TAF2 Gene
protein-coding GIFtS : 54
GC08M120812
TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database (Previous symbol: TAF2B )
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Aliases & Descriptions for TAF2
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases CIF150 1 , 2 , 3 TAF2B 2 , 3 TAFII-150 3 TAFII150 1 , 2 , 3
Descriptions 150 kDa cofactor of initiator function 3 RNA polymerase II TBP-associated factor subunit B 3 TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150 kD 2 TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa 2 TAF2 RNA polymerase II, TATA box binding protein-associated factor, 150kDa 1 TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD 1 , 2 TBP-associated factor 150 kDa 3 TBP-associated factor 2 2 Transcription initiation factor TFIID 150 kDa subunit 3 cofactor of initiator function, 150kD subunit 2
Search outside databases for aliases for TAF2 genePrevious GC identifers: GC08M119730 GC08M120749 GC08M120411 GC08M120699
Summaries for TAF2 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for TAF2 : Initiation of transcription by RNA polymerase II requires the activities of more than 70polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID),which binds to the core promoter to position the polymerase properly, serves as the scaffold forassembly of the remainder of the transcription complex, and acts as a channel for regulatorysignals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarilyconserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basaltranscription, serve as coactivators, function in promoter recognition or modify generaltranscription factors (GTFs) to facilitate complex assembly and transcription initiation. Thisgene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex.It contributes to interactions at and downstream of the transcription initiation site,interactions that help determine transcription complex response to activators. [provided byRefSeq] UniProtKB/Swiss-Prot: TAF2_HUMAN, Q6P1X5 Function : Transcription factor TFIID is one of the general factors required for accurate andregulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays acentral role in mediating promoter responses to various activators and repressors. It requirescore promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIIDbinding to core promoter
Gene Wiki entry for TAF2
Genomic Location for TAF2
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the TAF2 gene Entrez Gene cytogenetic band: 8q24.12 Ensembl cytogenetic band: 8q24.12 HGNC cytogenetic band: 8q24 TAF2 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 8 GeneLoc Exon Structure
GeneLoc location for GC08M120812:
(about GC identifiers )
Start:
120,812,195 bp from pter
End:
120,914,255 bp from pter
Size:
102,061 bases
Orientation:
minus strand
RefSeq DNA sequence: NC_000008.9 NT_008046.15 Proteins for TAF2
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: TAF2_HUMAN, Q6P1X5 (See
protein sequence )Recommended Name: Transcription initiation factor TFIID subunit 2 Size : 1199 amino acids; 136971 Da
Subunit : Component of transcription factor TFIID which is composed of TBP and a number ofTBP-associated factors. Interacts with TAF2C1. Component of the TFTC-HAT complex
Subcellular location : Nucleus
Miscellaneous : PubMed:9418870 was unable to show an association between TAF2 and TFIID
Sequence caution : Sequence=AAH64830.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence.Potential poly-A sequence;
Secondary accessions : B2RE82 O43487 O43604 O60668 Q86WW7 Q8IWK4
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins: NP_003175.1 ENSEMBL proteins: ENSP00000367406 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 2 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for TAF2: Assays for TAF2:
Protein
Domains/ Families for TAF2(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q6P1X5 ProtoNet protein and cluster: Q6P1X5
1 Blocks protein family : IPB001930 Membrane alanyl dipeptidase (M1) family signature UniProtKB/Swiss-Prot: TAF2_HUMAN, Q6P1X5 Similarity : Belongs to the TAF2 family
Gene Function for TAF2
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
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Sigma-Aldrich , RNAi from
Sigma-Aldrich ,
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SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003184 Applied Biosystems Silencer ® siRNAs for TAF2 Sigma-Aldrich siRNA for TAF2 Sigma-Aldrich shRNA for TAF2 Explore Sigma-Aldrich super-pooled esiRNAs               OriGene GFP tagged cDNA clone in CMV expression vector: NM_003184                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_003184                                  untagged cDNA clone in CMV expression vector: NM_003184  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_003184 UniProtKB/Swiss-Prot: TAF2_HUMAN, Q6P1X5 Function : Transcription factor TFIID is one of the general factors required for accurate andregulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays acentral role in mediating promoter responses to various activators and repressors. It requirescore promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIIDbinding to core promoter
3 Gene Ontology (GO) molecular function terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0003702 RNA polymerase II transcription factor activity
TAS 9418870 GO:0008237 metallopeptidase activity
IEA -- GO:0008270 zinc ion binding
IEA --
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Pathways & Interactions for TAF2
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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4 Sigma-Aldrich "Your Favorite Gene" Pathways for TAF2 (Your Favorite Gene powered by Ingenuity) Gene Network CentralTM Interacting Genes and Proteins Network for TAF2 3 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0006350 transcription
IEA -- GO:0006355 regulation of transcription, DNA-dependent
IEA -- GO:0006367 transcription initiation from RNA polymerase II promoter
TAS 9418870
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Drugs & Compounds for TAF2 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Browse Tocris compounds for TAF2
Transcripts for TAF2(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
Sigma-Aldrich ,
shRNA from
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OriGene ,
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003184 Sigma-Aldrich siRNA for TAF2 Sigma-Aldrich shRNA for TAF2 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_003184
REFSEQ mRNAs for TAF2 gene: NM_003184.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_003184
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_003184                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_003184                                  untagged cDNA clone in CMV expression vector: NM_003184  
Additional cDNA sequence: AF026445.1 AF040701.1 AF057694.1 AK001618.1 AK001909.1 AK316592.1 AL137601.1 BC008473.1 BC018010.1 BC035673.2 BC037262.1 BC047732.1 BC064830.1
11 DOTS entries : DT.210351 DT.95172572 DT.100862526 DT.101976333 DT.120632559 DT.92039731 DT.100643486 DT.102828677 DT.91820931 DT.95172559 DT.92435446
24/149 AceView cDNA sequences (see all 149
):BQ427717 AI990807 AI420493 AL137601 AU132255 AI373838 AI653879 BQ959501 AI393239 AA594294 AA768610 AU098502 BE645277 NM_003184 AI358531 CA416456 AL040173 BX091764 AK001909 BM974263 BF001811 AW963035 BX490736 AF026445
highest scoring ESTs for TAF2 :AF026445 AF040701 AF057694 AI694287 AL040173 AU098502 BC035673 BC047732 BC064830 BM558517
Unigene Cluster for TAF2: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa Hs.122752 [show with all ESTs ] Unigene Representative Sequence: NM_003184 GeneLoc Exon Structure 5/8 Alternative Splicing Database (ASD) splice patterns (SP) for TAF2 (see all 8
) ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19a · 19b · 19c ^ SP1 :                                               -       SP2 :                                                     SP3 :                                                     SP4 :                                                     SP5 :           -                                          
ExUns: 20a · 20b ^ 21a · 21b · 21c ^ 22 ^ 23a · 23b ^ 24a · 24b ^ 25a · 25b ^ 26 ^ 27 ^ 28a · 28b SP1 : -   -       -             -   -           SP2 :         -             -   -           SP3 :         -                         SP4 :                                 SP5 :                                
About this scheme ECgene alternative splicing isoforms for TAF2 1 Ensembl transcript including schematic representation : ENST00000378164
Expression for TAF2
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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TAF2 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for TAF2 1 / 2 / 3
6 probe-sets matching TAF2 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: TGCTTTAATASOURCE GeneReport for Unigene cluster: Hs.122752 UniProtKB/Swiss-Prot: TAF2_HUMAN, Q6P1X5 Tissue specificity : Expressed in all tissues tested
Orthologs for TAF2
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for TAF2 gene from 5/14 species (see all 14
)
About this table Species with no ortholog for TAF2 ENSEMBL Gene Tree for TAF2 Paralogs for TAF2 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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SNPs/Variants for TAF2 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for TAF2 (up to first 250kb)
Disorders & Mutations for TAF2
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 604912
Medical News for TAF2 (Possibly Related Articles in
Doctor's Guide )
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Publications for TAF2 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/33 PubMed articles for TAF2 gene (see all 33
): Novel cofactors and TFIIA mediate functional core promoter selectivity by the human TAFII150-containing TFIID complex. (PubMed id 9774672) 2, 3, 4 Martinez E.... Roeder R.G. (1998) CIF150, a human cofactor for transcription factor IID-dependent initiator function. (PubMed id 9418870) 2, 3, 4 Kaufmann J....Muller R. (1998) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983) 3, 4 Olsen J.V....Mann M. (2006) Global phosphoproteome of HT-29 human colon adenocarcinoma cells. (PubMed id 16083285) 3, 4 Kim J.-E.... White F.M. (2005) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 3, 4 Ota T....Sugano S. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis. (PubMed id 12601814) 3, 4 Cavusoglu N....Van Dorsselaer A. (2003) Automated phosphoproteome analysis for cultured cancer cells by two- dimensional nanoLC-MS using a calcined titania/C18 biphasic column. (PubMed id 18187866) 4 Imami K.... Ishihama Y. (2008) A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648) 4 Dephoure N.... Gygi S.P. (2008) DNA sequence and analysis of human chromosome 8. (PubMed id 16421571) 4 Nusbaum C....Lander E.S. (2006)
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Aliases
TAF2 (Gene Symbol) CIF150 TAF2B TAFII-150 TAFII150 150 kDa cofactor of initiator function RNA polymerase II TBP-associated factor subunit B TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150 kD TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa TAF2 RNA polymerase II, TATA box binding protein-associated factor, 150kDa TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD TBP-associated factor 150 kDa TBP-associated factor 2 Transcription initiation factor TFIID 150 kDa subunit cofactor of initiator function, 150kD subunit
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Genome Databases showing TAF2
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing TAF2
(According to HUGE )
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Specialized Databases showing TAF2 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Incremental update: 13 Oct 2009