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TAF7L Gene

protein-coding   GIFtS: 53

GC0XM100409
TAF7-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50kDa
(Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, Q )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TAF2Q)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
CT40 1, 2, 3
FLJ23157 2
OTTHUMP00000023675 2
TAF2Q 2, 3, 5
dJ738A13.1 2
Descriptions
Cancer/testis antigen 40 2, 3
RNA polymerase II TBP-associated factor subunit Q 3
TAF7-like RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 50 kD 2
TAF7-like RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 50kDa 2
TAF7-like RNA polymerase II, TATA box binding
protein-associated factor, 50kDa 1
TATA box binding protein (TBP)-associated factor, RNA
polymerase II, Q 1, 2
TATA box binding protein-associated factor, RNA polymerase
II, Q 2
TATA box-binding protein-associated factor 50 kDa 3
TBP-associated factor, RNA polymerase II, Q 2
Transcription initiation factor TFIID 50 kDa subunit 3
External Ids
HGNC: 115481
Entrez Gene: 544572
UniProtKB: Q5H9L43
Ensembl: ENSG000001023877
Search outside databases for aliases for TAF7L gene

Previous GC identifers: GC0XM095804 GC0XM097484 GC0XM098558 GC0XM099295 GC0XM100329

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for TAF7L:
This gene is similar to a mouse gene that encodes a TATA box binding protein-associated factor, and
shows testis-specific expression. [provided by RefSeq]

UniProtKB/Swiss-Prot: TAF7L_HUMAN, Q5H9L4
Function: Probably functions as a spermatogensis-specific component of the DNA-binding general
transcription factor complex TFIID, a multimeric protein complex that plays a central role in
mediating promoter responses to various activators and repressors. May play a role in
spermatogenesis (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TAF7L gene  

Entrez Gene cytogenetic band: Xq22.1   Ensembl cytogenetic band:  Xq22.1   HGNC cytogenetic band: Xq22.1

TAF7L Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XM100409:     (about GC identifiers)

Start:
100,409,897 bp from pter
End:
100,434,701 bp from pter
Size:
24,805 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000023.9  NT_011651.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TAF7L_HUMAN, Q5H9L4 (See protein sequence)
Recommended Name: Transcription initiation factor TFIID subunit 7-like  
Size: 462 amino acids; 52588 Da
Subunit: TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors
(TAFs). TAF7L may replace TAF7 in a spermatogenensis-specific form of TFIID. Interacts with TBP;
the interaction occurs in a sub-population of cells (pachytene and haploid round spermatids) and
is developmentally regulated through differential intracellular localization of the two proteins.
Interacts with TAF1 (By similarity)
Subcellular location: Nucleus (By similarity). Cytoplasm (By similarity). Note=Cytoplasmic in
spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into
the nuclei of pachytene spermatocytes and round spermatids (By similarity)
Secondary accessions: Q5H9L6 Q86XI4 Q9BXU5 Q9H5R0
Alternative splicing: 3 isoforms:  Q5H9L4-1   Q5H9L4-2   Q5H9L4-3   

REFSEQ proteins: NP_079161.2  

ENSEMBL proteins: 
ENSP00000349235 ENSP00000361998 ENSP00000361996 ENSP00000320283 


Human Recombinant Proteins 
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Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (TAF7L)
Human Recombinant Proteins from Abnova (TAF7L)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

4 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
GO:0005669 transcription factor TFIID complex IEA--
GO:0005730NOT nucleolus IDA18029348
GO:0005737 cytoplasm IEA--
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Antibodies for TAF7L: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for TAF7L
Browse R&D Systems for Antibodies
Antibodies from Abcam (TAF7L), each with their AbpromiseSM
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Search Novus for antibodies for TAF7L

Assays for TAF7L: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro domain/family:
 IPR006751 TAFII55_N


   GeneDecks  TAF7L for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q5H9L4

ProtoNet protein and cluster: Q5H9L4

1 Blocks protein family: IPB006751 TAFII55 protein conserved region

UniProtKB/Swiss-Prot: TAF7L_HUMAN, Q5H9L4
Similarity: Belongs to the TAF7 family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TAF7L)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TAF7L)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024885

              Applied Biosystems Silencer® siRNAs for TAF7L

              Sigma-Aldrich siRNA for TAF7L  
                     Sigma-Aldrich shRNA for TAF7L  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for TAF7L
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024885
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024885
                                 untagged cDNA clone in CMV expression vector: NM_024885 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_024885

UniProtKB/Swiss-Prot: TAF7L_HUMAN, Q5H9L4
Function: Probably functions as a spermatogensis-specific component of the DNA-binding general
transcription factor complex TFIID, a multimeric protein complex that plays a central role in
mediating promoter responses to various activators and repressors. May play a role in
spermatogenesis (By similarity)

2 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Taf7l):

endocrine/exocrine glandreproductive system

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016251 general RNA polymerase II transcription factor activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Millipore Pathway for TAF7L
 Transcription Ligand-Dependent Transcription of Retinoid-Target genes

   GeneDecks  TAF7L for the pathways selected above  
About GeneDecksing

2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TAF7L  (Your Favorite Gene powered by Ingenuity) 
 Glucocorticoid Receptor Signaling
 Estrogen Receptor Signaling

   GeneDecks  TAF7L for the pathways selected above  
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1 Kegg Pathway  (Kegg details for TAF7L):
 hsa03022 Basal transcription factors

   GeneDecks  TAF7L for the pathways selected above  
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 Gene Network CentralTM Interacting Genes and Proteins Network for  TAF7L 


3 Interacting proteins for TAF7L (ENSP000003619983) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
TBPENSP000002303543STRING (score=.923)
TAF7ENSP000003127093STRING (score=.808)
TAF1ENSP000002760723STRING (score=.71)
About this table

5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0006350 transcription IEA--
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0006367 transcription initiation from RNA polymerase II promoter IEA--
GO:0007275 multicellular organismal development IEA--
GO:0007283 spermatogenesis IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for TAF7L

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TAF7L)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TAF7L)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024885

              Sigma-Aldrich siRNA for TAF7L  
                     Sigma-Aldrich shRNA for TAF7L  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_024885  

REFSEQ mRNAs for TAF7L gene: 

NM_024885.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_024885  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024885
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024885
                                 untagged cDNA clone in CMV expression vector: NM_024885 

Additional cDNA sequence: 

AF285595.1 AK026810.1 AK308261.1 BC043391.1 

3 DOTS entries:

DT.95341214  DT.121312664  DT.95351052 

23 AceView cDNA sequences:

BC043391 AI699474 AA902216 AI962851 AW590415 AI220545 AW236846 NM_024885 
AI190862 AA885108 AW117310 AK026810 AW183175 AI950228 AA401392 BE504739 
AA405087 BX100254 AA401416 AF285595 AI025202 BF508107 AW235517 

highest scoring ESTs for TAF7L:

BC043391 AA401416 AA405087 AK026810 NM_024885 AA401392 AA885108 AA902216 AI025202 AI190862 

Unigene Cluster for TAF7L:

TAF7-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50kDa
Hs.223806  [show with all ESTs]
Unigene Representative Sequence: BC043391


GeneLoc Exon Structure

4 Ensembl transcripts including schematic representations:
ENST00000356784  ENST00000372907  ENST00000372905  ENST00000324762  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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TAF7L expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for TAF7L

1 / 2 / 3

5 probe-sets matching TAF7L gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

52496_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AA401416 0.60 1.00 0.82 1

224380_s_at2, 3 U133-B 1 1.00 1.00 -- -- AF285595 0.60 0.75 0.68 1

220325_at2, 3 U133-A 1 1.00 1.00 -- -- NM_024885 0.60 1.00 0.82 1

224380_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

220325_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CTTCTAAAGG

SOURCE GeneReport for Unigene cluster: Hs.223806

UniProtKB/Swiss-Prot: TAF7L_HUMAN, Q5H9L4
Tissue specificity: Testis-specific

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for TAF7L gene from 5/6 species (see all 6 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TAF7L1   -- TAF7-like RNA polymerase II, TATA box binding protein more 80.51(n)
68.75(a)
480985  XM_538106.2  XP_538106.2 
cow
(Bos taurus)
TAF7L1   -- TAF7-like RNA polymerase II, TATA box binding protein more 79.03(n)
64.62(a)
615711  XM_867587.1  XP_872680.1 
rat
(Rattus norvegicus)
Taf7l-ps11   -- TAF7-like RNA polymerase II, TATA box binding protein more 76.22(n)
63.51(a)
685272  XM_001060927.1  XP_001060927.1 
mouse
(Mus musculus)
Taf7l5 X TAF7-like RNA polymerase II, TATA box binding protein more   -- G65785 
baker's yeast
(Saccharomyces cerevisiae)
TAF7(YMR227C)4 13(724384-722612) TFIID subunit (67 kDa), involved in RNA polymerase more   -- 855267  NP_013954.1 
About this table        Species with no ortholog for TAF7L

ENSEMBL Gene Tree for TAF7L
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for TAF7L gene
TAF72  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/105 NCBI SNPs in TAF7L are shown (see all 105 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 43)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr X posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs59513281,2
C,F,H100434589(+) CGACAG/AGAATT 1 L/P mis18Minor allele frequency- A:0.01EU EA WA 722
rs59667461,2
A,F,H100409632(+) cctgcG/Cgtcat 1 -- ng514Minor allele frequency- C:0.33EU EA WA 396
rs358996921,2
C,F100419277(+) GTGGCT/CGCTCA 1 G/S mis15Minor allele frequency- C:0.21NA EU EA WA 374
rs59919251,2
A,C,F,H100422870(+) atggcA/Gaaagg 1 -- int14Minor allele frequency- G:0.47EU EA WA 418
rs66210251,2
A,C,F,H100430646(+) actatT/Cggacc 1 -- int14Minor allele frequency- C:0.42EU EA WA 418
rs37615141,2
A,C,F,H100417860(-) AGCTCA/GAGAAG 1 -- int14Minor allele frequency- G:0.33EU EA WA 418
rs110922981,2
A,C,F,H100412624(+) ACCTAT/CATTTT 1 -- int14Minor allele frequency- C:0.33EU EA WA 418
rs110922971,2
A,C,F,H100412354(+) tGCCCT/CGTAAA 1 -- int11Minor allele frequency- C:0.29WA 110
rs37887651,2
A,C,F,H100419580(-) AAGATT/CCTTTG 1 -- int14Minor allele frequency- C:0.35EU EA WA 416
rs37887641,2
A,C,F,H100419641(-) TGTACA/GTGCTG 1 -- int112Minor allele frequency- G:0.35EU EA WA NS NA 621
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HapMap Linkage Disequilibrium images for TAF7L (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300314

Human Gene Mutation Database: TAF7L

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

9 PubMed articles for TAF7L gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 54457 HGNC: 11548 AceView: TAF7L Ensembl:ENSG00000102387 euGenes: HUgn54457
ECgene: TAF7L H-InvDB: TAF7L
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for TAF7L:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Search Novus for antibodies for TAF7L

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