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TMEM174 Gene

protein-coding   GIFtS: 41
GCID: GC05P072499

Transmembrane Protein 174

  See related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Transmembrane Protein 1741 2

External Ids:    HGNC: 281871   Entrez Gene: 1342882   Ensembl: ENSG000001643257   OMIM: 6149095   UniProtKB: Q8WUU83   

Export aliases for TMEM174 gene to outside databases

Previous GC identifer: GC05P067674


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for TMEM174 Gene:
TMEM174 (transmembrane protein 174) is a protein-coding gene. Diseases associated with TMEM174 include crohn's disease.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000005.10  NT_034772.7  NC_018916.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the TMEM174 gene promoter:
         GR   GR-beta   HNF-1A   C/EBPalpha   E2F-1   E2F   HNF-1   GR-alpha   Pax-4a   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTMEM174 promoter sequence
   Search Chromatin IP Primers for TMEM174

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TMEM174


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q13.2   Ensembl cytogenetic band:  5q13.2   HGNC cytogenetic band: 5q13.2

TMEM174 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TMEM174 gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P072499:  view genomic region     (about GC identifiers)

Start:
72,469,022 bp from pter      End:
72,470,970 bp from pter
Size:
1,949 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: TM174_HUMAN, Q8WUU8 (See protein sequence)
Recommended Name: Transmembrane protein 174  
Size: 243 amino acids; 26287 Da
Secondary accessions: B2RDA0 Q96N81
Alternative splicing: 2 isoforms:  Q8WUU8-1   Q8WUU8-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for TMEM174: NX_Q8WUU8

REFSEQ proteins: NP_694949.1  
ENSEMBL proteins: 
 ENSP00000296776  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro protein domain:
 IPR027835 TMEM174

Graphical View of Domain Structure for InterPro Entry Q8WUU8

ProtoNet protein and cluster: Q8WUU8


TMEM174 for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Animal Models:
   inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for TMEM174
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SwitchGear 3'UTR luciferase reporter plasmidTMEM174 3' UTR sequence
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot
TM174_HUMAN, Q8WUU8: Endoplasmic reticulum membrane; Multi-pass membrane protein
Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
endoplasmic reticulum5
plasma membrane3
nucleus1

Gene Ontology (GO): 2 cellular component terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005789endoplasmic reticulum membrane IEA--
GO:0016021integral component of membrane IEA--

TMEM174 for ontologies           About GeneDecksing


(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for TMEM174
Interactions:

    Search GeneGlobe Interaction Network for TMEM174

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for TMEM174 (TM174)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for TMEM174 gene: 
NM_153217.2  

Unigene Cluster for TMEM174:

Transmembrane protein 174
Hs.508588  [show with all ESTs]
Unigene Representative Sequence: NM_153217
2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000296776(uc010izc.3) ENST00000511737
miRNA
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hsa-miR-647 hsa-miR-298 hsa-miR-323b-3p hsa-miR-1236 hsa-miR-516a-3p hsa-miR-516b*
SwitchGear 3'UTR luciferase reporter plasmidTMEM174 3' UTR sequence
Inhib. RNA
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  QuantiFast Probe-based Assays in human, mouse, rat TMEM174

Additional mRNA sequence: 

AK055830.1 AK315460.1 BC019346.1 

4 DOTS entries:

DT.204701  DT.97821613  DT.95266481  DT.95266482 

Selected AceView cDNA sequences (see all 39):

AW051725 BF197319 AI732994 AI791388 AI820803 BC019346 BI759355 AI792469 
BX098359 BI759876 AI733632 NM_153217 AI017691 AI478174 BF594633 BI762280 
BF196449 AI222525 AW243279 AK055830 AW299613 AA864848 BE048613 BG428939 

GeneLoc Exon Structure

2 Alternative Splicing Database (ASD) splice patterns (SP) for TMEM174    About this scheme

ExUns: 1a · 1b ^ 2
SP1:                  
SP2:                  


ECgene alternative splicing isoforms for TMEM174

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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TMEM174 expression in normal human tissues (normalized intensities)      TMEM174 embryonic expression: see
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: TCAAGTCCTC
TMEM174 Expression
About this image


TMEM174 expression in embryonic tissues and stem cells    About this table
Data from LifeMap, the Embryonic Development and Stem Cells Database
 selected tissues (see all 3) fully expand
 
 Kidney (Urinary System)
         Presumptive Podocytes Podocyte Layer
 
 Epithelial Cells
         Presumptive Podocytes Podocyte Layer
 
 Ovary (Reproductive System)
TMEM174 Protein expression data from MOPED1, PaxDb2 and MaxQB3 --

SOURCE GeneReport for Unigene cluster: Hs.508588

UniProtKB/Swiss-Prot: TM174_HUMAN, Q8WUU8
Tissue specificity: Predominantly expressed in kidney

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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TMEM174

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of chordates.

Orthologs for TMEM174 gene from Selected species (see all 10)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Tmem1741 , 5 transmembrane protein 1741, 5 82.85(n)1
81.48(a)1
  13 (52.11 cM)5
683441  NM_026685.21  NP_080961.11 
 986349785 
chicken
(Gallus gallus)
Aves TMEM1741 transmembrane protein 174 64.18(n)
58.92(a)
  768886  XM_001231594.3  XP_001231595.1 
lizard
(Anolis carolinensis)
Reptilia TMEM1746
transmembrane protein 174
46(a)
1 ↔ 1
2(16127880-16130330)
tropical clawed frog
(Xenopus tropicalis)
Amphibia tmem1741 transmembrane protein 174 62.3(n)
63.35(a)
  100038281  XM_004910822.1  XP_004910879.1 
zebrafish
(Danio rerio)
Actinopterygii LOC1018858561 transmembrane protein 174-like 57.69(n)
54.34(a)
  101885856  XM_005171978.1  XP_005172035.1 


ENSEMBL Gene Tree for TMEM174 (if available)
TreeFam Gene Tree for TMEM174 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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  --

(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for TMEM174 (see all 139)    About this table    
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 5 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs731039191,2
C,F--72467076(+) GTTCCA/CGTTTG 1 -- us2k12Minor allele frequency- C:0.04WA 120
rs1871245321,2
--72467160(+) TATAGC/TTAAAG 1 -- us2k10--------
rs1906512951,2
--72467168(+) AAGGCA/GAATGG 1 -- us2k10--------
rs1140740461,2
F--72467190(+) TGAGTG/TATTTA 1 -- us2k11Minor allele frequency- T:0.01WA 118
rs1151686781,2
F--72467258(+) AACTTA/CGGAGT 1 -- us2k11Minor allele frequency- C:0.04WA 118
rs771628721,2
F--72467496(+) GTCCTG/CTAGCA 1 -- us2k11Minor allele frequency- C:0.02WA 118
rs1169544771,2
F--72467680(+) TGGCCC/TATGTG 1 -- us2k11Minor allele frequency- T:0.01EA 120
rs1828674221,2
--72467740(+) CAGCCC/TTCTCA 1 -- us2k10--------
rs1136883911,2
C,F--72467877(+) TGGAAG/CTTTTG 1 -- us2k12Minor allele frequency- C:0.50CSA 4
rs779575111,2
C,F--72467937(+) CATCAA/GCTTGG 1 -- us2k11Minor allele frequency- G:0.06WA 118

HapMap Linkage Disequilibrium report for TMEM174 (72469022 - 72470970 bp)

Structural Variations
     Database of Genomic Variants (DGV) 2 variations for TMEM174:    About this table    
Variant IDTypeSubtypePubMed ID
nsv4873CNV Insertion18451855
nsv830344CNV Gain+Loss17160897

Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing TMEM174
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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 614909    OMIM disorders: --

1 disease for TMEM174:    About MalaCards
crohn's disease


TMEM174 for disorders           About GeneDecksing

Genetic Association Database (GAD): TMEM174

Export disorders for TMEM174 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for TMEM174 gene integrated from 10 sources:
(articles sorted by number of sources associating them with TMEM174)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Human TMEM174 that is highly expressed in kidney tissue activates AP- 1 and promotes cell proliferation. (PubMed id 20331980)1, 2 Wang P.... Ma D. (Biochem. Biophys. Res. Commun. 2010)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
  4. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  5. Bcl2-associated athanogene 3 interactome analysis reveals a new role in modulating proteasome activity. (PubMed id 23824909)1 Chen Y....Ge F. (amp 2013)
  6. Meta-analysis identifies multiple loci associated with kidney function-related traits in east Asian populations. (PubMed id 22797727)1 Okada Y....Tanaka T. (Nat. Genet. 2012)
  7. Genome-wide meta-analysis increases to 71 the number of confirmed Crohn's disease susceptibility loci. (PubMed id 21102463)4 Franke A....Parkes M. (Nat. Genet. 2010)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 134288 HGNC: 28187 AceView: MGC13034 Ensembl:ENSG00000164325 euGenes: HUgn134288
ECgene: TMEM174 H-InvDB: TMEM174

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for TMEM174 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for TMEM174 gene:
Search GeneIP for patents involving TMEM174

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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 CLIAs for TMEM174



 Browse ESI BIO Cell Lines and PureStem Progenitors for TMEM174
 Gene Synthesis
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 Variant Library Synthesis
 Codon Optimization
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 Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TMEM174
 SwitchGear 3'UTR luciferase reporter plasmids for TMEM174
 SwitchGear Promoter luciferase reporter plasmids for TMEM174
 Search ThermoFisher Antibodies for TMEM174
 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TMEM174
 inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for TMEM174
 inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for TMEM174
 Search LSBio for Antibodies for TMEM174
Customized transgenic rodents for:
 Humanization
 Biomarker expression
 Off-target effect monitoring
 Translational medicine
 Tissue-specific gene expresssion
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 Browse compounds at ApexBio
       
GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

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