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TAF5L Gene

protein-coding   GIFtS: 59

GC01M227795
TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
(Previous names: TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65 kD )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
OTTHUMP00000037470 2
OTTHUMP00000037471 2
PAF65-beta 3
PAF65B 1, 2, 3
Descriptions
PCAF associated factor 65 beta 2
PCAF-associated factor 65 beta 3
TAF5-like RNA polymerase II, p300/CBP-associated factor
(PCAF)-associated factor, 65 kD 1, 2
TAF5-like RNA polymerase II, p300/CBP-associated factor
(PCAF)-associated factor, 65kDa 2
TAF5-like RNA polymerase II, p300/CBP-associated
factor-associated factor, 65kDa 1
External Ids
HGNC: 173041
Entrez Gene: 270972
UniProtKB: O755293
Ensembl: ENSG000001358017
Search outside databases for aliases for TAF5L gene

Previous GC identifers: GC01M228175 GC01M225423 GC01M226128 GC01M226690 GC01M226035

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for TAF5L:
The product of this gene belongs to the WD-repeat TAF5 family of proteins. This gene encodes a
protein that is a component of the PCAF histone acetylase complex. The PCAF histone acetylase
complex, which is composed of more than 20 polypeptides some of which are TAFs, is required for
myogenic transcription and differentiation. TAFs may participate in basal transcription, serve as
coactivators, function in promoter recognition or modify general transcription factors to
facilitate complex assembly and transcription initiation. The encoded protein is structurally
similar to one of the histone-like TAFs, TAF5. Alternatively spliced transcript variants encoding
different isoforms have been identified for this gene. [provided by RefSeq]

UniProtKB/Swiss-Prot: TAF5L_HUMAN, O75529
Function: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently
acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human
version of the yeast SAGA complex

Gene Wiki entry for TAF5L

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TAF5L gene  

Entrez Gene cytogenetic band: 1q42.13   Ensembl cytogenetic band:  1q42.13   HGNC cytogenetic band: 1q42.11-q42.3

TAF5L Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M227795:     (about GC identifiers)

Start:
227,795,481 bp from pter
End:
227,828,417 bp from pter
Size:
32,937 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_004559.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TAF5L_HUMAN, O75529 (See protein sequence)
Recommended Name: TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa
subunit 5L  
Size: 589 amino acids; 66155 Da
Subunit: The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5,
TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as
TADA2L/ADA2, TADA3L/ADA3 and SPT3. Component of the STAGA transcription coactivator-HAT complex,
at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and
TAF9
Subcellular location: Nucleus
Secondary accessions: Q5TDI6 Q8IW31
Alternative splicing: 2 isoforms:  O75529-1   O75529-2   

REFSEQ proteins (2 alternative transcripts): 
NP_001020418.1  NP_055224.1  


ENSEMBL proteins: 
ENSP00000258281 ENSP00000355636 ENSP00000355635 ENSP00000355634 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
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Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (TAF5L)
Human Recombinant Proteins from Abnova (TAF5L)
              OriGene Purified Recombinant Human Protein: TAF5L 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
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Antibodies for TAF5L: 
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Sigma-Aldrich Antibodies for TAF5L
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Antibodies from Abcam (TAF5L), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (TAF5L)
Novus Biologicals Antibodies for TAF5L

Assays for TAF5L: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5/7 InterPro domains/families (see all 7 ):
 IPR019782 WD40_repeat_2
 IPR019775 WD40_repeat_CS
 IPR001680 WD40_repeat
 IPR017986 WD40_repeat_region
 IPR015943 WD40/YVTN_repeat-like


   GeneDecks  TAF5L for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry O75529

ProtoNet protein and cluster: O75529

2 Blocks protein families:
IPB001680 G-protein beta WD-40 repeat signature
IPB007582 WD40 associated region in TFIID subunit


UniProtKB/Swiss-Prot: TAF5L_HUMAN, O75529
Similarity: Belongs to the WD repeat TAF5 family
Similarity: Contains 6 WD repeats

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TAF5L)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TAF5L)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_014409

              Applied Biosystems Silencer® siRNAs for TAF5L

              Sigma-Aldrich siRNA for TAF5L  
                     Sigma-Aldrich shRNA for TAF5L  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for TAF5L
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001025247
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001025247
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001025247 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014409

UniProtKB/Swiss-Prot: TAF5L_HUMAN, O75529
Function: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently
acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human
version of the yeast SAGA complex

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003700 transcription factor activity TAS9674425
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

2 Invitrogen iPath™ Online BioAtlas - Pathways for TAF5L (Maps provided by GeneGo):
 Ligand-Dependent Transcription of Retinoid-Target genes
 Estrogen receptor signaling pathway

   GeneDecks  TAF5L for the pathways selected above  
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2 Millipore Pathways for TAF5L
 Histone modification
 Transcription Ligand-Dependent Transcription of Retinoid-Target genes

   GeneDecks  TAF5L for the pathways selected above  
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TAF5L  (Your Favorite Gene powered by Ingenuity) 
 Glucocorticoid Receptor Signaling
 Estrogen Receptor Signaling

   GeneDecks  TAF5L for the pathways selected above  
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1 Kegg Pathway  (Kegg details for TAF5L):
 hsa03022 Basal transcription factors

   GeneDecks  TAF5L for the pathways selected above  
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3 Interacting proteins for TAF5L (ENSP000003556363) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
TAF5ENSP000003588543STRING (score=.81)
KAT2BENSP000002637543STRING (score=.804)
C1orf173ENSP000003420143STRING (score=.7)
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2 Gene Ontology (GO) biological process terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0006366 transcription from RNA polymerase II promoter TAS9674425
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for TAF5L

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (TAF5L)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (TAF5L)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_014409

              Sigma-Aldrich siRNA for TAF5L  
                     Sigma-Aldrich shRNA for TAF5L  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001025247  NM_014409  

REFSEQ mRNAs for TAF5L gene (2 alternative transcripts): 

NM_001025247.1   NM_014409.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001025247  NM_014409  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001025247
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001025247
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001025247 

Additional cDNA sequence: 

AF069736.1 AJ009770.1 BC001569.1 BC041094.1 

5 DOTS entries:

DT.85102616  DT.434299  DT.91830511  DT.97817838  DT.91992393 

24/98 AceView cDNA sequences (see all 98 ):

AA055381 AA055932 BE646579 AL041448 AV656444 AJ009770 D31141 AI356285 
AA902634 AA524094 AI590283 AV660159 NM_014409 AA913241 BF431473 BC001569 
AI479619 BM090850 AI251746 AI475890 AI075086 AA302683 AA557305 AW070931 

highest scoring ESTs for TAF5L:

AJ009770 AW105033 BC041094 BE646579 BM559120 AA055381 AA055932 AA282026 AA282682 AA302683 

Unigene Cluster for TAF5L:

TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
Hs.270621  [show with all ESTs]
Unigene Representative Sequence: NM_001025247


GeneLoc Exon Structure

4 Ensembl transcripts including schematic representations:
ENST00000258281  ENST00000366676  ENST00000366675  ENST00000366674  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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TAF5L expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for TAF5L

1 / 2 / 3

5 probe-sets matching TAF5L gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
35186_at2, 3 U95-A 1 1.00 1.00 0.23 1.44 AJ009770 0.80 1.00 0.91 1

85119_r_at2, 3 U95-E 1 1.00 1.00 0.93 0.60 AI075086 0.60 1.00 0.82 1

213654_at2, 3 U133-A 1 1.00 1.00 -- -- R60550 0.60 1.00 0.82 1

213654_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

1554415_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  TAF5L for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: GATGAACTTG

SOURCE GeneReport for Unigene cluster: Hs.270621

Expression variation in blood from EXPOLDB for TAF5L

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for TAF5L gene from 5/9 species (see all 9 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TAF5L1   -- TAF5-like RNA polymerase II, p300/CBP-associated factor more 93.38(n)
98.98(a)
488982  XM_546100.2  XP_546100.2 
chimpanzee
(Pan troglodytes)
TAF5L1   -- TAF5-like RNA polymerase II, p300/CBP-associated factor more 99.6(n)
100(a)
457807  XM_514261.2  XP_514261.2 
rat
(Rattus norvegicus)
Taf5l1   -- TAF5-like RNA polymerase II, p300/CBP-associated factor more 86.47(n)
93.55(a)
307927  XM_226577.4  XP_226577.3 
mouse
(Mus musculus)
Taf5l1, 5 85
TAF5-like RNA polymerase II, p300/CBP-associated factor more1, 5 86.7(n)1
93.38(a)1
1021621  NM_133966.21  NP_598727.11 
 AA6840325  AI8490205  (see all 10)
chicken
(Gallus gallus)
TAF5L1   -- TAF5-like RNA polymerase II, p300/CBP-associated factor more 82(n)
92.53(a)
421538  XM_419579.2  XP_419579.2 
About this table        Species with no ortholog for TAF5L

ENSEMBL Gene Tree for TAF5L
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for TAF5L gene
TAF52  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/175 NCBI SNPs in TAF5L are shown (see all 175 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 80)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 1 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs18168781,2
A,C,F,H,O227804303(+) TTAGGC/TTTAGT 2 -- ut31 int1 ese312Minor allele frequency- T:0.27NS EU EA WA NA 650
--
rs38203591,2
C,F227803850(+) TCTAAG/AGTAGT 2 -- int1 ut313Minor allele frequency- A:0.33NA 138
--
rs168601221,2
F227804471(+) GAAACT/CAGGAA 2 -- ut31 int1 ese33Minor allele frequency- C:0.02NA 134
rs37538861,2
A,C,F,H227804793(+) TTGACG/TCGCTT 2 R/R syn1 ese313Minor allele frequency- T:0.25EA NA EU WA 2538
rs49255001,2
A,C,F227804859(+) ATGTCA/GGGGGG 2 P/P syn1 ese31Minor allele frequency- G:0.13NA 76
rs22956251,2
C,F,H227797075(+) TGAGCG/ACTCCA 1 S/S syn110Minor allele frequency- A:0.33EA EU WA NA 2202
rs102184321,2
C227797417(+) CAGTGG/ACCCCG 1 G/G syn1 ese31Minor allele frequency- A:0.01NA 74
rs350154251,2
F227797405(+) TACACT/CGGTCC 1 P/P syn11Minor allele frequency- C:0.03NA 74
rs22735731,2
A,C,F,H,O227812642(+) CAGTAT/CGTGGC 2 -- int112Minor allele frequency- C:0.24EA EU WA NA 2390
rs75476071,2
A,C,F,H227816659(+) ATTGAC/TGTGAG 2 -- int1 ese38Minor allele frequency- T:0.16EU EA WA 832
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HapMap Linkage Disequilibrium images for TAF5L (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

Genetic Association Database: TAF5L
Human Genome Epidemiology Navigator: TAF5L (2 documents)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/16 PubMed articles for TAF5L gene (see all 16 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 27097 HGNC: 17304 AceView: TAF5L Ensembl:ENSG00000135801 euGenes: HUgn27097
ECgene: TAF5L H-InvDB: TAF5L
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for TAF5L:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for TAF5L Antibodies for TAF5L
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for TAF5L Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
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 Antibodies for TAF5L

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