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Aliases for TSSK4 Gene

Aliases for TSSK4 Gene

  • Testis Specific Serine Kinase 4 2 3
  • Serine/Threonine-Protein Kinase 22E 3 4
  • Testis-Specific Serine Kinase 4 3 5
  • Serine/Threonine Kinase 22E 2 3
  • C14orf20 3 4
  • STK22E 3 4
  • TSK-4 3 4
  • TSSK5 3 4
  • Chromosome 14 Open Reading Frame 20 2
  • Testis-Specific Kinase 4 4
  • EC 4
  • EC 2.7.11 63
  • TSSK-4 4
  • TSK4 3

External Ids for TSSK4 Gene

Previous HGNC Symbols for TSSK4 Gene

  • C14orf20
  • STK22E

Previous GeneCards Identifiers for TSSK4 Gene

  • GC14P023745
  • GC14P024674
  • GC14P004789

Summaries for TSSK4 Gene

Entrez Gene Summary for TSSK4 Gene

  • This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

GeneCards Summary for TSSK4 Gene

TSSK4 (Testis Specific Serine Kinase 4) is a Protein Coding gene. Diseases associated with TSSK4 include oligospermia. Among its related pathways are Sweet Taste Signaling. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TSSK6.

UniProtKB/Swiss-Prot for TSSK4 Gene

  • May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TSSK4 Gene

Genomics for TSSK4 Gene

Regulatory Elements for TSSK4 Gene

Genomic Location for TSSK4 Gene

24,205,530 bp from pter
24,208,362 bp from pter
2,833 bases
Plus strand

Genomic View for TSSK4 Gene

Genes around TSSK4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TSSK4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TSSK4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TSSK4 Gene

Proteins for TSSK4 Gene

  • Protein details for TSSK4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Testis-specific serine/threonine-protein kinase 4
    Protein Accession:
    Secondary Accessions:
    • Q2TA60
    • Q6ZNM2

    Protein attributes for TSSK4 Gene

    328 amino acids
    Molecular mass:
    37454 Da
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for TSSK4 Gene


neXtProt entry for TSSK4 Gene

Proteomics data for TSSK4 Gene at MOPED

Selected DME Specific Peptides for TSSK4 Gene

Post-translational modifications for TSSK4 Gene

Domains & Families for TSSK4 Gene

Gene Families for TSSK4 Gene

Suggested Antigen Peptide Sequences for TSSK4 Gene

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
genes like me logo Genes that share domains with TSSK4: view

Function for TSSK4 Gene

Molecular function for TSSK4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by phosphorylation on Thr-197, potentially by autophosphorylation.
UniProtKB/Swiss-Prot Function:
May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.

Enzyme Numbers (IUBMB) for TSSK4 Gene

Gene Ontology (GO) - Molecular Function for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding ISS --
GO:0004672 protein kinase activity IEA --
genes like me logo Genes that share ontologies with TSSK4: view
genes like me logo Genes that share phenotypes with TSSK4: view

Animal Model Products

  • Taconic Biosciences Mouse Models for TSSK4

No data available for Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for TSSK4 Gene

Localization for TSSK4 Gene

Subcellular locations from

Jensen Localization Image for TSSK4 Gene COMPARTMENTS Subcellular localization image for TSSK4 gene
Compartment Confidence
cytosol 3
peroxisome 2
extracellular 1
nucleus 1

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Gene Ontology (GO) - Cellular Components for TSSK4 Gene

Pathways & Interactions for TSSK4 Gene

SuperPathways for TSSK4 Gene

Superpath Contained pathways
1 Sweet Taste Signaling
genes like me logo Genes that share pathways with TSSK4: view

Pathways by source for TSSK4 Gene

1 Qiagen pathway for TSSK4 Gene

SIGNOR curated interactions for TSSK4 Gene


Gene Ontology (GO) - Biological Process for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA,ISS --
GO:0007275 multicellular organism development IEA --
GO:0030154 cell differentiation IEA --
genes like me logo Genes that share ontologies with TSSK4: view

Drugs & Compounds for TSSK4 Gene

(2) Drugs for TSSK4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
Magnesium Nutra 0

(1) Additional Compounds for TSSK4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with TSSK4: view

Transcripts for TSSK4 Gene

Unigene Clusters for TSSK4 Gene

Testis-specific serine kinase 4:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for TSSK4 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c · 3d ^ 4a · 4b · 4c
SP1: -
SP2: - -
SP3: -
SP4: -

Relevant External Links for TSSK4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for TSSK4 Gene

mRNA expression in normal human tissues for TSSK4 Gene

mRNA differential expression in normal tissues according to GTEx for TSSK4 Gene

This gene is overexpressed in Testis (x12.4).

Protein differential expression in normal tissues from HIPED for TSSK4 Gene

This gene is overexpressed in CD8 Tcells (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for TSSK4 Gene

SOURCE GeneReport for Unigene cluster for TSSK4 Gene Hs.314432

mRNA Expression by UniProt/SwissProt for TSSK4 Gene

Tissue specificity: Expressed only in the testis.
genes like me logo Genes that share expression patterns with TSSK4: view

Protein tissue co-expression partners for TSSK4 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for TSSK4 Gene

Orthologs for TSSK4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for TSSK4 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia TSSK4 35
  • 89.88 (n)
  • 88.96 (a)
TSSK4 36
  • 89 (a)
(Canis familiaris)
Mammalia TSSK4 35
  • 90.04 (n)
  • 87.28 (a)
TSSK4 36
  • 87 (a)
(Mus musculus)
Mammalia Tssk4 35
  • 88.46 (n)
  • 85.8 (a)
Tssk4 16
Tssk4 36
  • 85 (a)
(Pan troglodytes)
Mammalia TSSK4 35
  • 99.31 (n)
  • 98.82 (a)
TSSK4 36
  • 99 (a)
(Rattus norvegicus)
Mammalia Tssk4 35
  • 89.84 (n)
  • 89.25 (a)
(Monodelphis domestica)
Mammalia TSSK4 36
  • 79 (a)
(Anolis carolinensis)
Reptilia TSSK4 36
  • 74 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008735 35
  • 50.52 (n)
  • 41.41 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG9222 35
  • 54.06 (n)
  • 42.18 (a)
CG9222 36
  • 35 (a)
(Caenorhabditis elegans)
Secernentea W02B12.12 35
  • 45.07 (n)
  • 37.2 (a)
F23C8.8 36
  • 27 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes -- 36
  • 10 (a)
FRK1 36
  • 11 (a)
KIN4 36
  • 11 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 35 (a)
Species with no ortholog for TSSK4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for TSSK4 Gene

Gene Tree for TSSK4 (if available)
Gene Tree for TSSK4 (if available)

Paralogs for TSSK4 Gene

Paralogs for TSSK4 Gene

genes like me logo Genes that share paralogs with TSSK4: view

Variants for TSSK4 Gene

Sequence variations from dbSNP and Humsavar for TSSK4 Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type
rs36036137 - 24,206,020(+) TTGGC(C/T)ATGGC intron-variant, reference, missense
rs34083933 - 24,206,549(+) CTTCT(A/G)TCGGG reference, missense
rs35468205 - 24,206,716(+) GCATC(A/G)TGCAC reference, missense
rs1270764 - 24,207,292(-) AAGTC(C/T)GGCTG reference, missense
rs35244223 - 24,208,139(+) AATTA(C/T)GACCT reference, missense

Variation tolerance for TSSK4 Gene

Residual Variation Intolerance Score: 62.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.22; 69.94% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for TSSK4 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for TSSK4 Gene

Disorders for TSSK4 Gene

MalaCards: The human disease database

(1) MalaCards diseases for TSSK4 Gene - From: DISEASES

Disorder Aliases PubMed IDs
- elite association - COSMIC cancer census association via MalaCards
Search TSSK4 in MalaCards View complete list of genes associated with diseases

Relevant External Links for TSSK4

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with TSSK4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for TSSK4 Gene

Publications for TSSK4 Gene

  1. Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent. (PMID: 20378615) Aston K.I. … Carrell D.T. (Hum. Reprod. 2010) 3 48 67
  2. Testis specific serine/threonine protein kinase 4 (TSSK4) leads to cell apoptosis relying on its kinase activity. (PMID: 25877358) Wang X.L. … Yu L. (J. Huazhong Univ. Sci. Technol. Med. Sci. 2015) 3
  3. Toward an understanding of the protein interaction network of the human liver. (PMID: 21988832) Wang J. … Yang X. (Mol. Syst. Biol. 2011) 3
  4. Mutation screening and association study of the TSSK4 Gene in Chinese infertile men with impaired spermatogenesis. (PMID: 18390560) Su D. … Zhang S. (J. Androl. 2008) 3
  5. DAZL binds to the transcripts of several Tssk genes in germ cells. (PMID: 18452650) Zeng M. … Ma Y. (BMB Rep 2008) 3

Products for TSSK4 Gene

Sources for TSSK4 Gene

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