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TAGAP Gene

protein-coding   GIFtS: 50

GC06M159375
T-cell activation RhoGTPase activating protein
(Previous name: T-cell activation GTPase activating protein )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FKSG15 2
FLJ32631 1, 2
FLJ39771 2
MGC133247 2
MGC27381 2
OTTHUMP00000017514 2
OTTHUMP00000017515 2
OTTHUMP00000017516 2
TAGAP1 2, 3
Descriptions
T-cell activation GTPase activating protein 1
T-cell activation GTPase-activating protein 3
T-cell activation Rho GTPase-activating protein 2
T-cell activation RhoGTPase activating protein 2
External Ids
HGNC: 156691
Entrez Gene: 1172892
UniProtKB: Q8N1033
Ensembl: ENSG000001646917
Search outside databases for aliases for TAGAP gene

Previous GC identifers: GC06M158897 GC06M159329 GC06M159365 GC06M159426

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for TAGAP:
This gene encodes a protein that may function as a Rho GTPase-activating protein. Transcript
variants encoding distinct isoforms have been identified. [provided by RefSeq]

UniProtKB/Swiss-Prot: TAGAP_HUMAN, Q8N103
Function: May function as a GTPase-activating protein and may play important roles during T-cell
activation

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TAGAP gene  

Entrez Gene cytogenetic band: 6q25.3   Ensembl cytogenetic band:  6q25.3   HGNC cytogenetic band: 6q25.3

TAGAP Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06M159375:     (about GC identifiers)

Start:
159,376,012 bp from pter
End:
159,386,172 bp from pter
Size:
10,161 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000006.10  NT_007422.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TAGAP_HUMAN, Q8N103 (See protein sequence)
Recommended Name: T-cell activation Rho GTPase-activating protein  
Size: 731 amino acids; 80703 Da
Secondary accessions: Q2NKM8 Q8NI40 Q96KZ2 Q96QA2
Alternative splicing: 4 isoforms:  Q8N103-1   Q8N103-2   Q8N103-3   Q8N103-4   

REFSEQ proteins (3 alternative transcripts): 
NP_473455.2  NP_620165.1  NP_687034.1  


ENSEMBL proteins: 
ENSP00000356033 ENSP00000356028 ENSP00000322650 ENSP00000340217 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (TAGAP)
Human Recombinant Proteins from Abnova (TAGAP)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005622 intracellular IEA--
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Antibodies for TAGAP: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Antibodies from Abcam (TAGAP), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (TAGAP)
Novus Biologicals Antibodies for TAGAP

Assays for TAGAP: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR000198 RhoGAP


   GeneDecks  TAGAP for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q8N103

ProtoNet protein and cluster: Q8N103

1 Blocks protein family: IPB000198 RhoGAP domain

UniProtKB/Swiss-Prot: TAGAP_HUMAN, Q8N103
Similarity: Contains 1 Rho-GAP domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TAGAP)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_152133

              Applied Biosystems Silencer® siRNAs for TAGAP

              Sigma-Aldrich siRNA for TAGAP  
                     Sigma-Aldrich shRNA for TAGAP  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_054114
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_054114
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_054114 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_054114

UniProtKB/Swiss-Prot: TAGAP_HUMAN, Q8N103
Function: May function as a GTPase-activating protein and may play important roles during T-cell
activation

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005085 guanyl-nucleotide exchange factor activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/68 Interacting proteins for TAGAP (ENSP000003560333) via UniProtKB, MINT, and/or STRING (see all 68 )
InteractantInteraction Details
GeneCardExternal ID(s)
PLEKHG5ENSP000003669773STRING (score=.931)
ABRENSP000003039093STRING (score=.899)
AKAP13ENSP000003547183STRING (score=.899)
ARHGAP4ENSP000003590453STRING (score=.899)
ARHGDIAENSP000002693213STRING (score=.899)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0007165 signal transduction IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for TAGAP

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TAGAP)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_152133

              Sigma-Aldrich siRNA for TAGAP  
                     Sigma-Aldrich shRNA for TAGAP  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_054114  NM_138810  NM_152133  

REFSEQ mRNAs for TAGAP gene (3 alternative transcripts): 

NM_054114.3   NM_138810.2   NM_152133.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_054114  NM_138810  NM_152133  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_054114
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_054114
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_054114 

Additional cDNA sequence: 

AF385429.1 AF385430.1 AK057193.1 AK097090.1 AK298686.1 AL832662.1 BC015859.1 BC111731.1 

8 DOTS entries:

DT.40312762  DT.40308071  DT.40125502  DT.100673318  DT.100776153  DT.100642280  DT.101979617  DT.97772033 

24/56 AceView cDNA sequences (see all 56 ):

AK097090 NM_054114 AF385429 AK057193 BF591040 NM_138810 AA352840 AI253155 
BM991735 BX489278 AF385430 AW576600 BE676780 BX342972 AI349366 AA251806 
AW008517 AI766650 NM_152133 AI356482 AI760827 BF928548 CA306993 AW407984 

highest scoring ESTs for TAGAP:

AF385429 AF385430 AI760827 AK097090 AL832662 AW408019 AW502767 BC015859 BG536356 CA407943 

Unigene Cluster for TAGAP:

T-cell activation RhoGTPase activating protein
Hs.529984  [show with all ESTs]
Unigene Representative Sequence: NM_054114


GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for TAGAP (see all 9 )

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c · 5d ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c ^ 10 ^ 11
SP1:                                            -     -     -                             -                             -               
SP2:                                            -     -     -     -     -                 -                             -               
SP3:                                            -     -     -                             -                                             
SP4:                                            -     -                                   -                                             
SP5:                                            -     -     -                                                                           

About this scheme

ECgene alternative splicing isoforms for TAGAP

4 Ensembl transcripts including schematic representations:
ENST00000367066  ENST00000367061  ENST00000326965  ENST00000338313  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

TAGAP expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for TAGAP

1 / 2 / 3

7 probe-sets matching TAGAP gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
82887_at2, 3 U95-E 1 1.00 1.00 1.00 1.00 AI760827 0.80 1.00 0.91 1

234050_at2, 3 U133-B 1 1.00 1.00 -- -- AK025272 0.20 1.00 0.72 1

229723_at2, 3 U133-B 1 1.00 1.00 -- -- BF591040 0.40 1.00 0.76 1

1552541_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

234050_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

229723_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1552542_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  TAGAP for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ATATCCTATC

SOURCE GeneReport for Unigene cluster: Hs.529984
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for TAGAP gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TAGAP1   -- T-cell activation RhoGTPase activating protein 79.25(n)
74.14(a)
484059  XM_541176.2  XP_541176.2 
chimpanzee
(Pan troglodytes)
TAGAP1   -- T-cell activation RhoGTPase activating protein 99.45(n)
99.45(a)
463105  XM_001147837.1  XP_001147837.1 
cow
(Bos taurus)
TAGAP1   -- T-cell activation GTPase activating protein 74.57(n)
68.02(a)
509236  NM_001076823.1  NP_001070291.1 
rat
(Rattus norvegicus)
Tagap1   -- T-cell activation GTPase activating protein 75.94(n)
69.94(a)
308097  XM_217791.4  XP_217791.4 
mouse
(Mus musculus)
LOC1000389461   -- similar to T-cell activation Rho GTPase-activating more 75.97(n)
69(a)
100038946  XM_001471952.1  XP_001472002.1 
About this table        Species with no ortholog for TAGAP

ENSEMBL Gene Tree for TAGAP
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for TAGAP gene
ARHGAP202  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/67 NCBI SNPs in TAGAP are shown (see all 67 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 84)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 6 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs3808851,2
A,C,F,H,O159387339(+) TAACTC/TAGCGG 3 -- ng3112Minor allele frequency- T:0.19MN EU EA WA NA 1166
rs125288931,2
A,C,F,H159379819(+) GTAGTG/TTCCCA 3 -- int1 ng5115Minor allele frequency- T:0.25EU EA WA NA 1388
rs94575441,2
A,C,F,H159387504(+) CTCCAA/TAGTGA 3 -- ng314Minor allele frequency- T:0.19EU EA WA 420
--
rs47092641,2
A,C,F,H159376199(+) ACCCAC/AATTTA 2 -- ut315Minor allele frequency- A:0.08NA EU WA 356
--
rs122151141,2
A,C,F,H159375594(+) ATAATT/CGAACA 2 -- ng51 ese37Minor allele frequency- C:0.39NA EU EA WA 562
rs47092661,2
A,C,F,H159376254(+) TGCTAC/TGGCCC 2 -- ut31 ese37Minor allele frequency- T:0.25NA EU EA WA 554
rs47092671,2
A,C,F,H159376363(+) GCAAGA/GCTGCT 2 -- ut31 ese311Minor allele frequency- G:0.26NA EU EA WA 980
rs17380741,2
A,C,F,H159385965(-) GAGTTA/GTGCTG 3 -- ut5118Minor allele frequency- G:0.40MN NA EU EA WA 1756
--
rs104558551,2
A,F,H159375822(+) TTTTTA/TATGGT 2 -- ng514Minor allele frequency- T:0.25EU EA WA 406
rs69087441,2
C,F,H159386592(+) TGTCAA/GTATAG 3 -- ng314Minor allele frequency- G:0.02EU EA WA 416
About this table

HapMap Linkage Disequilibrium images for TAGAP (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 609667

Human Genome Epidemiology Navigator: TAGAP (4 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
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10/11 PubMed articles for TAGAP gene (see all 11 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 117289 HGNC: 15669 AceView: TAGAP Ensembl:ENSG00000164691 euGenes: HUgn117289
ECgene: TAGAP H-InvDB: TAGAP
(According to HUGE)
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  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for TAGAP:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for TAGAP-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for TAGAP Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for TAGAP Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Antibodies for TAGAP

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