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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TMOD3 Gene

protein-coding   GIFtS: 52
GCID: GC15P052121

tropomodulin 3 (ubiquitous)

 Explore 5 diseases affiliated with
TMOD3 via our new
 Human Malady Compendium 
Biological research products
for TMOD3
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tropomodulin 3 (Ubiquitous)1 2
UTMOD1 2
Ubiquitous Tropomodulin2 3
Tropomodulin-31
U-Tmod3

External Ids:    HGNC: 118731   Entrez Gene: 297662   Ensembl: ENSG000001385947   OMIM: 6051125   UniProtKB: Q9NYL93   

Export aliases for TMOD3 gene to outside databases

Previous GC identifers: GC15P047679 GC15P045167 GC15P049701 GC15P049837 GC15P049909 GC15P028953


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: TMOD3_HUMAN, Q9NYL9
Function: Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex
contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane
skeleton (By similarity)

Gene Wiki entry for TMOD3


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000015.9  NC_018926.1  NT_010194.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TMOD3 gene promoter:
         GR   Egr-3   Pbx1a   GR-beta   Egr-4   Egr-2   SRY   S8   HFH-1   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTMOD3 promoter sequence
   Search SABiosciences Chromatin IP Primers for TMOD3

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TMOD3


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q21.1-q21.2   Ensembl cytogenetic band:  15q21.2   HGNC cytogenetic band: 15q21.1-q21.2

TMOD3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TMOD3 gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15P052121:  view genomic region     (about GC identifiers)

Start:
52,121,825 bp from pter      End:
52,239,492 bp from pter
Size:
117,668 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TMOD3_HUMAN, Q9NYL9 (See protein sequence)
Recommended Name: Tropomodulin-3  
Size: 352 amino acids; 39595 Da
Subunit: Binds to the N-terminus of tropomyosin and to actin (By similarity)
Subcellular location: Cytoplasm, cytoskeleton (By similarity)
Secondary accessions: B2R6G7 Q9NT43 Q9NZR0

Explore the universe of human proteins at neXtProt for TMOD3: NX_Q9NYL9

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9NYL9

  • TMOD3 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_055362.1  
    ENSEMBL proteins: 
     ENSP00000454161   ENSP00000308753   ENSP00000454040   ENSP00000452939   ENSP00000438909  

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    ProSpec Recombinant Protein for TMOD3
    Uscn Proteins for TMOD3

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm ----
    GO:0005856cytoskeleton ----
    GO:0005865striated muscle thin filament IEA--


    TMOD3 for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for TMOD3


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TMOD3 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR004934 Tropomodulin

    Graphical View of Domain Structure for InterPro Entry Q9NYL9

    ProtoNet protein and cluster: Q9NYL9

    1 Blocks protein family: IPB004934 Tropomodulin

    UniProtKB/Swiss-Prot: TMOD3_HUMAN, Q9NYL9
    Similarity: Belongs to the tropomodulin family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: TMOD3_HUMAN, Q9NYL9
    Function: Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex
    contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane
    skeleton (By similarity)

         Genatlas biochemistry entry for TMOD3:
    tropomodulin 3,ubiquitous

    miRNA
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    miRTarBase miRNAs that target TMOD3:
    hsa-mir-145 (MIRT001811)

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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat TMOD3
    8/33 QIAGEN miScript miRNA Assays for microRNAs that regulate TMOD3 (see all 33):
    hsa-miR-411* hsa-miR-3130-5p hsa-miR-379* hsa-miR-429 hsa-miR-29a hsa-miR-3653 hsa-miR-138-2* hsa-miR-570
    SwitchGear 3'UTR luciferase reporter plasmidTMOD3 3' UTR sequence
    Inhib. RNA
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    OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for TMOD3 (see all 7)
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003779actin binding IEA--
    GO:0005523tropomyosin binding IEA--


    TMOD3 for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TMOD3

    STRING Interaction Network Preview (showing 5 interactants - click image to see 10)

    5/543 Interacting proteins for TMOD3 (Q9NYL92, 3 ENSP000003087534) via UniProtKB, MINT, STRING, and/or I2D (see all 543)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DUSP23Q9BVJ72, 3MINT-8254772 I2D: score=2 
    MAP1LC3BQ9GZQ82, 3MINT-8259822 I2D: score=2 
    MAP3K3Q997592, 3MINT-48381 I2D: score=2 
    TNFSF11O147882, 3MINT-8274775 I2D: score=2 
    GRB2P629933, ENSP000003390074I2D: score=1 STRING: ENSP00000339007
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TMOD3
    Search CenterWatch for drugs/clinical trials and news about TMOD3 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TMOD3 gene: 
    NM_014547.4  

    Unigene Cluster for TMOD3:

    Tropomodulin 3 (ubiquitous)
    Hs.4998  [show with all ESTs]
    Unigene Representative Sequence: NM_014547
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000558455 ENST00000308580(uc002abn.3) ENST00000560549(uc010bfc.1)
    ENST00000561438 ENST00000560725 ENST00000558714 ENST00000559202 ENST00000558666
    ENST00000561408 ENST00000561136 ENST00000558300 ENST00000544199

    miRNA
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    8/33 QIAGEN miScript miRNA Assays for microRNAs that regulate TMOD3 (see all 33):
    hsa-miR-411* hsa-miR-3130-5p hsa-miR-379* hsa-miR-429 hsa-miR-29a hsa-miR-3653 hsa-miR-138-2* hsa-miR-570
    SwitchGear 3'UTR luciferase reporter plasmidTMOD3 3' UTR sequence
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    Additional cDNA sequence: 

    AF177171.1 AF237631.1 AK025704.1 AK310344.1 AK312569.1 AL137543.1 BC020542.2 BX647277.1 

    10 DOTS entries:

    DT.95288682  DT.100788876  DT.121037836  DT.95288667  DT.92023806  DT.121644615  DT.95253715  DT.121037833 
    DT.86997854  DT.91721830 

    24/181 AceView cDNA sequences (see all 181):

    BU618675 BQ000126 AA327339 CA422807 AV682778 AI433810 BU616978 AA203464 
    CA417754 BI870718 BU616792 AK025704 BU620795 AI186900 BX647277 AW576360 
    AA327338 BQ774182 BI760796 CB243008 BE888181 BM983040 BG675975 CB144650 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TMOD3 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: --

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    TMOD3 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    4 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    KidneyPodocyte LayerPodocytesKidney
    Skeletal MuscleHyoid Arch MusclesMyoblastsSkeletal Muscle
    Skeletal MuscleMandibular Arch MusclesMyoblastsSkeletal Muscle
    BoneThoracic RibBone
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See TMOD3 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TMOD3

    SOURCE GeneReport for Unigene cluster: Hs.4998

    UniProtKB/Swiss-Prot: TMOD3_HUMAN, Q9NYL9
    Tissue specificity: Ubiquitous

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TMOD3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for TMOD3 gene from 7/21 species (see all 21)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Tmod31 , 5 tropomodulin 31, 5 86.65(n)1
    90.06(a)1
      9 (42.30 cM)5
    508751  NM_016963.21  NP_058659.11 
     754977965 
    chicken
    (Gallus gallus)
    Aves TMOD31 tropomodulin 3 (ubiquitous) 79.92(n)
    82.1(a)
      415421  NM_001005813.1  NP_001005813.1 
    lizard
    (Anolis carolinensis)
    Reptilia TMOD36
    --
    84(a)
    1 ↔ 1
    GL343330.1(782516-805983)
    African clawed frog
    (Xenopus laevis)
    Amphibia tmod3-prov2 tropomodulin 3 79.73(n)    BC047163.1 
    zebrafish
    (Danio rerio)
    Actinopterygii tmod26
    tropomodulin 2
    72(a)
    1 → many
    18(37764299-37826745)
    fruit fly
    (Drosophila melanogaster)
    Insecta spdo3 peripheral nervous system development actin
    binding
    33(a)     --
    worm
    (Caenorhabditis elegans)
    Secernentea unc-946
    tmd-26
    TropoMoDulin family member (tmd-2)
    30(a)
    10(a)
    many ↔ many
    many ↔ many
    I(5992672-6001101)
    V(8336638-8340655)


    ENSEMBL Gene Tree for TMOD3 (if available)
    TreeFam Gene Tree for TMOD3 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TMOD3 gene
    LMOD32  TMOD12  LMOD22  TMOD42  LMOD12  TMOD22  
    7 SIMAP similar genes for TMOD3 using alignment to 4 protein entries:     TMOD3_HUMAN (see all proteins):
    DKFZp686E1899    TMOD2    TMOD1    TMOD4    LMOD1    LMOD2
    LMOD3

    TMOD3 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1503 NCBI SNPs in TMOD3 are shown (see all 1503    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1171642891,2
    F,--52120052(+) ATGAGA/GTTGTC 1 -- us2k11Minor allele frequency- G:0.06EA 120
    rs1876151831,2
    --52120227(+) CTTATA/CTATAG 1 -- us2k10--------
    rs1920954961,2
    --52120238(+) AAATGG/TAGGAT 1 -- us2k10--------
    rs116378761,2
    C,F,A,H,--52120393(+) CTCAGA/GAAGCT 1 -- us2k126Minor allele frequency- G:0.35NS EA NA WA CSA 2558
    rs177057321,2
    C,F,H,--52120428(+) AGATGG/ATATAG 1 -- us2k1 tfbs320Minor allele frequency- A:0.26NA NS EA WA 2480
    rs1452595191,2
    --52120481(+) TCTAAA/GTATTT 1 -- us2k10--------
    rs1487077751,2
    --52120512(+) CCTGGC/TCAAGC 1 -- us2k10--------
    rs1850773441,2
    --52120576(+) CATGAA/GGTCAA 1 -- us2k10--------
    rs1422763331,2
    --52120617(+) AAAACC/TCATCT 1 -- us2k10--------
    rs1894525591,2
    --52120660(+) TGGCAC/TGTGCC 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for TMOD3 (52121825 - 52239492 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for TMOD3: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TMOD3
    DNA2.0 Custom Variant and Variant Library Synthesis for TMOD3

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TMOD3 for disorders           About GeneDecksing

    OMIM gene information: 605112    OMIM disorders: --

    5 diseases for TMOD3:    About MalaCards
    estrogen excess    transitional cell carcinoma    pulmonary hypertension    hypertension
    carcinoma


    Export disorders for TMOD3 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TMOD3 gene, integrated from 9 sources (see all 36):
    (articles sorted by number of sources associating them with TMOD3)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Sequencing, expression analysis, and mapping of three unique human tropomodulin genes and their mouse orthologs. (PubMed id 10662549)1, 2, 3, 9 Cox P.R. and Zoghbi H.Y. (2000)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Tropomodulin 3 binds to actin monomers. (PubMed id 17012745)1, 9 Fischer R.S....Fowler V.M. (2006)
    4. Pointed-end capping by tropomodulin3 negatively regulates endothelial cell motility. (PubMed id 12707310)1, 9 Fischer R.S....Fowler V.M. (2003)
    5. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    6. A high-throughput approach for measuring temporal chan ges in the interactome. (PubMed id 22863883)1 Kristensen A.R....Foster L.J. (2012)
    7. Identification and validation of novel serum markers f or early diagnosis of endometriosis. (PubMed id 22158085)1 Gajbhiye R....Khole V. (2012)
    8. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    9. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    10. A directed protein interaction network for investigat ing intracellular signal transduction. (PubMed id 21900206)1 Vinayagam A....Wanker E.E. (2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 29766 HGNC: 11873 AceView: TMOD3 Ensembl:ENSG00000138594 euGenes: HUgn29766
    ECgene: TMOD3 H-InvDB: TMOD3

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TMOD3 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TMOD3 gene:
    Search GeneIP for patents involving TMOD3

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
    In Situ Hybridization Assays from
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    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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