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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TUBGCP3 Gene

protein-coding   GIFtS: 52
GCID: GC13M113139

tubulin, gamma complex associated protein 3

 Explore 3 diseases affiliated with
TUBGCP3 via our new
 Human Malady Compendium 
Biological research products
for TUBGCP3
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tubulin, Gamma Complex Associated Protein 31 2     GCP-32 3
GCP31 2 3     H104p1
SPBC981 2     HGCP31
Spc98p1 2     HGrip1041
Gamma-Ring Complex Protein 104 KDa2 3     HSpc981
Spindle Pole Body Protein Spc98 Homolog2 3     Gamma-Tubulin Complex Component 32

External Ids:    HGNC: 185981   Entrez Gene: 104262   Ensembl: ENSG000001262167   UniProtKB: Q96CW53   

Export aliases for TUBGCP3 gene to outside databases

Previous GC identifers: GC13M111749 GC13M107776 GC13M112225 GC13M111087 GC13M112187 GC13M093580


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: GCP3_HUMAN, Q96CW5
Function: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Gene Wiki entry for TUBGCP3


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000013.10  NC_018924.1  NT_027140.6  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TUBGCP3 gene promoter:
         GR   PPAR-alpha   GR-alpha   YY1   En-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTUBGCP3 promoter sequence
   Search SABiosciences Chromatin IP Primers for TUBGCP3

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TUBGCP3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 13q34   Ensembl cytogenetic band:  13q34   HGNC cytogenetic band: 13q34

TUBGCP3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TUBGCP3 gene location

GeneLoc information about chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13M113139:  view genomic region     (about GC identifiers)

Start:
113,139,325 bp from pter      End:
113,242,481 bp from pter
Size:
103,157 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: GCP3_HUMAN, Q96CW5 (See protein sequence)
Recommended Name: Gamma-tubulin complex component 3  
Size: 907 amino acids; 103571 Da
Subunit: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts
with CDK5RAP2; the interaction is leading to centrosomal localization of TUBGCP3 and CDK5RAP2
Subcellular location: Cytoplasm, cytoskeleton, centrosome
Secondary accessions: O43631 O60852 O60853 Q5T8L2 Q7Z4K1 Q96I79
Alternative splicing: 3 isoforms:  Q96CW5-1   Q96CW5-2   Q96CW5-3   

Explore the universe of human proteins at neXtProt for TUBGCP3: NX_Q96CW5

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96CW5

  • TUBGCP3 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_006313.1  
    ENSEMBL proteins: 
     ENSP00000261965   ENSP00000364821  
    Reactome Protein details: Q96CW5
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    Browse Proteins at Uscn

    Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA9566969
    GO:0005813centrosome NAS11694571
    GO:0005814centriole IDA9566969
    GO:0005819spindle NAS7876350
    GO:0005827polar microtubule IDA9566969


    TUBGCP3 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TUBGCP3 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR015697 GCP3
     IPR007259 Spc97_Spc98

    Graphical View of Domain Structure for InterPro Entry Q96CW5

    ProtoNet protein and cluster: Q96CW5

    1 Blocks protein family: IPB007259 Spc97/Spc98

    UniProtKB/Swiss-Prot: GCP3_HUMAN, Q96CW5
    Similarity: Belongs to the TUBGCP family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: GCP3_HUMAN, Q96CW5
    Function: Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

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    hsa-miR-30c hsa-miR-495 hsa-miR-520d-5p hsa-miR-199b-3p hsa-miR-199a-3p hsa-miR-3129-5p hsa-miR-30d hsa-miR-410
    SwitchGear 3'UTR luciferase reporter plasmidTUBGCP3 3' UTR sequence
    Inhib. RNA
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TUBGCP3

    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005198structural molecule activity NAS9566967
    GO:0005200structural constituent of cytoskeleton NAS9566969
    GO:0005515protein binding IPI17959831
    GO:0043015gamma-tubulin binding IDA9566969


    TUBGCP3 for ontologies           About GeneDecksing


    5 GenomeRNAi human phenotypes for TUBGCP3:
     Decreased viability of wild-ty  Decreased viability with pacli  Increased gamma-H2AX phosphory  Synthetic lethal with Ras 
     Synthetic lethal with c-Myc af 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways - 5/6 super-pathways (see all 6About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Recruitment of mitotic centrosome proteins and complexes
    Centrosome maturation1.00
    G2/M Transition0.83
    Recruitment of mitotic centrosome proteins and complexes1.00
    Mitotic G2-G2/M phases0.81
    2Cell Cycle
    Cell Cycle1.00
    Cell Cycle, Mitotic0.84
    3Nanog in Mammalian ESC Pluripotency
    14-3-3 Induced Intracellular Signaling0.59
    4Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane
    Recruitment of NuMA to mitotic centrosomes0.50
    5Ubiquitinated Orc1 is degraded by the proteasome
    Parkinson's Disease Pathway0.31

    Pathway sources
    See GeneCards unified pathways
    Show all pathways

    3 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for TUBGCP3
        14-3-3 Induced Intracellular Signaling
    Hedgehog Signaling in Mammals
    Parkinson's Disease Pathway

    5/7        Reactome Pathways for TUBGCP3 (see all 7)
        Centrosome maturation
    G2/M Transition
    Cell Cycle
    Recruitment of mitotic centrosome proteins and complexes
    Mitotic G2-G2/M phases



    TUBGCP3 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for TUBGCP3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/821 Interacting proteins for TUBGCP3 (Q96CW52, 3 ENSP000002619654) via UniProtKB, MINT, STRING, and/or I2D (see all 821)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    PCNTO956132, 3, ENSP000003525724MINT-7947479 I2D: score=3 STRING: ENSP00000352572
    IMMTQ168912, 3, ENSP000003872624MINT-8276031 I2D: score=2 STRING: ENSP00000387262
    TUBG1P232582, 3, ENSP000002514134MINT-7945693 MINT-7947479 I2D: score=5 STRING: ENSP00000251413
    TUBGCP2Q9BSJ22, 3, ENSP000002529364MINT-7945693 MINT-7947479 I2D: score=3 STRING: ENSP00000252936
    CKAP5Q140082, ENSP000003465664MINT-7945693 MINT-7947479 STRING: ENSP00000346566
    About this table

    Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000086G2/M transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0007020microtubule nucleation NAS9566969
    GO:0007338single fertilization NAS9138452


    TUBGCP3 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    TUBGCP3 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
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    Browse Tocris compounds for TUBGCP3
    1 Novoseek chemical compound relationship for TUBGCP3 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    sucrose 36.2 1 9566967 (1)

    Search CenterWatch for drugs/clinical trials and news about TUBGCP3 / GCP3 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TUBGCP3 gene: 
    NM_006322.4  

    Unigene Clusters for TUBGCP3:

    Tubulin, gamma complex associated protein 3
    Hs.224152  [show with all ESTs], Hs.595145  [show with all ESTs]
    Unigene Representative Sequences: BC046634, BE350321
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000261965(uc001vse.1 uc010tjq.1) ENST00000469302 ENST00000375669(uc001vsf.3)
    ENST00000462580 ENST00000464139(uc001vsg.1) ENST00000483532

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    hsa-miR-30c hsa-miR-495 hsa-miR-520d-5p hsa-miR-199b-3p hsa-miR-199a-3p hsa-miR-3129-5p hsa-miR-30d hsa-miR-410
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    Additional cDNA sequence: L13800.1 

    11 DOTS entries:

    DT.113239  DT.100788660  DT.100788666  DT.40121013  DT.95179955  DT.95214519  DT.100788662  DT.120763339 
    DT.91886396  DT.95179958  DT.95363972 

    24/154 AceView cDNA sequences (see all 154):

    AF042378 BC013781 CA392672 AA720688 AA233670 BQ438247 BM474805 AI821622 
    AI268652 Z45067 AL703466 BU742889 BM479981 L13801 CR616288 CD676051 
    BT009772 BU630764 CK818925 BU527799 BU623732 BQ223303 AI682187 BI461265 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TUBGCP3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GATAATGAAG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See TUBGCP3 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TUBGCP3

    SOURCE GeneReport for Unigene clusters: Hs.224152 Hs.595145

    UniProtKB/Swiss-Prot: GCP3_HUMAN, Q96CW5
    Tissue specificity: Ubiquitously expressed

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for TUBGCP3 gene from 7/30 species (see all 30)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves TUBGCP31 tubulin, gamma complex associated protein 3 81.24(n)
    91.5(a)
      418750  XM_416949.3  XP_416949.2 
    lizard
    (Anolis carolinensis)
    Reptilia TUBGCP36
    --
    86(a)
    1 ↔ 1
    3(105517403-105584701)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC691342 hypothetical protein MGC69134 78.75(n)    BC057755.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufc09h082 Transcribed sequence with moderate similarity to protein more 75.97(n)    BI476157.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta l(1)dd41 lethal (1) discs degenerate 4 46.32(n)
    37.42(a)
      48481  NM_080180.3  NP_524919.2 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons SPC981 spindle pole body component 98 47.87(n)
    39.24(a)
      830557  NM_120751.3  NP_196286.1 
    rice
    (Oryza sativa)
    Liliopsida Os09g04462001 hypothetical protein 46.39(n)
    37.04(a)
      4347202  NM_001069843.2  NP_001063308.2 


    ENSEMBL Gene Tree for TUBGCP3 (if available)
    TreeFam Gene Tree for TUBGCP3 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TUBGCP3 gene
    TUBGCP62  TUBGCP42  TUBGCP22  

    TUBGCP3 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for TUBGCP3
    PGOHUM00000248685


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    3 NCBI SNPs in TUBGCP3 are shown     About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 13 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs342642691,2
    C--113158800(+) ATCTC-/AAAAAA 1 -- int10--------
    rs2022318461,2
    ----113232532(+) TGCTG-/GC    
       TCCTT
    GCTCC
    1 -- int10--------
    rs605501511,2
    ----113232536(+) TGCTC-/CT    
       TGCTC
    TCCCA
    1 -- int10--------

    HapMap Linkage Disequilibrium report for TUBGCP3 (113139325 - 113242481 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 5 variations for TUBGCP3
         2 CNVs: 71799 101870
         3 Indels: 60136 61363 45127

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TUBGCP3 for disorders           About GeneDecksing

    3 diseases for TUBGCP3:    About MalaCards
    mantle cell lymphoma    infertility    parkinson's disease

    Human Genome Epidemiology (HuGE) Navigator: TUBGCP3 (2 documents)

    Export disorders for TUBGCP3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TUBGCP3 gene, integrated from 9 sources (see all 31):
    (articles sorted by number of sources associating them with TUBGCP3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p. (PubMed id 9566967)1, 2, 3, 9 Murphy S.M.... Stearns T. (1998)
    2. Characterization of the human homologue of the yeast spc98p and its association with gamma-tubulin. (PubMed id 9566969)1, 2, 3, 9 Tassin A.-M.... Bornens M. (1998)
    3. CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex. (PubMed id 17959831)1, 2 Fong K.W....Qi R.Z. (2008)
    4. The DNA sequence and analysis of human chromosome 13. (PubMed id 15057823)1, 2 Dunham A.... Ross M.T. (2004)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    6. Stability of the small gamma-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus. (PubMed id 19299467)1, 9 Fant X....Merdes A. (2009)
    7. GCP5 and GCP6: two new members of the human gamma-tubulin complex. (PubMed id 11694571)1, 9 Murphy S.M.... Stearns T. (2001)
    8. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    9. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    10. A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 10426 HGNC: 18598 AceView: TUBGCP3 Ensembl:ENSG00000126216 euGenes: HUgn10426
    ECgene: TUBGCP3 H-InvDB: TUBGCP3

    (According to HUGE)
    About This Section
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    NameDescription
    PharmGKB entry for TUBGCP3 Pharmacogenomics, SNPs, Pathways

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