TPPP Gene
protein-coding GIFtS: 56
GCID: GC05M000659
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|
tubulin polymerization promoting protein
| |
Aliases
for TPPP gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Tubulin Polymerization Promoting Protein1 2 | | P25-Alpha1 | | TPPP/P251 | | Brain Specific Protein P25 Alpha2 | | TPPP11 2 3 | | Glycogen Synthase Kinase 3 (GSK3) Inhibitor P242 | | P255 | | Tubulin Polymerization-Promoting Protein2 | | P25alpha1 | | P245 | | 25 KDa Brain-Specific Protein2 3 | | P255 | | P245 | | |
Export aliases for TPPP gene to outside databasesPrevious GC identifers: GC05M000713 GC05M000714 |
Summaries
for TPPP gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: TPPP_HUMAN, O94811Function: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and thestabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role inmitotic spindle assembly and nuclear envelope breakdown
Gene Wiki entry for TPPP
|
Genomic Views
for TPPP gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000005.9 NC_018916.1 NT_006576.16
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for TPPP
Other transcription factors
Search SABiosciences Chromatin IP Primers for TPPP
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TPPP |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 5p15.3 Ensembl cytogenetic band: 5p15.33 HGNC cytogenetic band: 5p15.33TPPP Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 5 GeneLoc Exon Structure GeneLoc location for GC05M000659: view genomic region
(about GC identifiers)
Start:
|
659,977 bp from pter |
End:
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693,510 bp from pter |
Size:
|
33,534 bases |
Orientation:
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minus strand |
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Proteins
for TPPP gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: TPPP_HUMAN, O94811 (See
protein sequence)Recommended Name: Tubulin polymerization-promoting protein Size: 219 amino acids; 23694 Da
Subunit: Homodimer. Binds tubulin; binding is inhibited by GTP (By similarity). Interacts with GSK3 (By similarity).Interacts with MAPK1 (By similarity). Interacts with GAPDH; the interaction is direct (By similarity). Interacts withLIMK1 (via the PDZ domain); the interaction is direct
Subcellular location: Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Note=Localizes to glial Lewy bodies in the brains ofindividuals with synucleinopathies
Miscellaneous: Unstructured protein. Interacts with aggregated SNCA and may have a pro-aggregatory role insynucleinopathies
Sequence caution: Sequence=AAH40496.1; Type=Erroneous initiation;Explore the universe of human proteins at neXtProt for TPPP: NX_O94811
Post-translational modifications:
Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter thetubulin polymerization activity1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O94811
TPPP Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_008961.1
ENSEMBL proteins: ENSP00000353785
Human Recombinant Protein Products:
Gene Ontology (GO): 5/7 cellular component terms (GO ID links to tree view) (see all 7): About this table
TPPP for ontologies About GeneDecksing
TPPP Antibody Products:
Assay Products for TPPP: |
Protein
Domains /
Families
for TPPP gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TPPP for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry O94811ProtoNet protein and cluster: O94811 1 Blocks protein family: IPB008907 P25-alpha
UniProtKB/Swiss-Prot: TPPP_HUMAN, O94811Similarity: Belongs to the TPPP family |
Function
for TPPP gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: TPPP_HUMAN, O94811Function: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and thestabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role inmitotic spindle assembly and nuclear envelope breakdown
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TPPP (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TPPP
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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| |  | GenScript: all cDNA clones in your preferred vector: TPPP (NM_007030) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TPPP | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TPPP |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TPPP |
Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view): About this table
TPPP for ontologies About GeneDecksing
|
Pathways & Interactions
for TPPP gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Glial Cell Differentiation | | | 2 | SIDS Susceptibility Pathways | |
2  BioSystems Pathways for TPPP
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TPPP
STRING Interaction
Network Preview (showing 5 interactants - click image to see 6)
5/8 Interacting proteins for TPPP (O948112, 3 ENSP000003537854) via UniProtKB, MINT, STRING, and/or I2D (see all 8)About this table
Gene Ontology (GO): 4 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0001578 | microtubule bundle formation |
ISS | -- | | GO:0031334 | positive regulation of protein complex assembly |
ISS | -- | | GO:0032273 | positive regulation of protein polymerization |
IDA | 15590652 | | GO:0046785 | microtubule polymerization |
ISS | -- |
TPPP for ontologies About GeneDecksing
|
Drugs & Compounds
for TPPP gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for TPPP
1 HMDB Compound for TPPP About this table
Search CenterWatch for drugs/clinical trials and news about TPPP
|
Transcripts
for TPPP gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for TPPP gene: NM_007030.2 Unigene Cluster for TPPP: Tubulin polymerization promoting protein Hs.481466 [show with all ESTs]Unigene Representative Sequence: NM_0070301 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000360578(uc003jbg.4 uc003jbh.4)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TPPP (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TPPP
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: TPPP (NM_007030) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TPPP | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TPPP |
Additional cDNA sequence: AB017016.1 AK054920.1 AK098392.1 AY319261.1 BC040496.1 BC131506.1 BC152382.1 BT020035.1 5 DOTS entries: DT.92011747 DT.417052 DT.97781654 DT.91744550 DT.120853898 24/64 AceView cDNA sequences (see all 64): AA325476 CK904792 AA326143 AA323815 AB017016 BQ066916 BM714107 NM_007030 AK098392 BM686392 BM672396 BU787698 AL120185 BM920759 BF959644 AI083698 BC040496 BX104366 BM694567 BF529144 BE871079 AK054920 AA126498 AL120632
GeneLoc Exon Structure
|
Expression
for TPPP gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TPPP expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GCTGTTCTGA
About this image
See TPPP Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TPPP
SOURCE GeneReport for Unigene cluster: Hs.481466
UniProtKB/Swiss-Prot: TPPP_HUMAN, O94811Tissue specificity: Widely expressed
SABiosciences Custom PCR Arrays for TPPP
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TPPP
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat TPPP | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TPPP | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TPPP | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TPPP |
Orthologs
for TPPP gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for TPPP gene from 6/21 species (see all 21) About this table
ENSEMBL Gene Tree for TPPP (if available)
TreeFam Gene Tree for TPPP (if available) |
Paralogs
for TPPP gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for TPPP gene
- TPPP32 TPPP22
3 SIMAP similar genes for TPPP using alignment to 1 protein entry: TPPP_HUMAN:FREP1 TPPP3 TPPP2
TPPP for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for TPPP
PGOHUM00000235108
|
Genomic Variants
for TPPP gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 5 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for TPPP (659977 - 693510 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 40 variations for TPPP
15/32 CNVs (see all 32): 0705 80764 80761 10086 92796 51735 68944 2079 38968 7503 80763 99033 0704 31218 32692
8 Indels: 27996 68943 12637 59271 92799 27995 92795 46915 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TPPP |
|
Disorders
/ Diseases
for TPPP gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TPPP for disorders About GeneDecksing
OMIM gene information: 608773
OMIM disorders: --
13  diseases for TPPP: About MalaCardslewy body dementia lymphoid interstitial pneumonia multiple system atrophy multiple myeloma
multiple sclerosis dementia myeloma alzheimer's disease
neurodegeneration pneumonia cerebritis malaria
neuronitis
2 diseases from the University of Copenhagen DISEASES database for TPPP:Multiple system atrophy Lewy body dementia Export disorders for TPPP gene to outside databases
|
Publications
for TPPP gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TPPP gene, integrated from 9 sources (see all 51):
(articles sorted by number of sources associating them with TPPP) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Tubulin polymerization promoting proteins (TPPPs): members of a new family with distinct structures and functions. (PubMed id 17105200)1, 2, 3 Vincze O....Ovadi J. (2006)
- p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies. (PubMed id 15590652)1, 2, 3 Lindersson E....Jensen P.H. (2005)
- A novel human gene whose product shares significant homology with the bovine brain-specific protein p25 on chromosome 5p15.3. (PubMed id 10083737)1, 2, 3 Seki N.... Saito T. (1999)
- Phosphorylation blocks the activity of tubulin polymerization-promoting protein (TPPP): identification of sites targeted by different kinases. (PubMed id 17693641)1, 2, 9 Hlavanda E....Ovadi J. (2007)
- The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules. (PubMed id 18028908)1, 2 Acevedo K....Bernard O. (2007)
- Interaction of TPPP/p25 protein with glyceraldehyde-3-phosphate dehydrogenase and their co-localization in Lewy bodies. (PubMed id 17027006)1, 2 Olah J....Ovadi J. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Brain-specific p25 protein binds to tubulin and microtubules and induces aberrant microtubule assemblies at substoichiometric concentrations. (PubMed id 12093283)1, 3 Hlavanda E....Ovadi J. (2002)
- p25alpha relocalizes in oligodendroglia from myelin to cytoplasmic inclusions in multiple system atrophy. (PubMed id 17823288)1, 9 Song Y.J....Jensen P.H. (2007)
- Gain of 5p15.33 is associated with progression of bladder cancer. (PubMed id 18025801)1, 9 Yamamoto Y....Sasaki K. (2007)
|
External Searches for TPPP gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing TPPP gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TPPP gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TPPP gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for TPPP | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for TPPP gene:
Search GeneIP for patents involving TPPP
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for TPPP gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | OriGene Antibodies for TPPP | | OriGene shRNA RFP for TPPP | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TPPP | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TPPP | | | OriGene Protein Over-expression Lysate for TPPP | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for TPPP | | OriGene 3'-UTR Clone for TPPP | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TPPP | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TPPP | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for TPPP | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for TPPP | | OriGene Custom Protein Services for TPPP | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat TPPP | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TPPP | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TPPP | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TPPP | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TPPP | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TPPP |
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 | | | TPPP Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TPPP |
|  |  |  | | | | ThermoFisher Antibodies for TPPP |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TPPP |
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