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TUBE1 Gene

protein-coding   GIFtS: 51

GC06M112498
tubulin, epsilon 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
Epsilon-tubulin 3
FLJ22589 1, 2
FLJ44203 2
OTTHUMP00000017033 2
OTTHUMP00000040425 2
TUBE 1, 2, 3
dJ142L7.2 1, 2
Descriptions
Tubulin epsilon chain 2
tubulin, epsilon 1 2
External Ids
HGNC: 207751
Entrez Gene: 511752
UniProtKB: Q9UJT03
Ensembl: ENSG000000749357
Search outside databases for aliases for TUBE1 gene

Previous GC identifers: GC06M112414 GC06M112437

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for TUBE1:
This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar
sub-distal appendages that are associated with the older of the two centrioles after centrosome
duplication. This protein plays a central role in organization of the microtubules during
centriole duplication. A pseudogene of this gene is found on chromosome 5.

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TUBE1 gene  

Entrez Gene cytogenetic band: 6q21   Ensembl cytogenetic band:  6q21   HGNC cytogenetic band: 6q21

TUBE1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M112498:     (about GC identifiers)

Start:
112,498,673 bp from pter
End:
112,515,375 bp from pter
Size:
16,703 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000006.10  NT_025741.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TBE_HUMAN, Q9UJT0 (See protein sequence)
Recommended Name: Tubulin epsilon chain  
Size: 475 amino acids; 52932 Da
Subcellular location: Centrosome. Note=Associated with pericentriolar material
Secondary accessions: Q8NEG3

REFSEQ proteins: NP_057346.1  

ENSEMBL proteins: 
ENSP00000357651 ENSP00000357647 ENSP00000357646 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (epsilon Tubulin)
Human Recombinant Proteins from Abnova (TUBE1)
              OriGene Purified Recombinant Human Protein: TUBE1 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000242 pericentriolar material TAS10620804
GO:0005874 microtubule IEA--
GO:0043234 protein complex IEA--
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Antibodies for TUBE1: 
Invitrogen Antibodies for TUBE1
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibody Arrays and Antibodies for TUBE1
Browse R&D Systems for Antibodies
Antibodies from Abcam (epsilon Tubulin), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (TUBE1)
Novus Biologicals Antibody for TUBE1

Assays for TUBE1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5 InterPro domains/families:
 IPR000217 Tubulin
 IPR003008 Tubulin_FtsZ_GTPase
 IPR004057 Epsilon_tubulin
 IPR018316 Tubulin/FtsZ_2-layer-sand-dom
 IPR017975 Tubulin_CS


   GeneDecks  TUBE1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9UJT0

ProtoNet protein and cluster: Q9UJT0

1 Blocks protein family: IPB004057 Epsilon-tubulin signature

UniProtKB/Swiss-Prot: TBE_HUMAN, Q9UJT0
Similarity: Belongs to the tubulin family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TUBE1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TUBE1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016262

              Applied Biosystems Silencer® siRNAs for TUBE1

              Sigma-Aldrich siRNA and siRNA Panels for TUBE1  
                     Sigma-Aldrich shRNA for TUBE1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for TUBE1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_016262
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_016262
                                 untagged cDNA clone in CMV expression vector: NM_016262 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_016262

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0003924 GTPase activity IEA--
GO:0005200 structural constituent of cytoskeleton TAS10620804
GO:0005525 GTP binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --

3 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007018 microtubule-based movement IEA--
GO:0007098 centrosome cycle TAS10620804
GO:0051258 protein polymerization IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Enzo Life Sciences drugs & compounds for TUBE1
Browse Small Molecules at Sigma-Aldrich

Compounds for TUBE1 available from Tocris Bioscience
CompoundAction CAS number
XRP44XRas-Net pathway inhibitor[729605-21-4]
Vinblastine sulfateDisrupts microtubules[143-67-9]
TaxolPromotes assembly and inhibits disassembly of microtubules[33069-62-4]
NocodazoleMicrotubule inhibitor[31430-18-9]
Vincristine sulfateDisrupts microtubules[2068-78-2]
About this table



(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TUBE1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TUBE1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_016262

              Sigma-Aldrich siRNA and siRNA Panels for TUBE1  
                     Sigma-Aldrich shRNA for TUBE1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_016262  

REFSEQ mRNAs for TUBE1 gene: 

NM_016262.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_016262  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_016262
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_016262
                                 untagged cDNA clone in CMV expression vector: NM_016262 

Additional cDNA sequence: 

AF201334.1 AK026242.1 AK126191.1 BC025405.2 BC031101.1 CR609343.1 CR613971.1 

12 DOTS entries:

DT.412752  DT.65287297  DT.100666807  DT.311146  DT.91725620  DT.121321733  DT.206223  DT.86852069 
DT.91725623  DT.95190331  DT.100727407  DT.92007628 

24/147 AceView cDNA sequences (see all 147 ):

CA411886 AF201334 BE221549 CR613971 AL580253 AI613127 BF058163 CB163568 
AK026242 AA768510 AL598964 BM264176 AW294927 CB153775 BG108376 N22140 
AA099692 AV658536 AI805900 AI243385 AK126191 BM509957 AL134732 AI492047 

highest scoring ESTs for TUBE1:

AA215502 AA973387 AF201334 AI302255 AI333177 AI492047 AI768712 AI801903 AK026242 AL580253 

Unigene Cluster for TUBE1:

Tubulin, epsilon 1
Hs.34851  [show with all ESTs]
Unigene Representative Sequence: AK126191


GeneLoc Exon Structure

5/8 Alternative Splicing Database (ASD) splice patterns (SP) for TUBE1 (see all 8 )

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12
SP1:                                      -                 -                 -     -           -           -                           
SP2:                                      -           -     -                 -     -           -           -                           
SP3:                                      -                 -                 -     -                                                   
SP4:                                      -                 -                                                                           
SP5:                                      -           -     -                                                                           

About this scheme

ECgene alternative splicing isoforms for TUBE1

3 Ensembl transcripts including schematic representations:
ENST00000368662  ENST00000368658  ENST00000368657  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

TUBE1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for TUBE1

1 / 2 / 3

5 probe-sets matching TUBE1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

68971_r_at2, 3 U95-D 1 1.00 1.00 0.27 0.25 AI679686 0.60 1.00 0.82 1
45120_at2, 3 U95-B 1 1.00 1.00 0.88 1.70 AA587950 0.80 0.80 0.80 1
50158_at2, 3 U95-C 1 0.69 1.00 0.81 1.10 AA588685 0.60 1.00 0.82 1

226181_at2, 3 U133-B 1 1.00 1.00 -- -- AI613127 0.60 0.75 0.68 1

226181_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  TUBE1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TATTTGTATT

SOURCE GeneReport for Unigene cluster: Hs.34851
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for TUBE1 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TUBE11   -- tubulin, epsilon 1 91.09(n)
93.26(a)
612390  XM_850121.1  XP_855214.1 
chimpanzee
(Pan troglodytes)
TUBE11   -- tubulin, epsilon 1 99.72(n)
99.79(a)
472096  XM_001159684.1  XP_001159684.1 
cow
(Bos taurus)
TUBE11   -- tubulin, epsilon 1 91.16(n)
93.26(a)
512715  XM_001251130.1  XP_001251131.1 
rat
(Rattus norvegicus)
Tube11   -- tubulin, epsilon 1 84.04(n)
90.93(a)
361856  XM_342151.3  XP_342152.3 
mouse
(Mus musculus)
Tube11, 5 105
epsilon-tubulin 11, 5 85.4(n)1
91.16(a)1
719241  NM_028006.21  NP_082282.11 
 AI5513435  AK0100055  (see all 14)
About this table        Species with no ortholog for TUBE1

ENSEMBL Gene Tree for TUBE1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for TUBE1 gene
TUBG12  TUBG22  TUBD12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/57 NCBI SNPs in TUBE1 are shown (see all 57 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 72)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 6 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs9715311,2
A,C,F,H,O112498226(+) TGATAC/AATCAT 1 -- ng5110Minor allele frequency- A:0.33MN EU EA WA NA 926
rs68997271,2
H112499268(+) AAAAAA/GCAATG 1 -- ut314Minor allele frequency- G:0.00EU EA WA 418
--
rs178569201,2
H112499330(+) GTCTGG/CGTAAA 1 R/P mis14Minor allele frequency- C:0.00EU EA WA 420
rs17098651,2
A,C,F,H,O112507135(+) CATGAG/ACACGT 1 -- int18Minor allele frequency- A:0.47MN NA EU EA WA 744
rs111533351,2
A,C,F,H112505143(+) ATTAAG/ACATGC 1 -- int17Minor allele frequency- A:0.25EU EA WA NA 552
rs37983631,2
A,C,F,H112501106(-) AGTTTC/AAAAAA 1 -- int18Minor allele frequency- A:0.27EA NA EU WA 2046
rs37568861,2
A,C,F,H112507918(-) AAGATG/AAATTT 1 -- int111Minor allele frequency- A:0.49NA EU EA WA 970
rs16248141,2
A,C,F,H112504138(+) GAGAAC/TGTTTC 1 -- int113Minor allele frequency- T:0.29MN EA NA EU WA 1844
rs104568771,2
C,F,H112506443(+) CTGATC/TATCTG 1 -- int17Minor allele frequency- T:0.29EU EA WA NA 552
rs170732771,2
C,F,H112500086(+) ATCTAC/ATTGCT 1 -- int111Minor allele frequency- A:0.02NA EU EA WA 976
About this table

HapMap Linkage Disequilibrium images for TUBE1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 607345

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/15 PubMed articles for TUBE1 gene (see all 15 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 51175 HGNC: 20775 AceView: TUBE1 Ensembl:ENSG00000074935 euGenes: HUgn51175
ECgene: TUBE1 H-InvDB: TUBE1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for TUBE1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for TUBE1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for TUBE1 Antibodies for TUBE1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for TUBE1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
 Antibodies   Primer Pairs  
 Cell Culture Products   ELISAs  
 Flow Cytometry Kits   Protease Activity Assays & Reagents  
 Cell Selection/Detection Kits & Reagents   ELISA/Assay Development Kits & Reagents  
 Multiplex/Array Assay Kits & Reagents   ELISpot Kits & Development Modules  
 Recombinant & Natural Proteins  

 Recombinant Proteins (TUBE1)
 Antibodies (TUBE1)
 Chimera RNAi (TUBE1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
 Search for Antibodies & Assays

 Recombinant Proteins
(epsilon Tubulin)
 Antibodies (epsilon Tubulin)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Tocris compounds for TUBE1




 Drugs for TUBE1
 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for TUBE1

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