THEM5 Gene
protein-coding GIFtS: 43
GCID: GC01M151818
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|
thioesterase superfamily member 5
| |
Aliases
for THEM5 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Thioesterase Superfamily Member 51 2 3 | | Acyl-Coenzyme A Thioesterase THEM52 | | Acyl-CoA Thioesterase THEM52 3 | | ACOT153 | | Acyl-Coenzyme A Thioesterase 152 3 | | EC 3.1.2.23 | | FLJ379641 | | |
Export aliases for THEM5 gene to outside databasesPrevious GC identifers: GC01M148632 GC01M150084 GC01M123196 |
Summaries
for THEM5 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: ACO15_HUMAN, Q8N1Q8Function: Has acyl-CoA thioesterase activity towards long-chain (C16 and C18) fatty acyl-CoA substrates, with apreference for linoleyl-CoA and other unsaturated long-chain fatty acid-CoA esters. Plays an important role inmitochondrial fatty acid metabolism, and in remodeling of the mitochondrial lipid cardiolipin. Required for normalmitochondrial function
|
Genomic Views
for THEM5 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000001.10 NC_018912.1 NT_004487.19
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the THEM5 gene promoter:
c-Fos AP-1 Fra-1 FosB Evi-1 C/EBPalpha FOXO4 c-Jun
Other transcription factors
Search SABiosciences Chromatin IP Primers for THEM5
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat THEM5 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 1q21.3 Ensembl cytogenetic band: 1q21.3 HGNC cytogenetic band: 1q21.3THEM5 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 1 GeneLoc Exon Structure GeneLoc location for GC01M151818: view genomic region
(about GC identifiers)
Start:
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151,818,220 bp from pter |
End:
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151,826,173 bp from pter |
Size:
|
7,954 bases |
Orientation:
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minus strand |
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Proteins
for THEM5 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: ACO15_HUMAN, Q8N1Q8 (See
protein sequence)Recommended Name: Acyl-coenzyme A thioesterase THEM5 Size: 247 amino acids; 27677 Da
Subunit: Homodimer
Subcellular location: Mitochondrion matrix
1 PDB 3D structure from  and Proteopedia  for THEM5:4AE7 (3D)
Secondary accessions: Q5T1C3Explore the universe of human proteins at neXtProt for THEM5: NX_Q8N1Q8
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8N1Q8
THEM5 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_872384.1
ENSEMBL proteins: ENSP00000357807 ENSP00000406809
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005739 | mitochondrion |
-- | -- | | GO:0005759 | mitochondrial matrix |
IDA | -- |
THEM5 for ontologies About GeneDecksing
THEM5 Antibody Products:
Assay Products for THEM5: |
Protein
Domains /
Families
for THEM5 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
THEM5 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q8N1Q8ProtoNet protein and cluster: Q8N1Q8 UniProtKB/Swiss-Prot: ACO15_HUMAN, Q8N1Q8Similarity: Belongs to the THEM4/THEM5 thioesterase family |
Function
for THEM5 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: ACO15_HUMAN, Q8N1Q8Function: Has acyl-CoA thioesterase activity towards long-chain (C16 and C18) fatty acyl-CoA substrates, with apreference for linoleyl-CoA and other unsaturated long-chain fatty acid-CoA esters. Plays an important role inmitochondrial fatty acid metabolism, and in remodeling of the mitochondrial lipid cardiolipin. Required for normalmitochondrial functionCatalytic activity: Palmitoyl-CoA + H(2)O = CoA + palmitateBiophysicochemical properties: Kinetic parameters: KM=2.4 uM for palmitoyl-CoA (C16:0); KM=4.2 uM for stearoyl-CoA(C18:0); KM=2.9 uM for oleoyl-CoA (C18:1); KM=4.2 uM for linoleyl-CoA (C18:2);Enzyme Number (IUBMB): EC 3.1.2.21
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for THEM5 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for THEM5
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: THEM5 (NM_182578) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for THEM5 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat THEM5 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for THEM5 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016290 | palmitoyl-CoA hydrolase activity |
IDA | -- | | GO:0016787 | hydrolase activity |
-- | -- |
THEM5 for ontologies About GeneDecksing
Animal Models:
Mouse knock-out Them5tm1Hem for THEM5
3 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Them5):
THEM5 for phenotypes About GeneDecksing
|
Pathways & Interactions
for THEM5 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for THEM5
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
2 Interacting proteins for THEM5 (ENSP000003578074) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 3 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006631 | fatty acid metabolic process |
IEA | -- | | GO:0035336 | long-chain fatty-acyl-CoA metabolic process |
IDA | -- | | GO:0035965 | cardiolipin acyl-chain remodeling |
ISS | -- |
THEM5 for ontologies About GeneDecksing
|
Drugs & Compounds
for THEM5 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for THEM5
Search CenterWatch for drugs/clinical trials and news about THEM5 / ACO15
|
Transcripts
for THEM5 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for THEM5 gene: NM_182578.3 Unigene Cluster for THEM5: Thioesterase superfamily member 5 Hs.132648 [show with all ESTs]Unigene Representative Sequence: AK0952832 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000368817(uc021oyw.1) ENST00000453881
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for THEM5 (see all 3)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for THEM5
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: THEM5 (NM_182578) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for THEM5 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat THEM5 |
Additional cDNA sequence: AK095283.1 BC101610.1 BC112239.1 3 DOTS entries: DT.100009058 DT.100723753 DT.40129428 3 AceView cDNA sequences: CD103453 AI032980 BX093845
GeneLoc Exon Structure
|
Expression
for THEM5 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| THEM5 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TGGCAAGTGC
About this image
See THEM5 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for THEM5
SOURCE GeneReport for Unigene cluster: Hs.132648
SABiosciences Custom PCR Arrays for THEM5
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for THEM5
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat THEM5 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat THEM5 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat THEM5 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for THEM5 |
Orthologs
for THEM5 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for THEM5 gene from 2/11 species (see all 11) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
lizard
(Anolis carolinensis) |
Reptilia |
THEM56 |
-- |
30(a) |
1 → many |
GL344049.1(19245-22505) |
zebrafish
(Danio rerio) |
Actinopterygii |
zgc:1588476 |
zgc:158847 |
29(a) |
1 → many |
16(19826911-19861475) |
ENSEMBL Gene Tree for THEM5 (if available)
TreeFam Gene Tree for THEM5 (if available) |
Paralogs
for THEM5 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for THEM5 gene
- THEM42
1 SIMAP similar gene for THEM5 using alignment to 2 protein entries: ACO15_HUMAN (see all proteins):THEM4
THEM5 for paralogs About GeneDecksing
|
Genomic Variants
for THEM5 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 1 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for THEM5 (151818220 - 151826173 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for THEM5: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing THEM5 |
|
Disorders
/ Diseases
for THEM5 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
THEM5 for disorders About GeneDecksing
5  diseases for THEM5: About MalaCardsmedian rhomboid glossitis benign migratory glossitis glossitis hepatitis b
hepatitis
1 disease from the University of Copenhagen DISEASES database for THEM5:Median rhomboid glossitis Human Genome Epidemiology (HuGE) Navigator: THEM5 (3 documents)
Export disorders for THEM5 gene to outside databases
|
Publications
for THEM5 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for THEM5 gene integrated from 9 sources:
(articles sorted by number of sources associating them with THEM5) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development. (PubMed id 22586271)1, 2 Zhuravleva E.... Hemmings B.A. (2012)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- New genetic associations detected in a host response study to hepatitis B vaccine. (PubMed id 20237496)1 Davila S....Seielstad M. (2010)
- Genetic variants in nuclear-encoded mitochondrial gen es influence AIDS progression. (PubMed id 20877624)1 Hendrickson S.L....O'Brien S.J. (2010)
- The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)2 Gregory S.G.... Bentley D.R. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S....Malek J. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for THEM5 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing THEM5 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing THEM5 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing THEM5 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for THEM5 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for THEM5 gene:
Search GeneIP for patents involving THEM5
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for THEM5 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for THEM5 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for THEM5 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for THEM5 | | | OriGene Protein Over-expression Lysate for THEM5 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for THEM5 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for THEM5 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for THEM5 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for THEM5 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for THEM5 | | OriGene Custom Protein Services for THEM5 | | | OriGene Custom Immunoassay Development | | |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat THEM5 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing THEM5 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat THEM5 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat THEM5 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat THEM5 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat THEM5 |
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 |  |  |  | |
| | | | Search Tocris compounds for THEM5 |
| |  |  |  |  | | | | |
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 | | | THEM5 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for THEM5 |
|  |  |  | | | | Search ThermoFisher Antibodies for THEM5 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat THEM5 |
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