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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TMEM86B Gene

protein-coding   GIFtS: 43
GCID: GC19M055738

transmembrane protein 86B

  Search for TMEM86B
in our new
 Human Malady Compendium 
Biological research products
for TMEM86B
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Transmembrane Protein 86B1 2 3
MGC302081
Lysoplasmalogenase1
EC 3.3.2.23
EC 3.3.2.53

External Ids:    HGNC: 284481   Entrez Gene: 2550432   Ensembl: ENSG000001800897   UniProtKB: Q8N6613   

Export aliases for TMEM86B gene to outside databases

Previous GC identifers: GC19M060430 GC19M052061


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: TM86B_HUMAN, Q8N661
Function: Enzyme catalyzing the degradation of lysoplasmalogen. Lysoplasmalogens are formed by the hydrolysis of the
abundant membrane glycerophospholipids plasmalogens. May control the respective levels of plasmalogens and
lysoplasmalogens in cells and modulate cell membrane properties




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000019.9  NC_018930.1  NT_011109.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TMEM86B gene promoter:
         NRSF form 1   MyoD   POU6F1 (c2)   HNF-4alpha2   NRSF form 2   FOXL1   c-Ets-1   HNF-4alpha1   STAT3   MRF-2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTMEM86B promoter sequence
   Search SABiosciences Chromatin IP Primers for TMEM86B

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TMEM86B


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.42   Ensembl cytogenetic band:  19q13.42   HGNC cytogenetic band: 19q13.42

TMEM86B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TMEM86B gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M055738:  view genomic region     (about GC identifiers)

Start:
55,738,002 bp from pter      End:
55,741,647 bp from pter
Size:
3,646 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TM86B_HUMAN, Q8N661 (See protein sequence)
Recommended Name: Lysoplasmalogenase  
Size: 226 amino acids; 24352 Da
Subunit: Homodimer (Probable)
Subcellular location: Membrane; Multi-pass membrane protein (Potential). Cytoplasm

Explore the universe of human proteins at neXtProt for TMEM86B: NX_Q8N661

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q8N661

  • TMEM86B Protein expression data from MOPED and PaxDb:    About this image 
    TMEM86B Protein Expression
    REFSEQ proteins: NP_776165.3  
    ENSEMBL proteins: 
     ENSP00000321038  

    Human Recombinant Protein Products for TMEM86B: 
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    Uscn Proteins for TMEM86B

    Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0016020membrane IDA--
    GO:0016021integral to membrane IEA--
    GO:0044444cytoplasmic part IDA--

    TMEM86B for ontologies           About GeneDecksing



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    Uscn ELISAs and CLIAs for TMEM86B


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TMEM86B for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR012506 YhhN

    Graphical View of Domain Structure for InterPro Entry Q8N661

    ProtoNet protein and cluster: Q8N661

    1 Blocks protein family: IPB012506 YhhN-like

    UniProtKB/Swiss-Prot: TM86B_HUMAN, Q8N661
    Similarity: Belongs to the TMEM86 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: TM86B_HUMAN, Q8N661
    Function: Enzyme catalyzing the degradation of lysoplasmalogen. Lysoplasmalogens are formed by the hydrolysis of the
    abundant membrane glycerophospholipids plasmalogens. May control the respective levels of plasmalogens and
    lysoplasmalogens in cells and modulate cell membrane properties
    Catalytic activity: 1-(1-alkenyl)-sn-glycero-3-phosphocholine + H(2)O = an aldehyde + sn-glycero-3-phosphocholine
    Catalytic activity: 1-(1-alkenyl)-sn-glycero-3-phosphoethanolamine + H(2)O = an aldehyde +
    sn-glycero-3-phosphoethanolamine
    Enzyme regulation: Competitively inhibited by lysophosphatidic acid
    Biophysicochemical properties: Kinetic parameters: KM=45 uM for lysoplasmenyl-choline; KM=53 uM for
    lysoplasmenyl-ethanolamine; pH dependence: Optimum pH is 6.95 and 7.05 with lysoplasmenyl-ethanolamine and
    lysoplasmenyl-choline as substrates, respectively;

         Enzyme Numbers (IUBMB): EC 3.3.2.21 EC 3.3.2.51

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0047408alkenylglycerophosphocholine hydrolase activity IDA--
    GO:0047409alkenylglycerophosphoethanolamine hydrolase activity IDA--
         
    TMEM86B for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for TMEM86B:
     Synthetic lethal with Ras 

    Animal Models:
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for TMEM86B 

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    In Situ Assay
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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TMEM86B

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for TMEM86B (ENSP000003210384) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HDAC8ENSP000003626744STRING: ENSP00000362674
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0046485ether lipid metabolic process IDA--

    TMEM86B for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TMEM86B
    Search CenterWatch for drugs/clinical trials and news about TMEM86B / TM86B 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TMEM86B gene: 
    NM_173804.4  

    Unigene Cluster for TMEM86B:

    Transmembrane protein 86B
    Hs.135215  [show with all ESTs]
    Unigene Representative Sequence: AK127755
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000589190 ENST00000327042(uc002qjt.3 uc002qju.3) ENST00000585416
    ENST00000586923

    miRNA
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    Inhib. RNA
    Products:
         
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    OriGene shRNA RFP: TMEM86B
    OriGene siRNA: TMEM86B
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    Sirion Biotech Custom design and validation of potent shRNA sequences against TMEM86B 
    Clone
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    OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TMEM86B
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TMEM86B
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    Additional cDNA sequence: 

    AK127755.1 BC023000.1 

    3 DOTS entries:

    DT.100019965  DT.423689  DT.446411 

    24/362 AceView cDNA sequences (see all 362):

    BM824717 BQ677114 BQ682703 BM810799 CR595325 BE513401 BQ888296 CB155253 
    AL703312 BQ063473 AL040848 CO246210 BQ064215 BQ920736 BM786186 CB852961 
    BQ054504 CF125931 CN479984 BM725215 BM449832 AA689488 BI868429 BQ226010 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TMEM86B expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTCACTGTCG
    TMEM86B Expression
    About this image

    TMEM86B expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table

    Stem Cell Differentiation: 1 LifeMap Cell 
    NameCategory
    Late limb mesenchymal cells (LLM cells) (Primary Cell)Bone, Cartilage, Limb
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See TMEM86B Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TMEM86B

    SOURCE GeneReport for Unigene cluster: Hs.135215
        SABiosciences Custom PCR Arrays for TMEM86B
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TMEM86B

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for TMEM86B gene from 4/17 species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Tmem86b1 , 5 transmembrane protein 86B1, 5 75.26(n)1
    66.22(a)1
      7 (2.67 cM)5
    682551  NM_023440.21  NP_075929.11 
     46280425 
    lizard
    (Anolis carolinensis)
    Reptilia TMEM86B6
    --
    37(a)
    1 ↔ 1
    GL343355.1(1008714-1013802)
    zebrafish
    (Danio rerio)
    Actinopterygii tmem86b6
    transmembrane protein 86B
    29(a)
    1 ↔ 1
    3(16382464-16390299)
    fruit fly
    (Drosophila melanogaster)
    Insecta CG75826
    --
    27(a)
    1 → many
    3R(25505963-25508000)


    ENSEMBL Gene Tree for TMEM86B (if available)
    TreeFam Gene Tree for TMEM86B (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TMEM86B gene
    TMEM86A2  
    1 SIMAP similar gene for TMEM86B using alignment to 1 protein entry:     TM86B_HUMAN:
    TMEM86A

    TMEM86B for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/76 NCBI SNPs in TMEM86B are shown (see all 76    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1899625031,2
    --55737553(+) TTGTAC/TCCCCT 1 -- ds50010--------
    rs1830818171,2
    --55737559(+) CCCCTA/GGAAAG 1 -- ds50010--------
    rs1884631881,2
    --55737723(+) ACAGAA/GGGAGG 1 -- ds50010--------
    rs1929269831,2
    --55737750(+) GTCACA/GTGAAG 1 -- ds50010--------
    rs1403225411,2
    --55737758(+) AAGACA/GGAGAG 1 -- ds50010--------
    rs561549251,2
    C--55737798(+) ATTCCC/TGGGAG 1 -- ds50014Minor allele frequency- T:0.14WA NA EA 360
    rs93047641,2
    C,F,A,H--55737816(+) AAGCCG/AAGAGG 1 -- ds500121Minor allele frequency- A:0.44NS EA NA WA CSA 2048
    rs1170858601,2
    --55737861(+) GACCAT/CGGGCC 1 -- ds50011Minor allele frequency- C:0.01NA 120
    rs1380801941,2
    --55737878(+) CACCCA/GGATTT 1 -- ds50010--------
    rs1416330911,2
    --55738103(+) AGCGAC/TAAAGA 1 -- ut310--------

    HapMap Linkage Disequilibrium report for TMEM86B (55738002 - 55741647 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for TMEM86B: --

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TMEM86B
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TMEM86B gene integrated from 9 sources:
    (articles sorted by number of sources associating them with TMEM86B)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen. (PubMed id 21515882)1, 2, 3 Wu L.C....Jurkowitz M.S. (2011)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Functional dissection of lysine deacetylases reveals t hat HDAC1 and p300 regulate AMPK. (PubMed id 22318606)1 Lin Y.Y....Boeke J.D. (2012)
    4. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (2007)
    5. The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)2 Grimwood J.... Lucas S.M. (2004)
    6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 255043 HGNC: 28448 AceView: MGC30208andKIAA1115 Ensembl:ENSG00000180089 euGenes: HUgn255043
    ECgene: TMEM86B H-InvDB: TMEM86B

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TMEM86B Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TMEM86B gene:
    Search GeneIP for patents involving TMEM86B

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
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    GeneCards Homepage - Last full update: 18 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 13 May 2013

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    VWF
    (GIFTS: 73)
    von Willebrand factor
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