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TAF4B Gene

protein-coding   GIFtS: 54

GC18P022039
TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa
(Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, C2, 105kD )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: TAF2C2)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
TAF2C2 2, 3, 5
TAFII-105 3
TAFII105 1, 2, 3, 5
Descriptions
TAF4b RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 105kDa 2
TAF4b RNA polymerase II, TATA box binding
protein-associated factor, 105kDa 1
TATA box binding protein (TBP)-associated factor 4B 2
TATA box binding protein (TBP)-associated factor, RNA
polymerase II, C2, 105kD 1, 2
Transcription initiation factor TFIID 105 kDa subunit 3
transcription initiation factor TFIID 105 kD subunit 2
External Ids
HGNC: 115381
Entrez Gene: 68752
UniProtKB: Q927503
Ensembl: ENSG000001413847
Search outside databases for aliases for TAF4B gene

Previous GC identifers: GC18P023722 GC18P023538 GC18P022037

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for TAF4B:
TATA-binding protein associated factors (TAFs) participate, with TATA binding protein (TBP; MIM
600075), in the formation of the TFIID protein complex (see MIM 313650), which is involved in the
initiation of gene transcription by RNA polymerase II (see MIM 180660).[supplied by OMIM]

UniProtKB/Swiss-Prot: TAF4B_HUMAN, Q92750
Function: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in
certain cells. TFIID is a multimeric protein complex that plays a central role in mediating
promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator
of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes
including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with
OCBA/POU2AF1, acts as a coactivator of B cell-specific transcription

Gene Wiki entry for TAF4B

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TAF4B gene  

Entrez Gene cytogenetic band: 18q11.2   Ensembl cytogenetic band:  18q11.2   HGNC cytogenetic band: 18q11.1

TAF4B Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 18         GeneLoc Exon Structure

GeneLoc location for GC18P022039:     (about GC identifiers)

Start:
22,060,407 bp from pter
End:
22,225,647 bp from pter
Size:
165,241 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000018.8  NT_010966.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: TAF4B_HUMAN, Q92750 (See protein sequence)
Recommended Name: Transcription initiation factor TFIID subunit 4B  
Size: 862 amino acids; 91091 Da
Subunit: TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors
(TAFs). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain. This
heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer. Interacts
with P65/RELA homodimers and P65/RELA-REL heterodimers. Interaction with POU2AF1, via its
C-terminal activation domain, is required for octamer-dependent transcription
Subcellular location: Nucleus. Cytoplasm. Note=Export into the cytoplasm is mediated by a
CRM1-independent nuclear export pathway and not by phosphorylation
Secondary accessions: Q29YA4 Q29YA5
Alternative splicing: 2 isoforms:  Q92750-1   Q92750-2   

Post-translational modifications:

  • Under stimulation by forskolin, Isoform 1 is phosphorylated by protein kinase A (PKA)1


  • REFSEQ proteins: NP_005631.1  

    ENSEMBL proteins: 
    ENSP00000383314 ENSP00000269142 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (TAF4B)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    4 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus NAS--
    GO:0005654 nucleoplasm EXP1939271 2449431 8946909
    GO:0005669 transcription factor TFIID complex IEA--
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for TAF4B: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (TAF4B), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for TAF4B

    Assays for TAF4B: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR007900 TAF4
     IPR003894 TAFH_NHR1
     IPR009072 Histone-fold


       GeneDecks  TAF4B for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q92750

    ProtoNet protein and cluster: Q92750

    2 Blocks protein families:
    IPB003894 TAF-like region
    IPB007900 Transcription initiation factor TFIID component TAF4


    UniProtKB/Swiss-Prot: TAF4B_HUMAN, Q92750
    Similarity: Belongs to the TAF4 family
    Similarity: Contains 1 histone-fold domain
    Similarity: Contains 1 TAFH (NHR1) domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TAF4B)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_005640

                  Applied Biosystems Silencer® siRNAs for TAF4B

                  Sigma-Aldrich siRNA for TAF4B  
                         Sigma-Aldrich shRNA for TAF4B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005640
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005640
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_005640 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005640

    UniProtKB/Swiss-Prot: TAF4B_HUMAN, Q92750
    Function: Cell type-specific subunit of TFIID that may function as a gene-selective coactivator in
    certain cells. TFIID is a multimeric protein complex that plays a central role in mediating
    promoter responses to various activators and repressors. TAFII105 is a transcriptional coactivator
    of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes
    including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with
    OCBA/POU2AF1, acts as a coactivator of B cell-specific transcription

    2 MGI mutant phenotypes (inferred from 1 allele(MGI details for Taf4b):

    endocrine/exocrine glandreproductive system

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700 transcription factor activity IEA--
    GO:0016986 transcription initiation factor activity NAS--
    GO:0051059 NF-kappaB binding IPI10849440
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for TAF4B
     Transcription Ligand-Dependent Transcription of Retinoid-Target genes

       GeneDecks  TAF4B for the pathways selected above  
    About GeneDecksing

    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TAF4B  (Your Favorite Gene powered by Ingenuity) 
     Estrogen Receptor Signaling
     Glucocorticoid Receptor Signaling

       GeneDecks  TAF4B for the pathways selected above  
    About GeneDecksing

    2 Kegg Pathways  (Kegg details for TAF4B):
     hsa03022 Basal transcription factors
     hsa05016 Huntington's disease

       GeneDecks  TAF4B for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  TAF4B 


    5/49 Interacting proteins for TAF4B (ENSP000002691423) via UniProtKB, MINT, and/or STRING (see all 49 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TBPENSP000002303543STRING (score=.989)
    TAF12ENSP000002639743STRING (score=.986)
    TAF5ENSP000003588543STRING (score=.985)
    GTF2A1ENSP000002981733STRING (score=.978)
    TAF6ENSP000003445373STRING (score=.967)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent NAS--
    GO:0006367 transcription initiation from RNA polymerase II promoter EXP8946909
    GO:0006368 RNA elongation from RNA polymerase II promoter EXP9405375
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for TAF4B

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TAF4B)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_005640

                  Sigma-Aldrich siRNA for TAF4B  
                         Sigma-Aldrich shRNA for TAF4B  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005640  

    REFSEQ mRNAs for TAF4B gene: 

    NM_005640.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005640  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005640
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005640
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_005640 

    Additional cDNA sequence: 

    AB234096.1 AK302593.1 AY888048.1 BC016360.1 BC032791.1 Y09321.1 

    3 DOTS entries:

    DT.442127  DT.101966187  DT.92420195 

    24/41 AceView cDNA sequences (see all 41 ):

    AA287145 BC016360 AA412656 AA458814 AA721195 BX952140 BX952116 AA287057 
    W77893 BC032791 BM973282 R24013 AA825151 AL121204 AA976985 BM853763 
    W72761 BQ012407 BG213757 Y09321 AA252510 AA976562 BG217524 AA252384 

    highest scoring ESTs for TAF4B:

    Y09321 AA252384 AA252510 AA287145 AA412656 AA458814 AA825151 AA976562 AA976985 AL079947 

    Unigene Cluster for TAF4B:

    TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa
    Hs.369519  [show with all ESTs]
    Unigene Representative Sequence: NM_005640


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000400466  ENST00000269142  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    TAF4B expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for TAF4B

    1 / 2 / 3

    6 probe-sets matching TAF4B gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    45663_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AL079947 0.80 1.00 0.91 1

    34628_at2, 3 U95-A 2 1.00 0.75 0.66 0.23 Y09321 1.00 1.00 1.00 1

    235020_at2, 3 U133-B 1 1.00 1.00 -- -- AI366784 0.60 1.00 0.82 1

    216226_at2, 3 U133-A 2 1.00 0.82 -- -- Y09321 1.00 1.00 1.00 1

    235020_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    216226_at2 U133Plus2 2 1.00 0.82 -- -- -- -- -- -- --
    GeneDecks  TAF4B for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TTGGCACATT

    SOURCE GeneReport for Unigene cluster: Hs.369519

    Expression variation in blood from EXPOLDB for TAF4B

    UniProtKB/Swiss-Prot: TAF4B_HUMAN, Q92750
    Tissue specificity: Preferentially expressed in ovarian granulosa cells (at protein level). Highly
    expressed in B cells

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for TAF4B gene from 5/8 species (see all 8 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    TAF4B1   -- TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated more 90.16(n)
    88.49(a)
    490507  XM_547629.2  XP_547629.2 
    chimpanzee
    (Pan troglodytes)
    TAF4B1   -- TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated more 86.9(n)
    84.52(a)
    455347  XM_512071.2  XP_512071.2 
    cow
    (Bos taurus)
    TAF4B1   -- TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated more 87.5(n)
    86.64(a)
    518030  XM_596212.3  XP_596212.3 
    rat
    (Rattus norvegicus)
    Taf4b1   -- TAF4B RNA polymerase II, TATA box binding protein (TBP)-associated more 84.01(n)
    76.77(a)
    680806  XM_001058968.1  XP_001058968.1 
    mouse
    (Mus musculus)
    Taf4b1, 5 18 (43.00 cM)5
    TAF4B RNA polymerase II, TATA box binding protein (TBP)-associated more1, 5 85.02(n)1
    79.81(a)1
    725041  XM_128905.61  XP_128905.41 
     AA5490395  AA7268005  (see all 13)
    About this table        Species with no ortholog for TAF4B

    ENSEMBL Gene Tree for TAF4B
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for TAF4B gene
    TAF42  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/860 NCBI SNPs in TAF4B are shown (see all 860 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 229)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 18 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs15425331,2
    A,C,F,H,O22224578(+) ATGCCA/GTTTCT 1 -- ut3120Minor allele frequency- G:0.34MN EU EA WA NA 3316
    rs138551,2
    A,C,F,H22224715(+) TGAGGG/AGAATG 1 -- ut318Minor allele frequency- A:0.06MN EU EA WA NA 712
    rs88201,2
    A,C,F,H22224945(+) TTATAT/GTTCCT 1 -- ut317Minor allele frequency- G:0.31MN EA EU WA 2278
    rs11159791,2
    A,C,F,H22225774(+) CATTTT/CGGTTC 1 -- ng3111Minor allele frequency- C:0.48EU EA WA NA 978
    rs169422191,2
    C,F,H22108772(+) TTCAGA/GTTAAT 1 I/V mis111Minor allele frequency- G:0.02NA EU EA WA 966
    rs19712471,2
    C,F22224675(+) AAGTAT/CTAGAA 1 -- ut313Minor allele frequency- C:0.04NA 140
    --
    rs72397211,2
    A,C22059049(+) aaaaaA/Ttattt 1 -- ng51 tfbs30--------
    --
    rs72413031,2
    A,C22060552(+) ACGACC/GTTCCG 1 -- ut510--------
    rs5076131,2
    H22225248(-) CTGTAT/AGTGAT 1 -- ut315Minor allele frequency- A:0.00MN EU EA WA 576
    --
    rs72345781,2
    C22059399(+) cctgtA/Ggttcc 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for TAF4B (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 601689

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for TAF4B gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 6875 HGNC: 11538 AceView: TAF4B Ensembl:ENSG00000141384 euGenes: HUgn6875
    ECgene: TAF4B H-InvDB: TAF4B
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TAF4B:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for TAF4B Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for TAF4B Browse small molecules at Sigma-Aldrich
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