds
for TWISTNB gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for TWISTNB
Search CenterWatch for drugs/clinical trials and news about TWISTNB / RPA43
|
Transcripts
for TWISTNB gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for TWISTNB gene: NM_001002926.1 Unigene Cluster for TWISTNB: TWIST neighbor Hs.353035 [show with all ESTs]Unigene Representative Sequence: NM_0010029262 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000222567(uc003sup.1) ENST00000462263
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TWISTNB (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TWISTNB
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: TWISTNB (NM_001002926) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TWISTNB | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TWISTNB |
Additional cDNA sequence: AK090846.1 AK301329.1 BC013179.1 BC014574.1 BC056267.1 BC070358.1 BC130298.1 BC130300.1 4 DOTS entries: DT.101980128 DT.92422986 DT.100791610 DT.95224858 24/132 AceView cDNA sequences (see all 132): BM454978 BP873431 AA908753 AI091623 AA308959 AA400422 BQ433945 AA078823 AK090846 BX280121 CB143061 CA311137 AA504560 BG178379 BC056267 NM_001002926 AW969056 BU181180 CF529920 BC013179 BI861782 AA464720 BM796798 AA463936
GeneLoc Exon Structure
|
Expression
for TWISTNB gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TWISTNB expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See TWISTNB Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TWISTNB
SOURCE GeneReport for Unigene cluster: Hs.353035
UniProtKB/Swiss-Prot: RPA43_HUMAN, Q3B726Tissue specificity: Widely expressed. Expressed in all fetal and adult tissues tested, with highest expression in fetallung, liver, and kidney, and low expression in all adult tissues
SABiosciences Custom PCR Arrays for TWISTNB
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TWISTNB
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat TWISTNB | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TWISTNB | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TWISTNB | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TWISTNB |
Orthologs
for TWISTNB gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for TWISTNB gene from 6/21 species (see all 21) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
mouse
(Mus musculus) |
Mammalia |
Twistnb1 , 5 |
TWIST neighbor1, 5 |
80.91(n)1
78.64(a)1 |
|
12 (14.38 cM)5
280711 NM_172253.21 NP_758457.11
334296245 |
chicken
(Gallus gallus) |
Aves |
TWISTNB1 |
TWIST neighbor |
66.12(n)
58.55(a) |
|
420603 NM_001199615.1 NP_001186544.1 |
lizard
(Anolis carolinensis) |
Reptilia |
TWISTNB6 |
-- |
59(a) |
1 ↔ 1 |
6(28882086-28886613) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.57762 |
Xenopus laevis transcribed sequence with weak similarity more |
74.45(n) |
|
BJ074347.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
Dr.185562 |
Danio rerio cDNA clone IMAGE6795293, partial cds |
70.78(n) |
|
BC056557.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
CG137736 |
-- |
25(a) |
1 ↔ 1 |
2L(6906666-6907844) |
ENSEMBL Gene Tree for TWISTNB (if available)
TreeFam Gene Tree for TWISTNB (if available) |
Paralogs
for TWISTNB gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for TWISTNB gene
TWISTNB for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for TWISTNB
PGOHUM00000243284
|
Genomic Variants
for TWISTNB gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 7 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for TWISTNB (19735085 - 19748710 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for TWISTNB: -- | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases
for TWISTNB gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TWISTNB for disorders About GeneDecksing
OMIM gene information: 608312
OMIM disorders: --
2  diseases for TWISTNB: About MalaCardssaethre-chotzen syndrome learning disability
2 diseases from the University of Copenhagen DISEASES database for TWISTNB:Saethre-Chotzen syndrome Learning disability Export disorders for TWISTNB gene to outside databases
|
Publications
for TWISTNB gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TWISTNB gene, integrated from 9 sources (see all 16):
(articles sorted by number of sources associating them with TWISTNB) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Identification and characterisation of the gene TWIST NEIGHBOR (TWISTNB) located in the microdeletion syndrome 7p21 region. (PubMed id 12438708)1, 2, 3 Kosan C. and Kunz J. (2002)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)1, 2 Scherer S.W.... Tsui L.-C. (2003)
- Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter. (PubMed id 12393749)1, 2 Yuan X.... Grummt I. (2002)
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692)2 Rigbolt K.T....Blagoev B. (2011)
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
- Hundreds of variants clustered in genomic loci and bi ological pathways affect human height. (PubMed id 20881960)1 Lango Allen H....Hirschhorn J.N. (2010)
- Defining the human deubiquitinating enzyme interactio n landscape. (PubMed id 19615732)1 Sowa M.E....Harper J.W. (2009)
- Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
- Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. (PubMed id 18220336)2 Cantin G.T....Yates J.R. III (2008)
|
External Searches for TWISTNB gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing TWISTNB gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TWISTNB gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TWISTNB gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for TWISTNB | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for TWISTNB gene:
Search GeneIP for patents involving TWISTNB
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for TWISTNB gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat TWISTNB | | | Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TWISTNB | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TWISTNB | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TWISTNB | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TWISTNB | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TWISTNB |
| |
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| |  |  |  |  | | | | |
 |
 |
 |
 | | | TWISTNB Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TWISTNB |
|  |  |  | | | | ThermoFisher Antibodies for TWISTNB |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TWISTNB |
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