TRIM69 Gene
protein-coding GIFtS: 48
GCID: GC15P045027
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tripartite motif containing 69(Previous names: ring finger protein 36, tripartite motif-containing 69...) (Previous symbol: RNF36)
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Aliases for TRIM69 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Tripartite Motif Containing 691 2 | | TRIMLESS1 | | RING Finger Protein 361 2 3 | | HSD342 | | RNF361 2 3 | | E3 Ubiquitin-Protein Ligase TRIM692 | | Trif1 2 | | Trimless1 | | Tripartite Motif-Containing 691 2 | | Tripartite Motif Protein 692 | | Tripartite Motif-Containing Protein 692 3 | | EC 6.3.2.-3 | | RFP-Like Domain-Containing Protein Trimless2 3 | | |
Export aliases for TRIM69 gene to outside databasesPrevious GC identifers: GC15P042815 GC15P045028 GC15P021852 GC15P045025 |
Summaries for TRIM69 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| Entrez Gene summary for TRIM69: This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RINGfinger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed ingerm cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.Alternatively spliced transcript variants encoding distinct isoforms have been described. (provided by RefSeq, Jul2008) UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6Function: May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis
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Genomic Views for TRIM69 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000015.9 NC_018926.1 NT_010194.17
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the TRIM69 gene promoter: AML1a RelA NF-kappaB E47 GATA-1 GATA-6 c-Myb Hand1 TGIF NF-kappaB1 Other transcription factors
Search SABiosciences Chromatin IP Primers for TRIM69
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM69 |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 15q21.1 Ensembl cytogenetic band: 15q21.1 HGNC cytogenetic band: 15q15-q21TRIM69 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 15 GeneLoc Exon Structure GeneLoc location for GC15P045027: view genomic region
(about GC identifiers)
Start:
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45,021,186 bp from pter |
End:
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45,060,027 bp from pter |
Size:
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38,842 bases |
Orientation:
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plus strand |
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Proteins for TRIM69 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6 (See
protein sequence)Recommended Name: E3 ubiquitin-protein ligase TRIM69 Size: 500 amino acids; 57419 Da
Subunit: Interacts with PML (By similarity)
Subcellular location: Cytoplasm. Nucleus. Nucleus speckle
Secondary accessions: A8MX03 Q309B0 Q4G1A5 Q6W897 Q8IYY3 Q8WY16 Q8WY17Alternative splicing: 4 isoforms: Q86WT6-1 Q86WT6-2 Q86WT6-3 Q86WT6-4 Explore the universe of human proteins at neXtProt for TRIM69: NX_Q86WT6
Post-translational modifications:
Phosphorylated. Phosphorylation is necessary for nuclear localization (By similarity)1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q86WT6 TRIM69 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_542783.2 NP_892030.3 ENSEMBL proteins: ENSP00000453177 ENSP00000453549 ENSP00000453332 ENSP00000453077 ENSP00000342922 ENSP00000452855 ENSP00000452739 ENSP00000332284 Human Recombinant Protein Products:
Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table
TRIM69 for ontologies About GeneDecksing
TRIM69 Antibody Products: Assay Products for TRIM69: |
Protein
Domains / Families for TRIM69 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TRIM69 for domains About GeneDecksing
5/10 InterPro domains/families (see all 10):Graphical View of Domain Structure for InterPro Entry Q86WT6ProtoNet protein and cluster: Q86WT6 3 Blocks protein families: IPB001841 Zn-finger IPB003879 Butyrophylin C-terminal DUF signature IPB006574 SPRY-associated domain
UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6Domain: The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localisationand aggregationSimilarity: Belongs to the TRIM/RBCC familySimilarity: Contains 1 B30.2/SPRY domainSimilarity: Contains 1 RING-type zinc finger |
Function for TRIM69 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6Function: May have E3 ubiquitin-protein ligase activity. May play a role in apoptosisEnzyme Number (IUBMB): EC 6.3.2.-1
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM69 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM69 (see all 3) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): TRIM69 (NM_182985) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TRIM69 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM69  |
In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM69 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0004842 | ubiquitin-protein ligase activity |
IDA | -- | | GO:0008270 | zinc ion binding |
IEA | -- |
TRIM69 for ontologies About GeneDecksing
|
Pathways & Interactions for TRIM69 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TRIM69
STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)
 5/7 Interacting proteins for TRIM69 (Q86WT62, 3 ENSP000003322844) via UniProtKB, MINT, STRING, and/or I2D (see all 7) About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006915 | apoptotic process |
IEA | -- |
TRIM69 for ontologies About GeneDecksing
|
Drugs & Compounds for TRIM69 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Browse Tocris compounds for TRIM69 Search CenterWatch for drugs/clinical trials and news about TRIM69 / TRI69 
|
Transcripts for TRIM69 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for TRIM69 gene (2 alternative transcripts): NM_080745.3 NM_182985.3 Unigene Clusters for TRIM69: Tripartite motif containing 69 Hs.489254 [show with all ESTs], Hs.744193 [show with all ESTs]Unigene Representative Sequences: AK226115, BC03126612 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000559390(uc001zuf.2 uc001zug.1 uc001zuh.1 uc001zui.1 uc010bdy.1) ENST00000560141(uc001zue.3) ENST00000560554 ENST00000559315 ENST00000561237 ENST00000560442 ENST00000558329 ENST00000561043 ENST00000338264 ENST00000558173 ENST00000561170 ENST00000329464
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM69 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM69 (see all 3) OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector (see all 2): TRIM69 (NM_182985) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TRIM69 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM69  |
Additional cDNA sequence: AK311550.1 BC031266.1 9 DOTS entries: DT.113579 DT.92048039 DT.105473 DT.121080066 DT.95269722 DT.101968126 DT.91713472 DT.97829242 DT.99957328 24/5611 AceView cDNA sequences (see all 5611): AA373474 BM704605 BM312292 BM768984 R21588 AI273802 AA574169 BU785920 BG252080 AI758718 BE907091 BE172648 BM989633 BU947944 BM846129 BM830272 BU659835 BG106673 CA942899 BM762970 BM773372 BM851697 AA704107 AA769696 GeneLoc Exon Structure
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Expression for TRIM69 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TRIM69 expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: CACTCCTACA
About this image See TRIM69 Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for TRIM69
SOURCE GeneReport for Unigene clusters: Hs.489254 Hs.744193 SABiosciences Custom PCR Arrays for TRIM69
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM69 Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat TRIM69 | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TRIM69 | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TRIM69 | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM69 |
Orthologs for TRIM69 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for TRIM69 gene from 4/10 species (see all 10) About this table
ENSEMBL Gene Tree for TRIM69 (if available) TreeFam Gene Tree for TRIM69 (if available)  |
Paralogs for TRIM69 gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for TRIM69 gene
- TRIM502 TRIM312 TRIM732 TRIM742 TRIM522 TRIM352 TRIM412 TRIM622
- TRIM722
18/24 SIMAP similar genes for TRIM69 using alignment to 3 protein entries: TRI69_HUMAN (see all proteins)
(see all similar genes):ERMAP BTN2A2 MEFV TRIM27 TRIM41 BTN3A3 BT3.3 TRIM21 TRIM39 BTNL8 TRIM15 TRIM68 TRIM26 TRIM62 TRIM38 TRIM60 BTNL9 TRIM10
TRIM69 for paralogs About GeneDecksing
|
Genomic Variants for TRIM69 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 15 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
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HapMap Linkage Disequilibrium report for TRIM69 (45021186 - 45060027 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 2 variations for TRIM69 2 CNVs: 3961 58722  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TRIM69 |
|
Disorders
/ Diseases for TRIM69 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TRIM69 for disorders About MalaCards
TRIM69 for disorders About GeneDecksing
Human Genome Epidemiology (HuGE) Navigator: TRIM69 (2 documents) Export disorders for TRIM69 gene to outside databases
|
Publications for TRIM69 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TRIM69 gene, integrated from 9 sources (see all 15): (articles sorted by number of sources associating them with TRIM69) | |  | Utopia: connect your pdf to the dynamic world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Forced expression of RNF36 induces cell apoptosis. (PubMed id 12837286)1, 9 Shyu H.W....Li H. (2003)
- Characterisation of human RING finger protein TRIM69, a novel testis E3 ubiquitin ligase and its subcellular localisation. (PubMed id 23131556)2 Han Y.... Wang L. (2012)
- E3 ubiquitin ligase CHIP facilitates Toll-like recept or signaling by recruiting and polyubiquitinating Src and atypical PKC{zeta}. (PubMed id 21911421)1 Yang M....Chen T. (2011)
- Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
- An approach based on a genome-wide association study reveals candidate loci for narcolepsy. (PubMed id 20677014)1 Shimada M....Tokunaga K. (2010)
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
- Systematic analysis of human protein complexes identi fies chromosome segregation proteins. (PubMed id 20360068)1 Hutchins J.R....Peters J.M. (2010)
- Analysis of the DNA sequence and duplication history of human chromosome 15. (PubMed id 16572171)2 Zody M.C....Nusbaum C. (2006)
- Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)1 Rual J.F....Vidal M. (2005)
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External Searches for TRIM69 gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
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Genome Databases showing TRIM69 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TRIM69 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TRIM69 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for TRIM69 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for TRIM69 gene: Search GeneIP for patents involving TRIM69
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for TRIM69 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
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 | |
 | |
 |
| | | | OriGene Antibodies for TRIM69 | | OriGene shRNA RFP for TRIM69 | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TRIM69 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM69 | | OriGene Protein Over-expression Lysate for TRIM69 | | Browse OriGene Fluorogenic Cell Assay Kits | | OriGene siRNA for TRIM69 | | OriGene 3'-UTR Clone for TRIM69 | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM69 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM69 | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | OriGene Purified Protein for TRIM69 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | OriGene Custom Antibody Services for TRIM69 | | OriGene Custom Protein Services for TRIM69 | | OriGene Custom Immunoassay Development | | |
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| | | Search Tocris compounds for TRIM69 |
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 | | TRIM69 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM69 |
|  |  |  | | | ThermoFisher Antibodies for TRIM69 |
| | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM69 |
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