TAF12 Gene
protein-coding GIFtS : 62
GC01M028801
TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa (Previous names: TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD )Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database (Previous symbol: TAF2J )
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Aliases & Descriptions for TAF12
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc
, and/or 7 Ensembl ,
8 miRBase )About This Section
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Aliases OTTHUMP00000003785 2 OTTHUMP00000003786 2 TAF15 3 TAF2J 2 , 3 TAFII-20/TAFII-15 3 TAFII20 1 , 2 , 3 TAFII20/TAFII15 3
Descriptions TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20 kD 2 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa 2 TAF12 RNA polymerase II, TATA box binding protein-associated factor, 20kDa 1 TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD 1 , 2 Transcription initiation factor TFIID 20/15 kDa subunits 2 , 3
Search outside databases for aliases for TAF12 genePrevious GC identifers: GC01P028822 GC01M027888 GC01M028434 GC01M028617 GC01M028750
Summaries for TAF12 (According to Entrez Gene ,
Wikipedia's
Gene Wiki ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
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EntrezGene summary for TAF12 : Control of transcription by RNA polymerase II involves the basal transcription machinery which is acollection of proteins. These proteins with RNA polymerase II, assemble into complexes which aremodulated by transactivator proteins that bind to cis-regulatory elements located adjacent to thetranscription start site. Some modulators interact directly with the basal complex, whereas othersmay act as bridging proteins linking transactivators to the basal transcription factors. Some ofthese associated factors are weakly attached while others are tightly associated with TBP in theTFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate thefunction of distinct transcriptional activators for a variety of gene promoters and RNApolymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variantsencoding the same protein have been found for this gene. [provided by RefSeq] UniProtKB/Swiss-Prot: TAF12_HUMAN, Q16514 Function : TAFs are components of the transcription factor IID (TFIID) complex, PCAF histoneacetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential formediating regulation of RNA polymerase transcription
Gene Wiki entry for TAF12
Genomic Location for TAF12
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 36) ,
and/or miRBase ,
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Ensembl ,
Transcription factor binding sites according to
SABiosciences )About This Section
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Genomic View : UCSC Golden Path with GeneCards custom track Transcription factor binding sites upstream to the TAF12 gene Entrez Gene cytogenetic band: 1p35.3 Ensembl cytogenetic band: 1p35.3 HGNC cytogenetic band: 1p35 TAF12 Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc gene densities for chromosome 1 GeneLoc Exon Structure
GeneLoc location for GC01M028801:
(about GC identifiers )
Start:
28,802,200 bp from pter
End:
28,842,172 bp from pter
Size:
39,973 bases
Orientation:
minus strand
RefSeq DNA sequence: NC_000001.9 NT_004610.18 Proteins for TAF12
(According to
1 UniProtKB ,
and/or Ensembl ,
Phosphorylation sites according to 2 Phosphosite ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Enzo Life Sciences ,
Abnova ,
OriGene and/or,
Abcam ,
Biochemical Assays by
Invitrogen ,
Millipore ,
R&D Systems ,
Cell Signaling Technology , and/or
Enzo Life Sciences ,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene ,
Antibodies by Invitrogen ,
Millipore ,
Sigma-Aldrich ,
R&D Systems ,
Cell Signaling Technology ,
Abcam ,
Abnova , and/or
Novus Biologicals )
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UniProtKB/Swiss-Prot: TAF12_HUMAN, Q16514 (See
protein sequence )Recommended Name: Transcription initiation factor TFIID subunit 12 Size : 161 amino acids; 17924 Da
Subunit : TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associatedfactors (TAFs). TBP is not part of TFTC. Interacts directly with TBP; additional interactionsbetween TAFII20 and TAFII28 or TAFII30 were detected. Component of the PCAF complex, at leastcomposed of TADA2L/ADA2, TADA3L/ADA3, TAF5L/PAF65-beta, SUPT3H, TAF6L, TAF9, TAF10, TAF12 andTRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H,GCN5L2, TAF5L, TAF6L, STAF65-gamma/KIAA0764, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9
Subcellular location : Nucleus
PDB structures from and Proteopedia : 1H3O (3D)
 
Secondary accessions : Q15775 Q5T077Alternative initiation : 2 isoforms : Q16514-1 Q16514-2
Post-translational modifications:
View phosphorylation sites using PhosphoSite 2
REFSEQ proteins (2 alternative transcripts):
NP_001128690.1 NP_005635.1 ENSEMBL proteins: ENSP00000263974 ENSP00000362930 Human Recombinant Proteins Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 5/6 Gene Ontology (GO) cellular component terms (links to tree view) (see all 6
):
About this table Antibodies for TAF12: Assays for TAF12:
Protein
Domains/ Families for TAF12(According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
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Graphical View of Domain Structure for InterPro Entry Q16514 ProtoNet protein and cluster: Q16514
1 Blocks protein family : IPB003228 Transcription initiation factor TFIID UniProtKB/Swiss-Prot: TAF12_HUMAN, Q16514 Similarity : Belongs to the TAF12 familySimilarity : Contains 1 histone-fold domain
Gene Function for TAF12
(According to MGI Jun 06 2009, UniProtKB ,
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SYBR primers from OriGene ,
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Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_005644 Applied Biosystems Silencer ® siRNAs for TAF12 Sigma-Aldrich siRNA for TAF12 Sigma-Aldrich shRNA for TAF12 Explore Sigma-Aldrich super-pooled esiRNAs                OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001135218                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001135218                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001135218  Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_001135218 UniProtKB/Swiss-Prot: TAF12_HUMAN, Q16514 Function : TAFs are components of the transcription factor IID (TFIID) complex, PCAF histoneacetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential formediating regulation of RNA polymerase transcription
4 Gene Ontology (GO) molecular function terms (links to tree view) :
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Pathways & Interactions for TAF12
(Pathways according to Invitrogen
(maps by GeneGo ),
Millipore ,
Cell Signaling Technology ,
Sigma-Aldrich ,
KEGG
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Proteins Network according to
SABiosciences ,
Interactions according to 1 UniProtKB ,
2 MINT , and/or
3 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene .)
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2 Sigma-Aldrich "Your Favorite Gene" Pathways for TAF12 (Your Favorite Gene powered by Ingenuity) Gene Network CentralTM Interacting Genes and Proteins Network for TAF12 5/88 Interacting proteins for TAF12 (Q16514 2 ENSP00000263974 3 ) via UniProtKB, MINT, and/or STRING (see all 88
)About this table 4 Gene Ontology (GO) biological process terms (links to tree view) :
GO ID Qualified GO term Evidence PubMed IDs GO:0006350 transcription
IEA -- GO:0006355 regulation of transcription, DNA-dependent
IEA -- GO:0006367 transcription initiation from RNA polymerase II promoter
EXP 8946909 GO:0006368 RNA elongation from RNA polymerase II promoter
EXP 9405375
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Drugs & Compounds for TAF12 (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
Sigma-Aldrich , Tocris Bioscience , and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB )
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Transcripts for TAF12(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
non coding RNAs according to
RNAdb ,
ESTs according to GeneTide ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from Invitrogen ,
Millipore , and/or
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               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2 ): NM_005644 Sigma-Aldrich siRNA for TAF12 Sigma-Aldrich shRNA for TAF12 Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer ® siRNAs: NM_001135218 NM_005644
REFSEQ mRNAs for TAF12 gene (2 alternative transcripts): NM_001135218.1 NM_005644.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_001135218 NM_005644
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2 ): NM_001135218                                  Myc/DDK tagged cDNA clones in CMV expression vector (see all 2 ): NM_001135218                                  untagged cDNA clones in CMV expression vector (see all 2 ): NM_001135218  
Additional cDNA sequence: AK310097.1 BC011986.1 BT007031.1 CR590266.1 D50544.1 U57693.1 X84002.1
5 DOTS entries : DT.315898 DT.99988669 DT.91747947 DT.91747950 DT.91747956
24/205 AceView cDNA sequences (see all 205
):CA389592 BF590981 BQ019838 CA430306 CA422908 AI284938 AI084878 AA721251 CA309894 BF797751 CF128243 BE551284 BX095156 AI298813 H85093 CD672328 BI088190 AW166605 BU192210 BM141667 CR590266 AI281331 AW513240 AA280901
highest scoring ESTs for TAF12 :U57693 X84002 AA045587 AA316756 AA374220 AW954402 BC011986 BE439962 BE564825 BE564887
Unigene Cluster for TAF12: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa Hs.530251 [show with all ESTs ] Unigene Representative Sequence: BF217719 GeneLoc Exon Structure 4 Alternative Splicing Database (ASD) splice patterns (SP) for TAF12 ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b SP1 :       -   -   -                   SP2 :       -     -                   SP3 :         -   -                   SP4 :                            
About this scheme ECgene alternative splicing isoforms for TAF12 2 Ensembl transcripts including schematic representations : ENST00000263974
ENST00000373824
Expression for TAF12
(Experimental results according to
1 GeneNote
and GNF BioGPS ,
probe sets-to-genes annotations according to
2 GeneAnnot ,
3 GeneTide ,
Sets of similar genes according to GeneDecks ,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP ,
plus additional links to
SOURCE , and/or
GNF
BioGPS , and/or
EXPOLDB , and/or
UniProtKB ,
Expression Assays from
Applied Biosystems
)
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TAF12 expression in normal and diseased human tissues Applied Biosystems TaqMan ® Gene Expression Assays for TAF12 1 / 2 / 3
4 probe-sets matching TAF12 gene Data from
(Publications) and GNF BioGPS About these images About these images CGAP SAGE TAG: TGGATCCCAGSOURCE GeneReport for Unigene cluster: Hs.530251 Expression variation in blood from EXPOLDB for TAF12
UniProtKB/Swiss-Prot: TAF12_HUMAN, Q16514 Tissue specificity : Ubiquitous
Orthologs for TAF12
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
and/or
5 MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase ,
Gene Trees according to Ensembl )
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Orthologs for TAF12 gene from 5/14 species (see all 14
)
About this table Species with no ortholog for TAF12 ENSEMBL Gene Tree for TAF12 Paralogs for TAF12 (Paralogs according to 1 HomoloGene and 2 Ensembl , Pseudogenes according to 3 Pseudogene.org )About This Section
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SNPs/Variants for TAF12 (According to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE , and
UniProtKB ,
Linkage Disequilibrium by HapMap ,
Genotyping Reagents from
Applied Biosystems )
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HapMap Linkage Disequilibrium images for TAF12 (up to first 250kb)
Disorders & Mutations for TAF12
(in which this Gene is Involved, According to
OMIM, UniProtKB ,
PharmGKB ,
Genatlas , GeneTests ,
Blood group antigen gene mutations by BGMUT ,
HGMD, GAD ,
HuGE Navigator ,
BCGD ,
and/or TGDB .)
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OMIM: 600773
Medical News for TAF12 (Possibly Related Articles in
Doctor's Guide )
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Publications for TAF12 (in
PubMed .
Associations of this gene to articles via
1 Novoseek ,
2 HGNC ,
3 Entrez Gene ,
4 UniProtKB/Swiss-Prot ,
5 UniProtKB/TrEMBL ,
6 GAD , and/or
7 PharmGKB )
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10/45 PubMed articles for TAF12 gene (see all 45
): Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID. (PubMed id 7729427) 2, 3, 4 Mengus G....Davidson I. (1995) The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs. (PubMed id 10594036) 1, 3, 4 Gangloff Y.-G....Davidson I. (2000) The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414) 3, 4 Gregory S.G.... Bentley D.R. (2006) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 3, 4 Gerhard D.S....Malek J. (2004) Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo. (PubMed id 11564863) 3, 4 Martinez E....Roeder R.G. (2001) Histone-like TAFs within the PCAF histone acetylase complex. (PubMed id 9674425) 3, 4 Ogryzko V.V.... Nakatani Y. (1998) The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily. (PubMed id 9885574) 3, 4 Vassilev A....Nakatani Y. (1998) Isolation and characterization of a cDNA encoding a novel human transcription factor TFIID subunit containing similarities with histones H2B and H3. (PubMed id 8647459) 3, 4 Choi B.... Horikoshi M. (1996) A histone octamer-like structure within TFIID. (PubMed id 8598932) 3, 4 Hoffmann A.... Roeder R.G. (1996) Cloning and characterization of human TAF20/15. Multiple interactions suggest a central role in TFIID complex formation. (PubMed id 8663456) 3, 4 Hoffmann A. and Roeder R.G. (1996)
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AND OR
Aliases
TAF12 (Gene Symbol) OTTHUMP00000003785 OTTHUMP00000003786 TAF15 TAF2J TAFII-20/TAFII-15 TAFII20 TAFII20/TAFII15 TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20 kD TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa TAF12 RNA polymerase II, TATA box binding protein-associated factor, 20kDa TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD Transcription initiation factor TFIID 20/15 kDa subunits
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Genome Databases showing TAF12
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
and/or
H-InvDB )
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Other Databases showing TAF12
(According to HUGE )
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Specialized Databases showing TAF12 (According to ATLAS , HORDE , IMGT , MTDB, LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
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Name Description
NIEHS-SNPs http://egp.gs.washington.edu/data/taf12/
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Signaling Technology , Abcam , Novus Biologicals ,Sigma-Aldrich , R&D Systems , Millipore , Abnova , and/or Invitrogen , Clones available from OriGene ,and/or Invitrogen , Drugs and/or compounds by Sigma-Aldrich , Enzo Life Sciences , and/or Tocris Bioscience )About This Section
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