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TSPY1 Gene

protein-coding   GIFtS: 50

GC0YP009898
testis specific protein, Y-linked 1
(Previous names: testis specific protein, Y-linked )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TSPY)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
CT78 3
DYS14 2
TSPY 2, 3
pJA923 2
Descriptions
Cancer/testis antigen 78 3
testis specific protein, Y-linked 1
testis specific protein, Y-linked 1 2
External Ids
HGNC: 123811
Entrez Gene: 72582
UniProtKB: Q015343
Ensembl: ENSG000001687577
Search outside databases for aliases for TSPY1 gene

Previous GC identifer: GC0YP008919

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534
Function: May be involved in sperm differentiation and proliferation

Gene Wiki entry for TSPY1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TSPY1 gene  

Entrez Gene cytogenetic band: Yp11.2   Ensembl cytogenetic band:  Yp11.2   HGNC cytogenetic band: Yp11.2

TSPY1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome Y         GeneLoc Exon Structure

GeneLoc location for GC0YP009898:     (about GC identifiers)

Start:
9,914,564 bp from pter
End:
9,917,358 bp from pter
Size:
2,795 bases
Orientation:
plus strand

1 alternative location:
ChrY+ 6,174,264-6,177,053     
RefSeq DNA sequence:
NC_000024.8  NT_011878.9  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534 (See protein sequence)
Recommended Name: Testis-specific Y-encoded protein 1  
Size: 308 amino acids; 35101 Da
Subcellular location: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Also found in nucleus
Developmental stage: Detected in 22-week old testis
Sequence caution: Sequence=AAA61238.1; Type=Miscellaneous discrepancy; Note=Probable unspliced
transcript; Sequence=X74029; Type=Frameshift; Positions=Several;
Secondary accessions: O00216 P09002 Q0VAD3 Q9UNN7
Alternative splicing: 2 isoforms:  Q01534-1   Q01534-2   (Additional isoforms seem to exist)

Post-translational modifications:

  • Phosphorylated1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003299.2  

    ENSEMBL proteins: 
    ENSP00000372512 ENSP00000314077 


    Human Recombinant Proteins 
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (TSPY1)
    Human Recombinant Proteins from Abnova (TSPY1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575 cellular_component ND3479749
    GO:0005634 nucleus IEA--
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for TSPY1: 
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    Antibodies from Abcam (TSPY1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (TSPY1)
    Novus Biologicals Antibody for TSPY1

    Assays for TSPY1: 
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    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR002164 NAP_family


       GeneDecks  TSPY1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q01534

    ProtoNet protein and cluster: Q01534

    1 Blocks protein family: IPB002164 Nucleosome assembly protein (NAP)

    UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534
    Similarity: Belongs to the nucleosome assembly protein (NAP) family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Browse Invitrogen for RNAi
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TSPY1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003308

                  Applied Biosystems Silencer® siRNAs for TSPY1

                  Sigma-Aldrich siRNA for TSPY1  
                         Sigma-Aldrich shRNA for TSPY1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003308
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003308
                                     untagged cDNA clone in CMV expression vector: NM_003308 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003308

    UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534
    Function: May be involved in sperm differentiation and proliferation
    Induction: Up-regulated by androgen in a prostate cancer cell line

    1 MGI mutant phenotype (inferred from 2 alleles(MGI details for Tspy-ps):

    muscle

    2 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674 molecular_function ND3479749
    GO:0042802 identical protein binding IPI18649364
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    4 Interacting proteins for TSPY1 (ENSP000003140773 Q015341) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TSPY1Q015341EBI-1973142, EBI-1973142
    Eef1a1 Eef1a1 (xeno)P101261EBI-1973142, EBI-773865
    Eef1a2 Eef1a2 (xeno)P626311EBI-1973142, EBI-642385
    SRYENSP000003725473STRING (score=.708)
    About this table

    5/7 Gene Ontology (GO) biological process terms (links to tree view) (see all 7 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006334 nucleosome assembly IEA--
    GO:0007275 multicellular organismal development IEA--
    GO:0007283 spermatogenesis TAS8923009
    GO:0007506 gonadal mesoderm development IEA--
    GO:0007548 sex differentiation TAS1284595
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for TSPY1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Browse Invitrogen for RNAi
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TSPY1)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003308

                  Sigma-Aldrich siRNA for TSPY1  
                         Sigma-Aldrich shRNA for TSPY1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003308  

    REFSEQ mRNAs for TSPY1 gene: 

    NM_003308.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003308  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003308
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003308
                                     untagged cDNA clone in CMV expression vector: NM_003308 

    Additional cDNA sequence: U58096.1 

    6 DOTS entries:

    DT.410181  DT.120653978  DT.120653195  DT.40106229  DT.120651221  DT.120651912 

    8 AceView cDNA sequences:

    NM_003308 BI828033 AI138810 U58096 AA993340 M94893 NM_022573 BG198129 

    highest scoring ESTs for TSPY1:

    M94893 U58096 NM_003308 AK093413 AA608988 AA725833 AI138810 AY130858 BG198129 M98525 

    Unigene Clusters for TSPY1:

    Testis specific protein, Y-linked 1
    Hs.592352 , Hs.647494  [show with all ESTs], Hs.710329
    Unigene Representative Sequences: AK308271, M94893, U58096


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000383042  ENST00000320701  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    TSPY1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for TSPY1

    1 / 2 / 3

    6 probe-sets matching TSPY1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    35930_at2, 3 U95-A 2 1.00 0.50 1.00 1.00 M94893 1.00 0.85 0.93 1

    35929_s_at2, 3 U95-A 16 1.00 0.11 -0.54 0.51 U58096 1.00 0.65 0.84 1

    217160_at2, 3 U133-A 2 1.00 0.82 -- -- M94893 1.00 0.85 0.93 1

    207918_s_at2, 3 U133-A 12 1.00 0.12 -- -- NM_003308 0.40 1.00 0.76 1

    217160_at2 U133Plus2 2 1.00 0.82 -- -- -- -- -- -- --

    207918_s_at2 U133Plus2 12 1.00 0.12 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene clusters: Hs.592352 Hs.647494 Hs.710329

    Expression variation in blood from EXPOLDB for TSPY1

    UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534
    Tissue specificity: Specifically expressed in testicular tissues. Isoform 1 and isoform 2 are
    expressed in spermatogonia and spermatocytes. Found in early testicular carcinoma in situ,
    spermatogonial cells in testicular tissues of 46,X,Y female and in prostate cancer cell lines

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for TSPY1 gene from 3 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    LOC4733011   -- similar to Testis-Specific Protein Y (TSPY) 81.51(n)
    70.55(a)
    473301  XM_528672.2  XP_528672.2 
    cow
    (Bos taurus)
    LOC7868111   -- similar to Testis-specific Y-encoded protein 1 (Cancer/testis more 69.81(n)
    53.21(a)
    786811  XM_001254382.1  XP_001254383.1 
    mouse
    (Mus musculus)
    Tspy-ps5 Y testis specific protein-Y encoded, pseudogene   -- AJ001379 
    About this table        Species with no ortholog for TSPY1

    ENSEMBL Gene Tree for TSPY1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for TSPY1 gene
    LOC7283951 2  LOC7284121  LOC7281321  LOC7281371  TSPY21  LOC7284031  SET2  TSPYL12  
    TSPYL62  ENSG000001876572  ENSG000002156012  TSPYL22  TSPYL52  TSPYL42  ENSG000001886562  TSPY32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section

    UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534
    Polymorphism: Variants Val-Glu-Val-Val-Ala-Glu-79 Ins and Arg-195 are shown to be present in a
    number of TSPY1 copies of the Yp11 loci. Variant Arg-195 is shown to be present at least in one
    TSPY1 copy of the Yq locus
    Polymorphism: Maps to a tandemly repeated region on chromosome Yp11; additionally at least one copy
    is reported originating from Yq. The gene is thought to be present with an inter-individual
    variation in copy number and between 20 and 60 copies per Y chromosome are expected.
    PubMed:12815422 reports 35 tandemly repeated gene copies on Yp11 originating from one individual

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 480100

    UniProtKB/Swiss-Prot: TSPY1_HUMAN, Q01534
  • May be associated with gonadoblastoma (GBY) [MIM:424500]. GBY is a rare tumor that rises
    mostly in the dysgenetic gonads of phenotypic females who harbor some Y-chromosomal material.
    Copies of TSPY fall within the GBY critical region and TSPY1 is preferentially expressed in the
    germ cells of tumor aggregates in GBY
  • Expression correlates with testicular seminoma, and tumorigenesis of the prostate gland
  • Lies in a chromosomal region that is frequently deleted in infertile males
  • 9 Novoseek disease relationships for TSPY1 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    gonadoblastoma 90.85 18 17349529 (6), 10629567 (2), 15241014 (2), 10090875 (1) (see all 8)
    testicular carcinoma 72.42 1 8923009 (1)
    turners syndrome 55.32 1 18545811 (1)
    prostate cancer 38.00 8 11743352 (2), 16618725 (2), 10090875 (1), 11257712 (1) (see all 6)
    azoospermia 35.09 1 15524154 (1)
    infertility male 32.96 2 17509197 (2)
    tumors 5.62 4 17349529 (1), 8533770 (1), 17977648 (1), 16618725 (1)
    carcinoma 1.37 1 17349529 (1)
    cancer 0.00 2 16106251 (1), 16207477 (1)
    About this table

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/78 PubMed articles for TSPY1 gene (see all 78 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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    Entrez Gene: 7258 HGNC: 12381 AceView: TSPY1.2 Ensembl:ENSG00000168757 euGenes: HUgn7258
    ECgene: TSPY1 H-InvDB: TSPY1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for TSPY1 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TSPY1:
     TaqMan ® Gene Expression Assays
      Free SNP selection tool



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