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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TSPAN31 Gene

protein-coding   GIFtS: 51
GCID: GC12P058138

tetraspanin 31

(Previous name: sarcoma amplified sequence )
(Previous symbol: SAS)
 Explore 14 diseases affiliated with
TSPAN31 via our new
 Human Malady Compendium 
Biological research products
for TSPAN31
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tetraspanin 311 2     Tetraspanin-311
SAS1 2 3 5     Transmembrane 4 Protein2
Sarcoma Amplified Sequence1 2     Tspan-313
Sarcoma-Amplified Sequence2 3     Tspan-313

External Ids:    HGNC: 105391   Entrez Gene: 63022   Ensembl: ENSG000001354527   OMIM: 1810355   UniProtKB: Q129993   

Export aliases for TSPAN31 gene to outside databases

Previous GC identifers: GC12P056426 GC12P055175


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TSPAN31:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family.
Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains.
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation,
growth and motility. This encoded protein is thought to be involved in growth-related cellular processes. This gene is
associated with tumorigenesis and osteosarcoma. (provided by RefSeq, Jul 2008)

Gene Wiki entry for TSPAN31


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_029419.12  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TSPAN31 gene promoter:
         E2F-3a   c-Fos   AP-1   E2F-2   c-Jun   NF-kappaB   NF-kappaB1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTSPAN31 promoter sequence
   Search SABiosciences Chromatin IP Primers for TSPAN31

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TSPAN31


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.3   Ensembl cytogenetic band:  12q14.1   HGNC cytogenetic band: 12q13-q14

TSPAN31 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TSPAN31 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P058138:  view genomic region     (about GC identifiers)

Start:
58,131,796 bp from pter      End:
58,143,994 bp from pter
Size:
12,199 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TSN31_HUMAN, Q12999 (See protein sequence)
Recommended Name: Tetraspanin-31  
Size: 210 amino acids; 23053 Da
Subcellular location: Membrane; Multi-pass membrane protein
Secondary accessions: O00577 Q53X76

Explore the universe of human proteins at neXtProt for TSPAN31: NX_Q12999

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q12999

  • TSPAN31 Protein expression data from MOPED and PaxDb:    About this image 
    TSPAN31 Protein Expression
    REFSEQ proteins: NP_005972.1  
    ENSEMBL proteins: 
     ENSP00000257910   ENSP00000448209   ENSP00000449312   ENSP00000446482   ENSP00000450195  
     ENSP00000449199   ENSP00000449131  

    Human Recombinant Protein Products for TSPAN31: 
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    Browse ProSpec Recombinant Proteins
    Uscn Proteins for TSPAN31

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005624membrane fraction ----
    GO:0005887integral to plasma membrane TAS8134123
    GO:0016020membrane TAS8134123
    GO:0016021integral to membrane ----

    TSPAN31 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for TSPAN31


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TSPAN31 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR018499 Tetraspanin/Peripherin
     IPR000301 Tetraspanin

    Graphical View of Domain Structure for InterPro Entry Q12999

    ProtoNet protein and cluster: Q12999

    1 Blocks protein family: IPB000301 Transmembrane four family signature

    UniProtKB/Swiss-Prot: TSN31_HUMAN, Q12999
    Similarity: Belongs to the tetraspanin (TM4SF) family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Phenotypes:
         1 MGI phenotypic allele for Tspan31 (no phenotypes)

    TSPAN31 for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for TSPAN31 

    miRNA
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    hsa-miR-548d-3p hsa-miR-2278 hsa-miR-513c hsa-miR-371-5p hsa-miR-373* hsa-miR-33a* hsa-miR-4261 hsa-miR-135b
    SwitchGear 3'UTR luciferase reporter plasmidTSPAN31 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TSPAN31 (see all 7)
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TSPAN31


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TSPAN31

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    3 Interacting proteins for TSPAN31 (Q129993 ENSP000002579104) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    MED6O755863I2D: score=2 
    DDA1ENSP000003529284STRING: ENSP00000352928
    ELAVL1ENSP000003852694STRING: ENSP00000385269
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008284positive regulation of cell proliferation TAS8134123

    TSPAN31 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TSPAN31
    Search CenterWatch for drugs/clinical trials and news about TSPAN31 / TSN31 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TSPAN31 gene: 
    NM_005981.3  

    Unigene Cluster for TSPAN31:

    Tetraspanin 31
    Hs.632708  [show with all ESTs]
    Unigene Representative Sequence: AB209699
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000553221 ENST00000547311 ENST00000550528 ENST00000257910(uc001spt.3 uc009zqb.3 uc010ssa.2)
    ENST00000547992 ENST00000552816 ENST00000553089 ENST00000549052 ENST00000546993
    ENST00000547472 ENST00000548093 ENST00000548167 ENST00000550791 ENST00000546922


    miRNA
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    8/15 QIAGEN miScript miRNA Assays for microRNAs that regulate TSPAN31 (see all 15):
    hsa-miR-548d-3p hsa-miR-2278 hsa-miR-513c hsa-miR-371-5p hsa-miR-373* hsa-miR-33a* hsa-miR-4261 hsa-miR-135b
    SwitchGear 3'UTR luciferase reporter plasmidTSPAN31 3' UTR sequence
    Inhib. RNA
    Products:
         
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TSPAN31 (see all 7)
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    Additional cDNA sequence: 

    AB209699.1 AK294128.1 AK315765.1 BC010377.1 BX647402.1 CR407647.1 U01160.1 

    19 DOTS entries:

    DT.92408932  DT.40113477  DT.445603  DT.100800137  DT.219465  DT.100851448  DT.121198928  DT.121642996 
    DT.121198876  DT.100701711  DT.121198875  DT.95093290  DT.100667381  DT.100701713  DT.100701889  DT.121198723 
    DT.95339116  DT.100851460  DT.121198852 

    24/198 AceView cDNA sequences (see all 198):

    BX416207 BQ072508 CD672750 CR407647 AA301604 CA449236 NM_005981 AA772366 
    BE350825 AI281002 BQ436397 CA394386 U01160 BI822098 CR623983 CK821636 
    BM462177 BM920971 BM544408 AW409777 AU136058 BF037005 AU103105 CA406334 

    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for TSPAN31 (see all 8)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c
    SP1:                    -     -                 -     -     -                 -                           
    SP2:                    -     -                 -     -     -                 -                           
    SP3:                          -                 -     -     -                                             
    SP4:                    -     -                 -     -     -                                             
    SP5:                    -     -                 -                                                         


    ECgene alternative splicing isoforms for TSPAN31

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TSPAN31 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CCAGATGTGT
    TSPAN31 Expression
    About this image
    See TSPAN31 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TSPAN31

    SOURCE GeneReport for Unigene cluster: Hs.632708
        SABiosciences Custom PCR Arrays for TSPAN31

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for TSPAN31 gene from 5/17 species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Tspan311 , 5 tetraspanin 311, 5 86.51(n)1
    88.1(a)1
      10 (74.50 cM)5
    671251  NM_025982.41  NP_080258.11 
     1270673175 
    lizard
    (Anolis carolinensis)
    Reptilia TSPAN316
    --
    71(a)
    1 ↔ 1
    2(66647334-66669461)
    African clawed frog
    (Xenopus laevis)
    Amphibia BC043968.12   -- 75.22(n)    BC043968.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc567102 similar to sarcoma amplified sequence 75.45(n)   393251  AY398411.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Tsp97E6
    Tetraspanin 97E
    39(a)
    1 → many
    3R(22787446-22790547)


    ENSEMBL Gene Tree for TSPAN31 (if available)
    TreeFam Gene Tree for TSPAN31 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TSPAN31 gene
    TSPAN132  
    2 SIMAP similar genes for TSPAN31 using alignment to 8 protein entries:     TSN31_HUMAN (see all proteins):
    TM4SF13    TSPAN13

    TSPAN31 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/78 NCBI SNPs in TSPAN31 are shown (see all 78    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs576977391,2
    C--55173774(+) GGTTAT/CAATTT 1 -- us2k12Minor allele frequency- C:0.03WA 120
    rs25186881,2
    C--55174404(+) TAAATA/GCTGTG 1 -- us2k12Minor allele frequency- G:0.00NA 4
    rs1917356021,2
    --58136946(+) ATACCC/TTTTTC 2 -- us2k10--------
    rs1398969991,2
    --58137139(+) GGGTAA/GTTTGA 2 -- us2k10--------
    rs1842833161,2
    --58137237(+) CACCAC/GCCTGG 2 -- us2k10--------
    rs1444941181,2
    --58137337(+) CTGAGC/TCAGGC 2 -- us2k10--------
    rs1456604111,2
    --58137460(+) ATAACG/TTAATT 2 -- us2k10--------
    rs1488757171,2
    --58137671(+) GGTACC/TGTTCT 2 -- us2k10--------
    rs619357441,2
    C--58137968(+) CGTGAG/AAAGGA 1 -- us2k11Minor allele frequency- A:0.50NA 2
    rs745655081,2
    C--58138069(+) TAAATG/AAGACT 1 -- us2k12Minor allele frequency- A:0.07CSA WA 120

    HapMap Linkage Disequilibrium report for TSPAN31 (58131796 - 58143994 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 1 variation for TSPAN31
         1 CNV: 58587

    SABiosciences Cancer Mutation PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for TSPAN31

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TSPAN31 for disorders           About GeneDecksing

    OMIM gene information: 181035    OMIM disorders: --

    14 diseases for TSPAN31:    About MalaCards
    osteosarcoma    fibrous histiocytoma    sarcoma    malignant fibrous histiocytoma
    histiocytoma    embryonal rhabdomyosarcoma    chondroma    lipoma
    rhabdomyosarcoma    liposarcoma    leiomyosarcoma    hepatitis b
    hepatitis    neuroblastoma

    3 diseases from the University of Copenhagen DISEASES database for TSPAN31:
    Liposarcoma     Malignant fibroxanthoma     Chondroma
    Human Genome Epidemiology (HuGE) Navigator: TSPAN31 (4 documents)

    Export disorders for TSPAN31 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TSPAN31 gene, integrated from 9 sources (see all 20):
    (articles sorted by number of sources associating them with TSPAN31)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. Transcript mapping in a 46-kb sequenced region at the core of 12q13.3 amplification in human cancers. (PubMed id 9192850)1, 2 Elkahloun A.G....Meltzer P.S. (1997)
    4. SAS, a gene amplified in human sarcomas, encodes a new member of the transmembrane 4 superfamily of proteins. (PubMed id 8134123)1, 2 Jankowski S.A.... Meltzer P.S. (1994)
    5. Amplification of CDK4, MDM2, SAS and GLI genes in leiomyosarcoma, alveolar and embryonal rhabdomyosarcoma. (PubMed id 15024701)1, 9 Ragazzini P....Benassi M.S. (2004)
    6. New genetic associations detected in a host response study to hepatitis B vaccine. (PubMed id 20237496)1 Davila S....Seielstad M. (2010)
    7. Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1 Bailey S.D....Anand S. (2010)
    8. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
    9. Gene-centric association signals for lipids and apoli poproteins identified via the HumanCVD BeadChip. (PubMed id 19913121)1 Talmud P.J.... . (2009)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 6302 HGNC: 10539 AceView: SAS Ensembl:ENSG00000135452 euGenes: HUgn6302
    ECgene: TSPAN31 H-InvDB: TSPAN31

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TSPAN31 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for TSPAN31 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TSPAN31 gene:
    Search GeneIP for patents involving TSPAN31

    GeneCards and IP:
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    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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