TAF6 Gene
protein-coding GIFtS : 63
GC07M099542
TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database (Previous symbol: TAF2E )
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Aliases & Descriptions for TAF6
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases DKFZp781E21155 2 MGC:8964 1 , 2 TAF(II)70 3 TAF2E 2 , 3 , 5 TAFII-70 3 TAFII-80 3 TAFII70 1 , 2 , 3 TAFII80 1 , 2 , 3 , 5 TAFII85 1 , 2
Descriptions RNA polymerase II TBP-associated factor subunit E 3 TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80 kD 2 TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa 2 TAF6 RNA polymerase II, TATA box binding protein-associated factor, 80kDa 1 TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD 1 , 2 TBP-associated factor 6 2 Transcription initiation factor TFIID 70 kDa subunit 3 transcription initiation factor TFIID 70 kD subunit 2
Search outside databases for aliases for TAF6 genePrevious GC identifers: GC07M098239 GC07M099302 GC07M099316 GC07M099351
Summaries for TAF6 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for TAF6 : Initiation of transcription by RNA polymerase II requires the activities of more than 70polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID),which binds to the core promoter to position the polymerase properly, serves as the scaffold forassembly of the remainder of the transcription complex, and acts as a channel for regulatorysignals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarilyconserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basaltranscription, serve as coactivators, function in promoter recognition or modify generaltranscription factors (GTFs) to facilitate complex assembly and transcription initiation. Thisgene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1,the largest subunit of TFIID. Four isoforms have been identified but complete transcripts havebeen determined for only three isoforms. One of the isoforms has been shown to preclude binding ofone of the other TFIID subunits, thereby reducing transcription and initiating signals thattrigger apoptosis. [provided by RefSeq] UniProtKB/Swiss-Prot: TAF6_HUMAN, P49848 Function : TAFs are components of the transcription factor IID (TFIID) complex, PCAF histoneacetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex thatplays a central role in mediating promoter responses to various activators and repressors
Gene Wiki entry for TAF6
Genomic Location for TAF6
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the TAF6 gene Entrez Gene cytogenetic band: 7q22.1 Ensembl cytogenetic band: 7q22.1 HGNC cytogenetic band: 7q TAF6 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure
GeneLoc location for GC07M099542:
(about GC identifiers )
Start:
99,542,629 bp from pter
End:
99,554,915 bp from pter
Size:
12,287 bases
Orientation:
minus strand
1 alternative location : Chr 7-,CRA_TCAG 99,064,811-99,077,098
RefSeq DNA sequence: NC_000007.12 NT_007933.14 NT_079595.2 Proteins for TAF6
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: TAF6_HUMAN, P49848 (See
protein sequence )Recommended Name: Transcription initiation factor TFIID subunit 6 Size : 677 amino acids; 72668 Da
Subunit : TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associatedfactors (TAFs). TBP is not part of TFTC. Interacts directly with TBP, TAF1/TAFII250, TAF9/TAFII31AND TAF12/TAFII20. The TAF6/TAFII70-TAF9/TAFII31 heterodimer forms an octamer complex with theTAF4B/TFII105-TAF12/TFIID20 heterodimer. Also interacts with the GTFs, TFIIEalpha/GTF2E1 andTFIIFalpha/GTF2F1. Component of the TFTC-HAT complex
Subcellular location : Nucleus
Secondary accessions : A4D2B2 A4D2B3 Q6AI29Alternative splicing : 2 isoforms : P49848-1 P49848-2
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (4 alternative transcripts):
NP_005632.1 NP_620834.1 NP_620835.1 NP_647476.1 ENSEMBL proteins: ENSP00000344537 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 5 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for TAF6: Assays for TAF6:
Protein
Domains/ Families for TAF6(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry P49848 ProtoNet protein and cluster: P49848
2 Blocks protein families : IPB004823 TATA box binding protein associated factor (TAF) IPB011442 Protein of unknown function DUF1546 UniProtKB/Swiss-Prot: TAF6_HUMAN, P49848 Similarity : Belongs to the TAF6 family
Gene Function for TAF6
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4 ): NM_005641 Applied Biosystems Silencer ® siRNAs for TAF6 Sigma-Aldrich siRNA for TAF6 Sigma-Aldrich shRNA for TAF6 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 4 ): NM_005641                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 4 ): NM_005641                                  untagged cDNA clones in CMV expression vector (see all 4 ): NM_005641  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_005641 UniProtKB/Swiss-Prot: TAF6_HUMAN, P49848 Function : TAFs are components of the transcription factor IID (TFIID) complex, PCAF histoneacetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex thatplays a central role in mediating promoter responses to various activators and repressors
4 Gene Ontology (GO) molecular function terms (links to tree view) :
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Pathways & Interactions for TAF6
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for TAF6 (Your Favorite Gene powered by Ingenuity) Gene Network CentralTM Interacting Genes and Proteins Network for TAF6 5/91 Interacting proteins for TAF6 (P49848 2 ENSP00000344537 3 ) via UniProtKB, MINT, and/or STRING (see all 91
)About this table 5 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0006350 transcription
IEA -- GO:0006355 regulation of transcription, DNA-dependent
IEA -- GO:0006367 transcription initiation from RNA polymerase II promoter
EXP 8946909 GO:0006368 RNA elongation from RNA polymerase II promoter
EXP 9405375 GO:0051090 regulation of transcription factor activity
IEA --
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Drugs & Compounds for TAF6 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for TAF6
Transcripts for TAF6(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
Sigma-Aldrich ,
OriGene ,
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4 ): NM_005641 Sigma-Aldrich siRNA for TAF6 Sigma-Aldrich shRNA for TAF6 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_005641 NM_139122 NM_139123 NM_139315
REFSEQ mRNAs for TAF6 gene (4 alternative transcripts): NM_005641.2 NM_139122.1 NM_139123.1 NM_139315.1
Applied Biosystems TaqMan ® Gene Expression Assays: NM_005641 NM_139122 NM_139123 NM_139315
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 4 ): NM_005641                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 4 ): NM_005641                                  untagged cDNA clones in CMV expression vector (see all 4 ): NM_005641  
Additional cDNA sequence: AK097781.1 AK300005.1 AK314428.1 AK315077.1 BC018115.1 BC127819.1 CR624352.1 CR627390.1 L25444.1 U31659.1
24/28 DOTS entries (see all 28
): DT.450124 DT.100723809 DT.92468297 DT.100044202 DT.100645712 DT.100723786 DT.100767792 DT.100059866 DT.100723768 DT.100723802 DT.100723777 DT.97774211 DT.100059865 DT.100723773 DT.40255906 DT.95273993 DT.100876224 DT.121080631 DT.92468307 DT.100051569 DT.121080706 DT.92468322 DT.92468328 DT.95273992
24/175 AceView cDNA sequences (see all 175
):F01487 AU124861 AK097781 CF243183 BM806299 F05233 NM_005641 BM557210 BU169243 NM_139122 BQ223742 BU184274 BM458212 NM_139315 NM_139123 BG258813 BX396535 BQ073263 CR624352 BQ962884 BQ072075 BU508273 BM553684 BQ440506
highest scoring ESTs for TAF6 :L25444 AK097781 AU124863 AU131945 AU143362 BC018115 BE383825 BE384921 BG337701 BG832031
Unigene Cluster for TAF6: TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa Hs.489309 [show with all ESTs ] Unigene Representative Sequence: NM_005641 GeneLoc Exon Structure 5/20 Alternative Splicing Database (ASD) splice patterns (SP) for TAF6 (see all 20
) ExUns: 1 ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b · 7c · 7d ^ 8 ^ 9a · 9b · 9c · 9d · 9e ^ 10a · 10b ^ 11a · 11b · 11c · SP1 :                                           -           SP2 :               -   -   -   -   -   -                   -           SP3 :               -   -   -   -   -                     -           SP4 :               -   -     -   -                     -           SP5 :               -   -   -   -   -                     -   -        
ExUns: 11d ^ 12 ^ 13 ^ 14 ^ 15 SP1 :           SP2 :           SP3 :           SP4 :           SP5 :          
About this scheme ECgene alternative splicing isoforms for TAF6 1 Ensembl transcript including schematic representation : ENST00000344095
Expression for TAF6
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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TAF6 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for TAF6 1 / 2 / 3
3 probe-sets matching TAF6 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: TCACTTTCTTSOURCE GeneReport for Unigene cluster: Hs.489309 Expression variation in blood from EXPOLDB for TAF6
Orthologs for TAF6
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for TAF6 gene from 5/18 species (see all 18
)
About this table Species with no ortholog for TAF6 ENSEMBL Gene Tree for TAF6 Paralogs for TAF6 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for TAF6 gene TAF6L 2
SNPs/Variants for TAF6 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for TAF6 (up to first 250kb)
Disorders & Mutations for TAF6
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 602955
Medical News for TAF6 (Possibly Related Articles in
Doctor's Guide )
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Publications for TAF6 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/50 PubMed articles for TAF6 gene (see all 50
): Evolutionary conservation of human TATA-binding-polypeptide- associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors. (PubMed id 7667268) 1, 3, 4 Hisatake K.... Roeder R.G. (1995) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243) 3, 4 Beausoleil S.A.... Gygi S.P. (2006) Core promoter binding by histone-like TAF complexes. (PubMed id 15601843) 3, 4 Shao H.... Dikstein R. (2005) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. (PubMed id 15302935) 3, 4 Beausoleil S.A....Gygi S.P. (2004) Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis. (PubMed id 12601814) 3, 4 Cavusoglu N....Van Dorsselaer A. (2003) Human chromosome 7: DNA sequence and biology. (PubMed id 12690205) 3, 4 Scherer S.W....Tsui L.C. (2003) Cloning and expression of Drosophila TAFII60 and human TAFII70 reveal conserved interactions with other subunits of TFIID. (PubMed id 8262073) 3, 4 Weinzierl R.O.... Tjian R. (1993) Discussion paper: experimental and clinical immunopharmacology data applicable to cancer immunotherapy. (PubMed id 826207) 2, 3 Mathe G....Simmler M.C. (1976) TAFII70 isoform-specific growth suppression correlates with its ability to complex with the GADD45a protein. (PubMed id 15328371) 1, 3 Wang W....Marks J.R. (2004)
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AND OR
Aliases
TAF6 (Gene Symbol) DKFZp781E21155 MGC:8964 TAF(II)70 TAF2E TAFII-70 TAFII-80 TAFII70 TAFII80 TAFII85 RNA polymerase II TBP-associated factor subunit E TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80 kD TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa TAF6 RNA polymerase II, TATA box binding protein-associated factor, 80kDa TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD TBP-associated factor 6 Transcription initiation factor TFIID 70 kDa subunit transcription initiation factor TFIID 70 kD subunit
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Query String
PubMed
OMIM
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Genome Databases showing TAF6
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing TAF6
(According to HUGE )
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Specialized Databases showing TAF6 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Name Description
NIEHS-SNPs http://egp.gs.washington.edu/data/taf6/
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-- Services for TAF6 (Reagents available from Applied Biosystems , Antibodies and assays by Cell
Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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Products for TAF6:
Recombinant Proteins (TAF6 ) Antibodies (TAF6 )
Search Tocris compounds for TAF6
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Aliases for TAF6
Databases for TAF6
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Domains/Families for TAF6
Drugs/Compounds for TAF6
Expression for TAF6
Function for TAF6
Location for TAF6
Medical News for TAF6
Orthologs for TAF6
Paralogs for TAF6
Pathways/Interactions for TAF6
Proteins for TAF6
Publications for TAF6
SNPs for TAF6
Search Box for TAF6
Services for TAF6
Summaries for TAF6
Technologies for TAF6
Transcripts for TAF6
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GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009