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TARS2 Gene

protein-coding   GIFtS: 50

GC01P148727
threonyl-tRNA synthetase 2, mitochondrial (putative)
(Previous name: threonyl-tRNA synthetase-like 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TARSL1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 6.1.1.3 3
FLJ12528 1, 2
OTTHUMP00000014939 2
TARSL1 2, 3
ThrRS 3
Descriptions
Threonine--tRNA ligase 3
Threonyl-tRNA synthetase-like 1 1, 2, 3
threonine tRNA ligase 2, mitochondrial 2
threonyl-tRNA synthetase 2, mitochondrial 2
threonyl-tRNA synthetase 2, mitochondrial (putative) 2
External Ids
HGNC: 307401
Entrez Gene: 802222
UniProtKB: Q9BW923
Ensembl: ENSG000001433747
Search outside databases for aliases for TARS2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q21.2   Ensembl cytogenetic band:  1q21.2   HGNC cytogenetic band: 1q21.2

TARS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P148727:     (about GC identifiers)

Start:
148,726,517 bp from pter
End:
148,746,702 bp from pter
Size:
20,186 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000001.9  NT_004487.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: SYTM_HUMAN, Q9BW92 (See protein sequence)
Recommended Name: Threonyl-tRNA synthetase, mitochondrial precursor  
Size: 718 amino acids; 81036 Da
Subcellular location: Mitochondrion matrix (By similarity)
Secondary accessions: Q53GW7 Q96I50

REFSEQ proteins: NP_079426.2  

ENSEMBL proteins: 
ENSP00000358060 ENSP00000358050 ENSP00000358049 ENSP00000358048 ENSP00000358047 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (TARS2)
Human Recombinant Proteins from Abnova (TARSL1)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005737 cytoplasm IEA--
GO:0005739 mitochondrion IEA--
GO:0005759 mitochondrial matrix IEA--
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Antibodies for TARS2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for TARS2
Browse R&D Systems for Antibodies
Antibodies from Abcam (TARS2), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (TARSL1)
Novus Biologicals Antibody for TARS2

Assays for TARS2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/8 InterPro domains/families (see all 8 ):
 IPR002314 aa-tRNA-synt_IIb_cons-reg
 IPR002320 Thr-tRNA-synth_IIa
 IPR012675 b-grasp_ferredoxin-like
 IPR006195 aa-tRNA-synth_II_cons-reg
 IPR012947 tRNA_SAD


   GeneDecks  TARS2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9BW92

ProtoNet protein and cluster: Q9BW92

3 Blocks protein families:
IPB002320 Threonyl-tRNA synthetase signature
IPB004095 TGS domain
IPB004154 Anticodon binding domain


UniProtKB/Swiss-Prot: SYTM_HUMAN, Q9BW92
Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TARS2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TARSL1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_025150

              Applied Biosystems Silencer® siRNAs for TARS2

              Sigma-Aldrich siRNA for TARS2  
                     Sigma-Aldrich shRNA for TARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for TARS2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_025150
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_025150
                                 untagged cDNA clone in CMV expression vector: NM_025150 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_025150

UniProtKB/Swiss-Prot: SYTM_HUMAN, Q9BW92
Catalytic activity: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
Enzyme Number (IUBMB): EC 6.1.1.3 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0004829 threonine-tRNA ligase activity IEA--
GO:0005524 ATP binding IEA--
GO:0016874 ligase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TARS2  (Your Favorite Gene powered by Ingenuity) 
 Glycine, Serine and Threonine Metabolism
 Aminoacyl-tRNA Biosynthesis

   GeneDecks  TARS2 for the pathways selected above  
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2 Kegg Pathways  (Kegg details for TARS2):
 hsa00260 Glycine, serine and threonine metabolism
 hsa00970 Aminoacyl-tRNA biosynthesis

   GeneDecks  TARS2 for the pathways selected above  
About GeneDecksing

1 Interacting protein for TARS2 (ENSP000003580603) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
--ENSP000003715493STRING (score=.806)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006435 threonyl-tRNA aminoacylation IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for TARS2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TARS2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TARSL1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_025150

              Sigma-Aldrich siRNA for TARS2  
                     Sigma-Aldrich shRNA for TARS2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_025150  

REFSEQ mRNAs for TARS2 gene: 

NM_025150.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_025150  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_025150
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_025150
                                 untagged cDNA clone in CMV expression vector: NM_025150 

Additional cDNA sequence: 

AK022590.1 AK222814.1 AK225368.1 AK298773.1 AK315507.1 AL512732.1 AY450558.1 BC000541.2 
BC007824.2 BC009997.2 

12 DOTS entries:

DT.308463  DT.100784793  DT.100784790  DT.102828443  DT.121447939  DT.121447959  DT.121447964  DT.121447969 
DT.40308418  DT.92015346  DT.95089088  DT.40225237 

24/165 AceView cDNA sequences (see all 165 ):

CF128265 NM_025150 BU538656 BC009997 BU155618 BE730976 BQ303296 BM837737 
AL520830 AA353210 CF141964 BQ940615 CK430228 BC007824 AA552157 BM561906 
BX281571 BM974316 CA426625 BC000541 BU543143 AW627780 BM770498 BQ230411 

Unigene Cluster for TARS2:

Threonyl-tRNA synthetase 2, mitochondrial (putative)
Hs.288974  [show with all ESTs]
Unigene Representative Sequence: BC007824


GeneLoc Exon Structure

5/14 Alternative Splicing Database (ASD) splice patterns (SP) for TARS2 (see all 14 )

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c · 7d ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c ^
SP1:                                -           -                                         -     -     -                                                         
SP2:                                -           -                                   -     -     -     -     -     -     -     -     -     -                     
SP3:                                -           -                                         -     -     -                 -     -                                 
SP4:                                                                                -     -     -     -                 -     -     -     -                     
SP5:                                                                                      -     -     -                 -     -     -     -                     

ExUns: 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b
SP1:                                                            
SP2:                                                            
SP3:                                                            
SP4:                                                            
SP5:                                                            

About this scheme

ECgene alternative splicing isoforms for TARS2

5 Ensembl transcripts including schematic representations:
ENST00000369064  ENST00000369054  ENST00000369053  ENST00000369052  ENST00000369051  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

TARS2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for TARS2

1 / 2

5 probe-sets matching TARS2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

70937_at2 U95-E 1 1.00 1.00 0.80 1.00 -- -- -- -- --

77595_at2 U95-E 2 1.00 0.66 0.94 0.96 -- -- -- -- --

77221_s_at2 U95-E 2 1.00 0.50 0.87 1.04 -- -- -- -- --

221189_s_at2 U133-A 3 1.00 0.64 -- -- -- -- -- -- --

221189_s_at2 U133Plus2 3 1.00 0.64 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TCTGCTCTCC

SOURCE GeneReport for Unigene cluster: Hs.288974
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for TARS2 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TARS21   -- threonyl-tRNA synthetase 2, mitochondrial (putative) 88.07(n)
87.33(a)
608006  XM_845067.1  XP_850160.1 
chimpanzee
(Pan troglodytes)
TARS21   -- threonyl-tRNA synthetase 2, mitochondrial (putative) 99.81(n)
99.86(a)
746922  XM_001168944.1  XP_001168944.1 
cow
(Bos taurus)
TARS21   -- threonyl-tRNA synthetase 2, mitochondrial (putative) 88.3(n)
88.02(a)
511740  XM_866940.2  XP_872033.1 
rat
(Rattus norvegicus)
Tars21   -- threonyl-tRNA synthetase 2, mitochondrial (putative) 83.38(n)
83.15(a)
310672  NM_001014040.1  NP_001014062.1 
mouse
(Mus musculus)
Tars21, 5 35
threonyl-tRNA synthetase 2, mitochondrial (putative)1, 5 83.75(n)1
84.26(a)1
718071  NM_027931.21  NP_082207.11 
 AI4292085  AK0115405  (see all 21)
About this table        Species with no ortholog for TARS2

ENSEMBL Gene Tree for TARS2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for TARS2 gene
TARSL22  MRPL392  TARS2  

Pseudogenes
1 related pseudogene


(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/136 NCBI SNPs in TARS2 are shown (see all 136 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 62)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs94360091,2
A,C,F,H148726050(+) aagttA/Taataa 1 -- ng51 tfbs34Minor allele frequency- T:0.46EU EA WA 418
rs66647031,2
A,C,F,H148725711(+) TTACAC/GTCATT 1 -- ng514Minor allele frequency- G:0.46EU EA WA 416
rs49709641,2
A,C,F,H148724653(+) AAAGAA/GAACCA 1 -- ng518Minor allele frequency- N:0.00EA NA EU WA 632
rs49709221,2
A,C,F,H148724718(+) TCTGTA/CAGATT 1 -- ng514Minor allele frequency- C:0.45EU EA WA 418
--
rs283728731,2
F148726318(+) GGGGAA/-TAGCG 1 -- ng512Minor allele frequency- -:0.37MN EA 1252
--
rs283728741,2
F148726520(+) ATCTGTTTG/-  
        
AGGAT
1 -- ng512Minor allele frequency- -:0.12MN EA 1252
rs22752451,2
C,F,H148726972(-) GGAGGG/AGTCGA 1 T/T syn1 ese38Minor allele frequency- A:0.12EA NA EU WA 2064
rs8715271,2
A,C,F,H,O148728712(+) AAGACC/GAAACA 1 -- int115Minor allele frequency- G:0.34NA PA NS EU EA WA 920
rs75528831,2
A,C,F,H148732546(+) TTAGTA/GAAGGG 1 -- int17Minor allele frequency- G:0.18EU EA WA NA 546
rs112053851,2
A,C,F,H148743140(+) AGATgG/Agtggt 1 -- int14Minor allele frequency- A:0.22EU EA WA 416
About this table

HapMap Linkage Disequilibrium images for TARS2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 612805

Human Genome Epidemiology Navigator: TARS2 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for TARS2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 80222 HGNC: 30740 AceView: FLJ12528 Ensembl:ENSG00000143374 euGenes: HUgn80222
ECgene: TARS2 H-InvDB: TARS2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for TARS2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for TARS2-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for TARS2 Antibodies for TARS2
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for TARS2 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant & Natural Proteins  

 Recombinant Proteins (TARSL1)
 Antibodies (TARSL1)
 Chimera RNAi (TARSL1)
 Custom Service for Mouse Mab
 Custom Service for Rabbit Pab from Full-length Protein
  
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 Recombinant Proteins
(TARS2)
 Antibodies (TARS2)
 Search OriGene for TARS2
 Search Tocris compounds for TARS2




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for TARS2

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