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TAF3 Gene

protein-coding   GIFtS: 50

GC10P007898
TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
(Previous names: TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140 kD )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
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Aliases &
Descriptions
for TAF3

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section
Aliases
MGC25063 2
OTTHUMP00000019072 2
TAF140 1, 2, 3
TAFII140 1, 2, 3, 5
Descriptions
140 kDa TATA box-binding protein-associated factor 3
RNA polymerase II transcription factor TAFII140 2
TAF3 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 140 kD 1, 2
TAF3 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 140kDa 2
TAF3 RNA polymerase II, TATA box binding protein
(TBP)-associated factor, 140kDa (TAF3)(TAF140,TAFII140) 2
TAF3 RNA polymerase II, TATA box binding protein-associated
factor, 140kDa 1
TBP-associated factor 3 3
Transcription initiation factor TFIID 140 kDa subunit 3
External Ids
HGNC: 173031
Entrez Gene: 838602
UniProtKB: Q5VWG93
Ensembl: ENSG000001656327
Search outside databases for aliases for TAF3 gene

Previous GC identifers: GC10P007805 GC10M007824 GC10P008010 GC10P007864

Summaries
for TAF3

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

 EntrezGene summary for TAF3:
The highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises
the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs),
including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic
factors (Gangloff et al., 2001 [PubMed 11438666]).[supplied by OMIM]

UniProtKB/Swiss-Prot: TAF3_HUMAN, Q5VWG9
Function: Transcription factor TFIID is one of the general factors required for accurate and
regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a
central role in mediating promoter responses to various activators and repressors. Required in
complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID
at specific promoters at an early stage in the differentiation process

Gene Wiki entry for TAF3

Genomic Location
for TAF3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

 Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the TAF3 gene  

Entrez Gene cytogenetic band: 10p15.1   Ensembl cytogenetic band:  10p14   HGNC cytogenetic band: 10p15.1

TAF3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P007898:     (about GC identifiers)

Start:
 7,900,507 bp from pter
End:
 8,096,720 bp from pter
Size:
 196,214 bases
Orientation:
 plus strand
RefSeq DNA sequence:
NC_000010.9  NT_077569.2  

Proteins
for TAF3

(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

 UniProtKB/Swiss-Prot: TAF3_HUMAN, Q5VWG9 (See protein sequence)
Recommended Name: Transcription initiation factor TFIID subunit 3  
Size: 929 amino acids; 103582 Da
Subunit: Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number
of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13,
TBP, SAP130 and GCN5L2. Interacts with TBPL2
Subcellular location: Nucleus
Sequence caution: Sequence=AAH45106.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence.
Potential poly-A sequence;
Secondary accessions: Q6GMS5 Q6P6B5 Q86VY6 Q9BQS9 Q9UFI8

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


    • REFSEQ proteins: NP_114129.1  

    ENSEMBL proteins: 
    ENSP00000340271 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (TAF3)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IDA15870280
    About this table

    Antibodies for TAF3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (TAF3), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for TAF3

    Assays for TAF3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    Protein Domains/
    Families
    for TAF3

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    4 InterPro domains/families:
     IPR019786 Zinc_finger_PHD-type_CS
     IPR001965 Znf_PHD
     IPR006565 BTP
     IPR019787 Znf_PHD-finger


       GeneDecks  TAF3 for the domains selected above  
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    Graphical View of Domain Structure for InterPro Entry Q5VWG9

    ProtoNet protein and cluster: Q5VWG9

    1 Blocks protein family: IPB001965 Zn-finger-like

    UniProtKB/Swiss-Prot: TAF3_HUMAN, Q5VWG9
    Similarity: Belongs to the TAF3 family
    Similarity: Contains 1 PHD-type zinc finger

    Gene Function
    for TAF3

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (TAF3)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_031923

                  Applied Biosystems Silencer® siRNAs for TAF3

                  Sigma-Aldrich siRNA for TAF3  
                         Sigma-Aldrich shRNA for TAF3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_031923
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_031923
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_031923 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_031923

    UniProtKB/Swiss-Prot: TAF3_HUMAN, Q5VWG9
    Function: Transcription factor TFIID is one of the general factors required for accurate and
    regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a
    central role in mediating promoter responses to various activators and repressors. Required in
    complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID
    at specific promoters at an early stage in the differentiation process

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0005515 protein binding IPI15870280
    GO:0008270 zinc ion binding IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    Pathways & Interactions
    for TAF3

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Millipore Pathway for TAF3
     Transcription Ligand-Dependent Transcription of Retinoid-Target genes

       GeneDecks  TAF3 for the pathways selected above  
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    2 Sigma-Aldrich "Your Favorite Gene" Pathways for  TAF3  (Your Favorite Gene powered by Ingenuity) 
     Estrogen Receptor Signaling
     Glucocorticoid Receptor Signaling

       GeneDecks  TAF3 for the pathways selected above  
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     Gene Network CentralTM Interacting Genes and Proteins Network for  TAF3 


    5/7 Interacting proteins for TAF3 (ENSP000003402713) via UniProtKB, MINT, and/or STRING (see all 7 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    TAF10ENSP000002994243STRING (score=.957)
    TBPL2ENSP000002472193STRING (score=.949)
    MYOGENSP000002416513STRING (score=.897)
    TBPENSP000002303543STRING (score=.876)
    PDCENSP000003420333STRING (score=.783)
    About this table

    3 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0051457 maintenance of protein location in nucleus IDA15870280
    About this table

    Drugs & Compounds
    for TAF3

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for TAF3

    Transcripts
    for TAF3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section
    Inhib.
    RNA:    		Invitrogen RNAi Products for gene knock-down (TAF3)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_031923

                  Sigma-Aldrich siRNA for TAF3  
                         Sigma-Aldrich shRNA for TAF3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_031923  

    REFSEQ mRNAs for TAF3 gene: 

    NM_031923.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_031923  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_031923
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_031923
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_031923 

    Additional cDNA sequence: AK123254.1 

    5 DOTS entries:

    DT.208158  DT.97836827  DT.202254  DT.91642162  DT.95290801 

    24/74 AceView cDNA sequences (see all 74 ):

    AL117661 AW005174 AA910049 CA397451 AI218666 BC017320 AI002323 AA933921 
    AI364321 AJ292190 AI554630 BQ027547 AA827118 AI554695 BC045106 CD672928 
    AA927947 BC073884 BX094325 BF513064 AI570472 AI363141 BM704312 AI123516 

    highest scoring ESTs for TAF3:

    AA827118 AI123516 AI978684 AW005174 AA777658 AA826691 AA910049 AA918543 AA927947 AA933921 

    Unigene Clusters for TAF3:

    TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
    Hs.527688  [show with all ESTs], Hs.709995  [show with all ESTs]
    Unigene Representative Sequences: NM_031923, AK123254


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000344293  

    Expression
    for TAF3

    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    TAF3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for TAF3

    1 / 2 / 3

    6 probe-sets matching TAF3 gene


    		Affymetrix
    probe-set    		 Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    45078_at2, 3 U95-B 1 1.00 1.00 0.53 1.21 AA827118 0.80 1.00 0.91 1

    		80352_at2, 3 U95-E 1 1.00 1.00 0.70 0.91 AW005174 0.80 1.00 0.91 1
    77419_at2, 3 U95-E 1 1.00 1.00 0.71 1.15 AI978684 0.80 1.00 0.91 1

    		77003_at2, 3 U95-D 1 0.75 1.00 0.62 0.75 AL042512 0.60 1.00 0.82 1

    		235154_at2, 3 U133-B 1 0.91 1.00 -- -- BG250498 0.40 1.00 0.76 1

    		235154_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
    GeneDecks  TAF3 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene clusters: Hs.527688 Hs.709995

    Orthologs
    for TAF3

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section
    Orthologs for TAF3 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity    		 NCBI accessions
    dog
    (Canis familiaris)    		 TAF31   -- TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated more 86.08(n)
    89.56(a)    		 487135  XM_544263.2  XP_544263.2 
    chimpanzee
    (Pan troglodytes)    		 TAF31   -- TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated more 99.53(n)
    99.57(a)    		 450295  XM_001157768.1  XP_001157768.1 
    cow
    (Bos taurus)    		 TAF31   -- TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated more 82.19(n)
    87.46(a)    		 506674  XM_583156.3  XP_583156.3 
    mouse
    (Mus musculus)    		 Taf31, 5 25
    		 TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated more1, 5 83.84(n)1
    86.18(a)1    		 2093611  NM_027748.21  NP_082024.11 
     AA0444915  AA1760545  (see all 23)
    chicken
    (Gallus gallus)    		 TAF31   -- TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated more 75.85(n)
    78.73(a)    		 419107  NM_001030841.1  NP_001026012.1 
    About this table        Species with no ortholog for TAF3

    ENSEMBL Gene Tree for TAF3

    Paralogs
    for TAF3

    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    		  --

    SNPs/Variants
    for TAF3

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/965 NCBI SNPs in TAF3 are shown (see all 965 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 402)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 10 posSequenceRecsAA
    Chg    		TypeMoreRecsAllele
    freq    		PopTotal
    sample    		More
    ------------
    --
    rs107955831,2
    		A,C,F,H8047565(+) agaagG/Ctgaag 1 L/V mis1 trp38Minor allele frequency- C:0.29EU EA WA 836
    rs47476471,2
    		A,C,F,H8046804(+) GAACAG/ATTTCA 1 S/N mis117Minor allele frequency- A:0.42MN EA NA EU WA 1598
    rs12444561,2
    		A,F,H,O7899113(+) gaaatC/Tcaagg 1 -- ng513Minor allele frequency- T:0.33EU EA 298
    rs12444571,2
    		A,F,H,O7899140(+) TGTTAC/TAgaag 1 -- ng514Minor allele frequency- T:0.15EU EA WA 418
    rs20576041,2
    		A,C,F,H7900271(+) GGCGTT/CTTCGT 1 -- ng514Minor allele frequency- C:0.21EU EA WA 420
    --
    rs12442291,2
    		A,C,F,H8047566(+) gaaggT/Cgaagg 1 A/V mis19Minor allele frequency- C:0.50MN EU EA WA 1022
    rs173667121,2
    		C,F,H8046525(+) ACTCAG/CTGAAA 1 T/S mis1 ese311Minor allele frequency- C:0.02NA EU EA WA 982
    rs112553741,2
    		C,F,H7899162(+) ATCAAA/GTAGCT 1 -- ng513Minor allele frequency- G:0.12EU EA 294
    rs12444551,2
    		A,C,H7898974(+) tacacT/Gtgagt 1 -- ng513Minor allele frequency- G:0.00EU EA 290
    rs112553761,2
    		F,H7900444(+) ATTCGG/TCTTAT 1 -- ng514Minor allele frequency- T:0.31EU EA WA 408
    About this table

    HapMap Linkage Disequilibrium images for TAF3 (up to first 250kb)

    Disorders & Mutations
    for TAF3

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    		 OMIM: 606576

    Human Genome Epidemiology Navigator: TAF3 (3 documents)

    Medical News
    for TAF3

    (Possibly Related Articles in Doctor's Guide)
    About This Section

    		   --

    Publications
    for TAF3

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    		10/14 PubMed articles for TAF3 gene (see all 14 ):

    Search for TAF3

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  
      Query String
    		PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    Genome Databases showing TAF3

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section
    Entrez Gene: 83860 HGNC: 17303 AceView: TAF3 Ensembl:ENSG00000165632 euGenes: HUgn83860
    ECgene: TAF3 H-InvDB: TAF3

    Other Databases showing TAF3

    (According to HUGE)
    About This Section

    		   --

    Specialized Databases showing TAF3

    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    		   --

    Licensable Technologies
    for TAF3

    (Available from WIS Yeda, Salk, Tufts)
    About This Section

    		  --

    Services
    for TAF3

    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TAF3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
    		  Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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      Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
      Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for TAF3-related products

     Millipore Custom Antibody & Bulk Services
     Millipore Preclinical / Clinical Development Services
     Millipore Immunoassay Services
     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for TAF3 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for TAF3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search Novus for antibodies for TAF3

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