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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TMEM65 Gene

protein-coding   GIFtS: 42
GCID: GC08M125394

Transmembrane Protein 65

  Search for TMEM65
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Transmembrane Protein 651 2

External Ids:    HGNC: 252031   Entrez Gene: 1573782   Ensembl: ENSG000001649837   UniProtKB: Q6PI783   

Export aliases for TMEM65 gene to outside databases

Previous GC identifer: GC08M120650


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

GeneCards Summary for TMEM65 Gene: 
TMEM65 (transmembrane protein 65) is a protein-coding gene.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000008.10  NT_008046.16  NC_018919.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TMEM65 gene promoter:
         ER-alpha   AhR   ISGF-3   FOXF2   Pbx1a   POU3F1   HNF-3beta   NF-AT   Arnt   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTMEM65 promoter sequence
   Search SABiosciences Chromatin IP Primers for TMEM65

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TMEM65


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24.13   Ensembl cytogenetic band:  8q24.13   HGNC cytogenetic band: 8q24.13

TMEM65 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TMEM65 gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08M125394:  view genomic region     (about GC identifiers)

Start:
125,323,159 bp from pter      End:
125,384,940 bp from pter
Size:
61,782 bases      Orientation:
minus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: TMM65_HUMAN, Q6PI78 (See protein sequence)
Recommended Name: Transmembrane protein 65  
Size: 240 amino acids; 25498 Da
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
Sequence caution: Sequence=AAH32396.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: Q8N5G8 Q8WVK5

Explore the universe of human proteins at neXtProt for TMEM65: NX_Q6PI78

Explore proteomics data for TMEM65 at MOPED 

TMEM65 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

TMEM65 Protein Expression

REFSEQ proteins: NP_919267.2  
ENSEMBL proteins: 
 ENSP00000297632  

Human Recombinant Protein Products for TMEM65: 
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Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005739mitochondrion IEA--
GO:0016021integral to membrane IEA--

TMEM65 for ontologies           About GeneDecksing



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Cloud-Clone Corp. CLIAs for TMEM65


(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section
1 InterPro protein domain:
 IPR019537 Transmembrane_protein_65

Graphical View of Domain Structure for InterPro Entry Q6PI78

ProtoNet protein and cluster: Q6PI78


TMEM65 for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
About This Section

Animal Models:
   inGenious Targeting Laboratory - Custom generated mouse model solutions for TMEM65 
   inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for TMEM65

   genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for TMEM65 
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SwitchGear 3'UTR luciferase reporter plasmidTMEM65 3' UTR sequence
Inhib. RNA
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(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
About This Section



Interactions:

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TMEM65

STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

1 Interacting protein for TMEM65 (ENSP000002976324) via UniProtKB, MINT, STRING, and/or I2D
InteractantInteraction Details
GeneCardExternal ID(s)
ELAVL1ENSP000003852694STRING: ENSP00000385269
About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section
Browse Small Molecules at EMD Millipore
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for TMEM65 (TMM65)

Search CenterWatch for drugs/clinical trials and news about TMEM65 / TMM65

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for TMEM65 gene: 
NM_194291.2  

Unigene Cluster for TMEM65:

Transmembrane protein 65
Hs.187646  [show with all ESTs]
Unigene Representative Sequence: NM_194291
1 Ensembl transcript including schematic representation, and UCSC links where relevant:
ENST00000297632(uc010mdl.3)
miRNA
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hsa-miR-323-3p hsa-miR-607 hsa-miR-640 hsa-let-7d hsa-miR-29a hsa-miR-550a* hsa-miR-374c hsa-let-7g
SwitchGear 3'UTR luciferase reporter plasmidTMEM65 3' UTR sequence
Inhib. RNA
Products:
     
Browse for Gene Knock-down Tools from EMD Millipore
OriGene RNAi products in human, mouse, rat for TMEM65
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Clone
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Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TMEM65
Sirion Biotech Customized lentivirus for stable overexpression of TMEM65 
                     Customized lentivirus expression plasmids for stable overexpression of TMEM65 
Primer
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  QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TMEM65
  QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TMEM65

Additional mRNA sequence: 

BC017881.1 BC032396.1 BC041379.1 

5 DOTS entries:

DT.100685402  DT.121485300  DT.100653494  DT.95368251  DT.433409 

24/62 AceView cDNA sequences (see all 62):

BM055024 AA318471 BQ433192 BC017881 BM055302 AA485369 BF594757 AI628363 
BE047139 AI797245 CR610944 AW300050 CK904892 CK904893 AA594779 CA848355 
BM679703 BX402212 BX431036 BC032396 CF552417 D60949 NM_194291 CA848650 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

TMEM65 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: --
TMEM65 Expression
About this image


See TMEM65 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TMEM65

SOURCE GeneReport for Unigene cluster: Hs.187646
    SABiosciences Custom PCR Arrays for TMEM65
Primer
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QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TMEM65
QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TMEM65
In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TMEM65

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for TMEM65 gene from 7/14 species (see all 14)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Tmem651 , 5 transmembrane protein 651, 5 91.95(n)1
95.26(a)1
  15 (25.09 cM)5
748681  NM_175212.41  NP_780421.21 
 587822695 
chicken
(Gallus gallus)
Aves TMEM651 transmembrane protein 65 84.66(n)
87.5(a)
  428387  NM_001031507.1  NP_001026678.1 
lizard
(Anolis carolinensis)
Reptilia TMEM656
Uncharacterized protein
74(a)
1 ↔ 1
4(6324371-6350323)
African clawed frog
(Xenopus laevis)
Amphibia Xl.139832 Xenopus laevis transcribed sequence with weak similarity more 77.68(n)    48051019 
zebrafish
(Danio rerio)
Actinopterygii tmem651 transmembrane protein 65 63.79(n)
66.5(a)
  100005403  NM_001128812.1  NP_001122284.1 
fruit fly
(Drosophila melanogaster)
Insecta CG177151 CG17715 52.03(n)
46.34(a)
  3355129  NM_001042943.2  NP_001036408.1 
worm
(Caenorhabditis elegans)
Secernentea tag-3216
Protein TAG-321
38(a)
1 ↔ 1
IV(7782598-7783853)


ENSEMBL Gene Tree for TMEM65 (if available)
TreeFam Gene Tree for TMEM65 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
  --

(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

10/1141 SNPs in TMEM65 are shown (see all 1141)    About this table     
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 8 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs1426491181,2
C--125322672(+) TGATG-/TCCC  
        
TCCCT
1 -- ds50010--------
rs792687751,2
--125322702(+) GACAGG/TCTTCA 1 -- ds50011Minor allele frequency- T:0.01WA 118
rs1857013551,2
--125322743(+) TATTCA/GTAGTG 1 -- ds50010--------
rs1903676001,2
--125322957(+) GATCCA/TAAGCC 1 -- ds50010--------
rs127079071,2
C,F,A,H--125323011(+) ACCAGT/CTTCTA 1 -- ds500120Minor allele frequency- C:0.09NS EA NA 2074
rs1827792921,2
--125323149(+) ACATAA/GAGATA 1 -- ds50010--------
rs1469948421,2
C--125323260(+) GCTCAC/TACAAA 1 -- ut310--------
rs801866821,2
C,F--125323295(+) CATTAC/TCTTCA 1 -- ut313Minor allele frequency- T:0.06WA NA EA 358
rs1380862961,2
--125323322(+) GAAAAA/GGAATA 1 -- ut310--------
rs1874940091,2
--125323442(+) CATACA/GCTGAT 1 -- ut310--------

HapMap Linkage Disequilibrium report for TMEM65 (125323159 - 125384940 bp)

Structural Variations
     Database of Genomic Variants (DGV) 4 variations for TMEM65:    About this table     
Variant IDTypeSubtypePubMed ID
esv2669472CNV Deletion23128226
nsv521356CNV Loss19592680
nsv527751CNV Loss19592680
nsv516190CNV Loss19592680

SABiosciences Cancer Mutation PCR Assays
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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

TMEM65 for disorders           About MalaCards

TMEM65 for disorders           About GeneDecksing

Genetic Association Database (GAD): TMEM65
Human Genome Epidemiology (HuGE) Navigator: TMEM65 (1 document)

Export disorders for TMEM65 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
About This Section

PubMed articles for TMEM65 gene integrated from 9 sources:
(articles sorted by number of sources associating them with TMEM65)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Genetic variants in nuclear-encoded mitochondrial gen es influence AIDS progression. (PubMed id 20877624)1, 4 Hendrickson S.L....O'Brien S.J. (2010)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  3. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (2002)
  4. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
  5. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
  6. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (2009)
  7. DNA sequence and analysis of human chromosome 8. (PubMed id 16421571)2 Nusbaum C....Lander E.S. (2006)
  8. Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)1 Bonaldo M.F.... Soares M.B. (1996)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 157378 HGNC: 25203 AceView: LOC157378 Ensembl:ENSG00000164983 euGenes: HUgn157378
ECgene: TMEM65 H-InvDB: TMEM65

(According to HUGE)
About This Section
  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
PharmGKB entry for TMEM65 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
About This Section
Patent Information for TMEM65 gene:
Search GeneIP for patents involving TMEM65

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
About This Section

 
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 Browse Sino Biological Proteins and Antibodies
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 Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
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 See all of Abcam's Antibodies, Kits and Proteins for TMEM65
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 Proteins for TMEM65
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 Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TMEM65
 SwitchGear 3'UTR luciferase reporter plasmids for TMEM65
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 ThermoFisher Antibodies for TMEM65
 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TMEM65
 inGenious Targeting Laboratory - Custom generated mouse model solutions for TMEM65
 inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for TMEM65
Customized:
 lentivirus for stable overexpression of TMEM65
 lentivirus expression plasmids for stable overexpression of TMEM65
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Customized transgenic rodents for:
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GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 3 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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