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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TSPAN8 Gene

protein-coding   GIFtS: 51
GCID: GC12M071518

tetraspanin 8

(Previous name: transmembrane 4 superfamily member 3 )
(Previous symbol: TM4SF3)
 Explore 13 diseases affiliated with
TSPAN8 via our new
 Human Malady Compendium 
Biological research products
for TSPAN8
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tetraspanin 81 2     Tumor-Associated Antigen CO-0292 3
TM4SF31 2 3 5     Tetraspanin-81
Transmembrane 4 Superfamily Member 31 2 3     Tspan-83
CO-0291 2     Tspan-83

External Ids:    HGNC: 118551   Entrez Gene: 71032   Ensembl: ENSG000001273247   OMIM: 6007695   UniProtKB: P190753   

Export aliases for TSPAN8 gene to outside databases

Previous GC identifers: GC12M069806 GC12M068568


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TSPAN8:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family.
Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains.
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation,
growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This
gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene.
(provided by RefSeq, Jul 2008)

Gene Wiki entry for TSPAN8


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_029419.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TSPAN8 gene promoter:
         SRF   TBP   Bach2   SRF (504 AA)   LCR-F1   E47   FAC1   POU2F1   POU2F1a   LyF-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTSPAN8 promoter sequence
   Search SABiosciences Chromatin IP Primers for TSPAN8

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TSPAN8


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q14.1-q21.1   Ensembl cytogenetic band:  12q21.1   HGNC cytogenetic band: 12q14.1-q21.1

TSPAN8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TSPAN8 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M071518:  view genomic region     (about GC identifiers)

Start:
71,518,865 bp from pter      End:
71,835,678 bp from pter
Size:
316,814 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TSN8_HUMAN, P19075 (See protein sequence)
Recommended Name: Tetraspanin-8  
Size: 237 amino acids; 26044 Da
Subcellular location: Membrane; Multi-pass membrane protein
Secondary accessions: B2R7T7 Q9BS78

Explore the universe of human proteins at neXtProt for TSPAN8: NX_P19075

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_P19075

  • TSPAN8 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_004607.1  
    ENSEMBL proteins: 
     ENSP00000377003   ENSP00000247829   ENSP00000447160   ENSP00000449820  

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    Uscn Proteins for TSPAN8

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005764lysosome TAS8661157
    GO:0016021integral to membrane IEA--


    TSPAN8 for ontologies           About GeneDecksing



    TSPAN8 Antibody Products: 
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    Uscn ELISAs and CLIAs for TSPAN8


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TSPAN8 for domains           About GeneDecksing

    4 InterPro domains/families:
     IPR018499 Tetraspanin/Peripherin
     IPR018503 Tetraspanin_CS
     IPR000301 Tetraspanin
     IPR008952 Tetraspanin_EC2

    Graphical View of Domain Structure for InterPro Entry P19075

    ProtoNet protein and cluster: P19075

    1 Blocks protein family: IPB000301 Transmembrane four family signature

    UniProtKB/Swiss-Prot: TSN8_HUMAN, P19075
    Similarity: Belongs to the tetraspanin (TM4SF) family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004871signal transducer activity TAS2395876


    TSPAN8 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TSPAN8

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006486protein glycosylation TAS2395876
    GO:0007165signal transduction TAS2395876
    GO:0030195negative regulation of blood coagulation IEA--


    TSPAN8 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TSPAN8
    Search CenterWatch for drugs/clinical trials and news about TSPAN8 / TSN8 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TSPAN8 gene: 
    NM_004616.2  

    Unigene Cluster for TSPAN8:

    Tetraspanin 8
    Hs.170563  [show with all ESTs]
    Unigene Representative Sequence: BC070168
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000393330(uc001swk.1) ENST00000247829(uc001swj.1) ENST00000546561(uc009zrt.1)
    ENST00000552128 ENST00000552786 ENST00000550818 ENST00000549421

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    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TSPAN8 (see all 7)
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    Clone
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TSPAN8
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    Additional cDNA sequence: 

    AK223022.1 AK313112.1 BC005246.1 BC070168.1 M35252.1 

    11 DOTS entries:

    DT.91761980  DT.100679247  DT.100795484  DT.99958781  DT.97851205  DT.121127945  DT.121127930  DT.100025136 
    DT.40283665  DT.91761979  DT.95118117 

    24/278 AceView cDNA sequences (see all 278):

    BM817951 AI720812 BQ717599 CB111217 AA135381 BQ268328 CB115587 BM834820 
    AW027816 BM850220 BM829765 CB110161 BQ083794 NM_004616 BM789526 BM836533 
    BM820405 CB110798 BQ084137 AA581843 AI075026 BM832339 BM856451 CB158527 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TSPAN8 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CCAAAGCTAT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    TSPAN8 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    4 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    HeartAtrioventricular CanalAtrioventricular Canal CellsMyocardium
    BoneZeugopod Epiphyseal EndBone
    KidneyRenal Collecting Duct SystemKidney
    KidneyS-shaped BodyKidney
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See TSPAN8 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TSPAN8

    SOURCE GeneReport for Unigene cluster: Hs.170563

    UniProtKB/Swiss-Prot: TSN8_HUMAN, P19075
    Tissue specificity: Gastric, colon, rectal, and pancreatic carcinomas

        SABiosciences Custom PCR Arrays for TSPAN8
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TSPAN8

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for TSPAN8 gene from 4/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves TSPAN81 tetraspanin 8 61.99(n)
    56.17(a)
      417854  NM_001199650.1  NP_001186579.1 
    lizard
    (Anolis carolinensis)
    Reptilia TSPAN86
    --
    50(a)
    1 ↔ 1
    5(38175724-38197092)
    African clawed frog
    (Xenopus laevis)
    Amphibia tm4sf3-prov2 transmembrane 4 superfamily member 3 75.35(n)    BC054295.1 
    rainbow trout
    (Oncorhynchus mykiss)
    Actinopterygii Omy.85882 Oncorhynchus mykiss transcribed sequence with weak more 74.71(n)    BX084096.1 


    ENSEMBL Gene Tree for TSPAN8 (if available)
    TreeFam Gene Tree for TSPAN8 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TSPAN8 gene
    CD1512  TSPAN12  CD372  TSPAN62  TSPAN92  TSPAN32  CD632  CD532  
    CD822  TSPAN182  TSPAN72  TSPAN42  TSPAN112  ENSG000002503492  
    18/20 SIMAP similar genes for TSPAN8 using alignment to 2 protein entries:     TSN8_HUMAN (see all proteins) (see all similar genes):
    BTCC-1    CD9    TSPAN2    CD151    TSPAN4    CD81
    TSPAN1    TSPAN18    TM4SF2    TSPAN33    TSPAN7    TSPAN9
    TSPAN6    TSPAN11    CD63    CD53    TSPAN3    CD82

    TSPAN8 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/691 NCBI SNPs in TSPAN8 are shown (see all 691    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1429660001,2
    C,other71531834(+) AATGCA/GATCAG 2 R C mis10--------
    rs603824231,2
    C,--71518392(+) AAAGAG/ACTATA 1 -- ds50012Minor allele frequency- A:0.04WA 120
    rs1131996101,2
    --71518454(+) AAAAAC/TGTTAA 1 -- ds50011Minor allele frequency- T:0.00CSA 1
    rs774218941,2
    C--71518470(+) TCTGAG/TAACTA 1 -- ds50010--------
    rs111786351,2
    C,F,H,--71518710(+) CAATGG/TTTGAT 1 -- ds50016Minor allele frequency- T:0.04NS CSA WA EA 351
    rs798559171,2
    F,--71518744(+) TCTGAA/TAAGCC 1 -- ds50011Minor allele frequency- T:0.03WA 118
    rs1917987631,2
    --71518773(+) CTATCA/TATGGC 1 -- ds50010--------
    rs784413471,2
    F,--71518794(+) TAGAGA/GTGATT 1 -- ds50011Minor allele frequency- G:0.05WA 118
    rs13902071,2
    C,F,O,A,H,--71518841(-) AGGTGG/CTACAG 1 -- ds500124Minor allele frequency- C:0.15EA MN NS NA WA CSA 2741
    rs1466693281,2
    --71518887(+) AAACAA/GTTACT 1 -- ut310--------

    HapMap Linkage Disequilibrium report for TSPAN8 (71518865 - 71768865 bp, first 250kb of TSPAN8)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 7 variations for TSPAN8
         2 CNVs: 76079 86450
         3 Indels: 76080 66264 101556
         2 Inversions: 103328 59712

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TSPAN8 for disorders           About GeneDecksing

    OMIM gene information: 600769    OMIM disorders: --

    13 diseases for TSPAN8:    About MalaCards
    carcinoma    diabetes mellitus    esophageal carcinoma    hepatocellular carcinoma
    esophagitis    pancreatic carcinoma    colorectal cancer    prostate cancer
    pancreatitis    obesity    prostatitis    schizophrenia
    melanoma

    Human Genome Epidemiology (HuGE) Navigator: TSPAN8 (42 documents)

    Export disorders for TSPAN8 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TSPAN8 gene, integrated from 9 sources (see all 49):
    (articles sorted by number of sources associating them with TSPAN8)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning of cDNA for the human tumor-associated antigen CO- 029 and identification of related transmembrane antigens. (PubMed id 2395876)1, 2, 3, 9 Szala S....Linnenbach A.J. (1990)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Whole-genome association study of bipolar disorder. (PubMed id 18317468)1, 9 Sklar P....Purcell S.M. (2008)
    4. Tetraspanin CO-029 inhibits colorectal cancer cell mov ement by deregulating cell-matrix and cell-cell adhesions. (PubMed id 22679508)1 Guo Q....Zhang X.A. (2012)
    5. Gene expression profiles of human melanoma cells with different invasive potential reveal TSPAN8 as a novel mediator of invasion. (PubMed id 21081927)1 Berthier-Vergnes O....Lamartine J. (2011)
    6. Activation-induced internalization differs for the te traspanins CD9 and Tspan8: Impact on tumor cell motility. (PubMed id 20937409)1 Rana S....Zoeller M. (2011)
    7. Evaluating the discriminative power of multi-trait ge netic risk scores for type 2 diabetes in a northern Swedish population. (PubMed id 20571754)1 Fontaine-Bisson B....Franks P.W. (2010)
    8. Gene variants in the novel type 2 diabetes loci CDC12 3/CAMK1D, THADA, ADAMTS9, BCL11A, and MTNR1B affect different aspects of pancre atic beta-cell function. (PubMed id 19833888)1 Simonis-Bik A.M....'t Hart L.M. (2010)
    9. Impact of single nucleotide polymorphisms and of clin ical risk factors on newa89onset diabetes mellitus in HIVa89infected individuals. (PubMed id 20879858)1 Rotger M....Tarr P.E. (2010)
    10. Type 2 diabetes risk alleles near ADCY5, CDKAL1 and H HEX-IDE are associated with reduced birthweight. (PubMed id 20490451)1 Andersson E.A....Pedersen O. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 7103 HGNC: 11855 AceView: TM4SF3 Ensembl:ENSG00000127324 euGenes: HUgn7103
    ECgene: TSPAN8 H-InvDB: TSPAN8

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TSPAN8 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for TSPAN8 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TSPAN8 gene:
    Search GeneIP for patents involving TSPAN8

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
    Advanced Cell Diagnostics
    About This Section

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