TFB2M Gene
protein-coding GIFtS: 52
GCID: GC01M246703
|
|
transcription factor B2, mitochondrial
| |
Aliases for TFB2M gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb) About This Section
|
| Aliases |
|---|
| Transcription Factor B2, Mitochondrial1 2 | | HCV NS5A-Transactivated Protein 52 3 | | Hkp11 2 | | MtTFB21 | | Hepatitis C Virus NS5A-Transactivated Protein 52 3 | | S-Adenosylmethionine-6-N', N'-Adenosyl(RRNA) Dimethyltransferase 22 3 | | Mitochondrial 12S RRNA Dimethylase 22 3 | | FLJ226611 | | Mitochondrial Transcription Factor B22 3 | | FLJ231821 | | H-MtTFB1 | | Dimethyladenosine Transferase 2, Mitochondrial2 | | H-MtTFB21 | | EC 2.1.1.-3 | | HTFB2M1 | | NS5ATP53 |
Export aliases for TFB2M gene to outside databasesPrevious GC identifers: GC01M244607 GC01M241980 GC01M242800 GC01M243651 GC01M243652 GC01M243029 GC01M244770 GC01M217089 |
Summaries for TFB2M gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| UniProtKB/Swiss-Prot: TFB2M_HUMAN, Q9H5Q4Function: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA atthe conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction withPOLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, itactivates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity Gene Wiki entry for TFB2M
|
Genomic Views for TFB2M gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics) About This Section
| RefSeq DNA sequence:- NC_000001.10 NC_018912.1 NT_167186.1
Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the TFB2M gene promoter: Max1 E2F-3a E2F-4 E2F-5 Sp1 E2F-2 E2F E2F-1 c-Myc Other transcription factors
Search SABiosciences Chromatin IP Primers for TFB2M
Epigenetics:
|  | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TFB2M |
Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 1q44 Ensembl cytogenetic band: 1q44 HGNC cytogenetic band: 1q44TFB2M Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)

GeneLoc information about chromosome 1 GeneLoc Exon Structure GeneLoc location for GC01M246703: view genomic region
(about GC identifiers)
Start:
|
246,703,862 bp from pter |
End:
|
246,729,626 bp from pter |
Size:
|
25,765 bases |
Orientation:
|
minus strand |
|
Proteins for TFB2M gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
| UniProtKB/Swiss-Prot: TFB2M_HUMAN, Q9H5Q4 (See
protein sequence)Recommended Name: Dimethyladenosine transferase 2, mitochondrial precursor Size: 396 amino acids; 45349 Da
Subunit: Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM
Subcellular location: Mitochondrion
Sequence caution: Sequence=BAB15441.1; Type=Erroneous initiation;
Secondary accessions: Q9H626Explore the universe of human proteins at neXtProt for TFB2M: NX_Q9H5Q4
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9H5Q4 TFB2M Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_071761.1 ENSEMBL proteins: ENSP00000355471 ENSP00000442426 Reactome Protein details: Q9H5Q4 Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
TFB2M for ontologies About GeneDecksing
TFB2M Antibody Products: Assay Products for TFB2M: |
Protein
Domains / Families for TFB2M gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TFB2M for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9H5Q4ProtoNet protein and cluster: Q9H5Q4 UniProtKB/Swiss-Prot: TFB2M_HUMAN, Q9H5Q4Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily |
Function for TFB2M gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
| Function Summary: UniProtKB/Swiss-Prot: TFB2M_HUMAN, Q9H5Q4Function: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA atthe conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction withPOLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, itactivates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activityInduction: By the nuclear respiratory factors NRF1 and NRF2/GABPB2 and PGC-1 coactivatorsEnzyme Number (IUBMB): EC 2.1.1.-1
Clone Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TFB2M (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TFB2M OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
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In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TFB2M |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0000179 | rRNA (adenine-N6,N6-)-dimethyltransferase activity |
IEA | -- | | GO:0003712 | transcription cofactor activity |
IDA | 12068295 |
TFB2M for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for TFB2M:
|
Pathways & Interactions for TFB2M gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
| Unified GeneCards pathways - 5/6 super-pathways (see all 6) About this table  See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Transcription from mitochondrial promoters | | | 2 | Transcription | | | 3 | Mitochondrial Gene Expression | | | 4 | RNA Polymerase III Transcription Initiation | | | 5 | Generic Transcription Pathway | |
Pathway sources See GeneCards unified pathways Show all pathways
2 BioSystems Pathways for TFB2M 
5
Reactome Pathways for TFB2M
TFB2M for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for TFB2M
STRING Interaction
Network Preview (showing 5 interactants - click image to see more details)
 5/8 Interacting proteins for TFB2M (Q9H5Q43 ENSP000003554714) via UniProtKB, MINT, STRING, and/or I2D (see all 8)About this table
Gene Ontology (GO): 4 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006390 | transcription from mitochondrial promoter |
TAS | -- | | GO:0006391 | transcription initiation from mitochondrial promoter |
TAS | -- | | GO:0010467 | gene expression |
TAS | -- | | GO:0045893 | positive regulation of transcription, DNA-dependent |
IDA | -- |
TFB2M for ontologies About GeneDecksing
|
Drugs & Compounds for TFB2M gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
Browse Tocris compounds for TFB2M
1 HMDB Compound for TFB2M About this table Search CenterWatch for drugs/clinical trials and news about TFB2M 
|
Transcripts for TFB2M gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
) About This Section
| REFSEQ mRNAs for TFB2M gene: NM_022366.2 Unigene Cluster for TFB2M: Transcription factor B2, mitochondrial Hs.7395 [show with all ESTs]Unigene Representative Sequence: AK0268352 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000366514(uc001ibn.3 uc010pys.1) ENST00000544618
Clone Products: |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TFB2M (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TFB2M OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: TFB2M (NM_022366) | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TFB2M | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TFB2M  |
Additional cDNA sequence: AF529366.1 AK026314.1 AK026835.1 BC003383.1 BC042010.1 6 DOTS entries: DT.95376378 DT.444130 DT.95330095 DT.92432619 DT.100773915 DT.95154287 24/156 AceView cDNA sequences (see all 156): AI093305 T36250 AI478439 AI379324 AL698965 BU631879 BM757047 BM747919 BG940165 CK725134 AA720622 BE302270 BU683428 AA643133 AA242868 AA568574 AA460334 BM678104 AA757357 AI273642 BU733948 BM725623 AW051829 CB243880 GeneLoc Exon Structure
3 Alternative Splicing Database (ASD) splice patterns (SP) for TFB2M About this scheme
| ExUns: | 1a | · | 1b | ^ | 2a | · | 2b | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | |
| SP1: | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | - |   | - |   | - |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
ECgene alternative splicing isoforms for TFB2M
|
Expression for TFB2M gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TFB2M expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this imageBioGPS CGAP TAG: --
About this image See TFB2M Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for TFB2M
SOURCE GeneReport for Unigene cluster: Hs.7395 UniProtKB/Swiss-Prot: TFB2M_HUMAN, Q9H5Q4Tissue specificity: Ubiquitously expressed SABiosciences Custom PCR Arrays for TFB2M
Primer Products: |  | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TFB2M Browse OriGene validated miRNA SYBR primer pairs
| |  | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat TFB2M | |  | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TFB2M | |  | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TFB2M | In Situ Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TFB2M |
Orthologs for TFB2M gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for TFB2M gene from 5/14 species (see all 14) About this table
| Organism |
Taxonomic classification |
Gene |
Description |
Human Similarity |
Orthology Type |
Details |
mouse (Mus musculus) |
Mammalia |
Tfb2m1 , 5 |
transcription factor B2, mitochondrial1, 5 |
69.49(n)1 53.33(a)1 |
  |
1 (83.56 cM)5 152781 NM_008249.41 NP_032275.21 1795280555 |
chicken (Gallus gallus) |
Aves |
TFB2M1 |
transcription factor B2, mitochondrial |
58.6(n) 43.95(a) |
  |
421488 XM_419535.3 XP_419535.2 |
lizard (Anolis carolinensis) |
Reptilia |
TFB2M6 |
-- |
45(a) |
1 ↔ 1 |
GL343534.1(39542-48989) |
tropical clawed frog (Xenopus tropicalis) |
Amphibia |
Str.167042 |
Transcribed sequence with weak similarity to protein more |
77.06(n) |
  |
CF374635.1 |
zebrafish (Danio rerio) |
Actinopterygii |
tfb2m1 |
transcription factor B2, mitochondrial |
51.97(n) 40.37(a) |
  |
569468 NM_001113617.1 NP_001107089.1 |
ENSEMBL Gene Tree for TFB2M (if available) TreeFam Gene Tree for TFB2M (if available)  |
Paralogs for TFB2M gene(Paralogs according to
1HomoloGene, 2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68) About This Section
| Paralogs for TFB2M gene
TFB2M for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for TFB2M PGOHUM00000243046
|
Genomic Variants for TFB2M gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical significance | Chr 1 pos | Sequence | # | AA Chg | Type | More | # | Allele freq | Pop | Total sample | More |
|---|
HapMap Linkage Disequilibrium report for TFB2M (246703862 - 246729626 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 1 variation for TFB2M 1 CNV: 3338  | SABiosciences Cancer Mutation PCR Assays |
|  | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TFB2M |
|
Disorders
/ Diseases for TFB2M gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TFB2M for disorders About GeneDecksing
OMIM gene information: 607055
OMIM disorders: --
3 diseases for TFB2M: About MalaCardshepatitis c hepatitis parkinson's disease Human Genome Epidemiology (HuGE) Navigator: TFB2M (4 documents) Export disorders for TFB2M gene to outside databases
|
Publications for TFB2M gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TFB2M gene, integrated from 9 sources (see all 27): (articles sorted by number of sources associating them with TFB2M) | |  | Utopia: connect your pdf to the dynamic world of online information |
- The mitochondrial RNA polymerase contributes critically to promoter specificity in mammalian cells. (PubMed id 15526033)1, 2, 9 Gaspari M.... Gustafsson C.M. (2004)
- Human mitochondrial transcription revisited: only TFA M and TFB2M are required for transcription of the mitochondrial genes in vitro. (PubMed id 20410300)1, 2 Litonin D....Temiakov D. (2010)
- Evidence for an early gene duplication event in the evolution of the mitochondrial transcription factor B family and maintenance of rRNA methyltransferase activity in human mtTFB1 and mtTFB2. (PubMed id 17031457)1, 2 Cotney J. and Shadel G.S. (2006)
- The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
- Control of mitochondrial transcription specificity factors (TFB1M and TFB2M) by nuclear respiratory factors (NRF-1 and NRF-2) and PGC-1 family coactivators. (PubMed id 15684387)1, 2 Gleyzer N.... Scarpulla R.C. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Human mitochondrial transcription factor B1 interacts with the C- terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity. (PubMed id 12897151)1, 2 McCulloch V. and Shadel G.S. (2003)
- Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA. (PubMed id 12068295)1, 2 Falkenberg M....Gustafsson C.M. (2002)
- Functional dissection of lysine deacetylases reveals t hat HDAC1 and p300 regulate AMPK. (PubMed id 22318606)1 Lin Y.Y....Boeke J.D. (2012)
|
External Searches for TFB2M gene
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing TFB2M gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TFB2M gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TFB2M gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| Name | Description |
| PharmGKB entry for TFB2M | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for TFB2M gene: Search GeneIP for patents involving TFB2M
GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products for TFB2M gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
|
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 | |
 |
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| | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TFB2M |
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