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TOP3A Gene

protein-coding   GIFtS: 62
GCID: GC17M018175

Topoisomerase (DNA) III Alpha


(Previous symbol: TOP3)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Topoisomerase (DNA) III Alpha1 2     ZGRF72
TOP31 2 3     DNA Topoisomerase 3-Alpha2
DNA Topoisomerase III Alpha2 3     Topo III-Alpha2
GRF-Type Containing 71     Zinc Finger, GRF-Type Containing 72
Zinc Finger1     EC 5.99.1.23

External Ids:    HGNC: 119921   Entrez Gene: 71562   Ensembl: ENSG000001773027   OMIM: 6012435   UniProtKB: Q134723   

Export aliases for TOP3A gene to outside databases

Previous GC identifers: GC17M018422 GC17M019769 GC17M018117 GC17M018377 GC17M017930


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for TOP3A Gene:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during
transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows
the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA.
This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells.
(provided by RefSeq, Jul 2008)

GeneCards Summary for TOP3A Gene:
TOP3A (topoisomerase (DNA) III alpha) is a protein-coding gene. Diseases associated with TOP3A include rothmund-thomson syndrome, and fanconi anemia, complementation group m. GO annotations related to this gene include DNA topoisomerase type I activity. An important paralog of this gene is TOP3B.

UniProtKB/Swiss-Prot: TOP3A_HUMAN, Q13472
Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and
transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand
break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the
catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and
the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus
removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to
expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI
complex, a complex that plays an important role in the processing of homologous recombination intermediates to
limit DNA crossover formation in cells. Has DNA decatenation activity

Gene Wiki entry for TOP3A Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000017.11  NT_010718.17  NC_018928.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the TOP3A gene promoter:
         AREB6   Elk-1   STAT1   Pax-2b   Pax-2   STAT1beta   Pax-2a   STAT1alpha   YY1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTOP3A promoter sequence
   Search Chromatin IP Primers for TOP3A

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TOP3A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17p12-p11.2   Ensembl cytogenetic band:  17p11.2   HGNC cytogenetic band: 17p12-p11.2

TOP3A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TOP3A gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M018175:  view genomic region     (about GC identifiers)

Start:
18,174,742 bp from pter      End:
18,218,321 bp from pter
Size:
43,580 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: TOP3A_HUMAN, Q13472 (See protein sequence)
Recommended Name: DNA topoisomerase 3-alpha  
Size: 1001 amino acids; 112372 Da
Cofactor: Magnesium
Subunit: Interacts (via N-terminus region) with BLM; the interaction is direct. Directly interacts with RMI1.
Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM
Secondary accessions: A8KA61 D3DXC7 Q13473
Alternative splicing: 2 isoforms:  Q13472-1   Q13472-2   

Explore the universe of human proteins at neXtProt for TOP3A: NX_Q13472

Explore proteomics data for TOP3A at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys257, Lys557
  • Modification sites at PhosphoSitePlus

  • See TOP3A Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_004609.1  
    ENSEMBL proteins: 
     ENSP00000465014   ENSP00000465864   ENSP00000321636   ENSP00000462136   ENSP00000462378  
     ENSP00000462790   ENSP00000466099   ENSP00000466641   ENSP00000464338   ENSP00000464493  
     ENSP00000442336   ENSP00000446425  
    Reactome Protein details: Q13472

    TOP3A Human Recombinant Protein Products:

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    OriGene Custom Protein Services for TOP3A
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    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

    TOP3A Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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    OriGene Custom Antibody Services for TOP3A
    Abcam antibodies for TOP3A
    Browse Antibodies at Cloud-Clone Corp.
    Search ThermoFisher Antibodies for TOP3A
    Search LSBio for Antibodies for TOP3A

    TOP3A Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for TOP3A
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 12):
     IPR013498 Topo_IA_Znf
     IPR000380 Topo_IA
     IPR003601 Topo_IA_2
     IPR023406 Topo_IA_AS
     IPR003602 Topo_IA_DNA-bd

    Graphical View of Domain Structure for InterPro Entry Q13472

    ProtoNet protein and cluster: Q13472

    4 Blocks protein domains:
    IPB000380 DNA topoisomerase I
    IPB001878 C2HC-type zinc-finger signature
    IPB006154 Toprim sub-domain
    IPB010666 GRF zinc finger


    UniProtKB/Swiss-Prot: TOP3A_HUMAN, Q13472
    Similarity: Belongs to the type IA topoisomerase family
    Similarity: Contains 1 Toprim domain


    TOP3A for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: TOP3A_HUMAN, Q13472
    Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and
    transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand
    break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the
    catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and
    the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus
    removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to
    expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI
    complex, a complex that plays an important role in the processing of homologous recombination intermediates to
    limit DNA crossover formation in cells. Has DNA decatenation activity
    Catalytic activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining

         Genatlas biochemistry entry for TOP3A:
    DNA topoisomerase III,putatively involved in suppression of mitotic recombination between repetitive
    sequences,deleted in SMCR

         Enzyme Number (IUBMB): EC 5.99.1.21

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0003916DNA topoisomerase activity ----
    GO:0003917DNA topoisomerase type I activity IDA--
    GO:0005515protein binding IPI10728666
         
    TOP3A for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for TOP3A:
     Cells with protrusions 

         3 MGI mutant phenotypes (inferred from 1 allele(MGI details for Top3a):
     cellular  embryogenesis  mortality/aging 

    TOP3A for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Top3atm1Jcw for TOP3A

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for TOP3A
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for TOP3A

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for TOP3A
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for TOP3A

    miRNA
    Products:
        
    miRTarBase miRNAs that target TOP3A:
    hsa-mir-222-3p (MIRT046639), hsa-mir-320a (MIRT044415)

    Block miRNA regulation of human, mouse, rat TOP3A using miScript Target Protectors
    Search for qRT-PCR Assays for microRNAs that regulate TOP3A
    SwitchGear 3'UTR luciferase reporter plasmidTOP3A 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for TOP3A
    Predesigned siRNA for gene silencing in human, mouse, rat TOP3A

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for TOP3A

    Clone
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    OriGene clones in human, mouse for TOP3A (see all 6)
    OriGene ORF clones in mouse, rat for TOP3A
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: TOP3A (NM_004618)
    Sino Biological Human cDNA Clone for TOP3A
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP3A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP3A

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for TOP3A 
    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP3A


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2
    mitochondrion2

    Gene Ontology (GO): 3 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus TAS8622991
    GO:0005694chromosome IEA--
    GO:0016605PML body IDA10728666

    TOP3A for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for TOP3A About    
    See pathways by source

    SuperPathContained pathways About
    1Meiosis (REACTOME)
    Meiosis0.74
    Meiotic Recombination0.74
    2Homologous recombination
    Homologous recombination0.40
    3Meiosis (EMD)
    Meiosis
    4Fanconi anemia pathway (KEGG)
    Fanconi anemia pathway

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways



    1 Reactome Pathway for TOP3A
        Meiotic recombination


    2 Kegg Pathways  (Kegg details for TOP3A):
        Homologous recombination
    Fanconi anemia pathway


    TOP3A for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Array including TOP3A: 
              DNA Repair in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for TOP3A

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for TOP3A (Q134722, 3 ENSP000003216364) via UniProtKB, MINT, STRING, and/or I2D (see all 230)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    BLMP541322, 3, ENSP000003472324MINT-8048980 MINT-8049128 MINT-8048995 MINT-8049010 MINT-8415512 MINT-8048961 MINT-8049038 I2D: score=5 STRING: ENSP00000347232
    TERF2Q155542, 3, ENSP000002549424MINT-6743829 MINT-6744175 MINT-6744122 MINT-6743896 MINT-6744078 I2D: score=1 STRING: ENSP00000254942
    PMLP295902, 3, ENSP000002680584MINT-6743913 MINT-6743884 MINT-6743926 MINT-6744122 I2D: score=1 STRING: ENSP00000268058
    PLK1P533502, 3, ENSP000003000934MINT-8415512 I2D: score=1 STRING: ENSP00000300093
    RMI1Q9H9A72, 3, ENSP000003170394MINT-8048980 MINT-8049128 MINT-8048995 MINT-8049010 MINT-8048961 MINT-8049038 I2D: score=2 STRING: ENSP00000317039
    About this table

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006265DNA topological change IDA--
    GO:0007126meiotic nuclear division TAS8622991

    TOP3A for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for TOP3A

    2 Novoseek inferred chemical compound relationships for TOP3A gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    methylmethanesulfonate 69.1 2 15590327 (1)
    hydroxyurea 56.9 2 11585898 (1)



    TOP3A for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for TOP3A gene: 
    NM_004618.3  

    Unigene Cluster for TOP3A:

    Topoisomerase (DNA) III alpha
    Hs.592115  [show with all ESTs]
    Unigene Representative Sequence: NM_004618
    Selected Ensembl transcripts including schematic representations, and UCSC links where relevant (see all 21):
    ENST00000493648 ENST00000489131 ENST00000469739(uc002gsw.1) ENST00000321105(uc010cpz.1 uc010vxr.1 uc002gsx.1 uc010vxs.1)
    ENST00000584582 ENST00000582981(uc010cqa.1) ENST00000580095 ENST00000477508
    ENST00000582122 ENST00000581536 ENST00000583804 ENST00000461127 ENST00000584669
    ENST00000583328 ENST00000472959 ENST00000585031 ENST00000582230 ENST00000584887

    miRNA
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    Block miRNA regulation of human, mouse, rat TOP3A using miScript Target Protectors
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    SwitchGear 3'UTR luciferase reporter plasmidTOP3A 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat TOP3A
    Clone
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    OriGene ORF clones in mouse, rat for TOP3A
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: TOP3A (NM_004618)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP3A
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP3A
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for TOP3A
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat TOP3A
      QuantiTect SYBR Green Assays in human, mouse, rat TOP3A
      QuantiFast Probe-based Assays in human, mouse, rat TOP3A

    Additional mRNA sequence: 

    AF086042.1 AK126869.1 AK290513.1 AK292926.1 AK296437.1 AK299764.1 AK311668.1 BC051748.1 
    U43431.1 

    10 DOTS entries:

    DT.309126  DT.95373118  DT.95132204  DT.97827020  DT.120950465  DT.100754182  DT.120950491  DT.100023593 
    DT.100754179  DT.91856693 

    Selected AceView cDNA sequences (see all 135):

    BM817686 AA307047 AW139468 CR593799 U43431 BQ673046 BP381656 AI969044 
    BP350268 BX348607 AW449041 AA336145 AI968390 BM046548 NM_004618 BQ215187 
    AU130137 BM793025 BU191611 BU902730 AW513442 BM910465 BC051748 BU542042 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for TOP3A (see all 11)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^
    SP1:                                      -     -     -           -           -                                                                                 
    SP2:                                                              -           -                                                     -     -     -               
    SP3:                                            -     -           -                                                                                             
    SP4:                          -     -     -     -     -           -           -                                                                                 
    SP5:                                      -     -     -           -     -     -                                                                                 

    ExUns: 17a · 17b ^ 18a · 18b ^ 19 ^ 20a · 20b
    SP1:                    -                     
    SP2:                    -                     
    SP3:                                          
    SP4:                                          
    SP5:                                          


    ECgene alternative splicing isoforms for TOP3A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    TOP3A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GGGCAGTCCC
    TOP3A Expression
    About this image


    TOP3A expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 5) fully expand
     
     Ovary (Reproductive System)
             Oviduct
     
     Pancreas (Endocrine System)
             Islets of Langerhans
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Brain (Nervous System)
             Cerebral Cortex
    TOP3A Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    TOP3A Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.592115

    UniProtKB/Swiss-Prot: TOP3A_HUMAN, Q13472
    Tissue specificity: High expression is found in testis, heart, skeletal muscle and pancreas

        Pathway & Disease-focused RT2 Profiler PCR Array including TOP3A: 
              DNA Repair in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for TOP3A
    OriGene qSTAR qPCR primer pairs in human, mouse for TOP3A
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat TOP3A
    QuantiTect SYBR Green Assays in human, mouse, rat TOP3A
    QuantiFast Probe-based Assays in human, mouse, rat TOP3A
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP3A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for TOP3A gene from Selected species (see all 19)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Top3a1 , 5 topoisomerase (DNA) III alpha1, 5 85.18(n)1
    87.01(a)1
      11 (37.81 cM)5
    219751  NM_009410.21  NP_033436.11 
     607400585 
    chicken
    (Gallus gallus)
    Aves TOP3A1 topoisomerase (DNA) III alpha 71.1(n)
    74.9(a)
      416517  NM_001030636.2  NP_001025807.1 
    lizard
    (Anolis carolinensis)
    Reptilia TOP3A6
    topoisomerase (DNA) III alpha
    72(a)
    1 ↔ 1
    GL343618.1(177099-191446)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.89422 Xenopus laevis transcribed sequence with weak similarity more 81.66(n)    BQ386610.1 
    zebrafish
    (Danio rerio)
    Actinopterygii top3a1 topoisomerase (DNA) III alpha 64.78(n)
    68.38(a)
      560212  XM_683603.6  XP_688695.4 
    fruit fly
    (Drosophila melanogaster)
    Insecta Top3alpha1 , 3 DNA topoisomerase I3
    Topoisomerase 3alpha1
    58(a)3
    56.23(n)1
    55.92(a)1
      37E43
    352361  NM_078878.31  NP_523602.21 
    worm
    (Caenorhabditis elegans)
    Secernentea top-31 , 3 DNA topoisomerase III3
    top-31
    51(a)3
    53.79(n)1
    52.96(a)1
      III(12021663-12031813)3
    1766311  NM_067157.41  NP_499558.11 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes TOP36
    DNA Topoisomerase III, conserved protein that func...
    40(a)
    1 → many
    XII(609783-611753) YLR234W
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons TOP3A1 TOP3A 51.86(n)
    45.66(a)
      836513  NM_125788.6  NP_201197.1 
    rice
    (Oryza sativa)
    Liliopsida Os03g01650001 Os03g0165000 51.87(n)
    45.51(a)
      9269718  NM_001186349.1  NP_001173278.1 


    ENSEMBL Gene Tree for TOP3A (if available)
    TreeFam Gene Tree for TOP3A (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

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    Paralogs for TOP3A gene
    TOP3B2  
    1 SIMAP similar gene for TOP3A using alignment to 11 protein entries:     TOP3A_HUMAN (see all proteins):
    TOP3B

    TOP3A for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

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    Selected SNPs for TOP3A (see all 911)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1166870371,2
    C,F--18176846(+) AGGGTG/AAGCTC 1 -- ds50011Minor allele frequency- A:0.03WA 118
    rs72159691,2
    C,F,H--18176849(+) GTGAGC/TTCCTT 1 -- ds50017Minor allele frequency- T:0.01NS EA NA WA 646
    rs1919816781,2
    --18177064(+) TCAGCC/TTCCTG 1 -- ds50010--------
    rs1845937901,2
    --18177087(+) AAAGAC/TGTGTA 1 -- ds50010--------
    rs80780151,2
    C,F,H--18177089(+) agacgT/Cgtaca 1 -- ds500116Minor allele frequency- C:0.05NS EA NA WA 1922
    rs1897318041,2
    --18177224(+) GAGCCC/TCCACG 1 -- ds50010--------
    rs1813676941,2
    --18177270(+) TTTTTA/GAATTT 1 -- ut310--------
    rs1178100731,2
    F--18177312(+) GTGGCG/ACAATC 1 -- ut311Minor allele frequency- A:0.02NA 120
    rs1488226851,2
    --18177347(+) CCGGGC/TTCAAG 1 -- ut310--------
    rs1434471041,2
    --18177493(+) CAATCC/TGCCCC 1 -- ut310--------

    HapMap Linkage Disequilibrium report for TOP3A (18174742 - 18218321 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 9 variations for TOP3A:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2715721CNV Deletion23290073
    esv2657654CNV Deletion23128226
    esv1743367CNV Insertion17803354
    dgv3078n71CNV Loss21882294
    nsv833387CNV Loss17160897
    nsv428333CNV Gain18775914
    nsv833386CNV Gain17160897
    nsv155OTHER Inversion15895083
    nsv499114OTHER Inversion21111241

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing TOP3A
    DNA2.0 Custom Variant and Variant Library Synthesis for TOP3A

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 601243    OMIM disorders: --

    Selected diseases for TOP3A (see all 24):    
    About MalaCards
    rothmund-thomson syndrome    fanconi anemia, complementation group m    appendicitis    bloom syndrome
    werner syndrome    mitochondrial disorders    smith magenis syndrome    fanconi's anemia
    albinism    vaccinia    ataxia telangiectasia    hypoxia
    ataxia    oral squamous cell carcinoma    tuberculosis    tonsillitis
    squamous cell carcinoma    malaria    thyroiditis    cerebritis

    4 diseases from the University of Copenhagen DISEASES database for TOP3A:
    Vaccinia     Bloom syndrome     Werner syndrome     Cancer

    TOP3A for disorders           About GeneDecksing

    1 Novoseek inferred disease relationship for TOP3A gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tumors 0 13 17563752 (2), 17483326 (1), 16324386 (1)

    Genetic Association Database (GAD): TOP3A
    Human Genome Epidemiology (HuGE) Navigator: TOP3A (1 document)

    Export disorders for TOP3A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

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    PubMed articles for TOP3A gene, integrated from 10 sources (see all 77):
    (articles sorted by number of sources associating them with TOP3A)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human TOP3: a single-copy gene encoding DNA topoisomerase III. (PubMed id 8622991)1, 2, 9 Hanai R.... Wang J.C. (Proc. Natl. Acad. Sci. U.S.A. 1996)
    2. Identification of rare DNA variants in mitochondrial disorders with improved array-based sequencing. (PubMed id 20843780)1, 2 Wang W.... Scharfe C. (Nucleic Acids Res. 2011)
    3. Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. (PubMed id 20445207)1, 2 Yang J.... Brown G.W. (J. Biol. Chem. 2010)
    4. Association between polymorphisms in RMI1, TOP3A, and BLM and risk of cancer, a case-control study. (PubMed id 19432957)1, 4 Broberg K....HAPglund M. (BMC Cancer 2009)
    5. BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity. (PubMed id 15775963)1, 2 Yin J.... Wang W. (EMBO J. 2005)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    7. Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III I+-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis. (PubMed id 24108125)1 Pradhan A....Meetei A.R. (J. Biol. Chem. 2013)
    8. TopBP1 controls BLM protein level to maintain genome stability. (PubMed id 24239288)1 Wang J....Gong Z. (Mol. Cell 2013)
    9. Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair. (PubMed id 23509288)2 Wan L....Huang J. (Proc. Natl. Acad. Sci. U.S.A. 2013)
    10. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (Cell 2012)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

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    Entrez Gene: 7156 HGNC: 11992 AceView: TOP3A Ensembl:ENSG00000177302 euGenes: HUgn7156
    ECgene: TOP3A Kegg: 7156 H-InvDB: TOP3A

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for TOP3A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for TOP3A gene:
    Search GeneIP for patents involving TOP3A

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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