Free for academic non-profit institutions. Other users need a Commercial license

Aliases for TSSK4 Gene

Aliases for TSSK4 Gene

  • Testis-Specific Serine Kinase 4 2 3
  • TSSK5 3 4 6
  • Serine/Threonine-Protein Kinase 22E 3 4
  • Serine/Threonine Kinase 22E 2 3
  • C14orf20 3 4
  • STK22E 3 4
  • TSK-4 3 4
  • Testis-Specific Serine/Threonine-Protein Kinase 4 3
  • Chromosome 14 Open Reading Frame 20 2
  • Testis-Specific Kinase 4 4
  • EC 4
  • EC 2.7.11 63
  • TSSK-4 4
  • TSK4 3

External Ids for TSSK4 Gene

Previous Symbols for TSSK4 Gene

  • C14orf20
  • STK22E

Summaries for TSSK4 Gene

Entrez Gene Summary for TSSK4 Gene

  • This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

GeneCards Summary for TSSK4 Gene

TSSK4 (Testis-Specific Serine Kinase 4) is a Protein Coding gene. Diseases associated with TSSK4 include spondylosis and spondylolisthesis. Among its related pathways are Sweet Taste Signaling. GO annotations related to this gene include protein serine/threonine kinase activity and magnesium ion binding. An important paralog of this gene is TSSK2.

UniProtKB/Swiss-Prot for TSSK4 Gene

  • May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TSSK4 Gene

Genomics for TSSK4 Gene

Genomic Location for TSSK4 Gene

24,205,547 bp from pter
24,208,362 bp from pter
2,816 bases
Plus strand

Genomic View for TSSK4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for TSSK4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TSSK4 Gene

Regulatory Elements for TSSK4 Gene

Proteins for TSSK4 Gene

  • Protein details for TSSK4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Testis-specific serine/threonine-protein kinase 4
    Protein Accession:
    Secondary Accessions:
    • Q2TA60
    • Q6ZNM2

    Protein attributes for TSSK4 Gene

    328 amino acids
    Molecular mass:
    37454 Da
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for TSSK4 Gene


neXtProt entry for TSSK4 Gene

Proteomics data for TSSK4 Gene at MOPED

Selected DME Specific Peptides for TSSK4 Gene

Post-translational modifications for TSSK4 Gene

Other Protein References for TSSK4 Gene

Domains for TSSK4 Gene

Suggested Antigen Peptide Sequences for TSSK4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 protein kinase domain.:
    • Q6SA08
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.:
    • Q6SA08
genes like me logo Genes that share domains with TSSK4: view

Function for TSSK4 Gene

Molecular function for TSSK4 Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by phosphorylation on Thr-197, potentially by autophosphorylation.
UniProtKB/Swiss-Prot Function: May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.

Enzyme Numbers (IUBMB) for TSSK4 Gene

Gene Ontology (GO) - Molecular Function for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding ISS --
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity ISS --
GO:0004713 protein tyrosine kinase activity --
GO:0005515 protein binding IPI 15964553
genes like me logo Genes that share ontologies with TSSK4: view

No data available for Phenotypes , Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for TSSK4 Gene

Localization for TSSK4 Gene

Subcellular locations from

Jensen Localization Image for TSSK4 Gene COMPARTMENTS Subcellular localization image for TSSK4 gene
Compartment Confidence
cytosol 3
peroxisome 2
extracellular 1
nucleus 1

Gene Ontology (GO) - Cellular Components for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA --
GO:0005737 cytoplasm IBA --
genes like me logo Genes that share ontologies with TSSK4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for TSSK4 Gene

Pathways for TSSK4 Gene

SuperPathways for TSSK4 Gene

Superpath Contained pathways
1 Sweet Taste Signaling
genes like me logo Genes that share pathways with TSSK4: view

Pathways by source for TSSK4 Gene

1 Qiagen pathway for TSSK4 Gene

Gene Ontology (GO) - Biological Process for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation ISS --
GO:0007275 multicellular organismal development IEA --
GO:0007283 spermatogenesis IEA --
GO:0009966 regulation of signal transduction IEA --
GO:0030154 cell differentiation IEA --
genes like me logo Genes that share ontologies with TSSK4: view

Compounds for TSSK4 Gene

(3) HMDB Compounds for TSSK4 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
  • adenosindiphosphorsaeure
  • Magnesium
genes like me logo Genes that share compounds with TSSK4: view

Transcripts for TSSK4 Gene

Unigene Clusters for TSSK4 Gene

Testis-specific serine kinase 4:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for TSSK4 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c · 3d ^ 4a · 4b · 4c
SP1: -
SP2: - -
SP3: -
SP4: -

Relevant External Links for TSSK4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for TSSK4 Gene

mRNA expression in normal human tissues for TSSK4 Gene

mRNA differential expression in normal tissues according to GTEx for TSSK4 Gene

This gene is overexpressed in Testis (12.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for TSSK4 Gene

SOURCE GeneReport for Unigene cluster for TSSK4 Gene Hs.314432

mRNA Expression by UniProt/SwissProt for TSSK4 Gene

Tissue specificity: Expressed only in the testis.
genes like me logo Genes that share expressions with TSSK4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for TSSK4 Gene

Orthologs for TSSK4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for TSSK4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia TSSK4 35
  • 99.31 (n)
  • 98.82 (a)
TSSK4 36
  • 99 (a)
(Bos Taurus)
Mammalia TSSK4 35
  • 89.88 (n)
  • 88.96 (a)
TSSK4 36
  • 89 (a)
(Canis familiaris)
Mammalia TSSK4 35
  • 90.04 (n)
  • 87.28 (a)
TSSK4 36
  • 87 (a)
(Mus musculus)
Mammalia Tssk4 35
  • 88.46 (n)
  • 85.8 (a)
Tssk4 16
Tssk4 36
  • 85 (a)
(Monodelphis domestica)
Mammalia TSSK4 36
  • 79 (a)
(Rattus norvegicus)
Mammalia Tssk4 35
  • 89.84 (n)
  • 89.25 (a)
(Anolis carolinensis)
Reptilia TSSK4 36
  • 74 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008735 35
  • 50.52 (n)
  • 41.41 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG9222 35
  • 54.06 (n)
  • 42.18 (a)
CG9222 36
  • 35 (a)
(Caenorhabditis elegans)
Secernentea F23C8.8 36
  • 27 (a)
W02B12.12 35
  • 45.07 (n)
  • 37.2 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes -- 36
  • 10 (a)
FRK1 36
  • 11 (a)
KIN4 36
  • 11 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 35 (a)
Species with no ortholog for TSSK4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for TSSK4 Gene

Gene Tree for TSSK4 (if available)
Gene Tree for TSSK4 (if available)

Paralogs for TSSK4 Gene

Paralogs for TSSK4 Gene

genes like me logo Genes that share paralogs with TSSK4: view

Variants for TSSK4 Gene

Sequence variations from dbSNP and Humsavar for TSSK4 Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type MAF
rs1242017 -- 24,206,363(-) GCAAA(C/T)GCTAT intron-variant
rs1242018 -- 24,204,864(-) AGAAC(G/T)CTCTA upstream-variant-2KB
rs1242019 -- 24,203,915(-) cacca(C/T)gccta upstream-variant-2KB
rs1242020 -- 24,203,834(-) aggtg(A/G)tctgc upstream-variant-2KB
rs1242021 -- 24,203,666(-) ctccc(A/G)cctca upstream-variant-2KB

Relevant External Links for TSSK4 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for TSSK4 Gene

Disorders for TSSK4 Gene

(3) University of Copenhagen DISEASES for TSSK4 Gene

Relevant External Links for TSSK4

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with TSSK4: view

No data available for UniProtKB/Swiss-Prot for TSSK4 Gene

Publications for TSSK4 Gene

  1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  3. TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway. (PMID: 15964553) Chen X. … Yu L. (Biochem. Biophys. Res. Commun. 2005) 3 4
  4. Mutation screening and association study of the TSSK4 Gene in Chinese infertile men with impaired spermatogenesis. (PMID: 18390560) Su D. … Zhang S. (J. Androl. 2008) 3 48
  5. Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent. (PMID: 20378615) Aston K.I. … Carrell D.T. (Hum. Reprod. 2010) 3 48

Products for TSSK4 Gene

Sources for TSSK4 Gene

Back to Top