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TIGD5 Gene

protein-coding GIFtS: 42
GCID: GC08P144680

Tigger Transposable Element Derived 5

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
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Aliases
Tigger Transposable Element Derived 5¹ ²
Tigger Transposable Element-Derived Protein 5²

External Ids:	HGNC: 18336¹	Entrez Gene: 84948²	Ensembl: ENSG00000179886⁷	UniProtKB: Q53EQ6³

Export aliases for TIGD5 gene to outside databases

Previous GC identifers: GC08P142725 GC08P144908 GC08P144454 GC08P144785 GC08P144751 GC08P139914

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for TIGD5 Gene:

The protein encoded by this gene belongs to the tigger subfamily of the pogo superfamily of DNA-mediated

transposons in humans. These proteins are related to DNA transposons found in fungi and nematodes, and more

distantly to the Tc1 and mariner transposases. They are also very similar to the major mammalian centromere

protein B. The exact function of this gene is not known. (provided by RefSeq, Jul 2008)

GeneCards Summary for TIGD5 Gene:

TIGD5 (tigger transposable element derived 5) is a protein-coding gene. An important paralog of this gene is TIGD2.

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:

NC_000008.10 NC_018919.2 NT_008046.17 NT_187571.1

Regulatory elements:

Regulatory transcription factor binding sites in the TIGD5 gene promoter:
GCNF AP-4 YY1 CREB E2F-1 E2F GCNF-1 STAT3 ZIC2/Zic2 GCNF-2
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: TIGD5 promoter sequence

Search Chromatin IP Primers for TIGD5

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TIGD5

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8q24.3 Ensembl cytogenetic band: 8q24.3 HGNC cytogenetic band: 8q24.3

TIGD5 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TIGD5 gene location

GeneLoc information about chromosome 8 GeneLoc Exon Structure

GeneLoc location for GC08P144680: view genomic region (about GC identifiers)

Start:	144,680,074 bp from pter	End:	144,682,485 bp from pter
Size:	2,412 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: TIGD5_HUMAN, Q53EQ6 (See protein sequence)

Recommended Name: Tigger transposable element-derived protein 5

Size: 642 amino acids; 69222 Da

Sequence caution: Sequence=AAH32632.1; Type=Erroneous initiation; Note=Translation N-terminally extended;

Sequence=BAB55398.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAD97303.1;

Type=Erroneous initiation; Note=Translation N-terminally extended;

Secondary accessions: E7EWS2 Q6NT83 Q8N5A1 Q96JW8

Alternative splicing: 2 isoforms: Q53EQ6-1 Q53EQ6-2

Explore the universe of human proteins at neXtProt for TIGD5: NX_Q53EQ6

Explore proteomics data for TIGD5 at MOPED

Post-translational modifications:

Modification sites at neXtProt

Modification sites at PhosphoSitePlus

See TIGD5 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_116251.4

ENSEMBL proteins:

ENSP00000421489 ENSP00000315906

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro protein domains:

IPR006600 HTH_CenpB_DNA-bd_dom

IPR004875 DDE_SF_endonuclease_CENPB-like

IPR009057 Homeodomain-like

IPR007889 HTH_Psq

Graphical View of Domain Structure for InterPro Entry Q53EQ6

ProtoNet protein and cluster: Q53EQ6

1 Blocks protein domain: IPB004875 CENP-B protein

UniProtKB/Swiss-Prot: TIGD5_HUMAN, Q53EQ6

Similarity: Belongs to the tigger transposable element derived protein family

Similarity: Contains 2 DDE domains

Similarity: Contains 1 HTH CENPB-type DNA-binding domain

Similarity: Contains 1 HTH psq-type DNA-binding domain

Find genes that share domains with TIGD5 About GenesLikeMe

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Gene Ontology (GO): 3 molecular function terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--
GO:0003676	nucleic acid binding	--	--
GO:0003677	DNA binding	IEA	--

Find genes that share ontologies with TIGD5 About GenesLikeMe

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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot

TIGD5_HUMAN, Q53EQ6: Nucleus (Probable)

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
nucleus	4
cytosol	2
extracellular	1
mitochondrion	1

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Gene Ontology (GO): 2 cellular component terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005575	cellular_component	ND	--
GO:0005634	nucleus	IEA	--

Find genes that share ontologies with TIGD5 About GenesLikeMe

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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Custom Pathway & Disease-focused RT² Profiler PCR Arrays for TIGD5
Interactions:

Search GeneGlobe Interaction Network for TIGD5

STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

1 Interacting protein for TIGD5 (ENSP00000315906⁴) via UniProtKB, MINT, STRING, and/or I2D

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
UBC	ENSP00000344818⁴	STRING: ENSP00000344818

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Gene Ontology (GO): 1 biological process term: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008150	biological_process	ND	--

Find genes that share ontologies with TIGD5 About GenesLikeMe

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore

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(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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REFSEQ mRNAs for TIGD5 gene:

NM_032862.4

Unigene Cluster for TIGD5:

Tigger transposable element derived 5

Hs.71574 [show with all ESTs]

Unigene Representative Sequence: NM_032862

2 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000504548 ENST00000321385(uc003yyx.2)

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Additional mRNA sequence:

AK027832.1 AK223583.1 BC032632.1 BC069229.1

1 DOTS entry:

DT.453841

Selected AceView cDNA sequences (see all 43):

BM727243 NM_032862 AI648562 AI133279 BE350277 CB055230 CB055231 BC069229

BM049601 BM915841 AW665438 BU740809 BI857753 BV202302 BX113218 AI733896

AI376573 BM471048 BM556133 BC032632 AW151443 AK027832 AI991797 AA622968

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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TIGD5 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: CACTGGACGA

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TIGD5 Protein expression data from MOPED¹, PaxDb² and MaxQB³:

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TIGD5 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.71574

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In Situ
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(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of chordates.

Orthologs for TIGD5 gene from Selected species (see all 9) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Tigd5^{1 , 5}	tigger transposable element derived 5^{1, 5}	81.53(n)¹ 85.93(a)¹		15 (35.07 cM)⁵ 105734¹ NM_178646.4¹ NP_848761.2¹ 75909760⁵
chicken (Gallus gallus)	Aves	TIGD5¹	tigger transposable element derived 5	68.5(n) 73.95(a)		101749031 XM_004940092.1 XP_004940149.1
lizard (Anolis carolinensis)	Reptilia	--	Uncharacterized protein	21(a)	many ↔ many	2(103059200-103060456)
tropical clawed frog (Xenopus tropicalis)	Amphibia	tigd5¹	tigger transposable element derived 5	64.48(n) 65.67(a)		100491467 XM_004915012.1 XP_004915069.1

ENSEMBL Gene Tree for TIGD5 (if available)
TreeFam Gene Tree for TIGD5 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
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Paralogs for TIGD5 gene

TIGD2² JRKL² TIGD1² TIGD7² CENPB² TIGD6² TIGD3² TIGD4²

1 SIMAP similar gene for TIGD5 using alignment to 1 protein entry: TIGD5_HUMAN:

JH8

Find genes that share paralogs with TIGD5 About GenesLikeMe

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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SNP ID	Valid	Clinical significance	Chr 8 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs146036086^1,2	C	--	144682235(+)	`TCAGGA/GCAGGC`	1	--	ut3¹		0	--	--	--	--
rs3210186^1,2	C,F,A,H	--	144682311(+)	`CCGTGG/CGGCTG`	1	--	ut3¹		10		NS EA NA	660
rs139786888^1,2		--	144682351(+)	`CTGGAC/TGAGGG`	1	--	ut3¹		0	--	--	--	--
rs143174212^1,2	C	--	144682358(+)	`AGGGCC/TGAAAC`	1	--	ut3¹		0	--	--	--	--
rs112637095^1,2	C,F	--	144682428(+)	`AGTGGC/TGAAGC`	1	--	ut3¹		2		NA	122
rs146194616^1,2		--	144682451(+)	`TCTATG/TTTTTT`	1	--	ut3¹		0	--	--	--	--
rs148428106^1,2	C	--	144682701(+)	`TATTCC/TGTGGG`	1	--	ds500¹		0	--	--	--	--
rs6558375^1,2	C,F,A	--	144682748(+)	`GCCAAC/TGGCAG`	1	--	ds500¹		12		NA WA CSA EA	375
rs184064762^1,2		--	144682877(+)	`CCACCC/TTCTGT`	1	--	ds500¹		0	--	--	--	--
rs10282929^1,2,4		--	--	see VAR_030043	2		mis⁴		0	--	--	--	--

HapMap Linkage Disequilibrium report for TIGD5 (144680074 - 144682485 bp)

Structural Variations
Database of Genomic Variants (DGV) Selected variations for TIGD5 (see all 17): About this table

Variant ID	Type	Subtype	PubMed ID
esv26007	CNV	Loss	19812545
nsv466004	CNV	Loss	19166990
dgv7932n71	CNV	Loss	21882294
nsv516210	CNV	Loss	19592680
nsv891739	CNV	Loss	21882294
dgv7936n71	CNV	Loss	21882294
nsv891734	CNV	Loss	21882294
dgv7928n71	CNV	Loss	21882294
dgv7934n71	CNV	Loss	21882294
dgv7933n71	CNV	Loss	21882294

Site Specific Mutation Identification with PCR Assays

SeqTarget long-range PCR primers for resequencing TIGD5

DNA2.0 Custom Variant and Variant Library Synthesis for TIGD5

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for TIGD5 gene integrated from 10 sources:
(articles sorted by number of sources associating them with TIGD5)

Utopia: connect your pdf to the dynamic
world of online information

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S.... Malek J. (Genome Res. 2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (Nat. Genet. 2004)
The proteomic analysis of endogenous FAT10 substrates identifies p62/SQSTM1 as a substrate of FAT10ylation. (PubMed id 22797925)¹ Aichem A....Groettrup M. (J. Cell. Sci. 2012)
Systematic and quantitative assessment of the ubiquitin-modified proteome. (PubMed id 21906983)¹ Kim W....Gygi S.P. (Mol. Cell 2011)
Global identification of modular cullin-RING ligase substrates. (PubMed id 21963094)¹ Emanuele M.J....Elledge S.J. (Cell 2011)
DNA sequence and analysis of human chromosome 8. (PubMed id 16421571)² Nusbaum C....Lander E.S. (Nature 2006)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 84948	HGNC: 18336	AceView: TIGD5	Ensembl:ENSG00000179886	euGenes: HUgn84948
ECgene: TIGD5	H-InvDB: TIGD5

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for TIGD5	Pharmacogenomics, SNPs, Pathways

Intellectual Property for TIGD5 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for TIGD5 gene:
Search GeneIP for patents involving TIGD5

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from genOway)
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	Browse OriGene Antibodies		OriGene RNAi products in human, mouse, rat for TIGD5
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	OriGene Custom Mass Spec		OriGene clones in human, mouse for TIGD5
	OriGene qSTAR qPCR primer pairs in human, mouse for TIGD5		Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
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	Block miRNA regulation of human, mouse, rat TIGD5 using miScript Target Protectors		SeqTarget long-range PCR primers for resequencing TIGD5
	DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat TIGD5		Predesigned siRNA for gene silencing in human, mouse, rat TIGD5
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	Custom PCR Arrays for TIGD5		Search Chromatin IP Primers for TIGD5
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	GenScript Custom Purified and Recombinant Proteins Services for TIGD5		GenScript cDNA clones with any tag delivered in your preferred vector for TIGD5
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	Vector-based siRNA and miRNA, Ready for Transfection		Gene Mutant Library, Variants up to 10^11
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GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 2 Nov 2014 , 8 Jan 2015

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Hot genes Disease genes TIGD5 gene at Home site.

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TIGD5 Gene

Tigger Transposable Element Derived 5

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TIGD5 Gene

Tigger Transposable Element Derived 5

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