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Aliases for TBC1D4 Gene

Aliases for TBC1D4 Gene

  • TBC1 Domain Family, Member 4 2 3
  • Akt Substrate Of 160 KDa 2 3 4
  • AS160 3 4 6
  • KIAA0603 4 6
  • TBC (Tre-2, BUB2, CDC16) Domain-Containing Protein 3
  • Acrg Embryonic Lethality Minimal Region Ortholog 3
  • TBC1 Domain Family Member 4 3
  • NIDDM5 3

External Ids for TBC1D4 Gene

Previous GeneCards Identifiers for TBC1D4 Gene

  • GC13M073826
  • GC13M069854
  • GC13M074795
  • GC13M073657
  • GC13M074757
  • GC13M075858
  • GC13M056556

Summaries for TBC1D4 Gene

GeneCards Summary for TBC1D4 Gene

TBC1D4 (TBC1 Domain Family, Member 4) is a Protein Coding gene. Diseases associated with TBC1D4 include diabetes mellitus, noninsulin-dependent, 5. Among its related pathways are Glucose / Energy Metabolism and Clathrin derived vesicle budding. An important paralog of this gene is RABGAP1L.

UniProtKB/Swiss-Prot for TBC1D4 Gene

  • May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

Gene Wiki entry for TBC1D4 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TBC1D4 Gene

Genomics for TBC1D4 Gene

Regulatory Elements for TBC1D4 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for TBC1D4 Gene

75,284,664 bp from pter
75,482,646 bp from pter
197,983 bases
Minus strand

Genomic View for TBC1D4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for TBC1D4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TBC1D4 Gene

Proteins for TBC1D4 Gene

  • Protein details for TBC1D4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    TBC1 domain family member 4
    Protein Accession:
    Secondary Accessions:
    • A7E2X8
    • B4DU25
    • B4E235
    • B6ETN8
    • B6ETN9
    • Q5W0B9
    • Q68D14

    Protein attributes for TBC1D4 Gene

    1298 amino acids
    Molecular mass:
    146563 Da
    Quaternary structure:
    No Data Available
    • Sequence=BAA25529.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for TBC1D4 Gene

    Alternative splice isoforms for TBC1D4 Gene


neXtProt entry for TBC1D4 Gene

Proteomics data for TBC1D4 Gene at MOPED

Post-translational modifications for TBC1D4 Gene

  • Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for TBC1D4 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for TBC1D4 (AS160)

No data available for DME Specific Peptides for TBC1D4 Gene

Domains for TBC1D4 Gene

Suggested Antigen Peptide Sequences for TBC1D4 Gene

Graphical View of Domain Structure for InterPro Entry



  • O60343
  • Contains 2 PID domains.
  • Contains 1 Rab-GAP TBC domain.
genes like me logo Genes that share domains with TBC1D4: view

No data available for Gene Families for TBC1D4 Gene

Function for TBC1D4 Gene

Molecular function for TBC1D4 Gene

UniProtKB/Swiss-Prot Function: May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

Gene Ontology (GO) - Molecular Function for TBC1D4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005096 GTPase activator activity IEA --
GO:0005515 protein binding --
genes like me logo Genes that share ontologies with TBC1D4: view
genes like me logo Genes that share phenotypes with TBC1D4: view

Animal Models for TBC1D4 Gene

MGI Knock Outs for TBC1D4:

Animal Model Products

CRISPR Products

miRNA for TBC1D4 Gene

miRTarBase miRNAs that target TBC1D4

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for TBC1D4

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for TBC1D4 Gene

Localization for TBC1D4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for TBC1D4 Gene

Cytoplasm. Note=Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.

Subcellular locations from

Jensen Localization Image for TBC1D4 Gene COMPARTMENTS Subcellular localization image for TBC1D4 gene
Compartment Confidence
nucleus 3
cytosol 2
plasma membrane 2

Gene Ontology (GO) - Cellular Components for TBC1D4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA --
GO:0030659 cytoplasmic vesicle membrane TAS --
GO:0031988 membrane-bounded vesicle --
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with TBC1D4: view

Pathways for TBC1D4 Gene

genes like me logo Genes that share pathways with TBC1D4: view

Pathways by source for TBC1D4 Gene

1 Cell Signaling Technology pathway for TBC1D4 Gene
1 KEGG pathway for TBC1D4 Gene

Gene Ontology (GO) - Biological Process for TBC1D4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016192 vesicle-mediated transport IMP 22908308
GO:0031339 negative regulation of vesicle fusion IEA --
GO:0032869 cellular response to insulin stimulus IMP 22908308
GO:0043547 positive regulation of GTPase activity IEA --
GO:0061024 membrane organization TAS --
genes like me logo Genes that share ontologies with TBC1D4: view

Compounds for TBC1D4 Gene

(2) Novoseek inferred chemical compound relationships for TBC1D4 Gene

Compound -log(P) Hits PubMed IDs
phosphatidylinositol 58.2 4
glucose 55.7 12
genes like me logo Genes that share compounds with TBC1D4: view

Transcripts for TBC1D4 Gene

Unigene Clusters for TBC1D4 Gene

TBC1 domain family, member 4:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for TBC1D4

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for TBC1D4
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for TBC1D4 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18a · 18b · 18c ^ 19a ·
SP1: - - - - - -
SP3: - - - - - -
SP4: - - - - -
SP5: - - - -
SP6: - - -
SP7: - -

ExUns: 19b ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25a · 25b ^ 26
SP1: -
SP2: - -

Relevant External Links for TBC1D4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for TBC1D4 Gene

mRNA expression in normal human tissues for TBC1D4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for TBC1D4 Gene

This gene is overexpressed in Muscle - Skeletal (6.1) and Adrenal Gland (5.5).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for TBC1D4 Gene

SOURCE GeneReport for Unigene cluster for TBC1D4 Gene Hs.210891

mRNA Expression by UniProt/SwissProt for TBC1D4 Gene

Tissue specificity: Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
genes like me logo Genes that share expressions with TBC1D4: view

In Situ Assay Products

Orthologs for TBC1D4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for TBC1D4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia TBC1D4 35
  • 99.69 (n)
  • 99.61 (a)
TBC1D4 36
  • 100 (a)
(Bos Taurus)
Mammalia TBC1D4 35
  • 89.57 (n)
  • 92.06 (a)
TBC1D4 36
  • 92 (a)
(Canis familiaris)
Mammalia TBC1D4 35
  • 89.15 (n)
  • 90.82 (a)
TBC1D4 36
  • 92 (a)
(Mus musculus)
Mammalia Tbc1d4 35
  • 84.63 (n)
  • 89.45 (a)
Tbc1d4 16
Tbc1d4 36
  • 90 (a)
(Monodelphis domestica)
Mammalia TBC1D4 36
  • 88 (a)
(Ornithorhynchus anatinus)
Mammalia TBC1D4 36
  • 88 (a)
(Rattus norvegicus)
Mammalia Tbc1d4 35
  • 84.81 (n)
  • 89.21 (a)
(Gallus gallus)
Aves TBC1D4 35
  • 75.95 (n)
  • 80.84 (a)
TBC1D4 36
  • 79 (a)
(Anolis carolinensis)
Reptilia TBC1D4 36
  • 78 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.19119 35
tbc1d4 35
  • 69.68 (n)
  • 72.72 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.5191 35
(Danio rerio)
Actinopterygii si:ch211-222g23.4 35
  • 64.22 (n)
  • 65.9 (a)
TBC1D4 36
  • 65 (a)
fruit fly
(Drosophila melanogaster)
Insecta plx 36
  • 26 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes GYL1 36
  • 19 (a)
GYP5 36
  • 19 (a)
Species with no ortholog for TBC1D4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for TBC1D4 Gene

Gene Tree for TBC1D4 (if available)
Gene Tree for TBC1D4 (if available)

Paralogs for TBC1D4 Gene

Paralogs for TBC1D4 Gene

Selected SIMAP similar genes for TBC1D4 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with TBC1D4: view

Variants for TBC1D4 Gene

Sequence variations from dbSNP and Humsavar for TBC1D4 Gene

SNP ID Clin Chr 13 pos Sequence Context AA Info Type MAF
rs11566 -- 75,284,931(+) TTTCC(A/G)AACTT utr-variant-3-prime
rs15167 -- 75,284,973(+) ATCAC(A/T)CCCAT utr-variant-3-prime
rs471822 -- 75,292,056(-) CTACT(A/G)AATAT intron-variant
rs472332 -- 75,352,780(+) AAATG(C/T)TAATT intron-variant
rs476956 -- 75,337,267(+) CCTTC(A/G)TGCCT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for TBC1D4 Gene

Variant ID Type Subtype PubMed ID
nsv521444 CNV Gain 19592680
dgv338n67 CNV Loss 20364138
esv2747669 CNV Deletion 23290073

Relevant External Links for TBC1D4 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TBC1D4 Gene

Disorders for TBC1D4 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for TBC1D4 Gene including...

Search for TBC1D4 Gene in MalaCards »


  • Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5) [MIM:616087]: A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the bodys own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. {ECO:0000269 PubMed:25043022}. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

(1) Novoseek inferred disease relationships for TBC1D4 Gene

Disease -log(P) Hits PubMed IDs
hyperinsulinemia 49.7 4

Relevant External Links for TBC1D4

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with TBC1D4: view

Publications for TBC1D4 Gene

  1. Upregulation of the transcript level of GTPase activating protein KIAA0603 in T cells from patients with atopic dermatitis. (PMID: 15304337) Matsumoto Y. … Oshida T. (FEBS Lett. 2004) 2 3 4 23
  2. Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects. (PMID: 15919790) Karlsson H.K.R. … Wallberg-Henriksson H. (Diabetes 2005) 3 4 23
  3. Rab GTPase-activating protein AS160 is a major downstream effector of protein kinase B/Akt signaling in pancreatic beta-cells. (PMID: 18276765) Bouzakri K. … Halban P.A. (Diabetes 2008) 3 4 23
  4. Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. (PMID: 19077034) Ishibashi K. … Fukuda M. (Genes Cells 2009) 3 4 23
  5. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain. (PMID: 11994271) Kane S. … Lienhard G.E. (J. Biol. Chem. 2002) 2 3 4

Products for TBC1D4 Gene

  • antibodies-online peptides for TBC1D4
  • antibodies-online antibodies for TBC1D4
  • antibodies-online kits for TBC1D4

Sources for TBC1D4 Gene

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