TAF1B Gene
protein-coding GIFtS : 51
GC02P009934
TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa (Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kD )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
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Aliases & Descriptions for TAF1B
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases MGC:9349 2 RAF1B 1 , 2 RAFI63 1 , 2 SL1 1 , 2 TAFI63 1 , 2 , 3
Descriptions SL1, 63kD subunit 2 TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kD 1 TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa 2 TATA box binding protein-associated factor, RNA polymerase I, B, 63kDa 1 TATA box-binding protein-associated factor 1B 3 TBP-associated factor 1B 2 , 3 TBP-associated factor RNA polymerase I 63 kDa 3 Transcription factor SL1 3
Search outside databases for aliases for TAF1B genePrevious GC identifers: GC02P009767 GC02P010034 GC02P010006 GC02P010007
Summaries for TAF1B (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for TAF1B : Initiation of transcription by RNA polymerase I requires the formation of a complex composed of theTATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I.This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position thepolymerase properly and acts as a channel for regulatory signals. This gene encodes one of theSL1-specific TAFs. [provided by RefSeq] UniProtKB/Swiss-Prot: TAF1B_HUMAN, Q53T94 Function : Component of the transcription factor SL1/TIFIB complex involved in the assembly of thePIC (preinitiation complex)
Gene Wiki entry for TAF1B
Genomic Location for TAF1B
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
Genomic Views according to
UCSC and
Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the TAF1B gene Entrez Gene cytogenetic band: 2p25 Ensembl cytogenetic band: 2p25.1 HGNC cytogenetic band: 2p25 TAF1B Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 2 GeneLoc Exon Structure
GeneLoc location for GC02P009934:
(about GC identifiers )
Start:
9,890,887 bp from pter
End:
9,991,996 bp from pter
Size:
101,110 bases
Orientation:
plus strand
RefSeq DNA sequence: NC_000002.10 NT_005334.15 Proteins for TAF1B
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: TAF1B_HUMAN, Q53T94 (See
protein sequence )Recommended Name: TATA box-binding protein-associated factor RNA polymerase I subunit B Size : 588 amino acids; 68832 Da
Subunit : Component of the transcription factor SL1/TIFIB complex which is composed of TBP and atleast TAF1A, TAF1B and TAF1C. In the complex, interacts directly with TBP, TAF1A and TAF1C.Interaction of the SL1/TIFIB subunits with TBP excludes interaction with the transcription factorIID (TFIID) subunits. This mutually exclusive binding directs the formation of promoter and RNApolymerase-selective TBP-TAF complexes
Subcellular location : Nucleus
Secondary accessions : Q15574 Q8WVC3Alternative splicing : 2 isoforms : Q53T94-1 Q53T94-2
REFSEQ proteins: NP_005671.2 ENSEMBL proteins: ENSP00000384598 ENSP00000385959 ENSP00000263663 ENSP00000379540 ENSP00000379542 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 2 Gene Ontology (GO) cellular component terms (links to tree view) :
About this table Antibodies for TAF1B: Assays for TAF1B:
Protein
Domains/ Families for TAF1B(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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ProtoNet protein and cluster: Q53T94
UniProtKB/Swiss-Prot: TAF1B_HUMAN, Q53T94 Similarity : Belongs to the TAF1 family
Gene Function for TAF1B
(According to MGI Jun 06 2009, UniProtKB ,
IUBMB ,and/or Genatlas ,
shRNA from
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Sigma-Aldrich ,
RNAi Products ,
Clones , and
Q-PCR Products
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Millipore ,
OriGene , and/or
Abnova ,
siRNAs from
Applied Biosystems ,
SYBR primers from OriGene ,
Cell-based Assays from Millipore ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005680 Applied Biosystems Silencer ® siRNAs for TAF1B Sigma-Aldrich siRNA for TAF1B Sigma-Aldrich shRNA for TAF1B Explore Sigma-Aldrich super-pooled esiRNAs               OriGene GFP tagged cDNA clone in CMV expression vector: NM_005680                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_005680                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_005680  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_005680 UniProtKB/Swiss-Prot: TAF1B_HUMAN, Q53T94 Function : Component of the transcription factor SL1/TIFIB complex involved in the assembly of thePIC (preinitiation complex)
Genatlas biochemistry entry for TAF1B :template activating factor I,specifically interactin with mitotic cyclin B 1 Gene Ontology (GO) molecular function term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0003700 transcription factor activity
NAS 7801123
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Pathways & Interactions for TAF1B
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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Gene Network CentralTM Interacting Genes and Proteins Network for TAF1B 5/27 Interacting proteins for TAF1B (Q53T94 2 ENSP00000263663 3 ) via UniProtKB, MINT, and/or STRING (see all 27
)About this table 1 Gene Ontology (GO) biological process term (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0006355 regulation of transcription, DNA-dependent
IEA --
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Drugs & Compounds for TAF1B (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
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Novoseek and Drugs according to
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PharmGKB )
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Transcripts for TAF1B(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
Abnova ,
siRNAs from Applied Biosystems ,
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shRNA from
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              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005680 Sigma-Aldrich siRNA for TAF1B Sigma-Aldrich shRNA for TAF1B Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_005680
REFSEQ mRNAs for TAF1B gene: NM_005680.2
Applied Biosystems TaqMan ® Gene Expression Assays: NM_005680
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_005680                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_005680                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_005680  
Additional cDNA sequence: AK026521.1 AK124192.1 AK295402.1 AK308070.1 AK309751.1 AK315098.1 BC018137.2 CR606132.1 CR616493.1 CR622846.1 L39061.1
17 DOTS entries : DT.210623 DT.101975420 DT.91752588 DT.91742818 DT.100777167 DT.300603 DT.100667187 DT.120968403 DT.120968441 DT.91752591 DT.100777166 DT.40125689 DT.91752592 DT.95107044 DT.99947775 DT.100743103 DT.95258536
24/158 AceView cDNA sequences (see all 158
):CR622846 AA620887 AA811940 CR606132 CR616493 CA423871 AA746267 AI436217 AW954208 F09444 BX117196 BX371656 AL528401 BC018137 AA534600 AA702292 AI188257 BI918201 BX425268 AI188329 BG527360 BQ011382 BX472202 BP353583
highest scoring ESTs for TAF1B :AI968709 AA004579 AA702292 AA746267 AK026521 AL528369 AL571515 AW193501 AW873654 BC018137
Unigene Cluster for TAF1B: TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa Hs.584833 [show with all ESTs ] Unigene Representative Sequence: CR622846 GeneLoc Exon Structure 5 Ensembl transcripts including schematic representations : ENST00000404869
ENST00000402170
ENST00000263663
ENST00000396240
ENST00000396242
Expression for TAF1B
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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TAF1B expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for TAF1B 1 / 2 / 3
7 probe-sets matching TAF1B gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: AAGAAGTGAGSOURCE GeneReport for Unigene cluster: Hs.584833 Expression variation in blood from EXPOLDB for TAF1B
Orthologs for TAF1B
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for TAF1B gene from 5/7 species (see all 7
)
About this table Species with no ortholog for TAF1B ENSEMBL Gene Tree for TAF1B Paralogs for TAF1B (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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SNPs/Variants for TAF1B (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for TAF1B (up to first 250kb)
Disorders & Mutations for TAF1B
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
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Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 604904
Medical News for TAF1B (Possibly Related Articles in
Doctor's Guide )
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Publications for TAF1B (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/17 PubMed articles for TAF1B gene (see all 17
): Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits. (PubMed id 7801123) 2, 3, 4 Comai L....Tjian R. (1994) Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PubMed id 15815621) 3, 4 Hillier L.W....Wilson R.K. (2005) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1. (PubMed id 10894955) 1, 3 Di Pietro C....Purrello M. (2000) Identification of MARCKS, FLJ11383 and TAF1B as putative novel target genes in colorectal carcinomas with microsatellite instability. (PubMed id 12140758) 1, 3 Kim N.G....Kim H. (2002) Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. (PubMed id 17456004) 3 You K.T....Kim H. (2007) Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560) 3 Kimura K.... Sugano S. (2006) c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. (PubMed id 15723054) 3 Grandori C.... White R.J. (2005) Rrn3 becomes inactivated in the process of ribosomal DNA transcription. (PubMed id 12646563) 3 Hirschler-Laszkiewicz I....Rothblum L.I. (2003) Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter. (PubMed id 12393749) 3 Yuan X.... Grummt I. (2002)
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TAF1B (Gene Symbol) MGC:9349 RAF1B RAFI63 SL1 TAFI63 SL1, 63kD subunit TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kD TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa TATA box binding protein-associated factor, RNA polymerase I, B, 63kDa TATA box-binding protein-associated factor 1B TBP-associated factor 1B TBP-associated factor RNA polymerase I 63 kDa Transcription factor SL1
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Genome Databases showing TAF1B
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing TAF1B
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Specialized Databases showing TAF1B (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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