TTL Gene
protein-coding GIFtS: 49
GCID: GC02P113239
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|
tubulin tyrosine ligase
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Aliases
for TTL gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Tubulin Tyrosine Ligase1 2 | | EC 6.3.2.253 8 | | MGC462351 | | 2410003M22Rik2 | | Tubulin--Tyrosine Ligase2 | | Tubulin-Tyrosine Ligase2 |
Export aliases for TTL gene to outside databasesPrevious GC identifers: GC02P113146 GC02P113334 GC02P112956 GC02P105693 |
Summaries
for TTL gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for TTL:
TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529).Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubuledisassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination andtyrosination (Erck et al., 2003 (PubMed 14571137)).(supplied by OMIM, Mar 2008)
UniProtKB/Swiss-Prot: TTL_HUMAN, Q8NG68Function: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin(By similarity)
|
Genomic Views
for TTL gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000002.11 NC_018913.1 NT_022135.16
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the TTL gene promoter:
HFH-3 AhR Brachyury HNF-3beta Arnt FOXI1 TFIID Zic1 COMP1 Pax-4a
Other transcription factors
Search SABiosciences Chromatin IP Primers for TTL
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TTL |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 2q13 Ensembl cytogenetic band: 2q13 HGNC cytogenetic band: 2q13TTL Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 2 GeneLoc Exon Structure GeneLoc location for GC02P113239: view genomic region
(about GC identifiers)
Start:
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113,239,731 bp from pter |
End:
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113,290,227 bp from pter |
Size:
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50,497 bases |
Orientation:
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plus strand |
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Proteins
for TTL gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: TTL_HUMAN, Q8NG68 (See
protein sequence)Recommended Name: Tubulin--tyrosine ligase Size: 377 amino acids; 43212 Da
Cofactor: Magnesium (By similarity)
Cofactor: Potassium (By similarity)
Subunit: Monomer (By similarity)
Secondary accessions: Q585T3 Q7Z302 Q8N426Explore the universe of human proteins at neXtProt for TTL: NX_Q8NG68
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8NG68
4/11 DME Specific Peptides for TTL (Q8NG68) (see all 11)
TTL Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_714923.1
ENSEMBL proteins: ENSP00000233336
Human Recombinant Protein Products:
TTL Antibody Products:
Assay Products for TTL: |
Protein
Domains /
Families
for TTL gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TTL for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q8NG68ProtoNet protein and cluster: Q8NG68 1 Blocks protein family: IPB004344 Tubulin-tyrosine ligase
UniProtKB/Swiss-Prot: TTL_HUMAN, Q8NG68Similarity: Belongs to the tubulin--tyrosine ligase familySimilarity: Contains 1 TTL domain |
Function
for TTL gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: TTL_HUMAN, Q8NG68Function: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin(By similarity)Catalytic activity: ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate Genatlas biochemistry entry for TTL:tetracycline transporter-like
Enzyme Number (IUBMB): EC 6.3.2.251 2
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TTL (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TTL
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): TTL (NM_005371) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TTL | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TTL |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TTL |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004835 | tubulin-tyrosine ligase activity |
IEA | -- | | GO:0005524 | ATP binding |
IEA | -- |
TTL for ontologies About GeneDecksing
Animal Models:
Mouse knock-out Ttltm1Wehl for TTL
5 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Ttl):
TTL for phenotypes About GeneDecksing
|
Pathways & Interactions
for TTL gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TTL
STRING Interaction
Network Preview (showing 1 interactants - click image to see more details)
1 Interacting protein for TTL (ENSP000002333364) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0000226 | microtubule cytoskeleton organization |
IEA | -- | | GO:0006464 | cellular protein modification process |
IEA | -- | | GO:0030516 | regulation of axon extension |
IEA | -- |
TTL for ontologies About GeneDecksing
|
Drugs & Compounds
for TTL gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
TTL for compounds About GeneDecksing
 Browse Tocris compounds for TTL
6 HMDB Compounds for TTL About this table
2 Novoseek chemical compound relationships for TTL gene About this table
Search CenterWatch for drugs/clinical trials and news about TTL
|
Transcripts
for TTL gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for TTL gene: NM_153712.4 Unigene Cluster for TTL: Tubulin tyrosine ligase Hs.358997 [show with all ESTs]Unigene Representative Sequence: BX6491444 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000233336(uc002thu.3) ENST00000485537 ENST00000476615 ENST00000460450
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TTL (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TTL
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): TTL (NM_005371) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TTL | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TTL |
Additional cDNA sequence: AK026673.1 AK126378.1 AK126549.1 BC036331.1 BC036707.2 BC036819.1 BC046913.1 BX538316.1 BX647135.1 BX649144.1 10 DOTS entries: DT.100657812 DT.100748679 DT.217851 DT.101974757 DT.86850481 DT.99929613 DT.100748680 DT.103648 DT.95166168 DT.95262591 24/257 AceView cDNA sequences (see all 257): CR626853 AI580261 AI057587 AI638430 AI073967 T33306 BM985222 BX114610 AA731278 AW498857 AI076216 AL537142 BX282660 CA307361 BQ000802 CR626151 BQ086213 AI075222 BX117060 BQ102470 BC036819 W67381 CR599730 AI655870
GeneLoc Exon Structure
|
Expression
for TTL gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TTL expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CTTTCTAAAA
About this image
See TTL Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TTL
SOURCE GeneReport for Unigene cluster: Hs.358997
SABiosciences Custom PCR Arrays for TTL
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TTL
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat TTL | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TTL | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TTL | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TTL |
Orthologs
for TTL gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for TTL gene from 3/16 species (see all 16) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
TTL1 |
tubulin tyrosine ligase |
79.13(n)
88.86(a) |
|
416711 NM_001199392.1 NP_001186321.1 |
lizard
(Anolis carolinensis) |
Reptilia |
TTL6 |
-- |
86(a) |
1 ↔ 1 |
GL343437.1(42093-71966) |
zebrafish
(Danio rerio) |
Actinopterygii |
Dr.114112 |
Transcribed sequences |
73.77(n) |
|
AL721659.1 |
ENSEMBL Gene Tree for TTL (if available)
TreeFam Gene Tree for TTL (if available) |
Paralogs
for TTL gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for TTL gene
- TTLL102 TTLL42 TTLL32 TTLL12 TTLL52 TTLL112 TTLL92 TTLL72
- TTLL62 TTLL82 TTLL22 TTLL132 ARPC4-TTLL32
TTL for paralogs About GeneDecksing
2 Pseudogenes.org Pseudogenes for TTL
PGOHUM00000240246 PGOHUM00000240857
|
Genomic Variants
for TTL gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 2 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for TTL (113239731 - 113290227 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for TTL: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TTL |
|
Disorders
/ Diseases
for TTL gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database, Novoseek,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TTL for disorders About GeneDecksing
OMIM gene information: 608291
OMIM disorders: --
4  diseases for TTL: About MalaCardsconduct disorder prostate cancer breast cancer prostatitis
2 Novoseek disease relationships for TTL gene About this table
Human Genome Epidemiology (HuGE) Navigator: TTL (1 document)
Export disorders for TTL gene to outside databases
|
Publications
for TTL gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TTL gene, integrated from 9 sources (see all 23):
(articles sorted by number of sources associating them with TTL) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Tubulin detyrosination is a frequent occurrence in breast cancers of poor prognosis. (PubMed id 11431336)1, 3 Mialhe A....Job D. (2001)
- Cloning and genomic organization of the TTL gene on mouse chromosome 2 and human chromosome 2q13. (PubMed id 14571137)1, 9 Erck C....Wehland J. (2003)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- Evidence for genes on chromosome 2 contributing to al cohol dependence with conduct disorder and suicide attempts. (PubMed id 20468071)1 Dick D.M....Bierut L. (2010)
- Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
- The full-ORF clone resource of the German cDNA consortium. (PubMed id 17974005)2 Bechtel S.... Schupp I. (2007)
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621)2 Hillier L.W....Wilson R.K. (2005)
- Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. (PubMed id 15146197)1 Brandenberger R.... Stanton L.W. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for TTL gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing TTL gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TTL gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TTL gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for TTL gene:
Search GeneIP for patents involving TTL
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for TTL gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for TTL | | Browse OriGene shRNA RFPs | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TTL | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TTL | | | OriGene Protein Over-expression Lysate for TTL | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for TTL | | OriGene 3'-UTR Clone for TTL | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TTL | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TTL | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for TTL | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for TTL | | OriGene Custom Protein Services for TTL | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat TTL | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TTL | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TTL | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TTL | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TTL | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TTL |
| |
 |  |  |  | |
| | | | Search Tocris compounds for TTL |
| |  |  |  |  | | | | |
 |
 |
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 | | | TTL Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TTL |
|  |  |  | | | | Search ThermoFisher Antibodies for TTL |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TTL |
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