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Aliases for TLK1 Gene

Aliases for TLK1 Gene

  • Tousled Like Kinase 1 2 3 5
  • Tousled-Like Kinase 1 2 3 4
  • EC 2.7.11.1 4 63
  • PKU-Beta 3 4
  • Serine Threonine Protein Kinase 3
  • SNARE Protein Kinase SNAK 3
  • EC 2.7.11 63
  • KIAA0137 4

External Ids for TLK1 Gene

Previous GeneCards Identifiers for TLK1 Gene

  • GC02M169900
  • GC02M170390
  • GC02M171812
  • GC02M172051
  • GC02M171674
  • GC02M171556
  • GC02M163735

Summaries for TLK1 Gene

Entrez Gene Summary for TLK1 Gene

  • The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

GeneCards Summary for TLK1 Gene

TLK1 (Tousled Like Kinase 1) is a Protein Coding gene. Among its related pathways are ATM Pathway and p53 signaling pathway. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TLK2.

UniProtKB/Swiss-Prot for TLK1 Gene

  • Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at Ser-10.

Gene Wiki entry for TLK1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TLK1 Gene

Genomics for TLK1 Gene

Regulatory Elements for TLK1 Gene

Genomic Location for TLK1 Gene

Chromosome:
2
Start:
170,990,823 bp from pter
End:
171,231,314 bp from pter
Size:
240,492 bases
Orientation:
Minus strand

Genomic View for TLK1 Gene

Genes around TLK1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TLK1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TLK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TLK1 Gene

Proteins for TLK1 Gene

  • Protein details for TLK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UKI8-TLK1_HUMAN
    Recommended name:
    Serine/threonine-protein kinase tousled-like 1
    Protein Accession:
    Q9UKI8
    Secondary Accessions:
    • B3KR15
    • B4DX87
    • Q14150
    • Q8N591
    • Q9NYH2
    • Q9Y4F6

    Protein attributes for TLK1 Gene

    Size:
    766 amino acids
    Molecular mass:
    86700 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.
    SequenceCaution:
    • Sequence=AAF03094.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAA09486.2; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for TLK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for TLK1 Gene

Proteomics data for TLK1 Gene at MOPED

Post-translational modifications for TLK1 Gene

No Post-translational modifications

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for TLK1 (TLK1)

Domains & Families for TLK1 Gene

Gene Families for TLK1 Gene

Graphical View of Domain Structure for InterPro Entry

Q9UKI8

UniProtKB/Swiss-Prot:

TLK1_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
genes like me logo Genes that share domains with TLK1: view

Function for TLK1 Gene

Molecular function for TLK1 Gene

GENATLAS Biochemistry:
tousled-like kinase 1,nuclear serine/threonine kinase PKU beta,broadly expressed,autophosphorylating and dimerizing cell-cycle regulated displaying maximal activities during S phase,Arabidopsis thaliana homolog
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHEK1.
UniProtKB/Swiss-Prot Function:
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at Ser-10.

Enzyme Numbers (IUBMB) for TLK1 Gene

Gene Ontology (GO) - Molecular Function for TLK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA --
genes like me logo Genes that share ontologies with TLK1: view
genes like me logo Genes that share phenotypes with TLK1: view

Animal Model Products

  • Taconic Biosciences Mouse Models for TLK1

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for TLK1 Gene

Localization for TLK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for TLK1 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for TLK1 Gene COMPARTMENTS Subcellular localization image for TLK1 gene
Compartment Confidence
nucleus 5
cytosol 2

No data available for Gene Ontology (GO) - Cellular Components for TLK1 Gene

Pathways & Interactions for TLK1 Gene

genes like me logo Genes that share pathways with TLK1: view

Pathways by source for TLK1 Gene

1 BioSystems pathway for TLK1 Gene
2 Qiagen pathways for TLK1 Gene

SIGNOR curated interactions for TLK1 Gene

Activates:
Is inactivated by:
Other effect:

Gene Ontology (GO) - Biological Process for TLK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA,IDA 12660173
GO:0006886 intracellular protein transport IDA 10588641
GO:0016568 chromatin modification IEA --
genes like me logo Genes that share ontologies with TLK1: view

Drugs & Compounds for TLK1 Gene

(1) Drugs for TLK1 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Nutra 0
genes like me logo Genes that share compounds with TLK1: view

Transcripts for TLK1 Gene

Unigene Clusters for TLK1 Gene

Tousled-like kinase 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for TLK1 Gene

No ASD Table

Relevant External Links for TLK1 Gene

GeneLoc Exon Structure for
TLK1
ECgene alternative splicing isoforms for
TLK1

Expression for TLK1 Gene

mRNA expression in normal human tissues for TLK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for TLK1 Gene

This gene is overexpressed in Heart (15.9) and Peripheral blood mononuclear cells (14.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for TLK1 Gene



SOURCE GeneReport for Unigene cluster for TLK1 Gene Hs.744917

mRNA Expression by UniProt/SwissProt for TLK1 Gene

Q9UKI8-TLK1_HUMAN
Tissue specificity: Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
genes like me logo Genes that share expression patterns with TLK1: view

Protein tissue co-expression partners for TLK1 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for TLK1 Gene

Orthologs for TLK1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for TLK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia TLK1 36
  • 97 (a)
OneToOne
TLK1 35
  • 99.87 (n)
  • 100 (a)
cow
(Bos Taurus)
Mammalia TLK1 36
  • 99 (a)
OneToOne
TLK1 35
  • 95.47 (n)
  • 99.35 (a)
dog
(Canis familiaris)
Mammalia TLK1 35
  • 96.08 (n)
  • 99.48 (a)
TLK1 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Tlk1 16
Tlk1 36
  • 98 (a)
OneToOne
Tlk1 35
  • 91.91 (n)
  • 98.17 (a)
oppossum
(Monodelphis domestica)
Mammalia TLK1 36
  • 98 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia TLK1 36
  • 97 (a)
OneToOne
chicken
(Gallus gallus)
Aves TLK1 35
  • 87.44 (n)
  • 96.52 (a)
TLK1 36
  • 96 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia TLK1 36
  • 96 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.22395 35
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.902 35
tlk1 35
  • 82.12 (n)
  • 94.41 (a)
zebrafish
(Danio rerio)
Actinopterygii tlk1a 36
  • 85 (a)
OneToMany
tlk1b 36
  • 85 (a)
OneToMany
tlk1a 35
  • 74.52 (n)
  • 87.66 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000043 35
  • 56.27 (n)
  • 62.94 (a)
fruit fly
(Drosophila melanogaster)
Insecta BcDNA:GH07910 37
  • 72 (a)
tlk 36
  • 27 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea tlk-1 36
  • 34 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MPS1 36
  • 15 (a)
OneToMany
barley
(Hordeum vulgare)
Liliopsida Hv.12931 35
corn
(Zea mays)
Liliopsida Zm.13254 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.566 36
  • 64 (a)
OneToMany
Species with no ortholog for TLK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for TLK1 Gene

ENSEMBL:
Gene Tree for TLK1 (if available)
TreeFam:
Gene Tree for TLK1 (if available)

Paralogs for TLK1 Gene

Paralogs for TLK1 Gene

(4) SIMAP similar genes for TLK1 Gene using alignment to 6 proteins:

Pseudogenes.org Pseudogenes for TLK1 Gene

genes like me logo Genes that share paralogs with TLK1: view

Variants for TLK1 Gene

Sequence variations from dbSNP and Humsavar for TLK1 Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type
VAR_041215 -
rs218310 -- 171,201,163(+) ACAGA(C/T)GTGAG intron-variant
rs728456 -- 171,178,228(-) CCTGA(A/G)TTTTA intron-variant
rs728457 -- 171,178,174(-) GTTTA(C/T)TGACA intron-variant
rs1019711 -- 171,182,024(+) AACCA(C/G)TGGCT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for TLK1 Gene

Variant ID Type Subtype PubMed ID
nsv834456 CNV Loss 17160897
nsv3030 CNV Insertion 18451855
esv2664440 CNV Deletion 23128226
nsv522291 CNV Loss 19592680
esv2660349 CNV Deletion 23128226

Variation tolerance for TLK1 Gene

Residual Variation Intolerance Score: 12.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.86; 34.86% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for TLK1 Gene

HapMap Linkage Disequilibrium report
TLK1
Human Gene Mutation Database (HGMD)
TLK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TLK1 Gene

Disorders for TLK1 Gene

Relevant External Links for TLK1

Genetic Association Database (GAD)
TLK1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
TLK1

No disorders were found for TLK1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for TLK1 Gene

Publications for TLK1 Gene

  1. Human tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint. (PMID: 12660173) Groth A. … Hansen K. (EMBO J. 2003) 2 3 4 67
  2. PKU-beta/TLK1 regulates myosin II activities, and is required for accurate equaled chromosome segregation. (PMID: 18838128) Hashimoto M. … Date T. (Mutat. Res. 2008) 3 23
  3. Suppression of tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1. (PMID: 12955071) Krause D.R. … Khanna K. (Oncogene 2003) 3 23
  4. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases. (PMID: 11470414) Sillje H.H.W. … Nigg E.A. (Curr. Biol. 2001) 3 23
  5. cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal. (PMID: 9427565) Yamakawa A. … Date T. (Gene 1997) 2 3

Products for TLK1 Gene

Sources for TLK1 Gene

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