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Aliases for TOP3A Gene

Aliases for TOP3A Gene

  • Topoisomerase (DNA) III Alpha 2 3
  • DNA Topoisomerase III Alpha 3 4
  • TOP3 3 4
  • Zinc Finger, GRF-Type Containing 7 3
  • GRF-Type Containing 7 2
  • Topo III-Alpha 3
  • Zinc Finger 2
  • EC 5.99.1.2 4
  • ZGRF7 3

External Ids for TOP3A Gene

Previous HGNC Symbols for TOP3A Gene

  • TOP3

Previous GeneCards Identifiers for TOP3A Gene

  • GC17M018422
  • GC17M019769
  • GC17M018117
  • GC17M018377
  • GC17M017930
  • GC17M018175

Summaries for TOP3A Gene

Entrez Gene Summary for TOP3A Gene

  • This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. [provided by RefSeq, Jul 2008]

GeneCards Summary for TOP3A Gene

TOP3A (Topoisomerase (DNA) III Alpha) is a Protein Coding gene. Diseases associated with TOP3A include occupational dermatitis. Among its related pathways are Cell Cycle, Mitotic and Homologous recombination. GO annotations related to this gene include nucleic acid binding and DNA topoisomerase activity. An important paralog of this gene is TOP3B.

UniProtKB/Swiss-Prot for TOP3A Gene

  • Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

Gene Wiki entry for TOP3A Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TOP3A Gene

Genomics for TOP3A Gene

Regulatory Elements for TOP3A Gene

Genomic Location for TOP3A Gene

Chromosome:
17
Start:
18,271,428 bp from pter
End:
18,315,007 bp from pter
Size:
43,580 bases
Orientation:
Minus strand

Genomic View for TOP3A Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for TOP3A Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TOP3A Gene

Proteins for TOP3A Gene

  • Protein details for TOP3A Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13472-TOP3A_HUMAN
    Recommended name:
    DNA topoisomerase 3-alpha
    Protein Accession:
    Q13472
    Secondary Accessions:
    • A8KA61
    • B4DK80
    • D3DXC7
    • Q13473

    Protein attributes for TOP3A Gene

    Size:
    1001 amino acids
    Molecular mass:
    112372 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.

    Three dimensional structures from OCA and Proteopedia for TOP3A Gene

    Alternative splice isoforms for TOP3A Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for TOP3A Gene

Proteomics data for TOP3A Gene at MOPED

Post-translational modifications for TOP3A Gene

  • Ubiquitination at Lys 257 and Lys 557
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for TOP3A Gene

Domains & Families for TOP3A Gene

Gene Families for TOP3A Gene

Graphical View of Domain Structure for InterPro Entry

Q13472

UniProtKB/Swiss-Prot:

TOP3A_HUMAN :
  • Contains 1 Toprim domain.
  • Belongs to the type IA topoisomerase family.
Domain:
  • Contains 1 Toprim domain.
Family:
  • Belongs to the type IA topoisomerase family.
genes like me logo Genes that share domains with TOP3A: view

Function for TOP3A Gene

Molecular function for TOP3A Gene

GENATLAS Biochemistry:
DNA topoisomerase III,putatively involved in suppression of mitotic recombination between repetitive sequences,deleted in SMCR
UniProtKB/Swiss-Prot CatalyticActivity:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
UniProtKB/Swiss-Prot Function:
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.

Enzyme Numbers (IUBMB) for TOP3A Gene

Gene Ontology (GO) - Molecular Function for TOP3A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding --
GO:0003677 DNA binding IEA --
GO:0003916 DNA topoisomerase activity --
GO:0003917 DNA topoisomerase type I activity IDA 20445207
GO:0005515 protein binding IPI 10728666
genes like me logo Genes that share ontologies with TOP3A: view
genes like me logo Genes that share phenotypes with TOP3A: view

Animal Models for TOP3A Gene

MGI Knock Outs for TOP3A:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for TOP3A

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for TOP3A Gene

Localization for TOP3A Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for TOP3A Gene COMPARTMENTS Subcellular localization image for TOP3A gene
Compartment Confidence
nucleus 5
cytosol 2
mitochondrion 2

Gene Ontology (GO) - Cellular Components for TOP3A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 8622991
GO:0005654 nucleoplasm TAS --
GO:0005694 chromosome IEA --
GO:0016605 PML body IDA 10728666
genes like me logo Genes that share ontologies with TOP3A: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for TOP3A Gene

Pathways & Interactions for TOP3A Gene

genes like me logo Genes that share pathways with TOP3A: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for TOP3A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000724 double-strand break repair via homologous recombination TAS --
GO:0006265 DNA topological change IDA 20445207
GO:0006281 DNA repair TAS --
GO:0006302 double-strand break repair TAS --
GO:0007126 meiotic nuclear division TAS 8622991
genes like me logo Genes that share ontologies with TOP3A: view

No data available for SIGNOR curated interactions for TOP3A Gene

Drugs & Compounds for TOP3A Gene

(1) Drugs for TOP3A Gene - From: NovoSeek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Hydroxyurea Approved Pharma 181

(1) Additional Compounds for TOP3A Gene - From: NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Methylmethanesulfonate
genes like me logo Genes that share compounds with TOP3A: view

Transcripts for TOP3A Gene

Unigene Clusters for TOP3A Gene

Topoisomerase (DNA) III alpha:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for TOP3A

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for TOP3A Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^
SP1: - - - - -
SP2: - - - - -
SP3: - - -
SP4: - - - - - - -
SP5: - - - - - -
SP6: - - - - - -
SP7: -
SP8:
SP9: - -
SP10:
SP11:

ExUns: 17a · 17b ^ 18a · 18b ^ 19 ^ 20a · 20b
SP1: -
SP2: -
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:

Relevant External Links for TOP3A Gene

GeneLoc Exon Structure for
TOP3A
ECgene alternative splicing isoforms for
TOP3A

Expression for TOP3A Gene

mRNA expression in normal human tissues for TOP3A Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for TOP3A Gene

This gene is overexpressed in Retina (57.3) and Testis (10.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for TOP3A Gene



SOURCE GeneReport for Unigene cluster for TOP3A Gene Hs.592115

mRNA Expression by UniProt/SwissProt for TOP3A Gene

Q13472-TOP3A_HUMAN
Tissue specificity: High expression is found in testis, heart, skeletal muscle and pancreas
genes like me logo Genes that share expression patterns with TOP3A: view

Protein tissue co-expression partners for TOP3A Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for TOP3A Gene

Orthologs for TOP3A Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for TOP3A Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia TOP3A 35
  • 82.93 (n)
  • 85.77 (a)
TOP3A 36
  • 85 (a)
OneToOne
dog
(Canis familiaris)
Mammalia TOP3A 35
  • 87.86 (n)
  • 89.83 (a)
TOP3A 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Top3a 35
  • 85.18 (n)
  • 87.01 (a)
Top3a 16
Top3a 36
  • 87 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia TOP3A 35
  • 99.3 (n)
  • 99.1 (a)
TOP3A 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Top3a 35
  • 83.26 (n)
  • 83.33 (a)
oppossum
(Monodelphis domestica)
Mammalia TOP3A 36
  • 79 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia TOP3A 36
  • 79 (a)
OneToOne
chicken
(Gallus gallus)
Aves TOP3A 35
  • 71.1 (n)
  • 74.9 (a)
TOP3A 36
  • 72 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia TOP3A 36
  • 72 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.19892 35
top3a 35
  • 71.61 (n)
  • 74.54 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.8942 35
zebrafish
(Danio rerio)
Actinopterygii top3a 35
  • 64.78 (n)
  • 68.38 (a)
top3a 36
  • 64 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Top3alpha 37
  • 58 (a)
Top3alpha 35
  • 56.23 (n)
  • 55.92 (a)
Top3alpha 36
  • 39 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010028 35
  • 53.15 (n)
  • 51.02 (a)
worm
(Caenorhabditis elegans)
Secernentea top-3 37
  • 51 (a)
top-3 35
  • 53.79 (n)
  • 52.96 (a)
top-3 36
  • 47 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TOP3 36
  • 40 (a)
OneToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons TOP3A 35
  • 51.86 (n)
  • 45.66 (a)
rice
(Oryza sativa)
Liliopsida Os03g0165000 35
  • 51.87 (n)
  • 45.51 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 53 (a)
OneToOne
Species with no ortholog for TOP3A:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for TOP3A Gene

ENSEMBL:
Gene Tree for TOP3A (if available)
TreeFam:
Gene Tree for TOP3A (if available)

Paralogs for TOP3A Gene

Paralogs for TOP3A Gene

genes like me logo Genes that share paralogs with TOP3A: view

Variants for TOP3A Gene

Sequence variations from dbSNP and Humsavar for TOP3A Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type MAF
rs1925 -- 18,274,401(-) TGGGG(C/T)AGTCC utr-variant-3-prime
rs1001303 -- 18,277,290(-) GATGA(A/G)TACAT intron-variant
rs2061214 -- 18,305,494(-) GCGCG(C/T)GCGCG intron-variant, upstream-variant-2KB
rs2061215 -- 18,305,492(-) GCGCG(C/T)GCGCG intron-variant, upstream-variant-2KB
rs2061217 -- 18,305,488(-) GCGCG(C/T)GTGTG intron-variant, upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for TOP3A Gene

Variant ID Type Subtype PubMed ID
nsv155 OTHER Inversion 15895083
nsv499114 OTHER Inversion 21111241
dgv3078n71 CNV Loss 21882294
nsv833386 CNV Gain 17160897
esv2715721 CNV Deletion 23290073
esv2657654 CNV Deletion 23128226
nsv833387 CNV Loss 17160897
nsv428333 CNV Gain 18775914
esv1743367 CNV Insertion 17803354

Variation tolerance for TOP3A Gene

Residual Variation Intolerance Score: 16.94% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.54; 77.81% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for TOP3A Gene

HapMap Linkage Disequilibrium report
TOP3A

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TOP3A Gene

Disorders for TOP3A Gene

MalaCards: The human disease database

(1) MalaCards diseases for TOP3A Gene - From: DISEASES

Disorder Aliases PubMed IDs
occupational dermatitis
  • occupational allergic contact dermatitis
- elite association
Search TOP3A in MalaCards View complete list of genes associated with diseases

Relevant External Links for TOP3A

Genetic Association Database (GAD)
TOP3A
Human Genome Epidemiology (HuGE) Navigator
TOP3A
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
TOP3A
genes like me logo Genes that share disorders with TOP3A: view

No data available for UniProtKB/Swiss-Prot and Genatlas for TOP3A Gene

Publications for TOP3A Gene

  1. Sgs1 function in the repair of DNA replication intermediates is separable from its role in homologous recombinational repair. (PMID: 19214189) Bernstein K.A. … Rothstein R. (EMBO J. 2009) 23 67
  2. Helicase-appended topoisomerases: new insight into the mechanism of directional strand transfer. (PMID: 19726668) Plank J. … Hsieh T.S. (J. Biol. Chem. 2009) 23 67
  3. Binding and activation of DNA topoisomerase III by the Rmi1 subunit. (PMID: 17693398) Chen C.F. … Brill S.J. (J. Biol. Chem. 2007) 23 67
  4. Hypoxia and hypoxia-inducible factor-1 expression enhance osteolytic bone metastases of breast cancer. (PMID: 17483326) Hiraga T. … Yoneda T. (Cancer Res. 2007) 23 67
  5. Significance of HIF-1-active cells in angiogenesis and radioresistance. (PMID: 17563752) Harada H. … Hiraoka M. (Oncogene 2007) 23 67

Products for TOP3A Gene

Sources for TOP3A Gene

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