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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

TBCA Gene

protein-coding   GIFtS: 55
GCID: GC05M077022

tubulin folding cofactor A

(Previous name: tubulin-specific chaperone a )
 Explore 4 diseases affiliated with
TBCA via our new
 Human Malady Compendium 
Biological research products
for TBCA
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Tubulin Folding Cofactor A1 2
Tubulin-Specific Chaperone A1 2
Tubulin-Folding Cofactor A2 3
CFA2 3
TCP1-Chaperonin Cofactor A2 3
Chaperonin Cofactor A2

External Ids:    HGNC: 115791   Entrez Gene: 69022   Ensembl: ENSG000001715307   OMIM: 6100585   UniProtKB: O753473   

Export aliases for TBCA gene to outside databases

Previous GC identifers: GC05M075792 GC05M077221 GC05M077025 GC05M077071 GC05M072194


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for TBCA:
The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to
correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing
and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor
D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are
committed to the native state. This gene encodes chaperonin cofactor A. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: TBCA_HUMAN, O75347
Function: Tubulin-folding protein; involved in the early step of the tubulin folding pathway

Gene Wiki entry for TBCA


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000005.9  NC_018916.1  NT_006713.15  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the TBCA gene promoter:
         GR   Pbx1a   AML1a   POU3F2 (N-Oct-5a)   RelA   POU3F2   NF-kappaB   LCR-F1   GR-alpha   NF-kappaB1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidTBCA promoter sequence
   Search SABiosciences Chromatin IP Primers for TBCA

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat TBCA


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q14.1   Ensembl cytogenetic band:  5q14.1   HGNC cytogenetic band: 5q14.1

TBCA Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TBCA gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M077022:  view genomic region     (about GC identifiers)

Start:
76,986,991 bp from pter      End:
77,164,604 bp from pter
Size:
177,614 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: TBCA_HUMAN, O75347 (See protein sequence)
Recommended Name: Tubulin-specific chaperone A  
Size: 108 amino acids; 12855 Da
Subunit: Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a
quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes
the release of tubulin polypeptides that are committed to the native state
Subcellular location: Cytoplasm, cytoskeleton
1 PDB 3D structure from and Proteopedia for TBCA:
1H7C (3D)    

Explore the universe of human proteins at neXtProt for TBCA: NX_O75347

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O75347

  • TBCA Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_004598.1  
    ENSEMBL proteins: 
     ENSP00000369736   ENSP00000429083   ENSP00000306362   ENSP00000429288   ENSP00000429793  
     ENSP00000429313   ENSP00000429046  
    Reactome Protein details: O75347
    Human Recombinant Protein Products: 
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    ProSpec Recombinant Protein for TBCA
    Uscn Proteins for TBCA

    Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005730nucleolus IDA--
    GO:0005737cytoplasm TAS16130169
    GO:0005874microtubule IEA--
    GO:0015630microtubule cytoskeleton IDA--


    TBCA for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TBCA for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR004226 CofA_tubulin-bd

    Graphical View of Domain Structure for InterPro Entry O75347

    ProtoNet protein and cluster: O75347

    1 Blocks protein family: IPB004226 Tubulin binding cofactor A

    UniProtKB/Swiss-Prot: TBCA_HUMAN, O75347
    Similarity: Belongs to the TBCA family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: TBCA_HUMAN, O75347
    Function: Tubulin-folding protein; involved in the early step of the tubulin folding pathway

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0051082unfolded protein binding IEA--
    GO:0051087chaperone binding TAS8706133


    TBCA for ontologies           About GeneDecksing


    3 GenomeRNAi human phenotypes for TBCA:
     Cell cycle / mitosis defect  Increased cell number in G1, a  Increased gamma-H2AX phosphory 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Chaperonin-mediated protein folding
    Protein folding0.91
    2Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane
    Post-chaperonin tubulin folding pathway0.67
    3Asparagine N-linked glycosylation
    Metabolism of proteins0.15

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    3        Reactome Pathways for TBCA
        Post-chaperonin tubulin folding pathway
    Metabolism of proteins
    Protein folding



    TBCA for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TBCA

    STRING Interaction Network Preview (showing 5 interactants - click image to see 21)

    5/25 Interacting proteins for TBCA (O753473 ENSP000003697364) via UniProtKB, MINT, STRING, and/or I2D (see all 25)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TAF9Q165943, ENSP000002178934I2D: score=1 STRING: ENSP00000217893
    DDIT3P356383I2D: score=1 
    MDM2Q009873I2D: score=1 
    PIK3C2AO004433I2D: score=1 
    RXRBP287023I2D: score=1 
    About this table

    Gene Ontology (GO): 5 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006457protein folding TAS8706133
    GO:0007021tubulin complex assembly IEA--
    GO:0007023post-chaperonin tubulin folding pathway TAS8706133
    GO:0044267cellular protein metabolic process TAS--
    GO:0051084'de novo' posttranslational protein folding TAS--


    TBCA for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for TBCA
    Search CenterWatch for drugs/clinical trials and news about TBCA 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for TBCA gene: 
    NM_004607.2  

    Unigene Cluster for TBCA:

    Tubulin folding cofactor A
    Hs.291212  [show with all ESTs]
    Unigene Representative Sequence: BC067837
    9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000380377(uc003kfh.1) ENST00000518218 ENST00000517679 ENST00000306388(uc003kfi.1)
    ENST00000520361 ENST00000518338 ENST00000517881 ENST00000522370 ENST00000520039


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    Inhib. RNA
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    Additional cDNA sequence: 

    AF038952.1 AK300031.1 AK312207.1 BC018210.1 BC067837.1 BC105285.1 BT007354.1 CR542170.1 
    CR542186.1 

    17 DOTS entries:

    DT.215722  DT.86846553  DT.208079  DT.121283223  DT.97858949  DT.100822339  DT.95279247  DT.120834597 
    DT.97858950  DT.100044565  DT.100641832  DT.120834615  DT.121283207  DT.75100797  DT.120866799  DT.92439561 
    DT.92439565 

    24/227 AceView cDNA sequences (see all 227):

    BG235959 AA371456 BM841505 BQ187731 AI656476 BI492241 AI633758 BX102390 
    AI479967 BF726655 CR542170 AI355069 BE349922 BU070385 CA337813 AI199999 
    AA649574 BG030197 BU676261 BQ436639 BF940058 BF055320 CB068556 BC018210 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TBCA expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GCCGATCCTC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    TBCA expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    1 LifeMap In Vivo Development Anatomical Compartment/Cell 
    Tissue Anatomical Compartment CellCategory (developmental path)
    SomiteThoracic Ventrolateral Dermomyotome LipMuscle Progenitor CellsSkeletal Muscle
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization
    Stem Cell Differentiation: 2 LifeMap Cells 
    NameCategory
    Endoderm-like cells (Generation of hepato...)
    Definitive endoderm-like cells (A scalable, suspensi...)

    See TBCA Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for TBCA

    SOURCE GeneReport for Unigene cluster: Hs.291212
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    In Situ
    Assay Products:
     

     
    Search Advanced Cell Diagnostics for RNAscope RNA in situ hybridization assays for TBCA

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for TBCA gene from 9/28 species (see all 28)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Tbca1 , 5 tubulin cofactor A1, 5 87.04(n)1
    92.59(a)1
      13 (49.22 cM)5
    213711  NM_009321.21  NP_033347.11 
     947889435 
    chicken
    (Gallus gallus)
    Aves TBCA1 tubulin folding cofactor A 80.25(n)
    82.41(a)
      416367  NM_001167729.1  NP_001161201.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    --
    76(a)
    73(a)
    many → 1
    many → 1
    GL343427.1(37828-38154)
    2(13810120-13815708)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.200532 Xenopus laevis transcribed sequence with moderate similarity more 77.35(n)    BJ095977.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc562622 similar to tubulin cofactor a 76.06(n)   394029  BC046032.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG18901 CG1890 50.47(n)
    42.99(a)
      43737  NM_143628.2  NP_651885.1 
    worm
    (Caenorhabditis elegans)
    Secernentea Y39G10AR.206
    Protein Y39G10AR.20
    33(a)
    1 ↔ 1
    I(2312498-2313010)
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons KIS6
    tubulin-specific chaperone A
    39(a)
    1 ↔ 1
    2(12959199-12960810)
    rice
    (Oryza sativa)
    Liliopsida --
    tubulin-specific chaperone A, putative, expressed
    40(a)
    1 ↔ 1
    2(34996873-34998926)


    ENSEMBL Gene Tree for TBCA (if available)
    TreeFam Gene Tree for TBCA (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for TBCA gene

    TBCA for paralogs           About GeneDecksing


    2 Pseudogenes.org Pseudogenes for TBCA
    PGOHUM00000256861 PGOHUM00000241692


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1404 NCBI SNPs in TBCA are shown (see all 1404    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1421081241,2
    --76986520(+) TTGTTC/GATAAG 1 -- ds50010--------
    rs1478095721,2
    --76986548(+) TAAATC/GGTTGG 1 -- ds50010--------
    rs1914988621,2
    --76986561(+) GTGTTC/TTCTGT 1 -- ds50010--------
    rs26522031,2
    C,F,A,--76986580(+) GTCCTG/CGAAAA 1 -- ds500116Minor allele frequency- C:0.46NA WA CSA EA 518
    rs748234521,2
    F,--76986690(+) TTTATA/GTCAGT 1 -- ds50011Minor allele frequency- G:0.08WA 118
    rs1827736781,2
    --76986760(+) TGTTCC/TGTAGG 1 -- ds50010--------
    rs1858974241,2
    --76986787(+) TAATCA/CTACCT 1 -- ds50010--------
    rs26522061,2
    C,F,A,--76986847(+) CCCCAG/ATAGTT 1 -- ds500110Minor allele frequency- A:0.37WA NA CSA EA 373
    rs68884821,2
    C,F,H,--76986900(+) TTGTTA/GTTTGA 1 -- ds500116Minor allele frequency- G:0.11NS EA NA WA CSA 1988
    rs1448299851,2
    --76987012(+) CTATGC/TGGTTT 1 -- ut310--------

    HapMap Linkage Disequilibrium report for TBCA (76986991 - 77164604 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 7 variations for TBCA
         1 CNV: 99178
         6 Indels: 46919 62387 28004 12701 42095 62386

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TBCA for disorders           About GeneDecksing

    OMIM gene information: 610058    OMIM disorders: --

    4 diseases for TBCA:    About MalaCards
    parkinson's disease    osteoporosis    gastric cancer    obesity

    1 disease from the University of Copenhagen DISEASES database for TBCA:
    Osteoporosis
    Human Genome Epidemiology (HuGE) Navigator: TBCA (3 documents)

    Export disorders for TBCA gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for TBCA gene, integrated from 9 sources (see all 26):
    (articles sorted by number of sources associating them with TBCA)
        Utopia: connect your pdf to the dynamic
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    1. Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full- length cDNA cloning. (PubMed id 9653160)1, 2, 3 Mao M.... Chen Z. (1998)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    3. Three-dimensional structure of human tubulin chaperone cofactor A. (PubMed id 12054808)1, 2 Guasch A.... Coll M. (2002)
    4. Pathway leading to correctly folded beta-tubulin. (PubMed id 8706133)1, 3 Tian G....Cowan N.J. (1996)
    5. Proteome-wide identification of ubiquitylation sites b y conjugation of engineered lysine-less ubiquitin. (PubMed id 22053931)1 Oshikawa K....Nakayama K.I. (2012)
    6. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    7. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    8. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
    9. A proteome-wide, quantitative survey of in vivo ubiqui tylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (2011)
    10. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 6902 HGNC: 11579 AceView: TBCA Ensembl:ENSG00000171530 euGenes: HUgn6902
    ECgene: TBCA H-InvDB: TBCA

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for TBCA Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for TBCA gene:
    Search GeneIP for patents involving TBCA

    GeneCards and IP:
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