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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TRIM68 Gene

protein-coding   GIFtS: 49
GCID: GC11M004577

tripartite motif containing 68

(Previous names: ring finger protein 137, tripartite motif-containing 68...)
(Previous symbol: RNF137)
 Explore 6 diseases affiliated with
TRIM68 via our new
 Human Malady Compendium 
Biological research products
for TRIM68
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tripartite Motif Containing 681 2     Tripartite Motif-Containing Protein 682 3
RING Finger Protein 1371 2 3     SSA Protein SS-562 3
RNF1371 2 3     FLJ103691
SS-561 2 3     E3 Ubiquitin-Protein Ligase TRIM682
GC1092 3 5     Ro/SSA1 Related Protein2
SS562 3 5     EC 6.3.2.-3
Tripartite Motif-Containing 681 2     

External Ids:    HGNC: 211611   Entrez Gene: 551282   Ensembl: ENSG000001673337   OMIM: 6131845   UniProtKB: Q6AZZ13   

Export aliases for TRIM68 gene to outside databases

Previous GC identifer: GC11M004278


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TRIM68:
This gene encodes a protein which contains a RING finger domain, a motif present in a variety of functionally distinct
proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many
cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was
also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.
(provided by RefSeq, Oct 2011)

UniProtKB/Swiss-Prot: TRI68_HUMAN, Q6AZZ1
Function: Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its
ubiquitin ligase activity

Gene Wiki entry for TRIM68


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000011.9  NC_018922.1  NT_009237.18  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TRIM68 gene promoter:
         GR   NF-1   NF-1/L   GR-beta   MyoD   GR-alpha   AP-4   NRSF form 2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTRIM68 promoter sequence
   Search SABiosciences Chromatin IP Primers for TRIM68

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM68


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p15.4   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15.4

TRIM68 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TRIM68 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11M004577:  view genomic region     (about GC identifiers)

Start:
4,619,902 bp from pter      End:
4,629,489 bp from pter
Size:
9,588 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TRI68_HUMAN, Q6AZZ1 (See protein sequence)
Recommended Name: E3 ubiquitin-protein ligase TRIM68  
Size: 485 amino acids; 56259 Da
Subunit: Interacts with AR/androgen receptor (via ligand-binding domain). Interacts with KAT5/TIP60
Subcellular location: Cytoplasm, perinuclear region. Nucleus. Note=Colocalized with AR in nucleus
Miscellaneous: Antibodies against TRIM68 are found in patients with systemic lupus erythematosus (SLE) and primary
Sjoegren syndrome
Sequence caution: Sequence=BAA91569.1; Type=Erroneous initiation; Sequence=BAB14309.1; Type=Erroneous initiation;
Secondary accessions: A6NI19 A8K551 B3KPM5 Q8WZ70 Q96LE5 Q96PF7 Q9H9C2 Q9NW18

Explore the universe of human proteins at neXtProt for TRIM68: NX_Q6AZZ1

Post-translational modifications:

  • Auto-ubiquitinated1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q6AZZ1

  • TRIM68 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_060543.5  
    ENSEMBL proteins: 
     ENSP00000300747   ENSP00000431783   ENSP00000434681   ENSP00000436112  

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    Uscn Proteins for TRIM68

    Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IDA--
    GO:0005730nucleolus IDA--
    GO:0005737cytoplasm IDA--
    GO:0005794Golgi apparatus IDA--


    TRIM68 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TRIM68 for domains           About GeneDecksing

    5/10 InterPro domains/families (see all 10):
     IPR006574 PRY
     IPR017907 Znf_RING_CS
     IPR001841 Znf_RING
     IPR001870 B30.2/SPRY
     IPR003877 SPRY_rcpt

    Graphical View of Domain Structure for InterPro Entry Q6AZZ1

    ProtoNet protein and cluster: Q6AZZ1

    4 Blocks protein families:
    IPB000315 B-box zinc finger signature
    IPB001841 Zn-finger
    IPB003879 Butyrophylin C-terminal DUF signature
    IPB006574 SPRY-associated domain


    UniProtKB/Swiss-Prot: TRI68_HUMAN, Q6AZZ1
    Domain: The RING domain is essential for ubiquitin E3 ligase activity
    Similarity: Belongs to the TRIM/RBCC family
    Similarity: Contains 1 B box-type zinc finger
    Similarity: Contains 1 B30.2/SPRY domain
    Similarity: Contains 1 RING-type zinc finger


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: TRI68_HUMAN, Q6AZZ1
    Function: Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its
    ubiquitin ligase activity
    Induction: Up-regulated in prostate cancer

    Enzyme Number (IUBMB): EC 6.3.2.-1

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    hsa-miR-24 hsa-miR-3647-3p hsa-miR-4267
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    Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004842ubiquitin-protein ligase activity IDA18451177
    GO:0008270zinc ion binding IEA--
    GO:0035035histone acetyltransferase binding IPI18451177
    GO:0050681androgen receptor binding IPI18451177


    TRIM68 for ontologies           About GeneDecksing


    3 GenomeRNAi human phenotypes for TRIM68:
     Increased G1 DNA content  Increased HPV18 LCR reporter a  Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    UniProtKB/Swiss-Prot: TRI68_HUMAN, Q6AZZ1
    Pathway: Protein modification; protein ubiquitination

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TRIM68

    STRING Interaction Network Preview (showing 4 interactants - click image to see more details)

    4 Interacting proteins for TRIM68 (Q6AZZ13 ENSP000003007474) via UniProtKB, MINT, STRING, and/or I2D

    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBE2UQ5VVX93, ENSP000003601164I2D: score=2 STRING: ENSP00000360116
    ARP102753, ENSP000003638224I2D: score=1 STRING: ENSP00000363822
    KAT5Q929933, ENSP000003403304I2D: score=1 STRING: ENSP00000340330
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0051865protein autoubiquitination IDA18451177
    GO:0060765regulation of androgen receptor signaling pathway IDA18451177


    TRIM68 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TRIM68
    Search CenterWatch for drugs/clinical trials and news about TRIM68 / TRI68 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TRIM68 gene: 
    NM_018073.6  

    Unigene Cluster for TRIM68:

    Tripartite motif containing 68
    Hs.523438  [show with all ESTs]
    Unigene Representative Sequence: NM_018073
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000300747(uc001lzf.2 uc010qyj.2) ENST00000531101 ENST00000526337
    ENST00000531717 ENST00000531644 ENST00000532108(uc009yek.2) ENST00000533021


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    hsa-miR-24 hsa-miR-3647-3p hsa-miR-4267
    SwitchGear 3'UTR luciferase reporter plasmidTRIM68 3' UTR sequence
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    Additional cDNA sequence: 

    AF360739.1 AF439153.1 AK001231.1 AK022923.1 AK056523.1 AK291166.1 AK301120.1 AK310888.1 
    AL832730.1 AY005802.1 BC075058.2 BC109063.2 

    5 DOTS entries:

    DT.75157640  DT.120723693  DT.100773686  DT.441571  DT.91756173 

    24/64 AceView cDNA sequences (see all 64):

    BF195728 AW294298 N39251 BU726330 AA595261 BC075058 AW438826 AI858536 
    AA576931 AF439153 CR605215 NM_018073 W68593 BM548233 BX454582 CD628937 
    BU194619 AF360739 CF144686 BE867271 AW274746 AU128493 AK001231 AU124051 

    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for TRIM68    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7
    SP1:                                -               
    SP2:        -                                       


    ECgene alternative splicing isoforms for TRIM68

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TRIM68 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TATGTAGGAG

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See TRIM68 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TRIM68

    SOURCE GeneReport for Unigene cluster: Hs.523438

    UniProtKB/Swiss-Prot: TRI68_HUMAN, Q6AZZ1
    Tissue specificity: Widely expressed. Expressed at high levels in prostate cancer cell lines. Up-regulation could be
    restricted to androgen-dependent cells

        SABiosciences Expression via Pathway-Focused PCR Array including TRIM68: 
              Ubiquitin Ligases in human mouse rat

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for TRIM68 gene from 2/7 species (see all 7)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia TRIM586
    --
    40(a)
    1 → many
    GL343252.1(1250786-1269672)
    zebrafish
    (Danio rerio)
    Actinopterygii trim476
    tripartite motif containing 47
    22(a)
    possible ortholog
    10(26752552-26762609)


    ENSEMBL Gene Tree for TRIM68 (if available)
    TreeFam Gene Tree for TRIM68 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TRIM68 gene
    TRIM42  TRIM72  TRIM262  TRIM392  TRIM342  TRIML12  TRIM272  TRIM582  
    TRIM102  TRIM152  TRIM602  TRIM222  TRIM172  TRIM52  TRIM62  TRIM6-TRIM342  
    TRIM212  TRIM382  TRIM39-RPP212  TRIM112  
    18/44 SIMAP similar genes for TRIM68 using alignment to 4 protein entries:     TRI68_HUMAN (see all proteins) (see all similar genes):
    ERMAP    TRIM21    TRIM5    BT3.3    TRIM22    TRIM58
    TRIM6    TRIM27    TRIM41    DKFZp686F23130    TRIM60    TRIM26
    TRIM39    TRIM17    TRIM11    TRIM38    TRIM31    BTNL9

    TRIM68 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/278 NCBI SNPs in TRIM68 are shown (see all 278    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1490594471,2
    Cother4621686(+) TCCACG/AAAGAT 2 /F syn11Minor allele frequency- A:0.00NA 4552
    rs122826611,2
    C,F,A,H,--4619424(+) CTGGTC/TGTAAA 1 -- int15Minor allele frequency- T:0.03NS EA NA 540
    rs1892891491,2
    --4619437(+) TCAGAC/TAATCT 1 -- int10--------
    rs1819052561,2
    --4619502(+) CCAAAC/GAGGCT 1 -- int10--------
    rs18760261,2
    C,A,H,--4619532(-) TGGTTA/GGTTTG 1 -- int113Minor allele frequency- G:0.07NA WA CSA EA 378
    rs781651631,2
    C,F,--4619543(+) AAAAGA/CGTACA 1 -- int12Minor allele frequency- C:0.06WA NA 238
    rs1849897371,2
    --4619544(+) AAAGAA/GTACAT 1 -- int10--------
    rs672618731,2
    C,F,--4619666(+) CAACAT/CTGATT 1 -- int15Minor allele frequency- C:0.11NA WA 244
    rs342825981,2
    C,F,--4619690(+) TTTGTC/GCCAAT 1 -- int17Minor allele frequency- G:0.11NA WA CSA EA 366
    rs1903311641,2
    --4619694(+) TCCCAA/GTTGTC 1 -- int10--------

    HapMap Linkage Disequilibrium report for TRIM68 (4619902 - 4629489 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for TRIM68: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TRIM68 for disorders           About GeneDecksing

    OMIM gene information: 613184    OMIM disorders: --

    6 diseases for TRIM68:    About MalaCards
    prostate cancer    prostatitis    systemic lupus erythematosus    lupus erythematosus
    sjogren's syndrome    nephropathy

    Human Genome Epidemiology (HuGE) Navigator: TRIM68 (1 document)

    Export disorders for TRIM68 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TRIM68 gene, integrated from 9 sources (see all 19):
    (articles sorted by number of sources associating them with TRIM68)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A novel gene on human chromosome 2p24 is differentially expressed between androgen-dependent and androgen-independent prostate cancer cells. (PubMed id 11597395)1, 2, 3 Chang G.T.... Brinkmann A.O. (2001)
    2. TRIM68 regulates ligand-dependent transcription of androgen receptor in prostate cancer cells. (PubMed id 18451177)1, 2 Miyajima N....Hatakeyama S. (2008)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. SS-56, a novel cellular target of autoantibody responses in Sjogren syndrome and systemic lupus erythematosus. (PubMed id 11560955)1, 2 Billaut-Mulot O.... Bahr G.M. (2001)
    6. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    7. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
    8. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    9. Genome-wide linkage scans for type 2 diabetes mellitu s in four ethnically diverse populations-significant evidence for linkage on ch romosome 4q in African Americans: the Family Investigation of Nephropathy and D iabetes Research Group. (PubMed id 19795399)1 Malhotra A....Hanson R.L. (2009)
    10. A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. (PubMed id 19690564)1 van Wijk S.J....Timmers H.T. (2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 55128 HGNC: 21161 AceView: RNF137 Ensembl:ENSG00000167333 euGenes: HUgn55128
    ECgene: TRIM68 H-InvDB: TRIM68

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TRIM68 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for TRIM68 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for TRIM68 gene:
    Search GeneIP for patents involving TRIM68

    GeneCards and IP:
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