TCFL5 Gene
protein-coding GIFtS: 48
GCID: GC20M061472
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transcription factor-like 5 (basic helix-loop-helix)
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Aliases
for TCFL5 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Transcription Factor-Like 5 (Basic Helix-Loop-Helix)1 2 | | Cha Transcription Factor2 3 | | CHA1 2 3 | | HPV-16 E2-Binding Protein 12 3 | | E2BP-11 2 3 | | HPV-16 E2 Binding Protein 12 | | Figlb1 2 | | Transcription Factor-Like 5 Protein2 | | BHLHe821 | | E2BP13 |
Export aliases for TCFL5 gene to outside databasesPrevious GC identifers: GC20M061349 GC20M061309 GC20M062151 GC20M062198 GC20M060942 GC20M058190 |
Summaries
for TCFL5 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
UniProtKB/Swiss-Prot: TCFL5_HUMAN, Q9UL49Function: Putative transcription factor. Isoform 3 may play a role in early spermatogenesis
|
Genomic Views
for TCFL5 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_018931.1 NC_000020.10 NT_011333.6
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the TCFL5 gene promoter:
ER-alpha AML1a POU3F1 CREB POU2F1 deltaCREB POU2F1a FOXJ2 (long isoform) SEF-1 (1) FOXJ2
Other transcription factors
Search SABiosciences Chromatin IP Primers for TCFL5
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TCFL5 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 20q13.3-qter Ensembl cytogenetic band: 20q13.33 HGNC cytogenetic band: 20q13.3-qterTCFL5 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 20 GeneLoc Exon Structure GeneLoc location for GC20M061472: view genomic region
(about GC identifiers)
Start:
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61,472,467 bp from pter |
End:
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61,493,115 bp from pter |
Size:
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20,649 bases |
Orientation:
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minus strand |
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Proteins
for TCFL5 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: TCFL5_HUMAN, Q9UL49 (See
protein sequence)Recommended Name: Transcription factor-like 5 protein Size: 500 amino acids; 52697 Da
Subunit: Efficient DNA binding requires dimerization with another bHLH protein
Subcellular location: Nucleus
Developmental stage: Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not thoseat leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartmentof the seminiferous tubule or spermatids
Miscellaneous: Antibodies against TCFL5 are present in sera from patients with Chagas' disease, an autoimmune diseasecaused by Trypanosoma cruzi. Two different epitopes that mimic Trypanosoma cruzi antigens have been identified: R1 andR3 epitopes, which are recognized by T- and B-cells, respectively
Sequence caution: Sequence=BAA36557.1; Type=Erroneous initiation;
Secondary accessions: O94771 Q9BYW0Alternative splicing: 3 isoforms: Q9UL49-3 Q9UL49-1 Q9UL49-2 Explore the universe of human proteins at neXtProt for TCFL5: NX_Q9UL49
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9UL49
TCFL5 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_006593.2
ENSEMBL proteins: ENSP00000334294 ENSP00000217162
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
TCFL5 for ontologies About GeneDecksing
TCFL5 Antibody Products:
Assay Products for TCFL5: |
Protein
Domains /
Families
for TCFL5 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
TCFL5 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry Q9UL49ProtoNet protein and cluster: Q9UL49 1 Blocks protein family: IPB001092 Basic helix-loop-helix dimerization domain bHLH
UniProtKB/Swiss-Prot: TCFL5_HUMAN, Q9UL49Similarity: Contains 1 bHLH (basic helix-loop-helix) domain |
Function
for TCFL5 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: TCFL5_HUMAN, Q9UL49Function: Putative transcription factor. Isoform 3 may play a role in early spermatogenesis Genatlas biochemistry entry for TCFL5:transcription factor-like 5,specifically expressed in cell nuclei of primary spermatocytes
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TCFL5 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TCFL5
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
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Synthesis Service for TCFL5 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TCFL5 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TCFL5 |
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table
TCFL5 for ontologies About GeneDecksing
|
Pathways & Interactions
for TCFL5 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for TCFL5
STRING Interaction
Network Preview (showing 2 interactants - click image to see more details)
2 Interacting proteins for TCFL5 (Q9UL493 ENSP000003342944) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 5/7 biological process terms (GO ID links to tree view) (see all 7): About this table
TCFL5 for ontologies About GeneDecksing
|
Drugs & Compounds
for TCFL5 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for TCFL5
Search CenterWatch for drugs/clinical trials and news about TCFL5
|
Transcripts
for TCFL5 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for TCFL5 gene: NM_006602.2 Unigene Cluster for TCFL5: Transcription factor-like 5 (basic helix-loop-helix) Hs.126248 [show with all ESTs]Unigene Representative Sequence: BC0655202 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000335351(uc002ydo.3 uc002ydp.3) ENST00000217162(uc002ydq.3)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for TCFL5 (see all 2)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for TCFL5
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: TCFL5 (NM_006602) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for TCFL5 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TCFL5 |
Additional cDNA sequence: AB012124.1 AF070992.1 BC015689.2 BC046933.1 BC065520.1 13 DOTS entries: DT.75107208 DT.100775935 DT.402827 DT.91958693 DT.97834961 DT.100775939 DT.414372 DT.40211115 DT.86858197 DT.92015104 DT.97774279 DT.100746963 DT.86855476 24/136 AceView cDNA sequences (see all 136): BX282585 AI222718 CA419503 AI884611 BU739535 BQ448177 BU741548 BQ718487 AW337242 AI870834 BQ428510 BE302044 CA431192 BU617805 BM769730 AW451768 BQ709994 AL040903 AA398861 BQ637205 NM_006602 AB012124 CA417679 AI285255
GeneLoc Exon Structure
5 Alternative Splicing Database (ASD) splice patterns (SP) for TCFL5 About this scheme
| ExUns: | 1 | ^ | 2a | · | 2b | ^ | 3a | · | 3b | · | 3c | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | ^ | 7 | ^ | 8 | ^ | 9a | · | 9b |
|---|
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| SP1: | | | - | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | - | | - | | - | | - | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | - | | - | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for TCFL5
|
Expression
for TCFL5 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| TCFL5 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
See TCFL5 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for TCFL5
SOURCE GeneReport for Unigene cluster: Hs.126248
UniProtKB/Swiss-Prot: TCFL5_HUMAN, Q9UL49Tissue specificity: Isoform 3 is testis specific. Isoform 2 is pancreas specific
SABiosciences Custom PCR Arrays for TCFL5
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for TCFL5
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat TCFL5 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat TCFL5 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat TCFL5 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TCFL5 |
Orthologs
for TCFL5 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for TCFL5 gene from 4/8 species (see all 8) About this table
ENSEMBL Gene Tree for TCFL5 (if available)
TreeFam Gene Tree for TCFL5 (if available) |
Paralogs
for TCFL5 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for TCFL5 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 20 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for TCFL5 (61472467 - 61493115 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for TCFL5
1 CNV: 31044 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing TCFL5 |
|
Disorders
/ Diseases
for TCFL5 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
TCFL5 for disorders About GeneDecksing
OMIM gene information: 604745
OMIM disorders: --
4  diseases for TCFL5: About MalaCardschagas disease colon carcinoma cholangiocarcinoma carcinoma
Export disorders for TCFL5 gene to outside databases
|
Publications
for TCFL5 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for TCFL5 gene integrated from 9 sources:
(articles sorted by number of sources associating them with TCFL5) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage. (PubMed id 9763657)1, 2, 3, 9 Maruyama O.... Nakamura Y. (1998)
- The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
- Dominant T- and B-cell epitopes in an autoantigen linked to Chagas' disease. (PubMed id 11306602)1, 2 Girones N.... Fresno M. (2001)
- Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration. (PubMed id 22360420)1 Martins-de-Souza D....Bahn S. (2012)
- Regulation of TopBP1 oligomerization by Akt/PKB for c ell survival. (PubMed id 17006541)1 Liu K....Lin W.C. (2006)
- Cha, a basic helix-loop-helix transcription factor involved in the regulation of upstream stimulatory factor activity. (PubMed id 12923186)1 Rodriguez C.I....Fresno M. (2003)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Identification of differentially expressed genes involved in the formation of multicellular tumor spheroids by HT-29 colon carcinoma cells. (PubMed id 17164780)9 Dardousis K....Sachinidis A. (2007)
|
External Searches for TCFL5 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing TCFL5 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing TCFL5 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing TCFL5 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for TCFL5 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for TCFL5 gene:
Search GeneIP for patents involving TCFL5
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for TCFL5 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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variant library, vector shuttling | | | OriGene Custom Antibody Services for TCFL5 | | OriGene Custom Protein Services for TCFL5 | | | OriGene Custom Immunoassay Development | | |
| |
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 | | | TCFL5 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for TCFL5 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat TCFL5 |
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