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TAF7 Gene

protein-coding   GIFtS: 57

GC05M140678
TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
(Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, F, 55kD )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TAF2F)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
OTTHUMP00000160028 2
TAF(II)55 3
TAF2F 2, 3, 5
TAFII-55 3
TAFII55 1, 2, 3, 5
Descriptions
RNA polymerase II TBP-associated factor subunit F 3
TAF7 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 55 kD 2
TAF7 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 55kDa 2
TAF7 RNA polymerase II, TATA box binding protein-associated
factor, 55kDa 1
TATA box binding protein (TBP)-associated factor, RNA
polymerase II, F, 55kD 1, 2
TATA box-binding protein-associated factor 2F 2
TBP-associated factor F 2
Transcription initiation factor TFIID 55 kDa subunit 3
transcription factor IID subunit TAFII55 2
transcription initiation factor TFIID, 55 kDa subunit 2
External Ids
HGNC: 115411
Entrez Gene: 68792
UniProtKB: Q155453
Ensembl: ENSG000001789137
Search outside databases for aliases for TAF7 gene

Previous GC identifers: GC05M140514 GC05M141281 GC05M140681 GC05M140726

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for TAF7:
The intronless gene for this transcription coactivator is located between the protocadherin beta
and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the
TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits
RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID
subunit, as well as multiple transcription activators. The protein is required for transcription
by promoters targeted by RNA polymerase II. [provided by RefSeq]

UniProtKB/Swiss-Prot: TAF7_HUMAN, Q15545
Function: Functions as a component of the DNA-binding general transcription factor complex TFIID, a
multimeric protein complex that plays a central role in mediating promoter responses to various
activators and repressors. Present in both of the previously described TFIID species which either
lack or contain TAFII30 (TFIID alpha and TFIID beta respectively)

Gene Wiki entry for TAF7

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TAF7 gene  

Entrez Gene cytogenetic band: 5q31   Ensembl cytogenetic band:  5q31.3   HGNC cytogenetic band: 5q31

TAF7 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M140678:     (about GC identifiers)

Start:
140,678,239 bp from pter
End:
140,680,571 bp from pter
Size:
2,333 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000005.8  NT_029289.10  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TAF7_HUMAN, Q15545 (See protein sequence)
Recommended Name: Transcription initiation factor TFIID subunit 7  
Size: 349 amino acids; 40259 Da
Subunit: TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors
(TAFs). Interacts with TAFII250, TAFII100, TAFII28, TAFII20, and TAFII18, but not with TAFII30 or
TBP
Subcellular location: Nucleus
Secondary accessions: Q13036

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005633.2  

    ENSEMBL proteins: 
    ENSP00000312709 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (TAF7)
    Human Recombinant Proteins from Abnova (TAF7)
                  OriGene Purified Recombinant Human Protein: TAF7 

    5 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    GO:0005669 transcription factor TFIID complex IDA14580349
    GO:0005730NOT nucleolus IDA18029348
    GO:0005737 cytoplasm IDA18029348
    GO:0005794 Golgi apparatus IDA18029348
    About this table

    Antibodies for TAF7: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for TAF7
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (TAF7), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (TAF7)
    Novus Biologicals Antibodies for TAF7

    Assays for TAF7: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR006751 TAFII55_N


       GeneDecks  TAF7 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q15545

    ProtoNet protein and cluster: Q15545

    1 Blocks protein family: IPB006751 TAFII55 protein conserved region

    UniProtKB/Swiss-Prot: TAF7_HUMAN, Q15545
    Domain: The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase, which
    methylates Lys-5 in vitro (By similarity)
    Similarity: Belongs to the TAF7 family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TAF7)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TAF7)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005642

                  Applied Biosystems Silencer® siRNAs for TAF7

                  Sigma-Aldrich siRNA for TAF7  
                         Sigma-Aldrich shRNA for TAF7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for TAF7
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005642
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005642
                                     untagged cDNA clone in CMV expression vector: NM_005642 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005642

    UniProtKB/Swiss-Prot: TAF7_HUMAN, Q15545
    Function: Functions as a component of the DNA-binding general transcription factor complex TFIID, a
    multimeric protein complex that plays a central role in mediating promoter responses to various
    activators and repressors. Present in both of the previously described TFIID species which either
    lack or contain TAFII30 (TFIID alpha and TFIID beta respectively)

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003713 transcription coactivator activity TAS7824954
    GO:0005515 protein binding TAS7824954
    GO:0016251 general RNA polymerase II transcription factor activity TAS7824954
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    2 Invitrogen iPath™ Online BioAtlas - Pathways for TAF7 (Maps provided by GeneGo):
     Ligand-Dependent Transcription of Retinoid-Target genes
     Assembly of RNA Polymerase II preinitiation complex on TATA-less promoters

       GeneDecks  TAF7 for the pathways selected above  
    About GeneDecksing

    2 Millipore Pathways for TAF7
     Histone modification
     Transcription Ligand-Dependent Transcription of Retinoid-Target genes

       GeneDecks  TAF7 for the pathways selected above  
    About GeneDecksing

    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TAF7  (Your Favorite Gene powered by Ingenuity) 
     Glucocorticoid Receptor Signaling
     Estrogen Receptor Signaling

       GeneDecks  TAF7 for the pathways selected above  
    About GeneDecksing

    1 Kegg Pathway  (Kegg details for TAF7):
     hsa03022 Basal transcription factors

       GeneDecks  TAF7 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  TAF7 


    5/76 Interacting proteins for TAF7 (ENSP000003127093) via UniProtKB, MINT, and/or STRING (see all 76 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TAF1ENSP000002760723STRING (score=.999)
    TAF10ENSP000002994243STRING (score=.999)
    TAF11ENSP000003546333STRING (score=.999)
    TAF12ENSP000002639743STRING (score=.999)
    TAF4ENSP000002529963STRING (score=.999)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0006367 transcription initiation from RNA polymerase II promoter IEA--
    GO:0030520 estrogen receptor signaling pathway IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for TAF7

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TAF7)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TAF7)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005642

                  Sigma-Aldrich siRNA for TAF7  
                         Sigma-Aldrich shRNA for TAF7  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005642  

    REFSEQ mRNAs for TAF7 gene: 

    NM_005642.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005642  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005642
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005642
                                     untagged cDNA clone in CMV expression vector: NM_005642 

    Additional cDNA sequence: 

    AK314837.1 BC013397.2 BC022798.1 BC032737.1 U18062.1 X97999.1 

    16 DOTS entries:

    DT.95316543  DT.87017021  DT.91766955  DT.120808887  DT.120808871  DT.95136133  DT.100826252  DT.87017022 
    DT.120808882  DT.120808893  DT.120808895  DT.418489  DT.91766950  DT.95316545  DT.120808886  DT.95316499 

    highest scoring ESTs for TAF7:

    U18062 AA036649 AA252724 AA278660 AA312894 AA326994 AA328618 AA336858 AA369696 AA372836 

    Unigene Cluster for TAF7:

    TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
    Hs.438838  [show with all ESTs]
    Unigene Representative Sequence: NM_005642


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000313368  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    TAF7 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for TAF7

    1 / 2 / 3

    3 probe-sets matching TAF7 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    192_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 U18062 1.00 1.00 1.00 1

    201023_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005642 0.60 1.00 0.82 1

    201023_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: AAAGTGGAAA

    SOURCE GeneReport for Unigene cluster: Hs.438838

    Expression variation in blood from EXPOLDB for TAF7

    UniProtKB/Swiss-Prot: TAF7_HUMAN, Q15545
    Tissue specificity: Ubiquitous

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for TAF7 gene from 5/15 species (see all 15 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    TAF71   -- TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated more 92.74(n)
    98.85(a)
    487180  XM_544308.2  XP_544308.1 
    chimpanzee
    (Pan troglodytes)
    TAF71   -- TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated more 99.62(n)
    100(a)
    471669  XM_527047.2  XP_527047.1 
    cow
    (Bos taurus)
    TAF71   -- TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated more 91.12(n)
    97.71(a)
    509327  NM_001046028.1  NP_001039493.1 
    rat
    (Rattus norvegicus)
    Taf71   -- TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated more 89.35(n)
    96.48(a)
    307485  XM_226031.4  XP_226031.2 
    mouse
    (Mus musculus)
    Taf71, 5 18 (43.00 cM)5
    TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated more1, 5 88.66(n)1
    95.01(a)1
    240741  NM_011901.11  NP_036031.11 
     AA7634695  AC0209715  (see all 17)
    About this table        Species with no ortholog for TAF7

    ENSEMBL Gene Tree for TAF7
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for TAF7 gene
    TAF7L2  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/17 NCBI SNPs in TAF7 are shown (see all 17 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 42)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 5 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs77301,2
    A,C,F,H,O140678349(-) TTTCCT/CATGCA 1 -- ut319Minor allele frequency- C:0.33MN EU EA WA NA 918
    rs108755951,2
    A,C,F,H140680673(+) CTTGTG/TTTTCC 1 -- ng3112Minor allele frequency- N:0.00EU EA WA NA 1056
    rs103101,2
    C,F,H,O140678584(-) AATTTA/GTATTG 1 -- ut3110Minor allele frequency- G:0.18MN EU EA WA NA 2426
    rs133598201,2
    A,C,F,H140681914(+) GTACCA/GGAATG 1 -- ng314Minor allele frequency- G:0.21EU EA WA 420
    rs115476331,2
    C,F,H140680024(-) TGAATA/CAGCCT 1 -- ut518Minor allele frequency- C:0.04NA EU EA WA MN 746
    rs176027211,2
    C,F,H140679863(+) TGCAAT/CAGTTT 1 -- ut51 tfbs311Minor allele frequency- C:0.02NA EU EA WA 978
    rs18027281,2
    H140679331(-) AAGAAA/TTATAT 1 K/N mis1 trp34Minor allele frequency- T:0.00EU EA WA 418
    rs24293491,2
    C140678340(+) AACAAC/TTATTG 1 -- ut310--------
    rs10569351,2
    H140680411(-) TAATCG/AGTAGT 1 -- ut514Minor allele frequency- A:0.00EU EA WA 420
    --
    rs170971521,2
    F140678054(+) AATTGG/AAATCT 1 -- ng513Minor allele frequency- A:0.16NA 142
    About this table

    HapMap Linkage Disequilibrium images for TAF7 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 600573

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/40 PubMed articles for TAF7 gene (see all 40 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 6879 HGNC: 11541 Ensembl:ENSG00000178913 euGenes: HUgn6879 ECgene: TAF7
    H-InvDB: TAF7
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for TAF7 Genetics and Cytogenetics in Oncology and Haematology
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TAF7:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for TAF7 Antibodies for TAF7
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for TAF7 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant & Natural Proteins  

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     Recombinant Proteins
    (TAF7)
     Antibodies (TAF7)
     Tagged/untagged cDNA clones
     Validated SYBR primer pairs
     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for TAF7




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for TAF7

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