TAF5 Gene
protein-coding GIFtS : 59
GC10P105117
TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database (Previous symbol: TAF2D )
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Aliases & Descriptions for TAF5
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases OTTHUMP00000020402 2 TAF(II)100 3 TAF2D 2 , 3 , 5 TAFII-100 3 TAFII100 1 , 2 , 3 , 5
Descriptions TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa 2 TAF5 RNA polymerase II, TATA box binding protein-associated factor, 100kDa 1 TATA box binding protein (TBP)-associated factor 2D 2 TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD 1 , 2 TBP-associated factor 5 2 Transcription initiation factor TFIID 100 kDa subunit 3 transcription initiation factor TFIID 100 kD subunit 2
Search outside databases for aliases for TAF5 genePrevious GC identifers: GC10P104020 GC10P104361 GC10P105258 GC10P104792
Summaries for TAF5 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for TAF5 : Initiation of transcription by RNA polymerase II requires the activities of more than 70polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID),which binds to the core promoter to position the polymerase properly, serves as the scaffold forassembly of the remainder of the transcription complex, and acts as a channel for regulatorysignals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarilyconserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basaltranscription, serve as coactivators, function in promoter recognition or modify generaltranscription factors (GTFs) to facilitate complex assembly and transcription initiation. Thisgene encodes an integral subunit of TFIID associated with all transcriptionally competent forms ofthat complex. This subunit interacts strongly with two TFIID subunits that show similarity tohistones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex.[provided by RefSeq] UniProtKB/Swiss-Prot: TAF5_HUMAN, Q15542 Function : TAFs are components of the transcription factor IID (TFIID) complex, PCAF histoneacetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential formediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with thehistone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stablecomplex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions ofTAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55,also have been observed
Gene Wiki entry for TAF5
Genomic Location for TAF5
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the TAF5 gene Entrez Gene cytogenetic band: 10q24-q25.2 Ensembl cytogenetic band: 10q24.33 HGNC cytogenetic band: 10q24-q25.2 TAF5 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 10 GeneLoc Exon Structure
GeneLoc location for GC10P105117:
(about GC identifiers )
Start:
105,117,714 bp from pter
End:
105,138,812 bp from pter
Size:
21,099 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000010.9 NT_030059.12 Proteins for TAF5
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: TAF5_HUMAN, Q15542 (See
protein sequence )Recommended Name: Transcription initiation factor TFIID subunit 5 Size : 800 amino acids; 86830 Da
Subunit : TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associatedfactors (TAFs). TBP is not part of TFTC. Component of the TFTC-HAT complex, at least composed ofTAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10and TRRAP. Interacts with SV40 Large T antigen
Subcellular location : Nucleus
PDB structures from and Proteopedia : 2NXP (3D)
 
Secondary accessions : B2RMR0 Q53EM4 Q5SYD5 Q86UZ7 Q9Y4K5Alternative splicing : 2 isoforms : Q15542-1 Q15542-2
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins: NP_008882.2 ENSEMBL proteins: ENSP00000311024 ENSP00000358854 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6
):
About this table Antibodies for TAF5: Assays for TAF5:
Protein
Domains/ Families for TAF5(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q15542 ProtoNet protein and cluster: Q15542
3 Blocks protein families : IPB001680 G-protein beta WD-40 repeat signature IPB006594 Lissencephaly type-1-like homology motif IPB007582 WD40 associated region in TFIID subunit UniProtKB/Swiss-Prot: TAF5_HUMAN, Q15542 Domain : Distinct domains of TAF5/TAFII100 are required for functional interaction withtranscription factor TFIIFB (RAP30) and incorporation into the TFIID complex Similarity : Belongs to the WD repeat TAF5 familySimilarity : Contains 1 LisH domainSimilarity : Contains 6 WD repeats
Gene Function for TAF5
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
OriGene ,
Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
from Invitrogen ,
Millipore ,
OriGene , and/or
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siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_006951 Applied Biosystems Silencer ® siRNAs for TAF5 Sigma-Aldrich siRNA for TAF5 Sigma-Aldrich shRNA for TAF5 Explore Sigma-Aldrich super-pooled esiRNAs               OriGene GFP tagged cDNA clone in CMV expression vector: NM_006951                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_006951                                  untagged cDNA clone in CMV expression vector: NM_006951  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_006951 UniProtKB/Swiss-Prot: TAF5_HUMAN, Q15542 Function : TAFs are components of the transcription factor IID (TFIID) complex, PCAF histoneacetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential formediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with thehistone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stablecomplex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions ofTAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55,also have been observed
3 Gene Ontology (GO) molecular function terms (links to tree view) :
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Pathways & Interactions for TAF5
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for TAF5 (Your Favorite Gene powered by Ingenuity) Gene Network CentralTM Interacting Genes and Proteins Network for TAF5 5/86 Interacting proteins for TAF5 (ENSP00000358854 3 ) via UniProtKB, MINT, and/or STRING (see all 86
)About this table 4 Gene Ontology (GO) biological process terms (links to tree view) :
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Drugs & Compounds for TAF5 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for TAF5
Transcripts for TAF5(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
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OriGene ,
Tagged/untagged cDNA clones from
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_006951 Sigma-Aldrich siRNA for TAF5 Sigma-Aldrich shRNA for TAF5 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_006951
REFSEQ mRNAs for TAF5 gene: NM_006951.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_006951
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_006951                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_006951                                  untagged cDNA clone in CMV expression vector: NM_006951  
Additional cDNA sequence: AK223615.1 AK291229.1 AL137373.1 BC052268.1 BC136340.1 BC136348.1 CR610839.1 U75309.1 U80191.1 X95525.1
3 DOTS entries : DT.121272508 DT.442101 DT.121272568
24/41 AceView cDNA sequences (see all 41
):U75309 U80191 AL524839 BC052268 BF056948 CR610839 AA977222 AA322411 BX113246 NM_006951 AA251738 AA827897 AA761844 AW241461 BE503231 X95525 BG290312 AI452991 BE778147 AL515577 BG533689 BU193699 AI590915 AI269393
highest scoring ESTs for TAF5 :U75309 X95525 AA251738 AA329391 AA761844 AA827897 AA977222 AI269393 AI452991 AI470331
Unigene Cluster for TAF5: TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa Hs.96103 [show with all ESTs ] Unigene Representative Sequence: NM_006951 GeneLoc Exon Structure 2 Ensembl transcripts including schematic representations : ENST00000351396
ENST00000369839
Expression for TAF5
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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TAF5 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for TAF5 1 / 2 / 3
5 probe-sets matching TAF5 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: TTCTACTTCASOURCE GeneReport for Unigene cluster: Hs.96103 Expression variation in blood from EXPOLDB for TAF5
Orthologs for TAF5
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for TAF5 gene from 5/22 species (see all 22
)
About this table Species with no ortholog for TAF5 ENSEMBL Gene Tree for TAF5 Paralogs for TAF5 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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Paralogs for TAF5 gene TAF5L 2
SNPs/Variants for TAF5 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for TAF5 (up to first 250kb)
Disorders & Mutations for TAF5
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 601787
Medical News for TAF5 (Possibly Related Articles in
Doctor's Guide )
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--
Publications for TAF5 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/41 PubMed articles for TAF5 gene (see all 41
): Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100. (PubMed id 8942982) 2, 3, 4 Tanese N....Admon A. (1996) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis. (PubMed id 12601814) 3, 4 Cavusoglu N....Van Dorsselaer A. (2003) Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction. (PubMed id 10373431) 3, 4 Brand M.... Tora L. (1999) Specific interactions and potential functions of human TAFII100. (PubMed id 9045704) 3, 4 Tao Y.... Roeder R.G. (1997) Distinct domains of hTAFII100 are required for functional interaction with transcription factor TFIIF beta (RAP30) and incorporation into the TFIID complex. (PubMed id 8758937) 3, 4 Dubrovskaya V....Tora L. (1996) Localization of the gene (TAF2D) encoding the 100-kDa subunit (hTAFII100) of the human TFIID complex to chromosome 10 band q24-q25.2. (PubMed id 8884287) 2, 3 Dubrovskaya V....Tora L. (1996) Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348) 3 Barbe L....Andersson-Svahn H. (2008) Structural analysis and dimerization potential of the human TAF5 subunit of TFIID. (PubMed id 17227857) 3 Bhattacharya S....Jacobson R.H. (2007) SUMO-1 modification of human transcription factor (TF) IID complex subunits: inhibition of TFIID promoter-binding activity through SUMO-1 modification of hsTAF5. (PubMed id 15637059) 3 Boyer-Guittaut M....Oelgeschlager T. (2005)
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Genome Databases showing TAF5
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing TAF5
(According to HUGE )
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Specialized Databases showing TAF5 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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