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TOP2B Gene

protein-coding   GIFtS: 65
GCID: GC03M025639

Topoisomerase (DNA) II Beta 180kDa

(Previous name: topoisomerase (DNA) II beta (180kD))
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Topoisomerase (DNA) II Beta 180kDa1 2     DNA Topoisomerase II Beta2
DNA Topoisomerase II, Beta Isozyme2 3     DNA Topoisomerase II, 180 KD2
EC 5.99.1.33 8     top2beta2
Topoisomerase (DNA) II Beta (180kD)1     Topo II Beta2
TOPIIB2     Topoisomerase II Beta2
Antigen MLAA-442     Topoisomerase IIb2
DNA Topoisomerase 2-Beta2     U937 Associated Antigen2

External Ids:    HGNC: 119901   Entrez Gene: 71552   Ensembl: ENSG000000770977   OMIM: 1264315   UniProtKB: Q028803   

Export aliases for TOP2B gene to outside databases

Previous GC identifers: GC03M025346 GC03M025489 GC03M025614 GC03M025584


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for TOP2B Gene:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during
transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid
separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes
the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one
another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene
duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is
localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents
and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced
activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results
in two transcript variants; however, the second variant has not yet been fully described. (provided by RefSeq,
Jul 2008)

GeneCards Summary for TOP2B Gene:
TOP2B (topoisomerase (DNA) II beta 180kDa) is a protein-coding gene. GO annotations related to this gene include enzyme binding and protein heterodimerization activity. An important paralog of this gene is TOP2A.

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880
Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.
Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation
via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR),
which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene

summary for TOP2B Gene:
Topoisomerases are ubiquitously expressed enzymes that overcome topological problems in genomic DNA, which
can result from DNA replication, transcription and repair. Common problems such as overwinding (positive
supercoiling), knots and tangles are resolved by topoisomerases by catalysing the breaking of one or two
strands of DNA. Topoisomerase type I enzymes cut just one strand of the DNA double helix, and hence relax
supercoiling of the DNA. Four genes encode Type I topoisomerases, which include nuclear topoisomerase I
(TOP1) and mitochondrial topoisomerase I (TOP1MT). Type I enzymes can also be sub-divided into Types A and B
based on their different catalytic mechanism for generating single DNA strand breaks. TOP1 and TOP1MT are
both type 1B since they form 3'-phosphotyrosyl bonds. Camptothecin is a natural product inhibitor of TOP1
that has led to the discovery of derivatives that are clinically used anti-cancer drugs. Such inhibitors
trap the enzyme in a cleavage complex with DNA. Type II topoisomerases (IIA and IIB) are particular
important for the disentanglement of DNA, which is overcome by generating double stranded DNA breaks.
Dysregulation of type II enzymes can therefore have a potentially significant genotoxic effect. There is a
broad range of topoisomerase II inhibitors that are used clinically as anticancer agents.

Gene Wiki entry for TOP2B Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000003.11  NT_022517.19  NC_018914.2  
Regulatory elements:
   Search for regulatory transcription factor binding sites for TOP2B
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTOP2B promoter sequence
   Search Chromatin IP Primers for TOP2B

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TOP2B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p24   Ensembl cytogenetic band:  3p24.2   HGNC cytogenetic band: 3p24.2

TOP2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TOP2B gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03M025639:  view genomic region     (about GC identifiers)

Start:
25,639,475 bp from pter      End:
25,706,398 bp from pter
Size:
66,924 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880 (See protein sequence)
Recommended Name: DNA topoisomerase 2-beta  
Size: 1626 amino acids; 183267 Da
Cofactor: Magnesium. Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and
the DNA. Can also accept other divalent metal cations, such as Mn(2+) and Ca(2+)
Subunit: Homodimer. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1,
SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B
Miscellaneous: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas
prokaryotic enzymes relax only negative supercoils
5 PDB 3D structures from and Proteopedia for TOP2B:
3QX3 (3D)        4G0U (3D)        4G0V (3D)        4G0W (3D)        4J3N (3D)    
Secondary accessions: Q13600 Q9UMG8 Q9UQP8
Alternative splicing: 2 isoforms:  Q02880-1   Q02880-2   

Explore the universe of human proteins at neXtProt for TOP2B: NX_Q02880

Explore proteomics data for TOP2B at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys117, Lys144, Lys212, Lys254, Lys342, Lys407, Lys412, Lys510, Lys520, Lys541,
                                 Lys550, Lys556, Lys605, Lys635, Lys643, Lys683, Lys712, Lys744, Lys988, Lys1008
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for TOP2B (Q02880) (see all 68)
     FDSQTKE  DDRKEWL  NCLIENP  TSSNYDD 


    See TOP2B Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_001059.2  
    ENSEMBL proteins: 
     ENSP00000388216   ENSP00000396704   ENSP00000391112   ENSP00000264331   ENSP00000446023  
     ENSP00000437352  

    TOP2B Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for TOP2B
    GenScript Custom Purified and Recombinant Proteins Services for TOP2B
    Novus Biologicals TOP2B Protein
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

     
    Search eBioscience for Proteins for TOP2B 

     
    antibodies-online proteins for TOP2B (3 products) 

     
    antibodies-online peptides for TOP2B

    TOP2B Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    R&D Systems Antibodies for TOP2B
    OriGene Antibodies for TOP2B
    OriGene Custom Antibody Services for TOP2B
    Novus Biologicals TOP2B Antibodies
    Abcam antibodies for TOP2B
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibodies for TOP2B
    antibodies-online antibodies for TOP2B (54 products) 

    TOP2B Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for TOP2B
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for TOP2B
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.
    Search eBioscience for ELISAs for TOP2B 
    antibodies-online kits for TOP2B (8 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 15):
     IPR002205 Topo_IIA_A/C
     IPR018522 TopoIIA_CS
     IPR013758 Topo_IIA_A/C_ab
     IPR012542 DTHCT
     IPR003594 HATPase_ATP-bd

    Graphical View of Domain Structure for InterPro Entry Q02880

    ProtoNet protein and cluster: Q02880

    Selected Blocks protein domains (see all 6):
    IPB001154 Topoisomerase II signature
    IPB002205 DNA gyrase/topoisomerase IV
    IPB003594 ATP-binding region
    IPB003957 CCAAT-binding transcription factor subunit A signature
    IPB011558 DNA topoisomerase


    UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880
    Similarity: Belongs to the type II topoisomerase family
    Similarity: Contains 1 Toprim domain


    Find genes that share domains with TOP2B           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: TOP2B_HUMAN, Q02880
    Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.
    Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation
    via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR),
    which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene
    Catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA

         Genatlas biochemistry entry for TOP2B:
    DNA topoisomerase II beta (180kDa),ubiquitous,ATPase component of the topoisomerase II,involved in the breakage
    and rejoining of double strand of DNA,relaxing both positively and negatively supercoiled DNA,target of several
    drugs,widely used in the treatment of human cancers

         Enzyme Number (IUBMB): EC 5.99.1.31 2

         Gene Ontology (GO): Selected molecular function terms (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IEA--
    GO:0003682chromatin binding IDA9049244
    GO:0003918DNA topoisomerase type II (ATP-hydrolyzing) activity IDA10684600
    GO:0005080protein kinase C binding IPI16611985
    GO:0005515protein binding ----
         
    Find genes that share ontologies with TOP2B           About GenesLikeMe


    Phenotypes:
         9 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Top2b):
     behavior/neurological  cellular  embryogenesis  growth/size/body  mortality/aging 
     nervous system  normal  respiratory system  skeleton 

    Find genes that share phenotypes with TOP2B           About GenesLikeMe

    Animal Models:
         MGI mouse knock-outs for TOP2B: Top2btm1Jcw Top2btm2.1Jcw

       genOway: Develop your customized and physiologically relevant rodent model for TOP2B

    miRNA
    Products:
        
    miRTarBase miRNAs that target TOP2B:
    hsa-mir-26b-5p (MIRT029687)

    Block miRNA regulation of human, mouse, rat TOP2B using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate TOP2B (see all 14):
    hsa-miR-579 hsa-miR-607 hsa-miR-548d-3p hsa-miR-1283 hsa-miR-409-3p hsa-miR-513a-3p hsa-miR-548e hsa-miR-20b*
    SwitchGear 3'UTR luciferase reporter plasmidTOP2B 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for TOP2B
    Predesigned siRNA for gene silencing in human, mouse, rat TOP2B

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for TOP2B

    Clone
    Products:
         
    OriGene clones in human, mouse for TOP2B (see all 6)
    OriGene ORF clones in mouse, rat for TOP2B
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: TOP2B (NM_001068)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP2B
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for TOP2B
    Browse ESI BIO Cell Lines and PureStem Progenitors for TOP2B 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP2B


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    TOP2B_HUMAN, Q02880: Cytoplasm. Nucleus, nucleolus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol5
    nucleus5

    Gene Ontology (GO): Selected cellular component terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000792colocalizes with heterochromatin IDA9049244
    GO:0000795synaptonemal complex IBA--
    GO:0005634nucleus IDA--
    GO:0005654nucleoplasm IDA9049244
    GO:0005730NOT nucleolus IDA--

    Find genes that share ontologies with TOP2B           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for TOP2B About   (see all 6)  
    See pathways by source

    SuperPathContained pathways About
    1Telomere C-strand (Lagging Strand) Synthesis
    Cell cycle Transition and termination of DNA replication0.43
    2Proteolysis Putative SUMO 1 pathway
    Proteolysis Putative SUMO 1 pathway0.33
    3Meiosis (EMD)
    Meiosis
    4Etoposide Pathway, Pharmacokinetics/Pharmacodynamics
    Etoposide Pathway, Pharmacokinetics/Pharmacodynamics
    5Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics
    Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    3 GeneGo (Thomson Reuters) Pathways for TOP2B
        Cell cycle Chromosome condensation in prometaphase
    Proteolysis Putative SUMO-1 pathway
    Cell cycle Transition and termination of DNA replication

    2 PharmGKB Pathways for TOP2B
        Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics
    Etoposide Pathway, Pharmacokinetics/Pharmacodynamics

        Pathway & Disease-focused RT2 Profiler PCR Arrays including TOP2B: 
              Cancer Drug Targets in human mouse rat
              Cancer Drug Resistance in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for TOP2B

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for TOP2B (Q028803 ENSP000003967044) via UniProtKB, MINT, STRING, and/or I2D (see all 281)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4FP628053, ENSP000003669744I2D: score=1 STRING: ENSP00000366974
    HIST1H4HP628053, ENSP000002893524I2D: score=1 STRING: ENSP00000289352
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000712resolution of meiotic recombination intermediates IBA--
    GO:0000819sister chromatid segregation IBA--
    GO:0001764neuron migration IEA--
    GO:0006200ATP catabolic process IDA10684600
    GO:0006261DNA-dependent DNA replication IBA--

    Find genes that share ontologies with TOP2B           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
    Selected ApexBio Compounds for TOP2B (see all 20)     About this table
    CompoundAction CAS #
    4'-Demethylepipodophyllotoxinkey intermediate compound for the preparation of podophyllotoxin-type anti-cancer drugs[6559-91-7]
    AmonafideDNA intercalator and Topo II (topoisomerase II) inhibitor[69408-81-7]
    AmrubicinTopoisomerase II inhibitor, anthracycline agent[110267-81-7]
    Camptothecinprototypic DNA topoisomerase I inhibitor[7689-3-4]
    Deferasirox Fe3+ chelaterationally-designed oral iron chelator[554435-83-5]
    Dexrazoxane HCl (ICRF-187, ADR-529)cardioprotective agent[149003-01-0]
    DoxorubicinDNA topoisomerase I inhibitor[23214-92-8]
    Doxorubicin (Adriamycin) HClAntitumor antibiotic agent, inhibits DNA topoisomerase II[25316-40-9]
    Ellagic acidATP-competitive inhibitor of casein kinase 2 (CK2)[476-66-4]
    Epirubicin HClAntibiotic antitumor agent[56390-09-1]

    Compounds for TOP2B available from Tocris Bioscience    About this table
    CompoundAction CAS #
    CPT 11DNA topoisomerase I inhibitor; antitumor[100286-90-6]
    Doxorubicin hydrochlorideAntitumor antibiotic agent. Inhibits DNA topoisomerase II[25316-40-9]
    EtoposideTopoisomerase II inhibitor[33419-42-0]
    SN 38DNA topoisomerase I inhibitor; antitumor[86639-52-3]
    CamptothecinDNA topoisomerase inhibitor[7689-03-4]

    5 DrugBank Compounds for TOP2B    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    DexrazoxaneDesrazoxane (see all 7)24584-09-6target--17139284 17016423 10592235
    Phosphoaminophosphonic Acid-Adenylate Ester-- --target--17139284 17016423 10592235
    Daunorubicin-- 20830-81-3targetinhibitor1963303 6380596
    3'-THIO-THYMIDINE-5'-PHOSPHATE-- --target--10592235
    EtoposideEPEG (see all 4)33419-42-0targetinhibitor17578914

    Selected Novoseek inferred chemical compound relationships for TOP2B gene (see all 11)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    amsacrine 80.7 7 8194167 (1), 11774253 (1), 8269948 (1), 7980648 (1) (see all 5)
    mitoxantrone 71.6 2 7980648 (1), 1655025 (1)
    etoposide 63.1 6 7805045 (2), 16914690 (1), 19230382 (1), 8269948 (1)
    gal 1 52.9 1 9025779 (1)
    doxorubicin 48.3 3 17523305 (1), 16957942 (1), 12589037 (1)
    vincristine 27.1 1 11004693 (1)
    cisplatin 23.4 1 20219416 (1)
    adriamycin 17.6 4 7805045 (3)
    genistein 16.4 1 7632161 (1)
    epinephrine 10.7 1 12589037 (1)



    Find genes that share compounds with TOP2B           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for TOP2B gene: 
    NM_001068.3  

    Unigene Cluster for TOP2B:

    Topoisomerase (DNA) II beta 180kDa
    Hs.475733  [show with all ESTs]
    Unigene Representative Sequence: NM_001068
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000413971 ENST00000435706(uc011awm.1 uc003cdj.2 uc011awn.1 uc021wug.1)
    ENST00000475717 ENST00000424225 ENST00000470132 ENST00000491510 ENST00000264331
    ENST00000542520 ENST00000540199
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat TOP2B using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate TOP2B (see all 14):
    hsa-miR-579 hsa-miR-607 hsa-miR-548d-3p hsa-miR-1283 hsa-miR-409-3p hsa-miR-513a-3p hsa-miR-548e hsa-miR-20b*
    SwitchGear 3'UTR luciferase reporter plasmidTOP2B 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for TOP2B
    Predesigned siRNA for gene silencing in human, mouse, rat TOP2B
    Clone
    Products:
         
    OriGene clones in human, mouse for TOP2B (see all 6)
    OriGene ORF clones in mouse, rat for TOP2B
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: TOP2B (NM_001068)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP2B
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for TOP2B
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat TOP2B
      QuantiTect SYBR Green Assays in human, mouse, rat TOP2B
      QuantiFast Probe-based Assays in human, mouse, rat TOP2B

    Additional mRNA sequence: 

    AB208879.1 AK296510.1 AK297171.1 AK307450.1 AK316492.1 BC021863.1 BC054482.1 M27504.1 
    U54831.1 X53662.1 X68060.1 Z15111.1 Z15115.1 

    Selected DOTS entries (see all 26):

    DT.95356606  DT.218512  DT.95356610  DT.95356602  DT.100036996  DT.102833608  DT.97767947  DT.95356591 
    DT.100818472  DT.97854739  DT.120925291  DT.95356601  DT.100818466  DT.414721  DT.120925234  DT.100748508 
    DT.120925304  DT.40115718  DT.92326151  DT.92452485  DT.92452497  DT.95082622  DT.99963850  DT.92450337 

    Selected AceView cDNA sequences (see all 466):

    BU175251 Z15111 AI089698 BM974392 BG677738 M27504 BM703938 BC021863 
    AI144514 AV713895 BX494928 CR602047 CB155320 BM541838 N63584 BQ430963 
    U54831 CB146250 BQ881088 BM981692 CA773806 AA424448 AA247789 AA620947 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    TOP2B expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: GTTACAAACT
    TOP2B Expression
    About this image


    TOP2B expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 14) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Limb (Muscoskeletal System)
             Limb Bud
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
    TOP2B Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    TOP2B Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.475733
        Pathway & Disease-focused RT2 Profiler PCR Arrays including TOP2B: 
              Cancer Drug Targets in human mouse rat
              Cancer Drug Resistance in human mouse rat

    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for TOP2B
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat TOP2B
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    In Situ
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP2B

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for TOP2B gene from Selected species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Top2b1 , 5 topoisomerase (DNA) II beta1, 5 92.02(n)1
    96.38(a)1
      14 (7.08 cM)5
    219741  NM_009409.21  NP_033435.21 
     163651795 
    chicken
    (Gallus gallus)
    Aves TOP2B1 topoisomerase (DNA) II beta 180kDa 83.44(n)
    89.5(a)
      395966  NM_205082.1  NP_990413.1 
    lizard
    (Anolis carolinensis)
    Reptilia TOP2B6
    topoisomerase (DNA) II beta 180kDa
    87(a)
    1 ↔ 1
    GL343380.1(735894-789560)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.199922 Transcribed sequence with moderate similarity to protein more 80.39(n)    BX753336.1 
    zebrafish
    (Danio rerio)
    Actinopterygii top2b1 topoisomerase (DNA) II beta 70.3(n)
    75.49(a)
      569877  NM_001045191.2  NP_001038656.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Top23 meiosis DNA binding 61(a)   37E1   --
    worm
    (Caenorhabditis elegans)
    Secernentea F32A11.43 DNA topoisomerase II (N-terminal
    region)
    59(a)
    (best of 6)
      II(13134927-13137287)   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes TOP2(YNL088W)4
    TOP21
    Topoisomerase II, relieves torsional strain in DNA more4
    TOP21
    55.85(n)1
    50.67(a)1
      14(457704-461990)4
    8556361, 4  NP_014311.31  NP_014311.14 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons TOPII1 TOPII 58.07(n)
    55.16(a)
      821972  NM_001125214.1  NP_001118686.1 
    rice
    (Oryza sativa)
    Liliopsida Os.342022 Oryza sativa (japonica cultivar-group) cDNA cloneJ more 73.45(n)    AK072471.1 


    ENSEMBL Gene Tree for TOP2B (if available)
    TreeFam Gene Tree for TOP2B (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for TOP2B gene
    TOP2A2  
    2 SIMAP similar genes for TOP2B using alignment to 6 protein entries:     TOP2B_HUMAN (see all proteins):
    topIIb    TOP2A

    Find genes that share paralogs with TOP2B           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for TOP2B (see all 1363)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs626386701,2
    C,F--25639964(+) TCTTCG/AGTTTC 2 /P /L mis12Minor allele frequency- A:0.00NS EU 597
    rs113728511,2
    C--25639973(+) CTAGAT/-TTTTT 1 -- int11Minor allele frequency- -:0.00CSA 2
    rs1497615081,2
    C--25640053(+) GCTTAC/TGTTTA 1 -- int10--------
    rs765496061,2
    C,F--25640070(+) ATTACA/CAATAA 1 -- int13Minor allele frequency- C:0.15CSA WA 122
    rs743291891,2
    C,F--25640105(+) AATGCT/CAACCT 1 -- int11Minor allele frequency- C:0.03EA 120
    rs1457179031,2
    --25640142(+) TTGGAA/GGAAAA 1 -- int10--------
    rs1397429501,2
    C--25640239(+) TATATA/GTTACC 1 -- int10--------
    rs1880776871,2
    --25640343(+) GCACCA/GTGTGT 1 -- int10--------
    rs731533071,2
    C--25640358(+) TGTGTG/ACACTG 1 -- int11Minor allele frequency- A:0.50WA 2
    rs1444826011,2
    --25640393(+) ATCACC/TGAGGT 1 -- int10--------

    HapMap Linkage Disequilibrium report for TOP2B (25639475 - 25706398 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for TOP2B:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2725054CNV Deletion23290073
    esv271475CNV Insertion20981092
    nsv507089CNV Insertion20534489

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing TOP2B
    DNA2.0 Custom Variant and Variant Library Synthesis for TOP2B

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 126431    OMIM disorders: --


    Find genes that share disorders with TOP2B           About GenesLikeMe

    6 Novoseek inferred disease relationships for TOP2B gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    colon carcinoma 38.5 1 7980648 (1)
    leukemia 28.8 2 7980648 (1), 1655025 (1)
    cancer 4.06 1 7797575 (1)
    poisoning 0 1 12769776 (1)
    cancer lung 0 1 8398710 (1)
    tumors 0 5 8547322 (4), 8759604 (1)

    Genetic Association Database (GAD): TOP2B
    Human Genome Epidemiology (HuGE) Navigator: TOP2B (6 documents)

    Export disorders for TOP2B gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for TOP2B gene, integrated from 10 sources (see all 178):
    (articles sorted by number of sources associating them with TOP2B)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24. (PubMed id 1333583)1, 2, 3, 9 Jenkins J.R.... Hickson I.D. (Nucleic Acids Res. 1992)
    2. Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication. (PubMed id 10095062)1, 2, 9 Sng J.H.... Fisher L.M. (Biochim. Biophys. Acta 1999)
    3. Mutagenesis of E477 or K505 in the B' domain of human topoisomerase II beta increases the requirement for magnesium ions during strand passage. (PubMed id 10684600)1, 2, 9 West K.L....Austin C.A. (Biochemistry 2000)
    4. Human variation in alcohol response is influenced by variation in neuronal signaling genes. (PubMed id 20201926)1, 4 Joslyn G....White R.L. (Alcohol. Clin. Exp. Res. 2010)
    5. Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study. (PubMed id 20800603)1, 4 Flachsbart F....Nebel A. (Mutat. Res. 2010)
    6. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (Diabetes Care 2010)
    7. Rubella vaccine-induced cellular immunity: evidence of associations with polymorphisms in the Toll-like, vitamin A and D receptors, and innate immune response genes. (PubMed id 19902255)1, 4 Ovsyannikova I.G....Poland G.A. (Hum. Genet. 2010)
    8. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (Cell 2006)
    9. Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta. (PubMed id 12821127)1, 2 Mirski S.E.... Cole S.P. (Biochem. Biophys. Res. Commun. 2003)
    10. Human cells express two differentially spliced forms of topoisomerase II beta mRNA. (PubMed id 8396237)1, 2 Davies S.L.... Hickson I.D. (Nucleic Acids Res. 1993)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 7155 HGNC: 11990 AceView: TOP2B Ensembl:ENSG00000077097 euGenes: HUgn7155
    ECgene: TOP2B H-InvDB: TOP2B

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for TOP2B Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for TOP2B Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

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    Patent Information for TOP2B gene:
    Search GeneIP for patents involving TOP2B

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from genOway)
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