TAF9 Gene
protein-coding GIFtS : 66
GC05M068683
TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa (Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, G, 32kD )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database (Previous symbol: TAF2G )
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Aliases & Descriptions for TAF9
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases AD-004 1 , 2 AK6 1 , 2 CGI-137 1 , 2 CINAP 1 , 2 CIP 2 MGC1603 1 , 2 MGC3647 1 , 2 MGC5067 1 , 2 MGC:1603 2 MGC:3647 2 MGC:5067 2 OTTHUMP00000125186 2 OTTHUMP00000125196 2 STAF31/32 3 TAF2G 2 , 3 , 5 TAFII-31 3 TAFII-32 3 TAFII31 1 , 2 , 3 TAFII32 1 , 2 , 3 , 5 TAFIID32 1 , 2
Descriptions RNA polymerase II TBP-associated factor subunit G 3 TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa 2 TAF9 RNA polymerase II, TATA box binding protein-associated factor 2 TAF9 RNA polymerase II, TATA box binding protein-associated factor, 32kDa 1 TATA box binding protein (TBP)-associated factor, RNA polymerase II, G, 32kD 1 Transcription initiation factor TFIID 31 kDa subunit 3 adenylate kinase 6 2 adrenal gland protein AD-004 2 coilin interacting protein 2 coilin-interacting nuclear ATPase protein 2 transcription initiation factor TFIID 31 kD subunit 2
Search outside databases for aliases for TAF9 genePrevious GC identifers: GC05M068800 GC05M070213 GC05M068617 GC05M068663
Summaries for TAF9 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for TAF9 : Initiation of transcription by RNA polymerase II requires the activities of more than 70polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID),which binds to the core promoter to position the polymerase properly, serves as the scaffold forassembly of the remainder of the transcription complex, and acts as a channel for regulatorysignals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarilyconserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basaltranscription, serve as coactivators, function in promoter recognition or modify generaltranscription factors (GTFs) to facilitate complex assembly and transcription initiation. Thisgene encodes one of the smaller subunits of TFIID that binds to the basal transcription factorGTF2B as well as to several transcriptional activators such as p53 and VP16. A similar butdistinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified onchromosome 19. Alternative splicing results in multiple transcript variants encoding differentisoforms. [provided by RefSeq] UniProtKB/Swiss-Prot: TAF9_HUMAN, Q16594 Function : Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activationas well as repression of distinct but overlapping sets of genes. May have a role in generegulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID)complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGAtranscription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNApolymerase II-mediated transcription
Gene Wiki entry for TAF9
Genomic Location for TAF9
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the TAF9 gene Entrez Gene cytogenetic band: 5q11.2-q13.1 Ensembl cytogenetic band: 5q13.2 HGNC cytogenetic band: 5q11.2-q13.1 TAF9 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 5 GeneLoc Exon Structure
GeneLoc location for GC05M068683:
(about GC identifiers )
Start:
68,683,309 bp from pter
End:
68,701,596 bp from pter
Size:
18,288 bases
Orientation:
minus strand
1 alternative location : Chr 5-,c5_H2 69,107,682-69,125,972
RefSeq DNA sequence: NC_000005.8 NT_006713.14 NT_113801.1 Proteins for TAF9
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: TAF9_HUMAN, Q16594 (See
protein sequence )Recommended Name: Transcription initiation factor TFIID subunit 9 Size : 264 amino acids; 28974 Da
Subunit : Component of TFIID, the TATA-binding protein-free TAF complex (TFTC), the PCAF complex andthe STAGA transcription coactivator-HAT complex. The PCAF complex consists at least ofTADA2L/ADA2, SUPT3H/SPT3, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30,TAF12/TAFII20, TAF9/TAFII31 and TRRAP. The STAGA transcription coactivator-HAT complex consists atleast of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. BindsN-terminal domain of TP53/p53 which is essential for transcription. Binds TFIIB and the Herpessimplex virus activator VP16. Forms a heterodimer with TAF6/TAFII80 in a complex with theTAF4B/TAFII105-TAF12/TAFII20 heterodimer. Also interacts with TAF5. Binds directly DNA. IncreasedDNA binding when complexed with TAF6/TAFII80
Subcellular location : Nucleus
Secondary accessions : Q5U0D1 Q9BTS1
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (4 alternative transcripts):
NP_001015891.1 NP_001015892.1 NP_003178.1 NP_057367.1 ENSEMBL proteins: ENSP00000370193 ENSP00000217893 Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: TAF9 3 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for TAF9: Assays for TAF9:
Protein
Domains/ Families for TAF9(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q16594 ProtoNet protein and cluster: Q16594
1 Blocks protein family : IPB003162 Transcription factor TAFII-31 UniProtKB/Swiss-Prot: TAF9_HUMAN, Q16594 Similarity : Belongs to the TAF9 family
Gene Function for TAF9
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4 ): NM_003187 Applied Biosystems Silencer ® siRNAs for TAF9 Sigma-Aldrich siRNA and siRNA Panels for TAF9 Sigma-Aldrich shRNA for TAF9 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 4 ): NM_001015891                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 4 ): NM_001015891                                  untagged cDNA clones in CMV expression vector (see all 4 ): NM_001015891  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_001015892 UniProtKB/Swiss-Prot: TAF9_HUMAN, Q16594 Function : Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activationas well as repression of distinct but overlapping sets of genes. May have a role in generegulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID)complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGAtranscription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNApolymerase II-mediated transcription Induction : 6 to 8-fold by apoptotic signals
5/9 Gene Ontology (GO) molecular function terms (links to tree view) (see all 9
):
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Pathways & Interactions for TAF9
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for TAF9 (Your Favorite Gene powered by Ingenuity) Gene Network CentralTM Interacting Genes and Proteins Network for TAF9 5/109 Interacting proteins for TAF9 (ENSP00000370193 3 Q16594 1 , 2 ) via UniProtKB, MINT, and/or STRING (see all 109
)About this table 4 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0006350 transcription
IEA -- GO:0006355 regulation of transcription, DNA-dependent
IEA -- GO:0006367 transcription initiation from RNA polymerase II promoter
EXP 8946909 GO:0006368 RNA elongation from RNA polymerase II promoter
EXP 9405375
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Drugs & Compounds for TAF9 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for TAF9
Transcripts for TAF9(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
Sigma-Aldrich ,
OriGene ,
Tagged/untagged cDNA clones from
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4 ): NM_003187 Sigma-Aldrich siRNA and siRNA Panels for TAF9 Sigma-Aldrich shRNA for TAF9 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_001015891 NM_001015892 NM_003187 NM_016283
REFSEQ mRNAs for TAF9 gene (4 alternative transcripts): NM_001015891.1 NM_001015892.1 NM_003187.4 NM_016283.4
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001015891 NM_001015892 NM_003187 NM_016283
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 4 ): NM_001015891                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 4 ): NM_001015891                                  untagged cDNA clones in CMV expression vector (see all 4 ): NM_001015891  
Additional cDNA sequence: AF110777.1 AF151895.1 AJ878880.1 AJ878881.1 BC003400.1 BC007349.2 BC007426.2 BC033320.1 BT019652.1 CR606803.1 CR610818.1 U21858.1 U25112.1 U30504.1
24/28 DOTS entries (see all 28
): DT.100819169 DT.452110 DT.120842325 DT.95258926 DT.100801079 DT.120842183 DT.443071 DT.70103909 DT.40307992 DT.422686 DT.120842396 DT.419165 DT.121504121 DT.100703220 DT.100819170 DT.120842191 DT.97862317 DT.100685961 DT.100819171 DT.65286751 DT.95192818 DT.95193472 DT.120842176 DT.120842177
24/450 AceView cDNA sequences (see all 450
):BU951907 BQ020392 CA421215 BU622298 AA321648 BI086372 BU521711 BM915623 AA255929 AI559878 BQ772517 BG055177 BQ008630 AV727986 AA181113 BG942858 AA743298 T36197 AW470751 BG940323 BU620877 AU102679 AA632383 AA243818
highest scoring ESTs for TAF9 :AA046950 BU193943 BX117839 CD171997 U21858 AA015966 AA026887 AA026888 AA029766 AA031331
Unigene Cluster for TAF9: TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa Hs.653163 [show with all ESTs ] Unigene Representative Sequence: BC033320 GeneLoc Exon Structure 5/10 Alternative Splicing Database (ASD) splice patterns (SP) for TAF9 (see all 10
) ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c · 7d · 7e · 7f · 7g ^ 8 ^ 9a · 9b ^ 10 SP1 :         -   -   -   -   -   -   -     -   -   -   -   -   -   -       -     SP2 :   -   -   -   -   -   -   -   -   -   -                           SP3 :         -   -   -   -   -   -   -                           SP4 :           -   -   -   -   -   -                           SP5 :               -   -   -   -                          
About this scheme ECgene alternative splicing isoforms for TAF9 2 Ensembl transcripts including schematic representations : ENST00000328663
ENST00000217893
Expression for TAF9
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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TAF9 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for TAF9 1 / 2 / 3
5 probe-sets matching TAF9 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: CTGGATGTTTSOURCE GeneReport for Unigene cluster: Hs.653163 Expression variation in blood from EXPOLDB for TAF9
Orthologs for TAF9
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for TAF9 gene from 5/17 species (see all 17
)
About this table Species with no ortholog for TAF9 ENSEMBL Gene Tree for TAF9 Paralogs for TAF9 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for TAF9 gene TAF9B 2 ENSG00000215760 2 Pseudogenes 1 related pseudogene
SNPs/Variants for TAF9 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for TAF9 (up to first 250kb)
Disorders & Mutations for TAF9
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 600822
Medical News for TAF9 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for TAF9 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/56 PubMed articles for TAF9 gene (see all 56
): Evolutionary conservation of human TATA-binding-polypeptide- associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors. (PubMed id 7667268) 1, 3, 4 Hisatake K.... Roeder R.G. (1995) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983) 3, 4 Olsen J.V....Mann M. (2006) Core promoter binding by histone-like TAF complexes. (PubMed id 15601843) 3, 4 Shao H.... Dikstein R. (2005) TAF9b (formerly TAF9L) is a bona fide TAF that has unique and overlapping roles with TAF9. (PubMed id 15899866) 3, 4 Frontini M.... Tora L. (2005) The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus. (PubMed id 15630091) 2, 3 Ren H....Su X.D. (2005) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. (PubMed id 11564863) 3, 4 Martinez E....Roeder R.G. (2001) Mapping and mutational analysis of the human TAF2G gene encoding a p53 cofactor. (PubMed id 10191103) 2, 3 Evans S.C....Lozano G. (1999) Histone-like TAFs within the PCAF histone acetylase complex. (PubMed id 9674425) 3, 4 Ogryzko V.V.... Nakatani Y. (1998) The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily. (PubMed id 9885574) 3, 4 Vassilev A....Nakatani Y. (1998)
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Genome Databases showing TAF9
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing TAF9
(According to HUGE )
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Specialized Databases showing TAF9 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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-- Services for TAF9 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for TAF9:
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Aliases for TAF9
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GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009