Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

TOP2B Gene

protein-coding GIFtS: 63
GCID: GC03M025639

topoisomerase (DNA) II beta 180kDa

(Previous name: topoisomerase (DNA) II beta (180kD) )

Explore 31 diseases affiliated with
TOP2B via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Topoisomerase (DNA) II Beta 180kDa¹ ²		DNA Topoisomerase II Beta²
DNA Topoisomerase II, Beta Isozyme² ³		DNA Topoisomerase II, 180 KD²
EC 5.99.1.3³ ⁸		Top2beta¹
Topoisomerase (DNA) II Beta (180kD)¹		Topo II Beta²
TOPIIB²		Topoisomerase II Beta²
Antigen MLAA-44²		Topoisomerase IIb²
DNA Topoisomerase 2-Beta²		U937 Associated Antigen²

External Ids:	HGNC: 11990¹	Entrez Gene: 7155²	Ensembl: ENSG00000077097⁷	OMIM: 126431⁵	UniProtKB: Q02880³

Export aliases for TOP2B gene to outside databases

Previous GC identifers: GC03M025346 GC03M025489 GC03M025614 GC03M025584

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TOP2B:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during

transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and

the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient

breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus

altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene

encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene

encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene

have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in

ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second

variant has not yet been fully described. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880

Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.

Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation via its

association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is

required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene

summary for TOP2B:

Topoisomerases are ubiquitously expressed enzymes that overcome topological problems in genomic DNA, which

can result from DNA replication, transcription and repair. Common problems such as overwinding (positive

supercoiling), knots and tangles are resolved by topoisomerases by catalysing the breaking of one or two

strands of DNA. Topoisomerase type I enzymes cut just one strand of the DNA double helix, and hence relax

supercoiling of the DNA. Four genes encode Type I topoisomerases, which include nuclear topoisomerase I

(TOP1) and mitochondrial topoisomerase I (TOP1MT). Type I enzymes can also be sub-divided into Types A and B

based on their different catalytic mechanism for generating single DNA strand breaks. TOP1 and TOP1MT are

both type 1B since they form 3'-phosphotyrosyl bonds. Camptothecin is a natural product inhibitor of TOP1

that has led to the discovery of derivatives that are clinically used anti-cancer drugs. Such inhibitors

trap the enzyme in a cleavage complex with DNA. Type II topoisomerases (IIA and IIB) are particular

important for the disentanglement of DNA, which is overcome by generating double stranded DNA breaks.

Dysregulation of type II enzymes can therefore have a potentially significant genotoxic effect. There is a

broad range of topoisomerase II inhibitors that are used clinically as anticancer agents.

Gene Wiki entry for TOP2B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000003.11 NC_018914.1 NT_022517.18

Regulatory elements:

Search SABiosciences Regulatory transcription factor binding sites for TOP2B
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: TOP2B promoter sequence

Search SABiosciences Chromatin IP Primers for TOP2B

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TOP2B

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p24 Ensembl cytogenetic band: 3p24.2 HGNC cytogenetic band: 3p24.2

TOP2B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TOP2B gene location

GeneLoc information about chromosome 3 GeneLoc Exon Structure

GeneLoc location for GC03M025639: view genomic region (about GC identifiers)

Start:	25,639,475 bp from pter	End:	25,706,398 bp from pter
Size:	66,924 bases	Orientation:	minus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880 (See protein sequence)

Recommended Name: DNA topoisomerase 2-beta

Size: 1626 amino acids; 183267 Da

Cofactor: Magnesium. Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the

DNA. Can also accept other divalent metal cations, such as Mn(2+) and Ca(2+)

Subunit: Homodimer. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2,

SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B

Subcellular location: Cytoplasm. Nucleus, nucleolus

Miscellaneous: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic

enzymes relax only negative supercoils

1 PDB 3D structure from and Proteopedia for TOP2B:

3QX3 (3D)

Secondary accessions: Q13600 Q9UMG8 Q9UQP8

Alternative splicing: 2 isoforms: Q02880-1 Q02880-2

Explore the universe of human proteins at neXtProt for TOP2B: NX_Q02880

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q02880

4/68 DME Specific Peptides for TOP2B (Q02880) (see all 68)

FDSQTKE

DDRKEWL

NCLIENP

TSSNYDD

TOP2B Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_001059.2

ENSEMBL proteins:

ENSP00000388216 ENSP00000396704 ENSP00000391112 ENSP00000264331 ENSP00000446023

ENSP00000437352

Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells Browse OriGene Protein Over-expression Lysates OriGene Custom Protein Services for TOP2B
	GenScript Custom Purified and Recombinant Proteins Services for TOP2B
	Novus Biologicals TOP2B Protein
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Browse Proteins at Uscn

Gene Ontology (GO): 5/9 cellular component terms (GO ID links to tree view) (see all 9): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000792	colocalizes with heterochromatin	IDA	9049244
GO:0000795	synaptonemal complex	IBA	--
GO:0005634	nucleus	IDA	--
GO:0005654	nucleoplasm	IDA	9049244
GO:0005694	chromosome	--	--

TOP2B for ontologies About GeneDecksing

TOP2B Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	R&D Systems Antibodies for TOP2B
	OriGene Antibodies: TOP2B OriGene Custom Antibody Services for TOP2B
	GenScript Custom Superior Antibodies Services for TOP2B
	Novus Biologicals TOP2B Antibodies
	Abcam antibodies for TOP2B
	Browse Antibodies at Uscn
	ThermoFisher Antibodies for TOP2B

Assay Products for TOP2B:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for TOP2B
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs and CLIAs at Uscn

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TOP2B for domains About GeneDecksing

5/14 InterPro domains/families (see all 14):

IPR002205 Topo_IIA_A/C

IPR018522 TopoIIA_CS

IPR013758 Topo_IIA_A/C_ab

IPR012542 DTHCT

IPR003594 ATPase-like_ATP-bd

Graphical View of Domain Structure for InterPro Entry Q02880

ProtoNet protein and cluster: Q02880

5/6 Blocks protein families (see all 6):

IPB001154 Topoisomerase II signature

IPB002205 DNA gyrase/topoisomerase IV

IPB003594 ATP-binding region

IPB003957 CCAAT-binding transcription factor subunit A signature

IPB011558 DNA topoisomerase

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880

Similarity: Belongs to the type II topoisomerase family

Similarity: Contains 1 Toprim domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: TOP2B_HUMAN, Q02880

Function: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.

Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D-coupled transcription regulation via its

association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is

required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene

Catalytic activity: ATP-dependent breakage, passage and rejoining of double-stranded DNA

Genatlas biochemistry entry for TOP2B:

DNA topoisomerase II beta (180kDa),ubiquitous,ATPase component of the topoisomerase II,involved in the breakage and

rejoining of double strand of DNA,relaxing both positively and negatively supercoiled DNA,target of several

drugs,widely used in the treatment of human cancers

Enzyme Number (IUBMB): EC 5.99.1.3¹ ²

miRNA Products:		OriGene 3'-UTR Clone: TOP2B Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TOP2B
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate TOP2B (see all 14):
		hsa-miR-579 hsa-miR-607 hsa-miR-548d-3p hsa-miR-1283 hsa-miR-409-3p hsa-miR-513a-3p hsa-miR-548e hsa-miR-20b*

		SwitchGear 3'UTR luciferase reporter plasmid: TOP2B 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TOP2B (see all 4) OriGene shRNA RFP: TOP2B OriGene siRNA: TOP2B
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TOP2B

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for TOP2B

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TOP2B (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TOP2B OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TOP2B (NM_001068)
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP2B
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for TOP2B
		Search LifeMap BioReagents cell lines for TOP2B

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP2B

Gene Ontology (GO): 5/11 molecular function terms (GO ID links to tree view) (see all 11): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003682	chromatin binding	IDA	9049244
GO:0003918	DNA topoisomerase type II (ATP-hydrolyzing) activity	IDA	10684600
GO:0005080	protein kinase C binding	IPI	16611985
GO:0005515	protein binding	--	--
GO:0005524	ATP binding	IEA	--

TOP2B for ontologies           About GeneDecksing

Animal Models:
     Mouse knock-outs for TOP2B: Top2b^tm1Jcw Top2b^tm2.1Jcw
     9 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Top2b):

behavior/neurological	cellular	embryogenesis	growth/size	mortality/aging
nervous system	normal	respiratory system	skeleton

TOP2B for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/6 super-pathways (see all 6) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Cell cycle Chromosome condensation in prometaphase

Cell cycle Chromosome condensation in prometaphase

1.00

Cell cycle_Chromosome condensation in prometaphase

1.00

Proteolysis Putative SUMO-1 pathway

Proteolysis Putative SUMO-1 pathway

1.00

Proteolysis_Putative SUMO-1 pathway

0.97

Cell cycle Transition and termination of DNA replacation

Cell cycle Transition and termination of DNA replacation

1.00

Cell cycle_Transition and termination of DNA replication

0.95

Etoposide Pathway, Pharmacokinetics/Pharmacodynamics

Etoposide Pathway, Pharmacokinetics/Pharmacodynamics

1.00

Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics

Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics

1.00

Pathway sources
See GeneCards unified pathways
Show all pathways

4 EMD Millipore Pathways for TOP2B

	Cell cycle Transition and termination of DNA replacation
	Meiosis
	Proteolysis Putative SUMO-1 pathway
	Cell cycle Chromosome condensation in prometaphase

3 GeneGo (Thomson Reuters) Pathways for TOP2B

	Cell cycle Chromosome condensation in prometaphase
	Proteolysis Putative SUMO-1 pathway
	Cell cycle Transition and termination of DNA replication

2 PharmGKB Pathways for TOP2B

	Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics
	Etoposide Pathway, Pharmacokinetics/Pharmacodynamics

TOP2B for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for TOP2B

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/129 Interacting proteins for TOP2B (Q02880³ ENSP00000396704⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 129)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
HIST1H4A	P62805³	I2D: score=1
HIST1H4B	P62805³	I2D: score=1
HIST1H4C	P62805³	I2D: score=1
HIST1H4D	P62805³	I2D: score=1
HIST1H4E	P62805³	I2D: score=1

About this table

Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000712	resolution of meiotic recombination intermediates	IBA	--
GO:0000819	sister chromatid segregation	IBA	--
GO:0001764	neuron migration	IEA	--
GO:0006261	DNA-dependent DNA replication	IBA	--
GO:0006265	DNA topological change	IDA	10684600

TOP2B for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

TOP2B for compounds About GeneDecksing

EMD Millipore small molecules for TOP2B:
Small Molecule - inhibitor

Browse drugs & compounds from Enzo Life Sciences

Compounds for TOP2B available from Tocris Bioscience About this table

Compound	Action	CAS #
Doxorubicin hydrochloride	Antitumor antibiotic agent. Inhibits DNA topoisomerase II	[25316-40-9]
Epirubicin hydrochloride	Inhibits DNA synthesis and function. Inhibits DNA topoisomerase II.	[56390-09-1]
Etoposide	Topoisomerase II inhibitor	[33419-42-0]
Mitoxantrone dihydrochloride	Topoisomerase II inhibitor; immunosuppressive and antineoplastic agent	[70476-82-3]
Banoxantrone dihydrochloride	Prodrug topoisomerase II inhibitor	[136470-65-0]

4 DrugBank Compounds for TOP2B About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Dexrazoxane	Desrazoxane (see all 7)	24584-09-6	target	--	17139284 17016423 10592235
Phosphoaminophosphonic Acid-Adenylate Ester	--	--	target	--	17139284 17016423 10592235
Daunorubicin	--	20830-81-3	target	inhibitor	1963303 6380596
3'-THIO-THYMIDINE-5'-PHOSPHATE	--	--	target	--	10592235

10/11 Novoseek chemical compound relationships for TOP2B gene (see all 11) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
amsacrine	80.7	7	8194167 (1), 11774253 (1), 8269948 (1), 7980648 (1) (see all 5)
mitoxantrone	71.6	2	7980648 (1), 1655025 (1)
etoposide	63.1	6	7805045 (2), 16914690 (1), 19230382 (1), 8269948 (1)
gal 1	52.9	1	9025779 (1)
doxorubicin	48.3	3	17523305 (1), 16957942 (1), 12589037 (1)
vincristine	27.1	1	11004693 (1)
cisplatin	23.4	1	20219416 (1)
adriamycin	17.6	4	7805045 (3)
genistein	16.4	1	7632161 (1)
epinephrine	10.7	1	12589037 (1)

Search CenterWatch for drugs/clinical trials and news about TOP2B

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for TOP2B gene:

NM_001068.3

Unigene Cluster for TOP2B:

Topoisomerase (DNA) II beta 180kDa

Hs.475733 [show with all ESTs]

Unigene Representative Sequence: NM_001068

9 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000413971 ENST00000435706(uc011awm.1 uc003cdj.2 uc011awn.1 uc021wug.1)

ENST00000475717 ENST00000424225 ENST00000470132 ENST00000491510 ENST00000264331

ENST00000542520 ENST00000540199

miRNA Products:		OriGene 3'-UTR Clone: TOP2B Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TOP2B
		8/14 QIAGEN miScript miRNA Assays for microRNAs that regulate TOP2B (see all 14):
		hsa-miR-579 hsa-miR-607 hsa-miR-548d-3p hsa-miR-1283 hsa-miR-409-3p hsa-miR-513a-3p hsa-miR-548e hsa-miR-20b*

		SwitchGear 3'UTR luciferase reporter plasmid: TOP2B 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TOP2B (see all 4) OriGene shRNA RFP: TOP2B OriGene siRNA: TOP2B
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TOP2B

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TOP2B (see all 2) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TOP2B OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector: TOP2B (NM_001068)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for TOP2B
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TOP2B Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat TOP2B
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TOP2B
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TOP2B

Additional cDNA sequence:

AB208879.1 AK296510.1 AK297171.1 AK307450.1 AK316492.1 BC021863.1 BC054482.1 M27504.1

U54831.1 X53662.1 X68060.1 Z15111.1 Z15115.1

24/25 DOTS entries (see all 25):

DT.95356606 DT.218512 DT.95356610 DT.95356602 DT.100036996 DT.102833608 DT.97767947 DT.95356591

DT.100818472 DT.97854739 DT.120925291 DT.95356601 DT.100818466 DT.120925234 DT.120925304 DT.40115718

DT.414721 DT.92326151 DT.92452485 DT.92452497 DT.95082622 DT.99963850 DT.92450337 DT.92452487

24/466 AceView cDNA sequences (see all 466):

CB112575 BI089460 BU687027 NM_001068 AA424448 BF514624 CR621848 AI874120

T16719 AA953079 BU622293 BQ223532 AL546548 CA442522 BM782486 BU506947

BU674030 BQ215095 D17023 BM801881 T59934 AA247789 CR600074 AI478835

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

TOP2B expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTTACAAACT

About this image

TOP2B expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

3 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Kidney

Cap Mesenchyme

Cap Mesenchyme Cells

Kidney

Kidney

Reproductive System

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See TOP2B Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TOP2B

SOURCE GeneReport for Unigene cluster: Hs.475733

SABiosciences Expression via Pathway-Focused PCR Arrays including TOP2B:

Cancer Drug Targets in human mouse rat

Cancer Drug Resistance in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TOP2B Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat TOP2B
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TOP2B
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TOP2B

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP2B

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the last universal common ancestor (LUCA).

Orthologs for TOP2B gene from 10/31 species (see all 31) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Top2b^{1 , 5}	topoisomerase (DNA) II beta^{1, 5}	91.95(n)¹ 96.4(a)¹		14 (7.08 cM)⁵ 21974¹ NM_009409.2¹ NP_033435.2¹ 16365179⁵
chicken (Gallus gallus)	Aves	TOP2B¹	topoisomerase (DNA) II beta 180kDa	83.54(n) 89.53(a)		395966 NM_205082.1 NP_990413.1
lizard (Anolis carolinensis)	Reptilia	TOP2B⁶	--	87(a)	1 ↔ 1	GL343380.1(735894-789196)
tropical clawed frog (Xenopus tropicalis)	Amphibia	Str.19992²	Transcribed sequence with moderate similarity to protein more	80.39(n)		BX753336.1
zebrafish (Danio rerio)	Actinopterygii	top2b¹	topoisomerase (DNA) II beta	70.46(n) 75.51(a)		569877 NM_001045191.2 NP_001038656.1
fruit fly (Drosophila melanogaster)	Insecta	Top2³	meiosis DNA binding	61(a)		37E1 --
worm (Caenorhabditis elegans)	Secernentea	F32A11.4³	DNA topoisomerase II (N-terminal region)	59(a) (best of 6)		II(13134927-13137287) --
thale cress (Arabidopsis thaliana)	eudicotyledons	TOPII⁶ AT5G04130⁶ (see all 3)	DNA gyrase subunit B (see all 3)	43(a) 20(a) (see all 3)	possible ortholog possible ortholog (see all 3)	3(8624750-8631327) 5(1121828-1128091)
rice (Oryza sativa)	Liliopsida	Os.34202²	Oryza sativa (japonica cultivar-group) cDNA cloneJ more	73.45(n)		AK072471.1
E. coli (Escherichia coli)	Gamma proteobacteria	parE⁶	DNA topoisomerase IV, subunit B	20(a)	possible ortholog	Chromosome(3171526-3173418)

ENSEMBL Gene Tree for TOP2B (if available)
TreeFam Gene Tree for TOP2B (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for TOP2B gene

TOP2A²

2 SIMAP similar genes for TOP2B using alignment to 6 protein entries: TOP2B_HUMAN (see all proteins):

topIIb TOP2A

TOP2B for paralogs About GeneDecksing

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 3 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs62638670^1,2	C,F,	--	25639964(+)	`TCTTCG/AGTTTC`	2	/P /L	mis¹		2		NS EU	597
rs11372851^1,2	C,	--	25639973(+)	`CTAGAT/-TTTTT`	1	--	int¹		1		CSA	2
rs149761508^1,2		--	25640053(+)	`GCTTAC/TGTTTA`	1	--	int¹		0	--	--	--	--
rs76549606^1,2	C,F,	--	25640070(+)	`ATTACA/CAATAA`	1	--	int¹		3		CSA WA	122
rs74329189^1,2	C,F,	--	25640105(+)	`AATGCT/CAACCT`	1	--	int¹		1		EA	120
rs145717903^1,2		--	25640142(+)	`TTGGAA/GGAAAA`	1	--	int¹		0	--	--	--	--
rs139742950^1,2		--	25640239(+)	`TATATA/GTTACC`	1	--	int¹		0	--	--	--	--
rs188077687^1,2		--	25640343(+)	`GCACCA/GTGTGT`	1	--	int¹		0	--	--	--	--
rs73153307^1,2	C,	--	25640358(+)	`TGTGTG/ACACTG`	1	--	int¹		1		WA	2
rs144482601^1,2		--	25640393(+)	`ATCACC/TGAGGT`	1	--	int¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for TOP2B (25639475 - 25706398 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for TOP2B: --

SABiosciences Cancer Mutation PCR Assays

QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TOP2B

DNA2.0 Custom Variant and Variant Library Synthesis for TOP2B

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

TOP2B for disorders           About GeneDecksing

OMIM gene information: 126431    OMIM disorders: --

20/31 diseases for TOP2B (see all 31):    About MalaCards

dna topoisomerase ii ataxia telangiectasia dna topoisomerase i non-hodgkin lymphoma

hodgkin's lymphoma acute lymphoblastic leukemia lymphoblastic leukemia ataxia

williams syndrome squamous cell carcinoma rubella myeloid leukemia

leukemia medulloblastoma hepatocellular carcinoma ovarian carcinoma

carcinoma choroiditis gastric cancer lung cancer

6 Novoseek disease relationships for TOP2B gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
colon carcinoma	38.5	1	7980648 (1)
leukemia	28.8	2	7980648 (1), 1655025 (1)
cancer	4.06	1	7797575 (1)
poisoning	0	1	12769776 (1)
cancer lung	0	1	8398710 (1)
tumors	0	5	8547322 (4), 8759604 (1)

Human Genome Epidemiology (HuGE) Navigator: TOP2B (6 documents)

Export disorders for TOP2B gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for TOP2B gene, integrated from 9 sources (see all 171):
(articles sorted by number of sources associating them with TOP2B)

Utopia: connect your pdf to the dynamic
world of online information

Isolation of cDNA clones encoding the beta isozyme of human DNA topoisomerase II and localisation of the gene to chromosome 3p24. (PubMed id 1333583)^{1, 2, 3, 9} Jenkins J.R.... Hickson I.D. (1992)
Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication. (PubMed id 10095062)^{1, 2, 9} Sng J.H.... Fisher L.M. (1999)
Mutagenesis of E477 or K505 in the B' domain of human topoisomerase II beta increases the requirement for magnesium ions during strand passage. (PubMed id 10684600)^{1, 2, 9} West K.L....Austin C.A. (2000)
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)^{1, 2} Olsen J.V....Mann M. (2006)
Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta. (PubMed id 12821127)^{1, 2} Mirski S.E.... Cole S.P. (2003)
Human cells express two differentially spliced forms of topoisomerase II beta mRNA. (PubMed id 8396237)^{1, 2} Davies S.L.... Hickson I.D. (1993)
Novel HeLa topoisomerase II is the II beta isoform: complete coding sequence and homology with other type II topoisomerases. (PubMed id 8383537)^{1, 2} Austin C.A.... Fisher L.M. (1993)
Topoisomerase II alpha and topoisomerase II beta genes: characterization and mapping to human chromosomes 17 and 3, respectively. (PubMed id 1309226)^{1, 3} Tan K.B....Mao J. (1992)
Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells. (PubMed id 2163884)^{1, 2} Austin C.A. and Fisher L.M. (1990)
Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes. (PubMed id 2556712)^{1, 2} Chung T.D....Mirabelli C.K. (1989)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 7155	HGNC: 11990	AceView: TOP2B	Ensembl:ENSG00000077097	euGenes: HUgn7155
ECgene: TOP2B	H-InvDB: TOP2B

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for TOP2B	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for TOP2B	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for TOP2B gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for TOP2B gene:
Search GeneIP for patents involving TOP2B

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section

	Browse for Gene Knock-down Tools from EMD Millipore
	EMD Millipore small molecules (inhibitor) for TOP2B
	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
	Browse Kits and Assays available from EMD Millipore
	Browse Purified and Recombinant Proteins at EMD Millipore

	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Antibodies for TOP2B (TOP2B)		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Phosphatase Activity Assays/Reagents		Browse Recombinant/Natural Proteins

	OriGene Antibodies for TOP2B		OriGene shRNA RFP for TOP2B
	OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TOP2B		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TOP2B
	Browse OriGene Protein Over-expression Lysates		Browse OriGene Fluorogenic Cell Assay Kits
	OriGene siRNA for TOP2B		OriGene 3'-UTR Clone for TOP2B
	OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TOP2B		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TOP2B
	Browse OriGene GFP tagged cDNA clones in CMV expression vector		Browse OriGene MicroRNA Expression Plasmids
	Browse OriGene basic RS shRNAs		Browse OriGene validated miRNA SYBR primer pairs
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for TOP2B		OriGene Custom Protein Services for TOP2B
	OriGene Custom Immunoassay Development

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat TOP2B
	QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TOP2B
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TOP2B
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat TOP2B
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TOP2B
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TOP2B

	GenScript Custom Purified and Recombinant Proteins Services for TOP2B		GenScript cDNA clones with any tag delivered in your preferred vector for TOP2B
	GenScript Custom Assay Services for TOP2B		GenScript Custom Superior Antibodies Services for TOP2B
	GenScript Custom overexpressing Cell Line Services for TOP2B		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Search Regulatory tfbs for TOP2B
	Search Chromatin IP Primers for TOP2B
	RT² qPCR Primer Assay in human, mouse, rat TOP2B
	GNC Network for TOP2B
	SABiosciences PCR Arrays including human, mouse, rat TOP2B

Tocris compounds for TOP2B

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological cDNA Clones
	Antibodies/Proteins Production Services
	Rabbit Monoclonal Antibody Platform
	Bulk Purchasing

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	TOP2B antibodies
	TOP2B proteins

	Antibodies for TOP2B
	See all of Abcam's Antibodies, Kits and Proteins for TOP2B
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

Browse ELISAs, CLIAs, Proteins, and Antibodies at Uscn

Search LifeMap BioReagents cell lines for TOP2B

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TOP2B

	SwitchGear 3'UTR luciferase reporter plasmids for TOP2B
	SwitchGear Promoter luciferase reporter plasmids for TOP2B

ThermoFisher Antibodies for TOP2B

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TOP2B

GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

View Random Gene

Category			VWF (GIFTS: 73) von Willebrand factor
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes TOP2B gene at Home site.

hostname: 356977-web1.xennexinc.com index build: 100 solr: 1.4

Advanced Search

Export list of displayed genes

TOP2B Gene

topoisomerase (DNA) II beta 180kDa

Aliases
for TOP2B gene

Summaries
for TOP2B gene

Genomic Views
for TOP2B gene

Proteins
for TOP2B gene

Protein Domains /
Families
for TOP2B gene

Function
for TOP2B gene

Pathways & Interactions
for TOP2B gene

Drugs & Compounds
for TOP2B gene

Transcripts
for TOP2B gene

Expression
for TOP2B gene

Orthologs
for TOP2B gene

Paralogs
for TOP2B gene

Genomic Variants
for TOP2B gene

Disorders / Diseases
for TOP2B gene

Publications
for TOP2B gene

External Searches for TOP2B gene

Genome Databases showing TOP2B gene

Other Databases showing TOP2B gene

Specialized Databases showing TOP2B gene

Intellectual Property
for TOP2B gene

Products
for TOP2B gene

View Random Gene

Advanced Search

Export list of displayed genes

TOP2B Gene

topoisomerase (DNA) II beta 180kDa

Aliasesfor TOP2B gene

Summariesfor TOP2B gene

Genomic Viewsfor TOP2B gene

Proteinsfor TOP2B gene

Protein Domains /Familiesfor TOP2B gene

Functionfor TOP2B gene

Pathways & Interactionsfor TOP2B gene

Drugs & Compoundsfor TOP2B gene

Transcriptsfor TOP2B gene

Expression for TOP2B gene

Orthologsfor TOP2B gene

Paralogsfor TOP2B gene

Genomic Variantsfor TOP2B gene

Disorders / Diseasesfor TOP2B gene

Publicationsfor TOP2B gene

External Searches for TOP2B gene

Genome Databases showing TOP2B gene

Other Databases showing TOP2B gene

Specialized Databases showing TOP2B gene

Intellectual Propertyfor TOP2B gene

Productsfor TOP2B gene

View Random Gene

Aliases
for TOP2B gene

Summaries
for TOP2B gene

Genomic Views
for TOP2B gene

Proteins
for TOP2B gene

Protein Domains /
Families
for TOP2B gene

Function
for TOP2B gene

Pathways & Interactions
for TOP2B gene

Drugs & Compounds
for TOP2B gene

Transcripts
for TOP2B gene

Expression
for TOP2B gene

Orthologs
for TOP2B gene

Paralogs
for TOP2B gene

Genomic Variants
for TOP2B gene

Disorders / Diseases
for TOP2B gene

Publications
for TOP2B gene

Intellectual Property
for TOP2B gene

Products
for TOP2B gene