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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TOP2B Gene

protein-coding   GIFtS: 64
GCID: GC03M025639

Topoisomerase (DNA) II Beta 180kDa

(Previous name: topoisomerase (DNA) II beta (180kD))
Microbiology & Infectious Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Topoisomerase (DNA) II Beta 180kDa1 2     DNA Topoisomerase II Beta2
DNA Topoisomerase II, Beta Isozyme2 3     DNA Topoisomerase II, 180 KD2
EC 5.99.1.33 8     top2beta2
Topoisomerase (DNA) II Beta (180kD)1     Topo II Beta2
TOPIIB2     Topoisomerase II Beta2
Antigen MLAA-442     Topoisomerase IIb2
DNA Topoisomerase 2-Beta2     U937 Associated Antigen2

External Ids:    HGNC: 119901   Entrez Gene: 71552   Ensembl: ENSG000000770977   OMIM: 1264315   UniProtKB: Q028803   

Export aliases for TOP2B gene to outside databases

Previous GC identifers: GC03M025346 GC03M025489 GC03M025614 GC03M025584


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TOP2B Gene:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during
transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid
separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes
the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one
another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene
duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is
localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents
and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced
activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results
in two transcript variants; however, the second variant has not yet been fully described. (provided by RefSeq,
Jul 2008)

GeneCards Summary for TOP2B Gene: 
TOP2B (topoisomerase (DNA) II beta 180kDa) is a protein-coding gene. Diseases associated with TOP2B include ataxia telangiectasia, and rubella, and among its related super-pathways are Cell cycle Transition and termination of DNA replication and Cell cycle Chromosome condensation in prometaphase. GO annotations related to this gene include enzyme binding and protein heterodimerization activity. An important paralog of this gene is TOP2A.

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880
Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.
Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation
via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR),
which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene

summary for TOP2B Gene:
Topoisomerases are ubiquitously expressed enzymes that overcome topological problems in genomic DNA, which
can result from DNA replication, transcription and repair. Common problems such as overwinding (positive
supercoiling), knots and tangles are resolved by topoisomerases by catalysing the breaking of one or two
strands of DNA. Topoisomerase type I enzymes cut just one strand of the DNA double helix, and hence relax
supercoiling of the DNA. Four genes encode Type I topoisomerases, which include nuclear topoisomerase I
(TOP1) and mitochondrial topoisomerase I (TOP1MT). Type I enzymes can also be sub-divided into Types A and B
based on their different catalytic mechanism for generating single DNA strand breaks. TOP1 and TOP1MT are
both type 1B since they form 3'-phosphotyrosyl bonds. Camptothecin is a natural product inhibitor of TOP1
that has led to the discovery of derivatives that are clinically used anti-cancer drugs. Such inhibitors
trap the enzyme in a cleavage complex with DNA. Type II topoisomerases (IIA and IIB) are particular
important for the disentanglement of DNA, which is overcome by generating double stranded DNA breaks.
Dysregulation of type II enzymes can therefore have a potentially significant genotoxic effect. There is a
broad range of topoisomerase II inhibitors that are used clinically as anticancer agents.

Gene Wiki entry for TOP2B Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NT_022517.18  NC_018914.2  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for TOP2B
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTOP2B promoter sequence
   Search SABiosciences Chromatin IP Primers for TOP2B

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TOP2B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p24   Ensembl cytogenetic band:  3p24.2   HGNC cytogenetic band: 3p24.2

TOP2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TOP2B gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M025639:  view genomic region     (about GC identifiers)

Start:
25,639,475 bp from pter      End:
25,706,398 bp from pter
Size:
66,924 bases      Orientation:
minus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880 (See protein sequence)
Recommended Name: DNA topoisomerase 2-beta  
Size: 1626 amino acids; 183267 Da
Cofactor: Magnesium. Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and
the DNA. Can also accept other divalent metal cations, such as Mn(2+) and Ca(2+)
Subunit: Homodimer. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1,
SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B
Subcellular location: Cytoplasm. Nucleus, nucleolus
Miscellaneous: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas
prokaryotic enzymes relax only negative supercoils
1 PDB 3D structure from and Proteopedia for TOP2B:
3QX3 (3D)    
Secondary accessions: Q13600 Q9UMG8 Q9UQP8
Alternative splicing: 2 isoforms:  Q02880-1   Q02880-2   

Explore the universe of human proteins at neXtProt for TOP2B: NX_Q02880

Explore proteomics data for TOP2B at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q02880

  • 4/68 DME Specific Peptides for TOP2B (Q02880) (see all 68)
     FDSQTKE  DDRKEWL  NCLIENP  TSSNYDD 

    TOP2B Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    TOP2B Protein Expression
    REFSEQ proteins: NP_001059.2  
    ENSEMBL proteins: 
     ENSP00000388216   ENSP00000396704   ENSP00000391112   ENSP00000264331   ENSP00000446023  
     ENSP00000437352  

    Human Recombinant Protein Products for TOP2B: 
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    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec 
    OriGene Custom Protein Services for TOP2B
    GenScript Custom Purified and Recombinant Proteins Services for TOP2B
    Novus Biologicals TOP2B Protein
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp. 

    Gene Ontology (GO): 5/9 cellular component terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000792colocalizes with heterochromatin IDA9049244
    GO:0000795synaptonemal complex IBA--
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm IDA9049244
    GO:0005694chromosome ----

    TOP2B for ontologies           About GeneDecksing



    TOP2B Antibody Products: 
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    OriGene Custom Antibody Services for TOP2B
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    Novus Biologicals TOP2B Antibodies
    Abcam antibodies for TOP2B
    Browse Antibodies at Cloud-Clone Corp. 
    ThermoFisher Antibodies for TOP2B
    LSBio Antibodies in human, mouse, rat for TOP2B 

    Assay Products for TOP2B: 
    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for TOP2B
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp. 
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    5/14 InterPro protein domains (see all 14):
     IPR002205 Topo_IIA_A/C
     IPR018522 TopoIIA_CS
     IPR013758 Topo_IIA_A/C_ab
     IPR012542 DTHCT
     IPR003594 HATPase_ATP-bd

    Graphical View of Domain Structure for InterPro Entry Q02880

    ProtoNet protein and cluster: Q02880

    5/6 Blocks protein domains (see all 6):
    IPB001154 Topoisomerase II signature
    IPB002205 DNA gyrase/topoisomerase IV
    IPB003594 ATP-binding region
    IPB003957 CCAAT-binding transcription factor subunit A signature
    IPB011558 DNA topoisomerase


    UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880
    Similarity: Belongs to the type II topoisomerase family
    Similarity: Contains 1 Toprim domain


    TOP2B for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: TOP2B_HUMAN, Q02880
    Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.
    Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation
    via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR),
    which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene
    Catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA

         Genatlas biochemistry entry for TOP2B:
    DNA topoisomerase II beta (180kDa),ubiquitous,ATPase component of the topoisomerase II,involved in the breakage
    and rejoining of double strand of DNA,relaxing both positively and negatively supercoiled DNA,target of several
    drugs,widely used in the treatment of human cancers

         Enzyme Number (IUBMB): EC 5.99.1.31 2

         Gene Ontology (GO): 5/11 molecular function terms (GO ID links to tree view) (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IEA--
    GO:0003682chromatin binding IDA9049244
    GO:0003918DNA topoisomerase type II (ATP-hydrolyzing) activity IDA10684600
    GO:0005080protein kinase C binding IPI16611985
    GO:0005515protein binding ----
         
    TOP2B for ontologies           About GeneDecksing


    Phenotypes:
         9 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Top2b):
     behavior/neurological  cellular  embryogenesis  growth/size  mortality/aging 
     nervous system  normal  respiratory system  skeleton 

    TOP2B for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for TOP2B: Top2btm1Jcw Top2btm2.1Jcw

       inGenious Targeting Laboratory - Custom generated mouse model solutions for TOP2B 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for TOP2B

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for TOP2B 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for TOP2B 

    miRNA
    Products:
        
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TOP2B
    8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate TOP2B (see all 14):
    hsa-miR-579 hsa-miR-607 hsa-miR-548d-3p hsa-miR-1283 hsa-miR-409-3p hsa-miR-513a-3p hsa-miR-548e hsa-miR-20b*
    SwitchGear 3'UTR luciferase reporter plasmidTOP2B 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for TOP2B
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TOP2B

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for TOP2B
    Sirion Biotech Customized adenovirus for overexpression of TOP2B

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for TOP2B (see all 5)
    OriGene ORF clones in mouse, rat for TOP2B
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: TOP2B (NM_001068)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP2B
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for TOP2B
    Search LifeMap BioReagents cell lines for TOP2B
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP2B


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for TOP2B About   (see all 6)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Cell cycle Transition and termination of DNA replacation
    Cell cycle Transition and termination of DNA replication0.95
    Cell cycle Transition and termination of DNA replacation0.95
    2Cell cycle Chromosome condensation in prometaphase
    Cell cycle Chromosome condensation in prometaphase1.00
    3DNA damage Role of SUMO in p53 regulation
    Proteolysis Putative SUMO-1 pathway0.33
    4Meiosis
    Meiosis
    5Etoposide Pathway, Pharmacokinetics/Pharmacodynamics
    Etoposide Pathway, Pharmacokinetics/Pharmacodynamics

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    4 EMD Millipore Pathways for TOP2B
        Cell cycle Transition and termination of DNA replacation
    Meiosis
    Proteolysis Putative SUMO-1 pathway
    Cell cycle Chromosome condensation in prometaphase


    3 GeneGo (Thomson Reuters) Pathways for TOP2B
        Cell cycle Chromosome condensation in prometaphase
    Proteolysis Putative SUMO-1 pathway
    Cell cycle Transition and termination of DNA replication

    2 PharmGKB Pathways for TOP2B
        Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics
    Etoposide Pathway, Pharmacokinetics/Pharmacodynamics


    TOP2B for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for TOP2B

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/134 Interacting proteins for TOP2B (Q028803 ENSP000003967044) via UniProtKB, MINT, STRING, and/or I2D (see all 134)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 5/9 biological process terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000712resolution of meiotic recombination intermediates IBA--
    GO:0000819sister chromatid segregation IBA--
    GO:0001764neuron migration IEA--
    GO:0006261DNA-dependent DNA replication IBA--
    GO:0006265DNA topological change IDA10684600

    TOP2B for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    TOP2B for compounds           About GeneDecksing

    EMD Millipore small molecules for TOP2B:
    Small Molecule - inhibitor
    Browse drugs & compounds from Enzo Life Sciences

    Compounds for TOP2B available from Tocris Bioscience    About this table
    CompoundAction CAS #
    CPT 11DNA topoisomerase I inhibitor; antitumor[100286-90-6]
    Doxorubicin hydrochlorideAntitumor antibiotic agent. Inhibits DNA topoisomerase II[25316-40-9]
    EtoposideTopoisomerase II inhibitor[33419-42-0]
    SN 38DNA topoisomerase I inhibitor; antitumor[86639-52-3]
    CamptothecinDNA topoisomerase inhibitor[7689-03-4]

    5 DrugBank Compounds for TOP2B    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    DexrazoxaneDesrazoxane (see all 7)24584-09-6target--17139284 17016423 10592235
    Phosphoaminophosphonic Acid-Adenylate Ester-- --target--17139284 17016423 10592235
    Daunorubicin-- 20830-81-3targetinhibitor1963303 6380596
    3'-THIO-THYMIDINE-5'-PHOSPHATE-- --target--10592235
    EtoposideEPEG (see all 4)33419-42-0targetinhibitor17578914

    10/11 Novoseek inferred chemical compound relationships for TOP2B gene (see all 11)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    amsacrine 80.7 7 8194167 (1), 11774253 (1), 8269948 (1), 7980648 (1) (see all 5)
    mitoxantrone 71.6 2 7980648 (1), 1655025 (1)
    etoposide 63.1 6 7805045 (2), 16914690 (1), 19230382 (1), 8269948 (1)
    gal 1 52.9 1 9025779 (1)
    doxorubicin 48.3 3 17523305 (1), 16957942 (1), 12589037 (1)
    vincristine 27.1 1 11004693 (1)
    cisplatin 23.4 1 20219416 (1)
    adriamycin 17.6 4 7805045 (3)
    genistein 16.4 1 7632161 (1)
    epinephrine 10.7 1 12589037 (1)

    Search CenterWatch for drugs/clinical trials and news about TOP2B

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TOP2B gene: 
    NM_001068.3  

    Unigene Cluster for TOP2B:

    Topoisomerase (DNA) II beta 180kDa
    Hs.475733  [show with all ESTs]
    Unigene Representative Sequence: NM_001068
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000413971 ENST00000435706(uc011awm.1 uc003cdj.2 uc011awn.1 uc021wug.1)
    ENST00000475717 ENST00000424225 ENST00000470132 ENST00000491510 ENST00000264331
    ENST00000542520 ENST00000540199
    Congresses - knowledge worth sharing:  
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
    Products:
         
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TOP2B
    8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate TOP2B (see all 14):
    hsa-miR-579 hsa-miR-607 hsa-miR-548d-3p hsa-miR-1283 hsa-miR-409-3p hsa-miR-513a-3p hsa-miR-548e hsa-miR-20b*
    SwitchGear 3'UTR luciferase reporter plasmidTOP2B 3' UTR sequence
    Inhib. RNA
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    DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP2B
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for TOP2B
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TOP2B
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TOP2B

    Additional mRNA sequence: 

    AB208879.1 AK296510.1 AK297171.1 AK307450.1 AK316492.1 BC021863.1 BC054482.1 M27504.1 
    U54831.1 X53662.1 X68060.1 Z15111.1 Z15115.1 

    24/26 DOTS entries (see all 26):

    DT.95356606  DT.218512  DT.95356610  DT.95356602  DT.100036996  DT.102833608  DT.97767947  DT.95356591 
    DT.100818472  DT.97854739  DT.120925291  DT.95356601  DT.100818466  DT.414721  DT.120925234  DT.100748508 
    DT.120925304  DT.40115718  DT.92326151  DT.92452485  DT.92452497  DT.95082622  DT.99963850  DT.92450337 

    24/466 AceView cDNA sequences (see all 466):

    BU680806 BM820355 BM128666 BQ215095 AU132712 X53662 BU506947 BC021863 
    AI874120 BQ049307 BG260499 AL601608 CA436110 AU119793 BM541838 AA620947 
    U54831 BU069087 BI089460 BU521397 BU179049 AW515712 AA318144 BE503615 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TOP2B expression in normal human tissues (normalized intensities)      TOP2B embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GTTACAAACT
    TOP2B Expression
    About this image


    TOP2B expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/36 selected tissues (see all 36) fully expand
     
     Epithelium
             vagina ; squamous epithelial cells   
     
     Uterus
             uterus, post-menopause ; glandular cells   
     
     Testis (Reproductive System)    fully expand to see all 4 entries
             Leydig Cells Testis Interstitium
             seminal vesicle ; glandular cells   
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Colon (Gastrointestinal Tract)    fully expand to see all 3 entries
             colon ; peripheral nerve/ganglion   

    See TOP2B Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TOP2B

    SOURCE GeneReport for Unigene cluster: Hs.475733
        SABiosciences Expression via Pathway-Focused PCR Arrays including TOP2B: 
              Cancer Drug Targets in human mouse rat
              Cancer Drug Resistance in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for TOP2B gene from 9/15 species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Top2b1 , 5 topoisomerase (DNA) II beta1, 5 91.95(n)1
    96.4(a)1
      14 (7.08 cM)5
    219741  NM_009409.21  NP_033435.21 
     163651795 
    chicken
    (Gallus gallus)
    Aves TOP2B1 topoisomerase (DNA) II beta 180kDa 83.54(n)
    89.53(a)
      395966  NM_205082.1  NP_990413.1 
    lizard
    (Anolis carolinensis)
    Reptilia TOP2B6
    DNA topoisomerase 2
    87(a)
    1 ↔ 1
    GL343380.1(735894-789560)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.199922 Transcribed sequence with moderate similarity to protein more 80.39(n)    BX753336.1 
    zebrafish
    (Danio rerio)
    Actinopterygii top2b1 topoisomerase (DNA) II beta 70.46(n)
    75.51(a)
      569877  NM_001045191.2  NP_001038656.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Top23 meiosis DNA binding 61(a)   37E1   --
    worm
    (Caenorhabditis elegans)
    Secernentea F32A11.43 DNA topoisomerase II (N-terminal
    region)
    59(a)
    (best of 6)
      II(13134927-13137287)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes TOP2(YNL088W)4 Topoisomerase II, relieves torsional strain in DNA more   --   14(457704-461990) 855636  NP_014311.1 
    rice
    (Oryza sativa)
    Liliopsida Os.342022 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 73.45(n)    AK072471.1 


    ENSEMBL Gene Tree for TOP2B (if available)
    TreeFam Gene Tree for TOP2B (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TOP2B gene
    TOP2A2  
    2 SIMAP similar genes for TOP2B using alignment to 6 protein entries:     TOP2B_HUMAN (see all proteins):
    topIIb    TOP2A

    TOP2B for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1363 SNPs in TOP2B are shown (see all 1363)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs626386701,2
    C,F--25639964(+) TCTTCG/AGTTTC 2 /P /L mis12Minor allele frequency- A:0.00NS EU 597
    rs113728511,2
    C--25639973(+) CTAGAT/-TTTTT 1 -- int11Minor allele frequency- -:0.00CSA 2
    rs1497615081,2
    C--25640053(+) GCTTAC/TGTTTA 1 -- int10--------
    rs765496061,2
    C,F--25640070(+) ATTACA/CAATAA 1 -- int13Minor allele frequency- C:0.15CSA WA 122
    rs743291891,2
    C,F--25640105(+) AATGCT/CAACCT 1 -- int11Minor allele frequency- C:0.03EA 120
    rs1457179031,2
    --25640142(+) TTGGAA/GGAAAA 1 -- int10--------
    rs1397429501,2
    C--25640239(+) TATATA/GTTACC 1 -- int10--------
    rs1880776871,2
    --25640343(+) GCACCA/GTGTGT 1 -- int10--------
    rs731533071,2
    C--25640358(+) TGTGTG/ACACTG 1 -- int11Minor allele frequency- A:0.50WA 2
    rs1444826011,2
    --25640393(+) ATCACC/TGAGGT 1 -- int10--------

    HapMap Linkage Disequilibrium report for TOP2B (25639475 - 25706398 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for TOP2B:    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2725054CNV Deletion23290073
    esv271475CNV Insertion20981092
    nsv507089CNV Insertion20534489

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 126431    OMIM disorders: --

    20/27 diseases for TOP2B (see all 27):    About MalaCards
    ataxia telangiectasia    rubella    williams syndrome    ataxia
    medulloblastoma    choroiditis    non-hodgkin lymphoma    hodgkin's lymphoma
    acute lymphoblastic leukemia    astrocytoma    lymphoblastic leukemia    pneumonia
    neuroblastoma    myeloma    leukemia    tuberculosis
    myeloid leukemia    gastric cancer    squamous cell carcinoma    hepatocellular carcinoma


    TOP2B for disorders           About GeneDecksing

    6 Novoseek inferred disease relationships for TOP2B gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    colon carcinoma 38.5 1 7980648 (1)
    leukemia 28.8 2 7980648 (1), 1655025 (1)
    cancer 4.06 1 7797575 (1)
    poisoning 0 1 12769776 (1)
    cancer lung 0 1 8398710 (1)
    tumors 0 5 8547322 (4), 8759604 (1)

    Genetic Association Database (GAD): TOP2B
    Human Genome Epidemiology (HuGE) Navigator: TOP2B (6 documents)

    Export disorders for TOP2B gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TOP2B gene, integrated from 9 sources (see all 176):
    (articles sorted by number of sources associating them with TOP2B)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24. (PubMed id 1333583)1, 2, 3, 9 Jenkins J.R.... Hickson I.D. (1992)
    2. Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication. (PubMed id 10095062)1, 2, 9 Sng J.H.... Fisher L.M. (1999)
    3. Mutagenesis of E477 or K505 in the B' domain of human topoisomerase II beta increases the requirement for magnesium ions during strand passage. (PubMed id 10684600)1, 2, 9 West K.L....Austin C.A. (2000)
    4. Human Variation in Alcohol Response Is Influenced by Variation in Neuronal Signaling Genes. (PubMed id 20201926)1, 4 Joslyn G....White R.L. (2010)
    5. Investigation of genetic susceptibility factors for h uman longevity - A targeted nonsynonymous SNP study. (PubMed id 20800603)1, 4 Flachsbart F....Nebel A. (2010)
    6. Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (2010)
    7. Rubella vaccine-induced cellular immunity: evidence o f associations with polymorphisms in the Toll-like, vitamin A and D receptors, and innate immune response genes. (PubMed id 19902255)1, 4 Ovsyannikova I.G....Poland G.A. (2010)
    8. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (2006)
    9. Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta. (PubMed id 12821127)1, 2 Mirski S.E.... Cole S.P. (2003)
    10. Human cells express two differentially spliced forms of topoisomerase II beta mRNA. (PubMed id 8396237)1, 2 Davies S.L.... Hickson I.D. (1993)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 7155 HGNC: 11990 AceView: TOP2B Ensembl:ENSG00000077097 euGenes: HUgn7155
    ECgene: TOP2B H-InvDB: TOP2B

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TOP2B Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for TOP2B Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TOP2B gene:
    Search GeneIP for patents involving TOP2B

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
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    About This Section

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    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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