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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

TRIM28 Gene

protein-coding GIFtS: 61
GCID: GC19P059055

tripartite motif containing 28

(Previous name: tripartite motif-containing 28 )

Explore 11 diseases affiliated with
TRIM28 via

MalaCards

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Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Tripartite Motif Containing 28¹ ²		KRIP-1² ³
KAP1¹ ² ³		TIF1-Beta¹
RNF96¹ ² ³		E3 SUMO-Protein Ligase TRIM28² ³
TIF1B¹ ² ³		KRAB-Associated Protein 1² ³
TF1B¹ ²		KRAB-Interacting Protein 1² ³
Tripartite Motif-Containing 28¹ ²		RING Finger Protein 96² ³
Nuclear Corepressor KAP-1² ³		Transcription Intermediary Factor 1-Beta²
Tripartite Motif-Containing Protein 28² ³		Transcriptional Intermediary Factor 1-Beta²
KAP-1² ³		EC 6.3.2.-³

External Ids:	HGNC: 16384¹	Entrez Gene: 10155²	Ensembl: ENSG00000130726⁷	OMIM: 601742⁵	UniProtKB: Q13263³

Export aliases for TRIM28 gene to outside databases

Previous GC identifers: GC19P059954 GC19P059509 GC19P063731 GC19P063747 GC19P063553 GC19P055366

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TRIM28:

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box

repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to

associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite

motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

(provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: TIF1B_HUMAN, Q13263

Function: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by

recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which

specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances

transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14'

acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression.

Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the

transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1

complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in

the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its

PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.

Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and

possibly MAGEA3 and MAGEA6

Gene Wiki entry for TRIM28

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000019.9 NC_018930.1 NT_011109.16

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the TRIM28 gene promoter:
SRF SRF (504 AA) AP-4 CUTL1 HNF-4alpha1 MZF-1 PPAR-gamma1 Pax-3 PPAR-gamma2 MRF-2
Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids

Search SABiosciences Chromatin IP Primers for TRIM28

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM28

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.4 Ensembl cytogenetic band: 19q13.43 HGNC cytogenetic band: 19q13.4

TRIM28 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TRIM28 gene location

GeneLoc information about chromosome 19 GeneLoc Exon Structure

GeneLoc location for GC19P059055: view genomic region (about GC identifiers)

Start:	59,055,836 bp from pter	End:	59,062,082 bp from pter
Size:	6,247 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TIF1B_HUMAN, Q13263 (See protein sequence)

Recommended Name: Transcription intermediary factor 1-beta

Size: 835 amino acids; 88550 Da

Subunit: Oligomer; the RBCC domain homotrimerizes and interacts with one molecule of KRAB to form the KRAB-KAP1

corepressor complex. Binding to a KRAB domain is an absolute requirement for silencing gene expression. Interacts with

CEBPB and NR3C1 (By similarity). Interacts with a number of KRAB-ZFP proteins including ZNF10, ZFP53, ZFP68, ZNF382

and ZNF256. Interacts with NCOR1, NR3C1 and CHD3. Interacts with CEBPB (via the RING-type and PHD-type zinc fingers).

Component of a ternary complex that includes TRIM28, a HP1 protein (CBX1, CBX3 OR CBX5), a KRAB domain-containing

protein, and DNA. Interacts with CBX5 (via the PxVxL motif); the interaction occurs in interphase nuclei and competes

for binding POGZ. Interacts with POGZ; the interaction competes for interaction with CBX5. Interacts with SETDB1; the

interaction is enhanced by KAP1 sumoylation, stimulates SETB1 histone methyltransferase activity and gene silencing.

Interacts (via the PHD-type zinc finger) with UBE2I; the interaction is required for sumoylation and repressor

activity. Component of the TRIM28/KAP1-ERBB4-MDM2 complex involved in connecting growth factor and DNA damage

responses. Interacts directly with ERBB4; the interaction represses ERBB4-mediated transcription activity. Interacts

with MDM2; the interaction contributes to p53/TP53 inactivation. Component of the TRIM28/KAP1-MDM2-p53/TP53; involved

in regulating p53/TP53 stabilization and activity. Interacts (via the leucine zipper alpha helical coiled-coil) with

E2F1 (central region); the interaction inhibits E2F1 acetylation and transcriptional activity. Interacts with PPP1CA;

the interaction dephosphorylates TRIM28 at Ser-824 and forms a complex at the p21 promoter site. Interacts with

PPP1CB; the interaction is weak but is increased on dephosphorylation at Ser-824. Interacts with FES/FPS. Interacts

with SMARCAD1. Interacts with, and sumoylates IRF7. Interacts with MAGEC2. Part of a complex composed of TRIM28,

HDAC1, HDAC2 and EHMT2

Subcellular location: Nucleus. Note=Associated with centromeric heterochromatin during cell differentiation through

CBX1 (By similarity)

3 PDB 3D structures from and Proteopedia for TRIM28:

1FP0 (3D)

2RO1 (3D)

2YVR (3D)

Secondary accessions: O00677 Q7Z632 Q93040 Q96IM1

Alternative splicing: 2 isoforms: Q13263-1 Q13263-2 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for TRIM28: NX_Q13263

Post-translational modifications:

ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of

genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the

phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes¹

Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for

interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation.

Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A

and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA

damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21¹

Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q13263

TRIM28 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_005753.1

ENSEMBL proteins:

ENSP00000253024 ENSP00000342232

Reactome Protein details: Q13263
Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate: TRIM28 OriGene Custom Protein Services for TRIM28
	GenScript Custom Purified and Recombinant Proteins Services for TRIM28
	Novus Biologicals TRIM28 Proteins Novus Biologicals TRIM28 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Browse Proteins at Uscn

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000785	chromatin	--	--
GO:0005634	nucleus	ISS	--
GO:0005654	nucleoplasm	TAS	--
GO:0005719	nuclear euchromatin	IEA	--
GO:0005720	nuclear heterochromatin	IEA	--

TRIM28 for ontologies About GeneDecksing

TRIM28 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of TRIM28
	R&D Systems Antibodies for TRIM28 (KAP1)
	Cell Signaling Technology (CST) Antibodies for TRIM28 (TIF1B)
	OriGene Antibodies (see all 3): TRIM28 OriGene Custom Antibody Services for TRIM28
	GenScript Custom Superior Antibodies Services for TRIM28
	Novus Biologicals TRIM28 Antibodies
	Abcam antibodies for TRIM28
	Browse Antibodies at Uscn
	ThermoFisher Antibody for TRIM28

Assay Products for TRIM28:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for TRIM28
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs and CLIAs at Uscn

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TRIM28 for domains About GeneDecksing

5/9 InterPro domains/families (see all 9):

IPR003649 Bbox_C

IPR019786 Zinc_finger_PHD-type_CS

IPR001841 Znf_RING

IPR001965 Znf_PHD

IPR001487 Bromodomain

Graphical View of Domain Structure for InterPro Entry Q13263

ProtoNet protein and cluster: Q13263

4 Blocks protein families:

IPB000315 B-box zinc finger signature

IPB001841 Zn-finger

IPB001965 Zn-finger-like

IPB003649 B-box

UniProtKB/Swiss-Prot: TIF1B_HUMAN, Q13263

Domain: The HP1 box is both necessary and sufficient for HP1 binding

Domain: The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the

bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins.

Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain

Domain: The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB

domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the

leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization

Domain: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains.

This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain

Similarity: Belongs to the TRIM/RBCC family

Similarity: Contains 2 B box-type zinc fingers

Similarity: Contains 1 bromo domain

Similarity: Contains 1 PHD-type zinc finger

Similarity: Contains 1 RING-type zinc finger

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary: