Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

TRIM25 Gene

protein-coding GIFtS: 61
GCID: GC17M054965

tripartite motif containing 25

(Previous names: zinc finger protein 147 (estrogen-responsive finger protein),...)
(Previous symbol: ZNF147)

Explore 8 diseases affiliated with
TRIM25 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Tripartite Motif Containing 25¹ ²		RING Finger Protein 147² ³
EFP¹ ² ³ ⁵		Z147²
ZNF147¹ ² ³ ⁵		E3 Ubiquitin/ISG15 Ligase TRIM25²
RNF147¹ ² ³		Tripartite Motif Protein TRIM25²
Tripartite Motif-Containing 25¹ ²		Zinc Finger Protein-147²
Zinc Finger Protein 147 (Estrogen-Responsive Finger Protein)¹ ²		EC 6.3.2.19³
Estrogen-Responsive Finger Protein² ³		EC 6.3.2.N3¹
Tripartite Motif-Containing Protein 25² ³		Zinc Finger Protein 147³
Ubiquitin/ISG15-Conjugating Enzyme TRIM25² ³

External Ids:	HGNC: 12932¹	Entrez Gene: 7706²	Ensembl: ENSG00000121060⁷	OMIM: 600453⁵	UniProtKB: Q14258³

Export aliases for TRIM25 gene to outside databases

Previous GC identifers: GC17M055444 GC17M052323 GC17M050325

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TRIM25:

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three

zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to

the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this

protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene

is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary

response gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: TRI25_HUMAN, Q14258

Function: Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against

viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal

CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of

interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated

in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs

Gene Wiki entry for TRIM25

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000017.10 NC_018928.1 NT_010783.15

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the TRIM25 gene promoter:
AhR STAT1 Pax-5 p53 Arnt FOXJ2 (long isoform) FOXJ2 ATF6
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): TRIM25 promoter sequence

Search SABiosciences Chromatin IP Primers for TRIM25

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM25

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q23.2 Ensembl cytogenetic band: 17q22 HGNC cytogenetic band: 17q23.1

TRIM25 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TRIM25 gene location

GeneLoc information about chromosome 17 GeneLoc Exon Structure

GeneLoc location for GC17M054965: view genomic region (about GC identifiers)

Start:	54,965,270 bp from pter	End:	54,991,409 bp from pter
Size:	26,140 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TRI25_HUMAN, Q14258 (See protein sequence)

Recommended Name: E3 ubiquitin/ISG15 ligase TRIM25

Size: 630 amino acids; 70973 Da

Subunit: Interacts (via SPRY domain) with DDX58 (via CARD domain). Interacts (via coiled coil) with influenza A virus

NS1 protein; this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD

ubiquitination, thereby suppressing DDX58 signal transduction

Subcellular location: Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies

Explore the universe of human proteins at neXtProt for TRIM25: NX_Q14258

Post-translational modifications:

Auto-ISGylated¹

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q14258

TRIM25 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_005073.2

ENSEMBL proteins:

ENSP00000323889 ENSP00000459980 ENSP00000445961

Reactome Protein details: Q14258
Human Recombinant Protein Products for TRIM25:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein: TRIM25 OriGene Protein Over-expression Lysate: TRIM25 OriGene Custom Protein Services for TRIM25
	GenScript Custom Purified and Recombinant Proteins Services for TRIM25
	Novus Biologicals TRIM25 Proteins Novus Biologicals TRIM25 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for TRIM25

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	--
GO:0005829	cytosol	TAS	--
GO:0030054	cell junction	IDA	--

TRIM25 for ontologies About GeneDecksing

TRIM25 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	Cell Signaling Technology (CST) Antibodies for TRIM25
	OriGene Antibodies (see all 2): TRIM25 OriGene Custom Antibody Services for TRIM25
	GenScript Custom Superior Antibodies Services for TRIM25
	Novus Biologicals TRIM25 Antibodies
	Abcam antibodies for TRIM25
	Uscn Antibodies for TRIM25
	ThermoFisher Antibodies for TRIM25

Assay Products for TRIM25:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for TRIM25
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for TRIM25

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TRIM25 for domains About GeneDecksing

5/9 InterPro domains/families (see all 9):

IPR006574 PRY

IPR017907 Znf_RING_CS

IPR001841 Znf_RING

IPR001870 B30.2/SPRY

IPR003877 SPRY_rcpt

Graphical View of Domain Structure for InterPro Entry Q14258

ProtoNet protein and cluster: Q14258

4 Blocks protein families:

IPB000315 B-box zinc finger signature

IPB001841 Zn-finger

IPB003879 Butyrophylin C-terminal DUF signature

IPB006574 SPRY-associated domain

UniProtKB/Swiss-Prot: TRI25_HUMAN, Q14258

Domain: The RING-type zinc finger is important for ISG15 E3 ligase activity and autoISGylation. AutoISGylation

negatively regulates ISG15 E3 ligase activity

Domain: The C-terminal B30.2/SPRY domain interacts with the first N-terminal CARD domain of DDX58

Similarity: Contains 1 B30.2/SPRY domain

Similarity: Contains 1 RING-type zinc finger

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: TRI25_HUMAN, Q14258

Function: Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against

viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal

CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of

interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated

in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine

Catalytic activity: ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine

Induction: By interferons

Enzyme Numbers (IUBMB): EC 6.3.2.19¹ EC 6.3.2.n3¹

Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003700	sequence-specific DNA binding transcription factor activity	TAS	8248217
GO:0004842	ubiquitin-protein ligase activity	IEA	--
GO:0005515	protein binding	IPI	19454348
GO:0008270	zinc ion binding	IEA	--

TRIM25 for ontologies About GeneDecksing

Phenotypes:
6 GenomeRNAi human phenotypes for TRIM25:

Decreased Salmonella enterica	Decreased Salmonella enterica	Decreased Salmonella enterica	Decreased Salmonella enterica
Decreased Salmonella-containin	Increased G1 DNA content

1 MGI mutant phenotype (inferred from 1 allele) (MGI details for Trim25):

reproductive system

TRIM25 for phenotypes About GeneDecksing

Animal Models:
Mouse knock-out Trim25^tm1Mura for TRIM25

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for TRIM25

miRNA Products:		OriGene 3'-UTR Clone: TRIM25 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TRIM25
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate TRIM25 (see all 14):
		hsa-miR-3671 hsa-miR-345 hsa-miR-1283 hsa-miR-520d-5p hsa-miR-340 hsa-miR-544 hsa-miR-298 hsa-miR-2276

		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TRIM25 (see all 7) OriGene shRNA RFP: TRIM25 OriGene siRNA: TRIM25
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TRIM25
		Sirion Biotech Custom design and validation of potent shRNA sequences against TRIM25

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for TRIM25
			Sirion Biotech Customized adenovirus for overexpression of TRIM25 Sirion Biotech Customized adenovirus for potent knockdown of TRIM25

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM25 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM25 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TRIM25 (NM_005082)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TRIM25
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM25

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for TRIM25
		Search LifeMap BioReagents cell lines for TRIM25
		Sirion Biotech Customized stable knockdown cell line services for TRIM25 Sirion Biotech Customized inducible knockdown cell line services for TRIM25 Sirion Biotech Customized stable overexpressing cell line services for TRIM25 Sirion Biotech Customized inducible overexpressing cell line services for TRIM25

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM25

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/10 super-pathways (see all 10) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways

	RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways	1.00			Negative regulators of RIG-I/MDA5 signaling	0.49
	RIG-I-like receptor signaling pathway	0.52

Cytokine Signaling in Immune system

Cytokine Signaling in Immune system

1.00

Interferon Signaling

0.61

Immune System

Immune System

1.00

Innate Immune System

0.46

Negative regulation of RIG-I/MDA5 signaling by TRIAD3A

Negative regulation of RIG-I/MDA5 signaling by TRIAD3A

1.00

Negative regulation of RIG-I/MDA5 signaling by DUBA

0.91

Translocation of Influenza A virus nonstructural protein 1 (NS1A) into the nucleus

ISG15 antiviral mechanism

0.49

Antiviral mechanism by IFN-stimulated genes

0.49

Pathway sources
See GeneCards unified pathways
Show all pathways

2 Cell Signaling Technology (CST) Pathways for TRIM25

	NF-kappaB Signaling
	Protein Stability

5/14 Reactome Pathways for TRIM25 (see all 14)

	NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
	Antiviral mechanism by IFN-stimulated genes
	Cytokine Signaling in Immune system
	RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
	TRAF3-dependent IRF activation pathway

2 Kegg Pathways (Kegg details for TRIM25):

	RIG-I-like receptor signaling pathway
	Influenza A

UniProtKB/Swiss-Prot: TRI25_HUMAN, Q14258

Pathway: Protein modification; protein ubiquitination

TRIM25 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for TRIM25

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/38 Interacting proteins for TRIM25 (Q14258^{1, 3} ENSP00000323889⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 38)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
SFN	P31947³, ENSP00000340989⁴	I2D: score=4 STRING: ENSP00000340989
UBE2L6	O14933³, ENSP00000287156⁴	I2D: score=3 STRING: ENSP00000287156
UBE2D2	P62837³, ENSP00000381717⁴	I2D: score=2 STRING: ENSP00000381717
UBE2D3	P61077³, ENSP00000349722⁴	I2D: score=2 STRING: ENSP00000349722
UBE2N	P61088³, ENSP00000316176⁴	I2D: score=2 STRING: ENSP00000316176

About this table

Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006355	regulation of transcription, DNA-dependent	TAS	8248217
GO:0009615	response to virus	--	--
GO:0019048	virus-host interaction	IEA	--
GO:0032480	negative regulation of type I interferon production	TAS	--
GO:0044419	interspecies interaction between organisms	--	--

TRIM25 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

TRIM25 for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for TRIM25
3 Novoseek chemical compound relationships for TRIM25 gene About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
atpa	71.3	2	17584914 (1), 16872520 (1)
estrogen	62.7	88	7592654 (5), 10781795 (4), 9855000 (3), 16144914 (3) (see all 18)
zinc	37.5	9	7789997 (2), 15178330 (1), 14978097 (1), 10425199 (1)

Search CenterWatch for drugs/clinical trials and news about TRIM25 / TRI25

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for TRIM25 gene:

NM_005082.4

Unigene Cluster for TRIM25:

Tripartite motif containing 25

Hs.528952 [show with all ESTs]

Unigene Representative Sequence: NM_005082

9 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000316881(uc002iut.3 uc010dcj.3) ENST00000573108 ENST00000572021

ENST00000574234 ENST00000570473 ENST00000570749 ENST00000572550 ENST00000574997

ENST00000537230

miRNA Products:		OriGene 3'-UTR Clone: TRIM25 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TRIM25
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate TRIM25 (see all 14):
		hsa-miR-3671 hsa-miR-345 hsa-miR-1283 hsa-miR-520d-5p hsa-miR-340 hsa-miR-544 hsa-miR-298 hsa-miR-2276

		Browse SwitchGear 3'UTR luciferase reporter plasmids

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TRIM25 (see all 7) OriGene shRNA RFP: TRIM25 OriGene siRNA: TRIM25
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TRIM25
		Sirion Biotech Custom design and validation of potent shRNA sequences against TRIM25

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM25 (see all 4) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM25 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TRIM25 (NM_005082)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TRIM25
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM25

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM25 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat TRIM25
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TRIM25
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TRIM25

Additional cDNA sequence:

AB208994.1 AK024597.1 AK310720.1 BC013752.1 BC016924.1 BC038247.2 BC042541.1 BC053605.1

BC106889.2 BC106890.2 BC114339.2 BX538220.1 D21205.1

9 DOTS entries:

DT.100812467 DT.455205 DT.70105444 DT.100711025 DT.120948423 DT.91688982 DT.95210525 DT.401976

DT.75172513

24/332 AceView cDNA sequences (see all 332):

BQ420434 BQ439544 AI687782 BM826940 AI870733 BU838318 CA313579 BM729089

BE326779 BE893188 CB306376 CD364770 BX389565 AI494023 AA252528 AA648761

AI809930 CB127242 AA304355 BM799254 AI744993 BF513164 R88768 AI417069

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for TRIM25 About this scheme

ExUns:	1a	·	1b	^	2	^	3	^	4a	·	4b	^	5a	·	5b	^	6a	·	6b	^	7a	·	7b	^	8	^	9	^	10a	·	10b	·	10c	^	11
SP1:					-														-				-
SP2:
SP3:																			-
SP4:
SP5:

ECgene alternative splicing isoforms for TRIM25

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

TRIM25 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GGAGCACTGT

About this image

TRIM25 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

3 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Gut Tube

Midgut

Midgut Endoderm Cells

Endoderm

Gut Tube

Liver

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See TRIM25 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TRIM25

SOURCE GeneReport for Unigene cluster: Hs.528952

UniProtKB/Swiss-Prot: TRI25_HUMAN, Q14258

Tissue specificity: Ubiquitous

SABiosciences Expression via Pathway-Focused PCR Arrays including TRIM25:

Antiviral Response in human mouse rat

Ubiquitin Ligases in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM25 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat TRIM25
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TRIM25
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TRIM25

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM25

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of chordates.

Orthologs for TRIM25 gene from 3/10 species (see all 10) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	TRIM25¹	tripartite motif containing 25	59.58(n) 49.84(a)		417401 XM_415653.3 XP_415653.3
lizard (Anolis carolinensis)	Reptilia	TRIM25⁶	--	48(a)	1 ↔ 1	GL343198.1(658793-676009)
zebrafish (Danio rerio)	Actinopterygii	BC045450.1²	--	73.88(n)		393144 BC045450.1

ENSEMBL Gene Tree for TRIM25 (if available)
TreeFam Gene Tree for TRIM25 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for TRIM25 gene

TRIM29² TRIM16L² TRIM47² TRIM16² TRIM65² TRIM8²

2 SIMAP similar genes for TRIM25 using alignment to 4 protein entries: TRI25_HUMAN (see all proteins):

TRIM26 TRIM39

TRIM25 for paralogs About GeneDecksing

1 Pseudogenes.org Pseudogene for TRIM25
PGOHUM00000237505

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 17 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs116810612^1,2	C,F	--	54964857(+)	`AGTGAG/AGAAGA`	1	--	ds500¹		1		WA	118
rs115653379^1,2	C,F	--	54964858(+)	`GTGAGG/AAAGAA`	1	--	ds500¹		1		WA	118
rs150239298^1,2		--	54964861(+)	`AGGAAG/TAAAAA`	1	--	ds500¹		0	--	--	--	--
rs138909374^1,2		--	54964948(+)	`AAACTC/TAGGAG`	1	--	ds500¹		0	--	--	--	--
rs2586521^1,2	C,F,O,A,H	--	54964949(+)	`AACTCG/AGGAGG`	1	--	ds500¹		29		NS EA NA WA CSA	2841
rs149481380^1,2		--	54965030(+)	`CCACCA/GCCTCT`	1	--	ds500¹		0	--	--	--	--
rs183550750^1,2		--	54965048(+)	`CACCAA/CCTGGT`	1	--	ds500¹		0	--	--	--	--
rs187480030^1,2		--	54965118(+)	`GGACAC/TAGTCC`	1	--	ds500¹		0	--	--	--	--
rs190666601^1,2		--	54965159(+)	`GCCCCA/CTGTGG`	1	--	ds500¹		0	--	--	--	--
rs145983666^1,2		--	54965162(+)	`CCATGG/TGGGGA`	1	--	ds500¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for TRIM25 (54965270 - 54991409 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for TRIM25
1 CNV: 7166

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TRIM25

DNA2.0 Custom Variant and Variant Library Synthesis for TRIM25

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

TRIM25 for disorders           About GeneDecksing

OMIM gene information: 600453    OMIM disorders: --

8 diseases for TRIM25:    About MalaCards

breast cancer epithelial ovarian cancer ovarian cancer endometrial carcinoma

influenza carcinoma prostate cancer prostatitis

3 Novoseek disease relationships for TRIM25 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
breast cancer	34	13	16144914 (3), 10781795 (2), 15781984 (2), 12943693 (1)
breast carcinoma	17.2	7	16144914 (6), 15644556 (1)
tumors	6.33	8	12943693 (4), 10781795 (1), 9855000 (1), 16144914 (1)

Human Genome Epidemiology (HuGE) Navigator: TRIM25 (1 document)

Export disorders for TRIM25 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for TRIM25 gene, integrated from 9 sources (see all 75):
(articles sorted by number of sources associating them with TRIM25)

Utopia: connect your pdf to the dynamic
world of online information

Chromosome mapping of human (ZNF147) and mouse genes for estrogen-responsive finger protein (efp), a member of the RING finger family. (PubMed id 7789997)^{1, 3, 9} Inoue S....Muramatsu M. (1995)
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. (PubMed id 8248217)^{1, 2, 9} Inoue S.... Muramatsu M. (1993)
Systemic distribution of estrogen-responsive finger protein (Efp) in human tissues. (PubMed id 15130519)^{1, 2, 9} Shimada N.... Sasano H. (2004)
Influenza A virus NS1 targets the ubiquitin ligase TRIM25 to evade recognition by the host viral RNA sensor RIG-I. (PubMed id 19454348)^{1, 2} Gack M.U.... Garcia-Sastre A. (2009)
Negative regulation of ISG15 E3 ligase EFP through its autoISGylation. (PubMed id 17222803)^{1, 2} Zou W.... Zhang D.-E. (2007)
TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. (PubMed id 17392790)^{1, 2} Gack M.U....Jung J.U. (2007)
A ubiquitin E3 ligase Efp is up-regulated by interferons and conjugated with ISG15. (PubMed id 17069755)^{1, 2} Nakasato N.... Inoue S. (2006)
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. (PubMed id 15592455)^{1, 2} Rush J.... Comb M.J. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Estrogen-responsive finger protein as a new potential biomarker for breast cancer. (PubMed id 16144914)^{1, 9} Suzuki T....Inoue S. (2005)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 7706	HGNC: 12932	AceView: TRIM25	Ensembl:ENSG00000121060	euGenes: HUgn7706
ECgene: TRIM25	Kegg: 7706	H-InvDB: TRIM25

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for TRIM25	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for TRIM25	Genetics and Cytogenetics in Oncology and Haematology
Wikipedia	http://en.wikipedia.org/wiki/TRIM25

Intellectual Property for TRIM25 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for TRIM25 gene:
Search GeneIP for patents involving TRIM25

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Browse Recombinant/Natural Proteins

	OriGene Antibodies for TRIM25		OriGene shRNA RFP for TRIM25
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TRIM25		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TRIM25
	OriGene Protein Over-expression Lysate for TRIM25		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for TRIM25		OriGene 3'-UTR Clone for TRIM25
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM25		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TRIM25
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	OriGene Purified Protein for TRIM25		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for TRIM25		OriGene Custom Protein Services for TRIM25
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat TRIM25
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TRIM25
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TRIM25
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TRIM25
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TRIM25
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TRIM25

	GenScript Custom Purified and Recombinant Proteins Services for TRIM25		GenScript cDNA clones with any tag delivered in your preferred vector for TRIM25
	GenScript Custom Assay Services for TRIM25		GenScript Custom Superior Antibodies Services for TRIM25
	GenScript Custom overexpressing Cell Line Services for TRIM25		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Antibodies & Assays for TRIM25

	Regulatory tfbs in TRIM25 promoter
	Search Chromatin IP Primers for TRIM25
	RT² qPCR Primer Assay in human, mouse, rat TRIM25
	GNC Network for TRIM25
	SABiosciences PCR Arrays including human, mouse, rat TRIM25

Search Tocris compounds for TRIM25

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	TRIM25 antibodies
	TRIM25 proteins
	TRIM25 lysates

	Antibodies for TRIM25
	See all of Abcam's Antibodies, Kits and Proteins for TRIM25
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

TRIM25 Proteins, Antibodies, CLIAs, and ELISAs

Search LifeMap BioReagents cell lines for TRIM25

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TRIM25

	Browse SwitchGear 3'UTR luciferase reporter plasmids for TRIM25
	SwitchGear Promoter luciferase reporter plasmids for TRIM25

ThermoFisher Antibodies for TRIM25

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TRIM25

inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for TRIM25

Sirion Biotech Customized:

	stable knockdown cell line services for TRIM25
	inducible knockdown cell line services for TRIM25
	design and validation of potent shRNA sequences against TRIM25
	adenovirus for potent knockdown of TRIM25
	adenovirus for overexpression of TRIM25
	stable overexpressing cell line services for TRIM25
	inducible overexpressing cell line services for TRIM25

GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 14 May 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes TRIM25 gene at Home site.

hostname: 356977-web1.xennexinc.com index build: 106 solr: 1.4

Advanced Search

Export list of displayed genes

TRIM25 Gene

tripartite motif containing 25

Aliases
for TRIM25 gene

Summaries
for TRIM25 gene

Genomic Views
for TRIM25 gene

Proteins
for TRIM25 gene

Protein Domains /
Families
for TRIM25 gene

Function
for TRIM25 gene

Pathways & Interactions
for TRIM25 gene

Drugs & Compounds
for TRIM25 gene

Transcripts
for TRIM25 gene

Expression
for TRIM25 gene

Orthologs
for TRIM25 gene

Paralogs
for TRIM25 gene

Genomic Variants
for TRIM25 gene

Disorders / Diseases
for TRIM25 gene

Publications
for TRIM25 gene

External Searches for TRIM25 gene

Genome Databases showing TRIM25 gene

Other Databases showing TRIM25 gene

Specialized Databases showing TRIM25 gene

Intellectual Property
for TRIM25 gene

Products
for TRIM25 gene

View Random Gene

Advanced Search

Export list of displayed genes

TRIM25 Gene

tripartite motif containing 25

Aliasesfor TRIM25 gene

Summariesfor TRIM25 gene

Genomic Viewsfor TRIM25 gene

Proteinsfor TRIM25 gene

Protein Domains /Familiesfor TRIM25 gene

Functionfor TRIM25 gene

Pathways & Interactionsfor TRIM25 gene

Drugs & Compoundsfor TRIM25 gene

Transcriptsfor TRIM25 gene

Expression for TRIM25 gene

Orthologsfor TRIM25 gene

Paralogsfor TRIM25 gene

Genomic Variantsfor TRIM25 gene

Disorders / Diseasesfor TRIM25 gene

Publicationsfor TRIM25 gene

External Searches for TRIM25 gene

Genome Databases showing TRIM25 gene

Other Databases showing TRIM25 gene

Specialized Databases showing TRIM25 gene

Intellectual Propertyfor TRIM25 gene

Productsfor TRIM25 gene

View Random Gene

Aliases
for TRIM25 gene

Summaries
for TRIM25 gene

Genomic Views
for TRIM25 gene

Proteins
for TRIM25 gene

Protein Domains /
Families
for TRIM25 gene

Function
for TRIM25 gene

Pathways & Interactions
for TRIM25 gene

Drugs & Compounds
for TRIM25 gene

Transcripts
for TRIM25 gene

Expression
for TRIM25 gene

Orthologs
for TRIM25 gene

Paralogs
for TRIM25 gene

Genomic Variants
for TRIM25 gene

Disorders / Diseases
for TRIM25 gene

Publications
for TRIM25 gene

Intellectual Property
for TRIM25 gene

Products
for TRIM25 gene