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TUBA8 Gene

protein-coding   GIFtS: 63

GC22P016968
tubulin, alpha 8
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TUBAL2)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
OTTHUMP00000196216 2
TUBAL2 2, 3, 5
Descriptions
Alpha-tubulin 8 3
Tubulin alpha chain-like 2 3
tubulin, alpha 8 2
tubulin, alpha-like 2 2
External Ids
HGNC: 124101
Entrez Gene: 518072
UniProtKB: Q9NY653
Ensembl: ENSG000001837857
Search outside databases for aliases for TUBA8 gene

Previous GC identifer: GC22P015533

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: TBA8_HUMAN, Q9NY65
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an
exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Gene Wiki entry for TUBA8

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TUBA8 gene  

Entrez Gene cytogenetic band: 22q11.1   Ensembl cytogenetic band:  22q11.21   HGNC cytogenetic band: 22q11

TUBA8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 22         GeneLoc Exon Structure

GeneLoc location for GC22P016968:     (about GC identifiers)

Start:
16,973,453 bp from pter
End:
16,994,498 bp from pter
Size:
21,046 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000022.9  NT_011519.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TBA8_HUMAN, Q9NY65 (See protein sequence)
Recommended Name: Tubulin alpha-8 chain  
Size: 449 amino acids; 50094 Da
Subunit: Dimer of alpha and beta chains
Miscellaneous: This tubulin does not have a C-terminal tyrosine
Secondary accessions: Q2M3N4

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_061816.1  

    ENSEMBL proteins: 
    ENSP00000318575 ENSP00000333326 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Human Recombinant Proteins from Abnova (TUBA8)
                  OriGene Purified Recombinant Human Protein: TUBA8 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005874 microtubule IEA--
    GO:0043234 protein complex IEA--
    About this table

    Antibodies for TUBA8: 
    Invitrogen Antibodies for TUBA8
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for TUBA8

    Assays for TUBA8: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR002452 Alpha_tubulin
     IPR000217 Tubulin
     IPR003008 Tubulin_FtsZ_GTPase
     IPR018316 Tubulin/FtsZ_2-layer-sand-dom
     IPR017975 Tubulin_CS


       GeneDecks  TUBA8 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9NY65

    ProtoNet protein and cluster: Q9NY65

    1 Blocks protein family: IPB002452 Alpha tubulin signature

    UniProtKB/Swiss-Prot: TBA8_HUMAN, Q9NY65
    Similarity: Belongs to the tubulin family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TUBA8)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TUBA8)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018943

                  Applied Biosystems Silencer® siRNAs for TUBA8

                  Sigma-Aldrich siRNA and siRNA Panels for TUBA8  
                         Sigma-Aldrich shRNA for TUBA8  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for TUBA8
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018943
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018943
                                     untagged cDNA clone in CMV expression vector: NM_018943 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_018943

    UniProtKB/Swiss-Prot: TBA8_HUMAN, Q9NY65
    Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an
    exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0003924 GTPase activity IEA--
    GO:0005198 structural molecule activity IEA--
    GO:0005525 GTP binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    5/9 Millipore Pathways for TUBA8 (see all 9 )
     Cytoskeleton remodeling Neurofilaments
     Cytoskeleton remodeling Slit-Robo signaling
     Cytoplasmic microtubules
     Cytoskeleton remodeling Reverse signaling by ephrin B
     Cytoskeleton remodeling Keratin filaments

       GeneDecks  TUBA8 for the pathways selected above  
    About GeneDecksing

    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TUBA8  (Your Favorite Gene powered by Ingenuity) 
     14-3-3-mediated Signaling
     Germ Cell-Sertoli Cell Junction Signaling

       GeneDecks  TUBA8 for the pathways selected above  
    About GeneDecksing

    3 Kegg Pathways  (Kegg details for TUBA8):
     hsa04540 Gap junction
     hsa05130 Pathogenic Escherichia coli infection - EHEC
     hsa05131 Pathogenic Escherichia coli infection - EPEC

       GeneDecks  TUBA8 for the pathways selected above  
    About GeneDecksing

    5/31 Interacting proteins for TUBA8 (ENSP000003185753 Q9NY652) via UniProtKB, MINT, and/or STRING (see all 31 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    IKBKGQ9Y6K92MINT-48217
    PEX6ENSP000003035113STRING (score=.982)
    PEX3ENSP000003565633STRING (score=.978)
    STMN1ENSP000003505313STRING (score=.961)
    PEX13ENSP000002950303STRING (score=.922)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007018 microtubule-based movement IEA--
    GO:0051258 protein polymerization IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for TUBA8
    Browse Small Molecules at Sigma-Aldrich

    Compounds for TUBA8 available from Tocris Bioscience
    CompoundAction CAS number
    XRP44XRas-Net pathway inhibitor[729605-21-4]
    Vinblastine sulfateDisrupts microtubules[143-67-9]
    TaxolPromotes assembly and inhibits disassembly of microtubules[33069-62-4]
    NocodazoleMicrotubule inhibitor[31430-18-9]
    Vincristine sulfateDisrupts microtubules[2068-78-2]
    About this table



    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TUBA8)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TUBA8)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_018943

                  Sigma-Aldrich siRNA and siRNA Panels for TUBA8  
                         Sigma-Aldrich shRNA for TUBA8  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_018943  

    REFSEQ mRNAs for TUBA8 gene: 

    NM_018943.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_018943  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_018943
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_018943
                                     untagged cDNA clone in CMV expression vector: NM_018943 

    Additional cDNA sequence: 

    AJ245922.1 AK056947.1 AK315316.1 BC074827.2 BC104845.1 BX537702.1 CR456600.1 CR620426.1 

    4 DOTS entries:

    DT.428261  DT.95253738  DT.100658425  DT.75173237 

    24/181 AceView cDNA sequences (see all 181 ):

    CA433269 BQ100690 BC047320 CA427902 AA676826 AI869536 BQ575902 C21355 
    BC016280 AJ245922 AI636019 BC074827 CA424779 F30278 BF059267 CR620426 
    BI917830 NM_018943 H55532 AW235279 BX537702 F36680 CA433324 BQ943945 

    highest scoring ESTs for TUBA8:

    AA180063 AJ245922 AJ572492 AK056947 BF195571 BM511724 BM670545 CR456600 F16034 F22723 

    Unigene Cluster for TUBA8:

    Tubulin, alpha 8
    Hs.137400  [show with all ESTs]
    Unigene Representative Sequence: CR620426


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for TUBA8

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c
    SP1:                    -                                                   
    SP2:                                                                        
    SP3:                                                  -                     
    SP4:                                                                        

    About this scheme

    ECgene alternative splicing isoforms for TUBA8

    2 Ensembl transcripts including schematic representations:
    ENST00000316027  ENST00000330423  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    TUBA8 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for TUBA8

    1 / 2 / 3

    3 probe-sets matching TUBA8 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    61935_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AA180063 1.00 0.90 0.95 1

    220069_at2, 3 U133-A 1 1.00 1.00 -- -- NM_018943 0.60 0.83 0.73 1

    220069_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TACGCCAAGC

    SOURCE GeneReport for Unigene cluster: Hs.137400

    UniProtKB/Swiss-Prot: TBA8_HUMAN, Q9NY65
    Tissue specificity: Preferentially expressed in heart, skeletal muscle and testis

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for TUBA8 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    TUBA81   -- tubulin, alpha 8 92.13(n)
    98.89(a)
    486762  XM_543889.2  XP_543889.2 
    chimpanzee
    (Pan troglodytes)
    TUBA81   -- tubulin, alpha 8 99.63(n)
    100(a)
    458641  XM_001164109.1  XP_001164109.1 
    cow
    (Bos taurus)
    TUBA81   -- tubulin, alpha 8 89.9(n)
    99.11(a)
    768036  NM_001077095.1  NP_001070563.1 
    rat
    (Rattus norvegicus)
    Tuba81   -- tubulin, alpha 8 89.53(n)
    99.11(a)
    500377  NM_001024339.1  NP_001019510.1 
    mouse
    (Mus musculus)
    Tuba81, 5 65
    tubulin, alpha 81, 5 89.53(n)1
    99.33(a)1
    538571  NM_017379.11  NP_059075.11 
     AA0639145  AA4039275  (see all 16)
    About this table        Species with no ortholog for TUBA8

    ENSEMBL Gene Tree for TUBA8
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for TUBA8 gene
    TUBA3C2  TUBA3E2  TUBA3D2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/175 NCBI SNPs in TUBA8 are shown (see all 175 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 99)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 22 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs25406201,2
    A,C,F,H,O16994874(+) CTTATA/CTCCAA 1 -- ng3112Minor allele frequency- C:0.47EU EA WA NA 1120
    rs22343331,2
    C,F,H16989647(+) CAGCCA/GGATGG 1 Q/R mis1 ese312Minor allele frequency- G:0.03NA EU EA WA 1160
    rs119136201,2
    C,F,H16972410(+) AGAAAT/GCTGCT 1 -- ng514Minor allele frequency- G:0.01EU EA WA 418
    rs22343311,2
    C,H,O16989128(+) AGATGC/TTTGCT 1 A/V mis14Minor allele frequency- T:0.00EU EA WA 418
    rs121599751,2
    C,F,H16994445(+) CGAGAC/TGAACA 1 -- ut314Minor allele frequency- T:0.05EU EA WA 420
    rs3621891,2
    C,F,H16994407(+) CtgtcC/Ttaggc 1 -- ut316Minor allele frequency- T:0.00MN EU EA WA 784
    --
    rs57474801,2
    A,C16971757(+) aaaaaA/Gaaaga 1 -- ng51 trp30--------
    rs81423361,2
    F,H16972194(+) ATGTGC/ATTGGC 1 -- ng513Minor allele frequency- A:0.01EA WA 298
    rs22343401,2
    F,H16994047(+) AGGGTG/TGCACG 1 -- ut314Minor allele frequency- T:0.02EU EA WA 420
    --
    rs4258891,2
    A,C16971891(+) gtgaaA/Gccccg 1 -- ng511Minor allele frequency- G:0.00EA 180
    About this table

    HapMap Linkage Disequilibrium images for TUBA8 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 605742

    Genetic Association Database: TUBA8
    Human Genome Epidemiology Navigator: TUBA8 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/20 PubMed articles for TUBA8 gene (see all 20 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 51807 HGNC: 12410 AceView: PEX26andTUBA8 Ensembl:ENSG00000183785 euGenes: HUgn51807
    ECgene: TUBA8 H-InvDB: TUBA8
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TUBA8:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for TUBA8-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for TUBA8 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for TUBA8 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Tagged/untagged cDNA clones
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Tocris compounds for TUBA8




     Drugs for TUBA8
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Search Novus for antibodies for TUBA8

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