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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TRIM69 Gene

protein-coding   GIFtS: 48
GCID: GC15P045027

tripartite motif containing 69

(Previous names: ring finger protein 36, tripartite motif-containing 69...)
(Previous symbol: RNF36)
  Search for TRIM69
in our new
 Human Malady Compendium 
Biological research products
for TRIM69
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tripartite Motif Containing 691 2     TRIMLESS1
RING Finger Protein 361 2 3     HSD342
RNF361 2 3     E3 Ubiquitin-Protein Ligase TRIM692
Trif1 2     Trimless1
Tripartite Motif-Containing 691 2     Tripartite Motif Protein 692
Tripartite Motif-Containing Protein 692 3     EC 6.3.2.-3
RFP-Like Domain-Containing Protein Trimless2 3     

External Ids:    HGNC: 178571   Entrez Gene: 1406912   Ensembl: ENSG000001858807   UniProtKB: Q86WT63   

Export aliases for TRIM69 gene to outside databases

Previous GC identifers: GC15P042815 GC15P045028 GC15P021852 GC15P045025


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TRIM69:
This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING
finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in
germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.
Alternatively spliced transcript variants encoding distinct isoforms have been described. (provided by RefSeq, Jul
2008)

UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6
Function: May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000015.9  NC_018926.1  NT_010194.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TRIM69 gene promoter:
         AML1a   RelA   NF-kappaB   E47   GATA-1   GATA-6   c-Myb   Hand1   TGIF   NF-kappaB1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTRIM69 promoter sequence
   Search SABiosciences Chromatin IP Primers for TRIM69

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM69


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q21.1   Ensembl cytogenetic band:  15q21.1   HGNC cytogenetic band: 15q15-q21

TRIM69 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TRIM69 gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15P045027:  view genomic region     (about GC identifiers)

Start:
45,021,186 bp from pter      End:
45,060,027 bp from pter
Size:
38,842 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6 (See protein sequence)
Recommended Name: E3 ubiquitin-protein ligase TRIM69  
Size: 500 amino acids; 57419 Da
Subunit: Interacts with PML (By similarity)
Subcellular location: Cytoplasm. Nucleus. Nucleus speckle
Secondary accessions: A8MX03 Q309B0 Q4G1A5 Q6W897 Q8IYY3 Q8WY16 Q8WY17
Alternative splicing: 4 isoforms:  Q86WT6-1   Q86WT6-2   Q86WT6-3   Q86WT6-4   

Explore the universe of human proteins at neXtProt for TRIM69: NX_Q86WT6

Post-translational modifications:

  • Phosphorylated. Phosphorylation is necessary for nuclear localization (By similarity)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q86WT6

  • TRIM69 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_542783.2  NP_892030.3  

    ENSEMBL proteins: 
     ENSP00000453177   ENSP00000453549   ENSP00000453332   ENSP00000453077   ENSP00000342922  
     ENSP00000452855   ENSP00000452739   ENSP00000332284  

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    Novus Biologicals TRIM69 Proteins
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    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Uscn Proteins for TRIM69

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005737cytoplasm IDA--
    GO:0016607nuclear speck IDA--


    TRIM69 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TRIM69 for domains           About GeneDecksing

    5/10 InterPro domains/families (see all 10):
     IPR006574 PRY
     IPR017907 Znf_RING_CS
     IPR001841 Znf_RING
     IPR001870 B30.2/SPRY
     IPR003877 SPRY_rcpt

    Graphical View of Domain Structure for InterPro Entry Q86WT6

    ProtoNet protein and cluster: Q86WT6

    3 Blocks protein families:
    IPB001841 Zn-finger
    IPB003879 Butyrophylin C-terminal DUF signature
    IPB006574 SPRY-associated domain


    UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6
    Domain: The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localisation
    and aggregation
    Similarity: Belongs to the TRIM/RBCC family
    Similarity: Contains 1 B30.2/SPRY domain
    Similarity: Contains 1 RING-type zinc finger


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6
    Function: May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis

    Enzyme Number (IUBMB): EC 6.3.2.-1

    miRNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842ubiquitin-protein ligase activity IDA--
    GO:0008270zinc ion binding IEA--


    TRIM69 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    UniProtKB/Swiss-Prot: TRI69_HUMAN, Q86WT6
    Pathway: Protein modification; protein ubiquitination

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TRIM69

    STRING Interaction Network Preview (showing 5 interactants - click image to see more details)

    5/7 Interacting proteins for TRIM69 (Q86WT62, 3 ENSP000003322844) via UniProtKB, MINT, STRING, and/or I2D (see all 7)

    InteractantInteraction Details
    GeneCardExternal ID(s)
    MAGEA4P433582, 3, ENSP000002763444MINT-67646 I2D: score=5 STRING: ENSP00000276344
    PMLP295903, ENSP000002680584I2D: score=2 STRING: ENSP00000268058
    TLR3O154553, ENSP000002967954I2D: score=1 STRING: ENSP00000296795
    TLR4O002063, ENSP000003630894I2D: score=1 STRING: ENSP00000363089
    TLR9Q9NR963, ENSP000003538744I2D: score=1 STRING: ENSP00000353874
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006915apoptotic process IEA--


    TRIM69 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TRIM69
    Search CenterWatch for drugs/clinical trials and news about TRIM69 / TRI69 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TRIM69 gene (2 alternative transcripts): 
    NM_080745.3  NM_182985.3  

    Unigene Clusters for TRIM69:

    Tripartite motif containing 69
    Hs.489254  [show with all ESTs], Hs.744193  [show with all ESTs]
    Unigene Representative Sequences: AK226115, BC031266
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000559390(uc001zuf.2 uc001zug.1 uc001zuh.1 uc001zui.1 uc010bdy.1)
    ENST00000560141(uc001zue.3) ENST00000560554 ENST00000559315 ENST00000561237
    ENST00000560442 ENST00000558329 ENST00000561043 ENST00000338264 ENST00000558173
    ENST00000561170 ENST00000329464

    miRNA
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    Inhib. RNA
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    Additional cDNA sequence: 

    AK311550.1 BC031266.1 

    9 DOTS entries:

    DT.113579  DT.92048039  DT.105473  DT.121080066  DT.95269722  DT.101968126  DT.91713472  DT.97829242 
    DT.99957328 

    24/5611 AceView cDNA sequences (see all 5611):

    AA373474 BM704605 BM312292 BM768984 R21588 AI273802 AA574169 BU785920 
    BG252080 AI758718 BE907091 BE172648 BM989633 BU947944 BM846129 BM830272 
    BU659835 BG106673 CA942899 BM762970 BM773372 BM851697 AA704107 AA769696 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TRIM69 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CACTCCTACA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See TRIM69 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TRIM69

    SOURCE GeneReport for Unigene clusters: Hs.489254 Hs.744193
        SABiosciences Custom PCR Arrays for TRIM69
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM69

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for TRIM69 gene from 4/10 species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves TRIM691 tripartite motif containing 69 48.1(n)
    36.63(a)
      427640  XM_425213.3  XP_425213.3 
    lizard
    (Anolis carolinensis)
    Reptilia TRIM696
    --
    --
    52(a)
    38(a)
    1 ↔ 1
    possible ortholog
    GL343490.1(353796-359149)
    GL343220.1(1692978-1696563)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia 1377116702   -- 70.73(n)    137711670 
    zebrafish
    (Danio rerio)
    Actinopterygii CU929225.16
    --
    27(a)
    1 ↔ 1
    10(42202487-42217782)


    ENSEMBL Gene Tree for TRIM69 (if available)
    TreeFam Gene Tree for TRIM69 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TRIM69 gene
    TRIM502  TRIM312  TRIM732  TRIM742  TRIM522  TRIM352  TRIM412  TRIM622  
    TRIM722  
    18/24 SIMAP similar genes for TRIM69 using alignment to 3 protein entries:     TRI69_HUMAN (see all proteins) (see all similar genes):
    ERMAP    BTN2A2    MEFV    TRIM27    TRIM41    BTN3A3
    BT3.3    TRIM21    TRIM39    BTNL8    TRIM15    TRIM68
    TRIM26    TRIM62    TRIM38    TRIM60    BTNL9    TRIM10

    TRIM69 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/632 NCBI SNPs in TRIM69 are shown (see all 632    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1385555081,2
    --45026619(+) AGGGTA/CTCGCC 2 -- us2k10--------
    rs1140487861,2
    F,--45026869(+) GACACC/TCATAG 2 -- us2k11Minor allele frequency- T:0.03WA 118
    rs49239931,2
    C,F,A,H,--45026900(+) GGCTAC/TTCAGT 2 -- us2k136Minor allele frequency- T:0.10NS EA NA WA CSA 3407
    rs352772591,2
    C,--45026910(+) TGCTCTGG/-TGGTG 2 -- us2k11Minor allele frequency- -:0.50CSA 2
    rs570553361,2
    C--45026912(+) TGGTG-/GTGAGAGA 2 -- us2k11Minor allele frequency- GTG:0.00NA 2
    rs1908643651,2
    --45026951(+) GTACTC/TTTACC 2 -- us2k10--------
    rs1427512061,2
    --45026978(+) AAAAAC/TGTGCA 2 -- us2k10--------
    rs349674371,2
    C,--45027043(+) CAAAA-/ACAATC 2 -- us2k12Minor allele frequency- A:0.25NA CSA 4
    rs1469387641,2
    --45027156(+) AAGGTC/GAGCAA 2 -- us2k10--------
    rs1378919511,2
    --45027183(+) TCCTGC/TGGTTT 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for TRIM69 (45021186 - 45060027 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for TRIM69
         2 CNVs: 3961 58722

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TRIM69 for disorders           About MalaCards

    TRIM69 for disorders           About GeneDecksing

    Human Genome Epidemiology (HuGE) Navigator: TRIM69 (2 documents)

    Export disorders for TRIM69 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TRIM69 gene, integrated from 9 sources (see all 15):
    (articles sorted by number of sources associating them with TRIM69)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    2. Forced expression of RNF36 induces cell apoptosis. (PubMed id 12837286)1, 9 Shyu H.W....Li H. (2003)
    3. Characterisation of human RING finger protein TRIM69, a novel testis E3 ubiquitin ligase and its subcellular localisation. (PubMed id 23131556)2 Han Y.... Wang L. (2012)
    4. E3 ubiquitin ligase CHIP facilitates Toll-like recept or signaling by recruiting and polyubiquitinating Src and atypical PKC{zeta}. (PubMed id 21911421)1 Yang M....Chen T. (2011)
    5. Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
    6. An approach based on a genome-wide association study reveals candidate loci for narcolepsy. (PubMed id 20677014)1 Shimada M....Tokunaga K. (2010)
    7. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1 Rose J.E....Uhl G.R. (2010)
    8. Systematic analysis of human protein complexes identi fies chromosome segregation proteins. (PubMed id 20360068)1 Hutchins J.R....Peters J.M. (2010)
    9. Analysis of the DNA sequence and duplication history of human chromosome 15. (PubMed id 16572171)2 Zody M.C....Nusbaum C. (2006)
    10. Towards a proteome-scale map of the human protein-protein interaction network. (PubMed id 16189514)1 Rual J.F....Vidal M. (2005)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 140691 HGNC: 17857 AceView: B2MandRNF36andLOC400368 Ensembl:ENSG00000185880 euGenes: HUgn140691
    ECgene: TRIM69 H-InvDB: TRIM69

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TRIM69 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TRIM69 gene:
    Search GeneIP for patents involving TRIM69

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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