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TAF1A Gene

protein-coding   GIFtS: 56

GC01M220797
TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
(Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kD )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
MGC:17061 2
OTTHUMP00000035653 2
OTTHUMP00000035654 2
RAFI48 2, 5
SL1 1, 2, 5
TAFI48 1, 2, 3
Descriptions
SL1, 48kD subunit 2
TATA box binding protein (TBP)-associated factor, RNA
polymerase I, A, 48kD 1
TATA box binding protein (TBP)-associated factor, RNA
polymerase I, A, 48kDa 2
TATA box binding protein-associated factor, RNA polymerase
I, A, 48kDa 1
TATA box-binding protein-associated factor 1A 3
TBP-associated factor 1A 2, 3
TBP-associated factor RNA polymerase I 48 kDa 3
Transcription factor SL1 3
External Ids
HGNC: 115321
Entrez Gene: 90152
UniProtKB: Q155733
Ensembl: ENSG000001434987
Search outside databases for aliases for TAF1A gene

Previous GC identifers: GC01M221325 GC01M218646 GC01M219263 GC01M219787 GC01M219119

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for TAF1A:
Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the
TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I.
This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the
polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest
SL1-specific TAF. Two transcripts encoding different isoforms have been identified. [provided by
RefSeq]

UniProtKB/Swiss-Prot: TAF1A_HUMAN, Q15573
Function: Component of the transcription factor SL1/TIFIB complex involved in the assembly of the
PIC (preinitiation complex)

Gene Wiki entry for TAF1A

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q42   Ensembl cytogenetic band:  1q41   HGNC cytogenetic band: 1q42

TAF1A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M220797:     (about GC identifiers)

Start:
220,786,452 bp from pter
End:
220,829,878 bp from pter
Size:
43,427 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000001.9  NT_021877.18  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: TAF1A_HUMAN, Q15573 (See protein sequence)
Recommended Name: TATA box-binding protein-associated factor RNA polymerase I subunit A  
Size: 450 amino acids; 52676 Da
Subunit: Component of the transcription factor SL1/TIFIB complex which is composed of TBP and at
least TAF1A, TAF1B and TAF1C. In the complex, interacts directly with TBP, TAF1B and TAF1C.
Interaction of the SL1/TIFIB subunits with TBP excludes interaction with the transcription factor
IID (TFIID) subunits. This mutually exclusive binding directs the formation of promoter and RNA
polymerase-selective TBP-TAF complexes
Subcellular location: Nucleus
Secondary accessions: Q9NWA1
Alternative splicing: 2 isoforms:  Q15573-1   Q15573-2   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_005672.1  NP_647603.1  


    ENSEMBL proteins: 
    ENSP00000375755 ENSP00000327072 ENSP00000339976 ENSP00000375754 ENSP00000355856 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Sigma-Aldrich AQUA Peptides for TAF1A  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (TAF1 48)
    Human Recombinant Proteins from Abnova (TAF1A)
                   OriGene Purified Recombinant Human Proteins (see all 2): TAF1A  

    3 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000120 RNA polymerase I transcription factor complex IEA--
    GO:0005634 nucleus IEA--
    GO:0005654 nucleoplasm EXP9582279 11250901 12393749 12646563
    About this table

    Antibodies for TAF1A: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for TAF1A
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (TAF1 48), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (TAF1A)
    Novus Biologicals Antibodies for TAF1A

    Assays for TAF1A: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR016629 RNA_pol_I_TAF1A/TAFI48


       GeneDecks  TAF1A for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q15573

    ProtoNet protein and cluster: Q15573

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TAF1A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TAF1A)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005681

                  Applied Biosystems Silencer® siRNAs for TAF1A

                  Sigma-Aldrich siRNA for TAF1A  
                         Sigma-Aldrich shRNA for TAF1A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for TAF1A
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_005681
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_005681
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_005681 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005681

    UniProtKB/Swiss-Prot: TAF1A_HUMAN, Q15573
    Function: Component of the transcription factor SL1/TIFIB complex involved in the assembly of the
    PIC (preinitiation complex)

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0003701 RNA polymerase I transcription factor activity TAS7801123
    GO:0005515 protein binding IEA--
    GO:0016251 general RNA polymerase II transcription factor activity TAS7801123
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  TAF1A 


    5/28 Interacting proteins for TAF1A (Q155732 ENSP000003270723) via UniProtKB, MINT, and/or STRING (see all 28 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SETQ011052, ENSP000003617773MINT-4544075 MINT-4543938 STRING (score=.894)
    TAF1BQ53T942, ENSP000002636633MINT-4543890 STRING (score=.999)
    TBPP202262, ENSP000002303543MINT-4544108 STRING (score=.998)
    UBTFENSP000003026403STRING (score=.987)
    RRN3ENSP000001987673STRING (score=.96)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0006360 transcription from RNA polymerase I promoter TAS7801123
    GO:0006366 transcription from RNA polymerase II promoter TAS7801123
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for TAF1A
    1 Novoseek chemical compound relationship for TAF1A gene
    Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    s-napc 93.33 1 8339931 (1)
    About this table


    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (TAF1A)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (TAF1A)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_005681

                  Sigma-Aldrich siRNA for TAF1A  
                         Sigma-Aldrich shRNA for TAF1A  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005681  NM_139352  

    REFSEQ mRNAs for TAF1A gene (2 alternative transcripts): 

    NM_005681.2   NM_139352.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005681  NM_139352  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_005681
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_005681
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_005681 

    Additional cDNA sequence: 

    AK290970.1 AK299536.1 AK307882.1 AK315740.1 BC013808.1 CR612936.1 L39060.1 

    7 DOTS entries:

    DT.209757  DT.100021120  DT.40118127  DT.100752251  DT.100739188  DT.91738658  DT.91738665 

    24/36 AceView cDNA sequences (see all 36 ):

    BQ051078 BM841948 L39060 N66196 AU118970 BU179101 BM845364 BU192019 
    BX118609 BU839228 BX373108 N39785 AA743681 BQ574624 BC013808 AL527402 
    BG505180 BI089656 BX505527 AI292312 BE895004 AU145413 BI831878 AA305702 

    highest scoring ESTs for TAF1A:

    L39060 AL527402 AL711246 BC013808 BE895004 BI089656 BI755870 BU179101 BX505527 CD639754 

    Unigene Cluster for TAF1A:

    TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa
    Hs.153088  [show with all ESTs]
    Unigene Representative Sequence: AK290970


    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for TAF1A (see all 7 )

    ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13
    SP1:                                            -                                               -                                       
    SP2:                                      -     -                                               -                             -         
    SP3:                                            -     -                                                                                 
    SP4:                                            -     -     -                                                                           
    SP5:                                                                                                                                    

    About this scheme

    ECgene alternative splicing isoforms for TAF1A

    5 Ensembl transcripts including schematic representations:
    ENST00000391883  ENST00000352967  ENST00000350027  ENST00000391882  ENST00000366890  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    TAF1A expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for TAF1A

    1 / 2 / 3

    3 probe-sets matching TAF1A gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    35006_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 L39060 1.00 1.00 1.00 1

    206613_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_005681 0.60 1.00 0.82 1

    206613_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GTGAAAATAC

    SOURCE GeneReport for Unigene cluster: Hs.153088

    Expression variation in blood from EXPOLDB for TAF1A

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for TAF1A gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    TAF1A1   -- TATA box binding protein (TBP)-associated factor, RNA more 87.33(n)
    82.67(a)
    608850  XM_845987.1  XP_851080.1 
    chimpanzee
    (Pan troglodytes)
    TAF1A1   -- TATA box binding protein (TBP)-associated factor, RNA more 99.56(n)
    99.33(a)
    747636  XM_001173109.1  XP_001173109.1 
    cow
    (Bos taurus)
    TAF1A1   -- TATA box binding protein (TBP)-associated factor, RNA more 85.78(n)
    79.56(a)
    505051  NM_001045908.1  NP_001039373.1 
    rat
    (Rattus norvegicus)
    Taf1a1   -- TATA box binding protein (Tbp)-associated factor, RNA more 80.8(n)
    77.9(a)
    360893  NM_001037204.1  NP_001032281.1 
    mouse
    (Mus musculus)
    Taf1a1, 5 15
    TATA box binding protein (Tbp)-associated factor, RNA more1, 5 81.03(n)1
    79.91(a)1
    213391  NM_021466.21  NP_067441.11 
     AA6239495  AK1328115  (see all 10)
    About this table        Species with no ortholog for TAF1A

    ENSEMBL Gene Tree for TAF1A
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/151 NCBI SNPs in TAF1A are shown (see all 151 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 89)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 1 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs30086531,2
    C,F,H,O220831414(+) AAACAC/GCTCTA 2 -- ng317Minor allele frequency- G:0.11EU EA WA NA 560
    rs30086501,2
    C,F,H220829838(+) CGGCCG/CGGCTC 2 -- ut51 ese38Minor allele frequency- C:0.41EA NA EU WA 636
    rs29360401,2
    C,F,H220830284(-) AAGGCA/TAAGGA 2 -- ng318Minor allele frequency- T:0.10EU EA WA 838
    rs171632711,2
    C,F220819835(+) CAAATA/CATCTG 2 I/M mis1 ut517Minor allele frequency- C:0.02NA EU EA WA 560
    rs23785931,2
    A220831474(+) CAATTC/TTGACA 2 -- ng310--------
    --
    rs40760811,2
    C220797853(+) tttttG/Tttttt 2 -- ng510--------
    rs30086511,2
    A220829858(+) CCTGCG/TGCAGC 2 -- ut51 ese30--------
    --
    rs11348981,2
    C,F,O220801426(-) CCAGGC/GTTTCA 2 G/G syn13Minor allele frequency- G:0.31NA 140
    rs29360411,2
    C,F,H,O220829396(-) TTTGAT/ATTTAG 2 -- int111Minor allele frequency- A:0.45EU EA WA NA 980
    rs29360321,2
    C,F,O220818414(-) ACCCAA/GGGAAC 2 -- int13Minor allele frequency- G:0.24NA 140
    About this table

    HapMap Linkage Disequilibrium images for TAF1A (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 604903

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/23 PubMed articles for TAF1A gene (see all 23 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 9015 HGNC: 11532 AceView: TAF1A.1 Ensembl:ENSG00000143498 euGenes: HUgn9015
    ECgene: TAF1A H-InvDB: TAF1A
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for TAF1A:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Explore super-pooled esiRNAs Proteins for TAF1A
     Lentivirus-delivered shRNAs for TAF1A Browse small molecules at Sigma-Aldrich
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     Purified proteins from HEK293T cells
     Western blot validated antibodies
     shRNA in GFP-retroviral vector
     Search Tocris compounds for TAF1A




     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
     Antibodies for TAF1A

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