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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TMEM132A Gene

protein-coding   GIFtS: 45
GCID: GC11P060691

transmembrane protein 132A

(Previous names: heat shock 70kDa protein 5 (glucose-regulated protein,...)
(Previous symbol: HSPA5BP1)
 Explore 1 disease affiliated with
TMEM132A via our new
 Human Malady Compendium 
Biological research products
for TMEM132A
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Transmembrane Protein 132A1 2     FLJ205391
HSPA5BP11 2 3     Glucose-Regulated Protein, 78kDa2
GBP1 2     GRP78-Binding Protein2
Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) Binding Protein 11 2     Heat Shock 70kDa Protein 5 Binding Protein 12
HSPA5-Binding Protein 12 3     KIAA15833

External Ids:    HGNC: 310921   Entrez Gene: 549722   Ensembl: ENSG000000061187   UniProtKB: Q24JP53   

Export aliases for TMEM132A gene to outside databases

Previous GC identifers: GC11P060449 GC11P057038


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TMEM132A:
This gene encodes a protein that is highly similar to the rat Grp78-binding protein (GBP). Alternatively spliced
transcript variants encoding different isoforms have been described. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: T132A_HUMAN, Q24JP5
Function: May play a role in embryonic and postnatal development of the brain. Increased resistance to cell death
induced by serum starvation in cultured cells. Regulates cAMP-induced GFAP gene expression via STAT3 phosphorylation
(By similarity)




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000011.9  NC_018922.1  NT_167190.1  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TMEM132A gene promoter:
         CREB   deltaCREB   Olf-1   HNF-4alpha2   AP-4   HNF-4alpha1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTMEM132A promoter sequence
   Search SABiosciences Chromatin IP Primers for TMEM132A

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TMEM132A


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q12.2   Ensembl cytogenetic band:  11q12.2   HGNC cytogenetic band: 11q12.2

TMEM132A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TMEM132A gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P060691:  view genomic region     (about GC identifiers)

Start:
60,691,913 bp from pter      End:
60,704,631 bp from pter
Size:
12,719 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: T132A_HUMAN, Q24JP5 (See protein sequence)
Recommended Name: Transmembrane protein 132A precursor  
Size: 1023 amino acids; 110110 Da
Subunit: Interacts with HSPA5/GRP78 (By similarity)
Subcellular location: Golgi apparatus membrane; Single-pass type I membrane protein (By similarity). Endoplasmic
reticulum membrane; Single-pass type I membrane protein (By similarity)
Sequence caution: Sequence=BAB13409.1; Type=Erroneous initiation; Sequence=BAB14613.1; Type=Erroneous initiation;
Secondary accessions: Q69YU7 Q86VZ8 Q86W97 Q9H8K3 Q9HCI9 Q9NWY0
Alternative splicing: 4 isoforms:  Q24JP5-1   Q24JP5-2   Q24JP5-3   Q24JP5-4   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for TMEM132A: NX_Q24JP5

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q24JP5

  • TMEM132A Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_060340.2  NP_821174.1  

    ENSEMBL proteins: 
     ENSP00000442754   ENSP00000405823   ENSP00000005286   ENSP00000439717   ENSP00000437848  
     ENSP00000439716  

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    Uscn Proteins for TMEM132A

    Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000139Golgi membrane IEA--
    GO:0005575cellular_component ND--
    GO:0005783endoplasmic reticulum ----
    GO:0005789endoplasmic reticulum membrane IEA--
    GO:0005794Golgi apparatus ----


    TMEM132A for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TMEM132A for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR026307 TMEM132

    Graphical View of Domain Structure for InterPro Entry Q24JP5

    ProtoNet protein and cluster: Q24JP5

    UniProtKB/Swiss-Prot: T132A_HUMAN, Q24JP5
    Similarity: Belongs to the TMEM132 family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: T132A_HUMAN, Q24JP5
    Function: May play a role in embryonic and postnatal development of the brain. Increased resistance to cell death
    induced by serum starvation in cultured cells. Regulates cAMP-induced GFAP gene expression via STAT3 phosphorylation
    (By similarity)

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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--
    GO:0005515protein binding ----


    TMEM132A for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for TMEM132A

    STRING Interaction Network Preview (showing 4 interactants - click image to see more details)

    4 Interacting proteins for TMEM132A (Q24JP53 ENSP000000052864) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HSPA5P110213, ENSP000003241734I2D: score=2 STRING: ENSP00000324173
    GSK3BP498413, ENSP000003248064I2D: score=1 STRING: ENSP00000324806
    MEGF10Q96KG73, ENSP000002744734I2D: score=2 STRING: ENSP00000274473
    UBCENSP000003448184STRING: ENSP00000344818
    About this table

    Gene Ontology (GO): 3 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007420brain development ----
    GO:0008150biological_process ND--
    GO:0043069negative regulation of programmed cell death ----


    TMEM132A for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TMEM132A

    1 HMDB Compound for TMEM132A    About this table
    CompoundSynonyms CAS #PubMed Ids
    Cyclic AMPCyclic AMP (see all 19)60-92-4--
    Search CenterWatch for drugs/clinical trials and news about TMEM132A / T132A 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TMEM132A gene (2 alternative transcripts): 
    NM_017870.3  NM_178031.2  

    Unigene Cluster for TMEM132A:

    Transmembrane protein 132A
    Hs.118552  [show with all ESTs]
    Unigene Representative Sequence: NM_017870
    15 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000537065 ENST00000544065 ENST00000453848 ENST00000005286(uc001nqi.3 uc001nqj.3)
    ENST00000543732 ENST00000540276 ENST00000537110 ENST00000534983(uc001nqk.3)
    ENST00000544098(uc001nql.1) ENST00000536409 ENST00000539899 ENST00000536928
    ENST00000538090 ENST00000540112 ENST00000535480

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    Additional cDNA sequence: 

    AK000546.1 AK023577.1 AL359557.2 BC028106.1 BC032059.1 BC114569.1 

    16 DOTS entries:

    DT.110955  DT.91825116  DT.100695860  DT.100774980  DT.100881895  DT.120716106  DT.100753315  DT.100881892 
    DT.102837369  DT.40260627  DT.101983069  DT.95132088  DT.100802774  DT.92008579  DT.92425645  DT.95191935 

    24/265 AceView cDNA sequences (see all 265):

    AW014505 AW238793 AL359557 CD675447 BU079170 AI627898 CA397294 AI582247 
    CR613429 BM126439 BM991527 BE856556 NM_178031 AW079423 BQ631785 CD671423 
    AW016515 BU078886 AI582351 BM927994 AK023577 BI754121 AI923012 AW516931 

    GeneLoc Exon Structure

    5/13 Alternative Splicing Database (ASD) splice patterns (SP) for TMEM132A (see all 13)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c ^ 3a · 3b ^ 4a · 4b · 4c · 4d ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b ^ 11 ^ 12a · 12b · 12c ^ 13a ·
    SP1:                    -     -     -     -     -     -     -                                                     -                 -     -                     
    SP2:                    -     -     -     -     -     -     -                                                     -                 -     -                     
    SP3:                                                                                                                                                            
    SP4:              -     -     -     -     -     -     -     -                                                                                                   
    SP5:                                                                                                                                                            

    ExUns: 13b · 13c ^ 14
    SP1:                  
    SP2:                  
    SP3:                  
    SP4:                  
    SP5:                  


    ECgene alternative splicing isoforms for TMEM132A

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TMEM132A expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: CCCCTTATTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See TMEM132A Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TMEM132A

    SOURCE GeneReport for Unigene cluster: Hs.118552
        SABiosciences Expression via Pathway-Focused PCR Array including TMEM132A: 
              Epithelial to Mesenchymal Transition (EMT) in human mouse rat

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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for TMEM132A gene from 4/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    lizard
    (Anolis carolinensis)
    Reptilia TMEM132A6
    --
    49(a)
    1 ↔ 1
    GL343235.1(426063-436627)
    zebrafish
    (Danio rerio)
    Actinopterygii CU929294.36
    --
    23(a)
    1 ↔ 1
    1(45441383-45452806)
    fruit fly
    (Drosophila melanogaster)
    Insecta CG144466
    --
    16(a)
    1 → many
    X(6119680-6127199)
    worm
    (Caenorhabditis elegans)
    Secernentea Y71H2AM.106
    Protein Y71H2AM.10
    15(a)
    1 → many
    III(2707729-2716269)


    ENSEMBL Gene Tree for TMEM132A (if available)
    TreeFam Gene Tree for TMEM132A (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TMEM132A gene
    TMEM132D2  TMEM132C2  TMEM132B2  TMEM132E2  
    3 SIMAP similar genes for TMEM132A using alignment to 5 protein entries:     T132A_HUMAN (see all proteins):
    TMEM132C    TMEM132B    TMEM132D

    TMEM132A for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/284 NCBI SNPs in TMEM132A are shown (see all 284    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1435338951,2
    --60689930(+) TTCTGC/TATCTG 3 -- us2k1 ut310--------
    rs1471277921,2
    --60689956(+) CTGCCA/GTTGGT 3 -- us2k1 ut310--------
    rs776617051,2
    C,F,--60690124(+) TTAAGG/ACACGC 3 -- us2k1 ut311Minor allele frequency- A:0.16WA 118
    rs10447651,2
    C,F,--60690125(+) TAAGGC/AACGCT 3 -- us2k1 ut312Minor allele frequency- A:0.06MN WA 302
    rs1414392521,2
    C,--60690348(+) GTGTC-/AAGAAAAG 3 -- us2k1 ut310--------
    rs71161751,2
    C--60690580(+) GACCAG/ATGCCC 3 -- us2k1 ut31 tfbs32Minor allele frequency- A:0.00NA 4
    rs1403184231,2
    --60690599(+) AGCCCA/GGGAGG 3 -- us2k1 ut310--------
    rs734930431,2
    C,--60690735(+) AGGTGG/TATGTC 3 -- us2k1 ut312Minor allele frequency- T:0.16WA 120
    rs1890073011,2
    --60690815(+) GAATCA/CCAGCT 3 -- us2k1 ut310--------
    rs1422124301,2
    --60690873(+) TGCACA/GCTATT 3 -- ut31 us2k10--------

    HapMap Linkage Disequilibrium report for TMEM132A (60691913 - 60704631 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for TMEM132A: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TMEM132A for disorders           About GeneDecksing

    1 disease for TMEM132A:    About MalaCards
    glioblastoma


    Export disorders for TMEM132A gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TMEM132A gene, integrated from 9 sources (see all 11):
    (articles sorted by number of sources associating them with TMEM132A)
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    world of online information

    1. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10997877)1, 2, 3 Nagase T.... Ohara O. (2000)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. Cloning and characterization of a novel GRP78-binding protein in the rat brain. (PubMed id 12514190)1, 3 Oh-hashi K....Tanaka M. (2003)
    4. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
    5. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    6. The full-ORF clone resource of the German cDNA consortium. (PubMed id 17974005)2 Bechtel S.... Schupp I. (2007)
    7. GRP78-binding protein regulates cAMP-induced glial fibrillary acidic protein expression in rat C6 glioblastoma cells. (PubMed id 16806201)1 Oh-hashi K....Kiuchi K. (2006)
    8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
    9. Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs. (PubMed id 12421765)1 Nakayama M....Ohara O. (2002)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54972 HGNC: 31092 AceView: HSPA5BP1 Ensembl:ENSG00000006118 euGenes: HUgn54972
    ECgene: TMEM132A H-InvDB: TMEM132A

    (According to HUGE)
    About This Section
    HUGE: KIAA1583

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TMEM132A Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TMEM132A gene:
    Search GeneIP for patents involving TMEM132A

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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