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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

TRIM63 Gene

protein-coding GIFtS: 61
GCID: GC01M026377

tripartite motif containing 63, E3 ubiquitin protein ligase

(Previous names: ring finger protein 28, tripartite motif-containing 63,...)
(Previous symbol: RNF28)

Explore 22 diseases affiliated with
TRIM63 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Tripartite Motif Containing 63, E3 Ubiquitin Protein Ligase¹ ²		MURF1² ³
RNF28¹ ² ³ ⁵		MURF-1¹
SMRZ¹ ² ³ ⁵		Tripartite Motif Containing 63¹
RING Finger Protein 28¹ ² ³		MURF2²
IRF¹ ² ³		E3 Ubiquitin-Protein Ligase TRIM63²
Tripartite Motif-Containing 63¹ ²		Muscle Specific Ring Finger Protein 2²
Iris RING Finger Protein² ³		EC 6.3.2.-³
Muscle-Specific RING Finger Protein 1² ³		MuRF-1³
Striated Muscle RING Zinc Finger Protein² ³		MuRF1³
Tripartite Motif-Containing Protein 63² ³

External Ids:	HGNC: 16007¹	Entrez Gene: 84676²	Ensembl: ENSG00000158022⁷	OMIM: 606131⁵	UniProtKB: Q969Q1³

Export aliases for TRIM63 gene to outside databases

Previous GC identifers: GC01M026062 GC01M026250 GC01M024631

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TRIM63:

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of

this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays

an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy

chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. (provided by RefSeq,

Feb 2012)

UniProtKB/Swiss-Prot: TRI63_HUMAN, Q969Q1

Function: E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and

HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is

catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino

acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other

sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an

anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle

cells

Gene Wiki entry for TRIM63

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000001.10 NC_018912.1 NT_004610.19

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the TRIM63 gene promoter:
Max1 USF1 STAT1 Sp1 CP2 USF-1 IRF-7A ZIC2/Zic2 c-Myc
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: TRIM63 promoter sequence

Search SABiosciences Chromatin IP Primers for TRIM63

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM63

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p34-p33 Ensembl cytogenetic band: 1p36.11 HGNC cytogenetic band: 1p34-p33

TRIM63 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TRIM63 gene location

GeneLoc information about chromosome 1 GeneLoc Exon Structure

GeneLoc location for GC01M026377: view genomic region (about GC identifiers)

Start:	26,377,795 bp from pter	End:	26,394,927 bp from pter
Size:	17,133 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TRI63_HUMAN, Q969Q1 (See protein sequence)

Recommended Name: E3 ubiquitin-protein ligase TRIM63

Size: 353 amino acids; 40248 Da

Subunit: Homodimer. Homooligomer and heterooligomer. Interacts with SUMO2, titin/TTN and GMEB1. Interacts with TRIM54

and probably with TRIM55 and TNNI3. Forms a ternary complex with GNB2L1 and PRKCE (By similarity). Interacts with CKM

Subcellular location: Cytoplasm. Nucleus (By similarity). Cytoplasm, myofibril, sarcomere, M line. Cytoplasm,

myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines

in skeletal muscle

Developmental stage: Expressed throughout all developmental stages

Caution: It is uncertain whether Met-1 or Met-14 is the initiator

Sequence caution: Sequence=AAK52497.1; Type=Frameshift; Positions=Several; Sequence=AAK52497.1; Type=Miscellaneous

discrepancy; Note=Sequencing errors; Sequence=CAC33173.1; Type=Erroneous initiation;

2 PDB 3D structures from and Proteopedia for TRIM63:

2D8U (3D)

3DDT (3D)

Secondary accessions: Q5T2I1 Q96BD3 Q96KD9 Q9BYV4

Explore the universe of human proteins at neXtProt for TRIM63: NX_Q969Q1

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q969Q1

TRIM63 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_115977.2

ENSEMBL proteins:

ENSP00000363390

Reactome Protein details: Q969Q1
Human Recombinant Protein Products for TRIM63:

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	Novus Biologicals TRIM63 Proteins Novus Biologicals TRIM63 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for TRIM63

Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--
GO:0005737	cytoplasm	NAS	11243782
GO:0005874	microtubule	NAS	11243782
GO:0043292	contractile fiber	IEA	--

TRIM63 for ontologies About GeneDecksing

TRIM63 Antibody Products:

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	Novus Biologicals TRIM63 Antibodies
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	Uscn Antibodies for TRIM63
	ThermoFisher Antibody for TRIM63

Assay Products for TRIM63:

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	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	R&D Systems Ubiquitin Proteasome Pathway (UPP) Assay Kits & Reagents for TRIM63 (MuRF1/TRIM63)
	GenScript Custom Assay Services for TRIM63
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for TRIM63

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TRIM63 for domains About GeneDecksing

5 InterPro domains/families:

IPR017907 Znf_RING_CS

IPR001841 Znf_RING

IPR017903 COS_domain

IPR000315 Znf_B-box

IPR013083 Znf_RING/FYVE/PHD

Graphical View of Domain Structure for InterPro Entry Q969Q1

ProtoNet protein and cluster: Q969Q1

2 Blocks protein families:

IPB000315 B-box zinc finger signature

IPB001841 Zn-finger

UniProtKB/Swiss-Prot: TRI63_HUMAN, Q969Q1

Domain: The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for

the E3 ubiquitin ligase activity (By similarity)

Domain: The B box-type zinc finger mediates homodimerization

Similarity: Contains 1 B box-type zinc finger

Similarity: Contains 1 COS domain

Similarity: Contains 1 RING-type zinc finger

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: TRI63_HUMAN, Q969Q1

Function: E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and

HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is

catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino

acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other

sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an

anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle

cells

Enzyme Number (IUBMB): EC 6.3.2.-¹

Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004842	ubiquitin-protein ligase activity	IEA	--
GO:0004871	signal transducer activity	NAS	11243782
GO:0005515	protein binding	--	--
GO:0008270	zinc ion binding	IEA	--
GO:0031432	titin binding	IDA	11927605

TRIM63 for ontologies About GeneDecksing

Phenotypes:
2 GenomeRNAi human phenotypes for TRIM63:

Decreased viability with pacli

Increased G2M DNA content, inc

8 MGI mutant phenotypes (inferred from 4 alleles) (MGI details for Trim63):

adipose tissue	cardiovascular system	growth/size	homeostasis/metabolism	liver/biliary system
mortality/aging	muscle	respiratory system

TRIM63 for phenotypes About GeneDecksing

Animal Models:
Mouse knock-outs for TRIM63: Trim63^tm1Slbt Trim63^tm2.1Vlcg

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for TRIM63

miRNA Products:		miRTarBase miRNAs that target TRIM63: hsa-mir-29a (MIRT005909)

		Browse 3'-UTR reporter clones for miRNA target validation Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TRIM63
		8/20 QIAGEN miScript miRNA Assays for microRNAs that regulate TRIM63 (see all 20):
		hsa-miR-498 hsa-miR-300 hsa-miR-29a hsa-miR-29c hsa-miR-767-5p hsa-miR-507 hsa-miR-3921 hsa-miR-23a

		SwitchGear 3'UTR luciferase reporter plasmid: TRIM63 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TRIM63 (see all 7) OriGene shRNA RFP: TRIM63 OriGene siRNA: TRIM63
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TRIM63
		Sirion Biotech Custom design and validation of potent shRNA sequences against TRIM63

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for TRIM63
			Sirion Biotech Customized adenovirus for overexpression of TRIM63 Sirion Biotech Customized adenovirus for potent knockdown of TRIM63

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM63 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM63 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TRIM63 (NM_032588)
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		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TRIM63
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM63

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for TRIM63
		Search LifeMap BioReagents cell lines for TRIM63
		Sirion Biotech Customized stable knockdown cell line services for TRIM63 Sirion Biotech Customized inducible knockdown cell line services for TRIM63 Sirion Biotech Customized stable overexpressing cell line services for TRIM63 Sirion Biotech Customized inducible overexpressing cell line services for TRIM63

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM63

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Antigen processing: Ubiquitination & Proteasome degradation

	Antigen processing: Ubiquitination & Proteasome degradation	1.00			Polyubiquitination of substrate	0.75
	Class I MHC mediated antigen processing & presentation	0.83

Immune System

Immune System

1.00

Adaptive Immune System

0.59

TWEAK Pathway

TWEAK Signaling Pathway

0.31

Pathway sources
See GeneCards unified pathways
Show all pathways

1 BioSystems Pathway for TRIM63

TWEAK Signaling Pathway

5 Reactome Pathways for TRIM63

	Antigen processing: Ubiquitination & Proteasome degradation
	Polyubiquitination of substrate
	Adaptive Immune System
	Class I MHC mediated antigen processing & presentation
	Immune System

UniProtKB/Swiss-Prot: TRI63_HUMAN, Q969Q1

Pathway: Protein modification; protein ubiquitination

TRIM63 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for TRIM63

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/64 Interacting proteins for TRIM63 (Q969Q1^{2, 3} ENSP00000363390⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 64)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
GFM1	Q96RP9²^,³, ENSP00000419038⁴	MINT-6492209 MINT-6503520 I2D: score=1 STRING: ENSP00000419038
GMEB1	Q9Y692²^,³, ENSP00000294409⁴	MINT-6503470 I2D: score=3 STRING: ENSP00000294409
SQSTM1	Q13501²^,³, ENSP00000374455⁴	MINT-6503660 I2D: score=2 STRING: ENSP00000374455
TNNT1	P13805²^,³, ENSP00000291901⁴	MINT-6503640 I2D: score=2 STRING: ENSP00000291901
TTN	Q8WZ42²^,³	MINT-6503430 MINT-6503580 I2D: score=2

About this table

Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006936	muscle contraction	IEA	--
GO:0007165	signal transduction	NAS	11243782
GO:0014732	skeletal muscle atrophy	IEA	--
GO:0016567	protein ubiquitination	--	--
GO:0043161	proteasomal ubiquitin-dependent protein catabolic process	IEA	--

TRIM63 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

TRIM63 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for TRIM63

4 HMDB Compounds for TRIM63 About this table

Compound	Synonyms	CAS #	PubMed Ids
Adenosine monophosphate	5'-AMP (see all 28)	61-19-8	--
Adenosine triphosphate	5'-(tetrahydrogen triphosphate) Adenosine (see all 24)	56-65-5	--
Phosphoric acid	acide phosphorique (FRENCH) (see all 20)	7664-38-2	--
Pyrophosphate	(4-)Diphosphoric acid ion (see all 10)	14000-31-8	--

10 Novoseek chemical compound relationships for TRIM63 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
diamide	38.8	3	7508861 (1), 1738601 (1)
rantes	35.3	2	10893229 (1), 20197549 (1)
2-mercaptoethanol	33.2	4	2157191 (1), 7508861 (1), 1738601 (1)
iron	27.4	39	8464737 (4), 8130687 (4), 7508861 (3), 7741221 (3) (see all 11)
serine	24.9	2	11846979 (1), 17200120 (1)
5-aminolevulinic acid	23	2	8021254 (1), 7881153 (1)
tyrosine	5.01	5	17200120 (3), 9765473 (2)
retinoic acid	3.63	2	9669677 (2)
lysine	0	1	12374802 (1)
nitric oxide	0	2	15296650 (1), 7881153 (1)

Search CenterWatch for drugs/clinical trials and news about TRIM63 / TRI63

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for TRIM63 gene:

NM_032588.3

Unigene Cluster for TRIM63:

Tripartite motif containing 63, E3 ubiquitin protein ligase

Hs.279709 [show with all ESTs]

Unigene Representative Sequence: AF353673

2 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000374272(uc001bli.1) ENST00000483052

miRNA Products:		Browse 3'-UTR reporter clones for miRNA target validation Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TRIM63
		8/20 QIAGEN miScript miRNA Assays for microRNAs that regulate TRIM63 (see all 20):
		hsa-miR-498 hsa-miR-300 hsa-miR-29a hsa-miR-29c hsa-miR-767-5p hsa-miR-507 hsa-miR-3921 hsa-miR-23a

		SwitchGear 3'UTR luciferase reporter plasmid: TRIM63 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TRIM63 (see all 7) OriGene shRNA RFP: TRIM63 OriGene siRNA: TRIM63
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		Sirion Biotech Custom design and validation of potent shRNA sequences against TRIM63

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM63 (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM63 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TRIM63 (NM_032588)
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		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM63

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM63 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat TRIM63
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TRIM63
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TRIM63

Additional cDNA sequence:

AF353673.1 AJ276484.1 AK056942.1 AK297820.1 BC080529.1 BT007373.1

2 DOTS entries:

DT.436990 DT.95264585

24/30 AceView cDNA sequences (see all 30):

BI754358 BF724411 BG765856 AK056942 AJ276484 NM_032588 F35868 BX283938

F36292 C02645 BX500807 BG769996 AF353673 AI351421 BC080529 BG764060

BG761218 BI758535 BX500821 AW296154 F21990 F25523 BT007373 BE894237

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

TRIM63 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GACTTCTTTA

About this image

TRIM63 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

1 LifeMap In Vivo Development Anatomical Compartment/Cell

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Bone

Zeugopod Periosteum

Bone

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

Beating cell clusters (Spontaneous differen...)

See TRIM63 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TRIM63

SOURCE GeneReport for Unigene cluster: Hs.279709

UniProtKB/Swiss-Prot: TRI63_HUMAN, Q969Q1

Tissue specificity: Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris

SABiosciences Expression via Pathway-Focused PCR Arrays including TRIM63:

Skeletal Muscle: Myogenesis & Myopathy in human mouse rat

Ubiquitin Ligases in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM63 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat TRIM63
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TRIM63
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TRIM63

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM63

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for TRIM63 gene from 3/13 species (see all 13) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	TRIM63¹	tripartite motif containing 63	76.8(n) 75.15(a)		426754 XM_424369.3 XP_424369.3
lizard (Anolis carolinensis)	Reptilia	TRIM63⁶	--	69(a)	1 ↔ 1	GL343480.1(296364-313859)
zebrafish (Danio rerio)	Actinopterygii	zgc56376²	similar to ring finger protein 30	78.05(n)		394070 BC049414.1

ENSEMBL Gene Tree for TRIM63 (if available)
TreeFam Gene Tree for TRIM63 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for TRIM63 gene

TRIM13² FSD1L² TRIM46² TRIM59² TRIM67² TRIM9² TRIM36² MID1²

TRIM55² MID2² TRIM54² FSD1²

2 SIMAP similar genes for TRIM63 using alignment to 1 protein entry: TRI63_HUMAN:

TRIM55 TRIM54

TRIM63 for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
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SNP ID	Valid	Clinical significance	Chr 1 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs7555796^1,2	A	--	26377446(+)	`catgaA/Gccact`	1	--	ds500¹		0	--	--	--	--
rs189843745^1,2		--	26377456(+)	`TGCACC/TTGCCC`	1	--	ds500¹		0	--	--	--	--
rs7551311^1,2	A	--	26377523(+)	`GAGCAC/TGGGGA`	1	--	ds500¹		0	--	--	--	--
rs6689407^1,2	C,F,A,H	--	26377595(+)	`CCAGAA/GCAACA`	1	--	ds500¹		11		NS EA NA WA	658
rs6703821^1,2	C,F,H	--	26377603(+)	`ACAACA/GCATTG`	1	--	ds500¹		6		NS EA WA	538
rs7555970^1,2	A	--	26377605(+)	`aacaCA/GTTGAA`	1	--	ds500¹		0	--	--	--	--
rs151141393^1,2		--	26377608(+)	`ACATTA/GAACCT`	1	--	ds500¹		0	--	--	--	--
rs139144998^1,2		--	26377766(+)	`CCAGGA/GAAAAA`	1	--	ds500¹		0	--	--	--	--
rs181889987^1,2		--	26377774(+)	`AAAAGA/GCTGAA`	1	--	ds500¹		0	--	--	--	--
rs7542815^1,2	A,H	--	26377789(+)	`ACCCCG/AGCTCC`	1	--	ds500¹		4		NS EA	418

HapMap Linkage Disequilibrium report for TRIM63 (26377795 - 26394927 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for TRIM63: --
Human Gene Mutation Database (HGMD): TRIM63

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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TRIM63 for disorders           About GeneDecksing

OMIM gene information: 606131    OMIM disorders: --

20/22 diseases for TRIM63 (see all 22):    About MalaCards

alveolar soft part sarcoma chronic obstructive pulmonary disease kaposi's sarcoma muscular atrophy

myocardial infarction behcet's disease pulmonary disease sjogren's syndrome

sarcoma multiple myeloma uremia rheumatoid arthritis

hepatitis c paralysis hyperthyroidism myeloma

arthritis hepatitis neuroblastoma retinitis

3 diseases from the University of Copenhagen DISEASES database for TRIM63:

Muscular atrophy Myopathy of critical illness Alveolar soft part sarcoma

5 Novoseek disease relationships for TRIM63 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
virus infection	51.7	6	9463386 (1), 16751392 (1), 7536924 (1), 18440553 (1) (see all 6)
atrophy	43.7	16	19168726 (3), 16250905 (1), 15100091 (1), 17008454 (1) (see all 9)
kaposi sarcoma	30.1	2	11160738 (1), 11846976 (1)
hypertrophy	0	3	19859778 (1), 15125842 (1)
tumors	0	2	18511292 (1), 9669677 (1)

Export disorders for TRIM63 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for TRIM63 gene, integrated from 9 sources (see all 155):
(articles sorted by number of sources associating them with TRIM63)

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Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. (PubMed id 11243782)^{1, 2, 3} Centner T....Labeit S. (2001)
A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain. (PubMed id 11283016)^{1, 2, 3} Dai K.S. and Liew C.C. (2001)
MuRF1-dependent regulation of systemic carbohydrate metabolism as revealed from transgenic mouse studies. (PubMed id 18468620)^{1, 2, 9} Hirner S....Labeit D. (2008)
Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1. (PubMed id 11927605)^{1, 2, 9} McElhinny A.S.... Gregorio C.C. (2002)
Structural analysis of B-Box 2 from MuRF1: identification of a novel self-association pattern in a RING-like fold. (PubMed id 18795805)^{1, 2} Mrosek M....Mayans O. (2008)
Muscle RING-finger protein-1 (MuRF1) as a connector o f muscle energy metabolism and protein synthesis. (PubMed id 18222470)^{1, 2} Koyama S....Sorimachi H. (2008)
The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)^{1, 2} Gregory S.G.... Bentley D.R. (2006)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Expressed sequence tag analysis of adult human iris for the NEIBank project: steroid-response factors and similarities with retinal pigment epithelium. (PubMed id 12107412)^{1, 2} Wistow G.... Peterson K. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 84676	HGNC: 16007	AceView: RNF28	Ensembl:ENSG00000158022	euGenes: HUgn84676
ECgene: TRIM63	H-InvDB: TRIM63

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for TRIM63	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for TRIM63	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for TRIM63 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for TRIM63 gene:
Search GeneIP for patents involving TRIM63

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

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TRIM63 Gene

tripartite motif containing 63, E3 ubiquitin protein ligase

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TRIM63 (Gene Symbol)
Tripartite Motif Containing 63, E3 Ubiquitin Protein Ligase
RNF28
SMRZ
RING Finger Protein 28
IRF
Tripartite Motif-Containing 63
Iris RING Finger Protein
Muscle-Specific RING Finger Protein 1
Striated Muscle RING Zinc Finger Protein
Tripartite Motif-Containing Protein 63
MURF1
MURF-1
Tripartite Motif Containing 63
MURF2
E3 Ubiquitin-Protein Ligase TRIM63
Muscle Specific Ring Finger Protein 2
EC 6.3.2.-
MuRF-1
MuRF1

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TRIM63 Gene

tripartite motif containing 63, E3 ubiquitin protein ligase

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